Rapid screening and identification of ACE inhibitors in snake venoms using at-line nanofractionation LC-MS View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-10

AUTHORS

Marija Mladic, Tessa de Waal, Lindsey Burggraaff, Julien Slagboom, Govert W. Somsen, Wilfried M. A. Niessen, R. Manjunatha Kini, Jeroen Kool

ABSTRACT

This study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ACE) and a strategy for their rapid identification. The method is based on an at-line nanofractionation approach, which combines liquid chromatography (LC), mass spectrometry (MS), and pharmacology in one platform. After initial LC separation of a crude venom, a post-column flow split is introduced enabling parallel MS identification and high-resolution fractionation onto 384-well plates. The plates are subsequently freeze-dried and used in a fluorescence-based ACE activity assay to determine the ability of the nanofractions to inhibit ACE activity. Once the bioactive wells are identified, the parallel MS data reveals the masses corresponding to the activities found. Narrowing down of possible bioactive candidates is provided by comparison of bioactivity profiles after reversed-phase liquid chromatography (RPLC) and after hydrophilic interaction chromatography (HILIC) of a crude venom. Additional nanoLC-MS/MS analysis is performed on the content of the bioactive nanofractions to determine peptide sequences. The method described was optimized, evaluated, and successfully applied for screening of 30 snake venoms for the presence of ACE inhibitors. As a result, two new bioactive peptides were identified: pELWPRPHVPP in Crotalus viridis viridis venom with IC50 = 1.1 μM and pEWPPWPPRPPIPP in Cerastes cerastes cerastes venom with IC50 = 3.5 μM. The identified peptides possess a high sequence similarity to other bradykinin-potentiating peptides (BPPs), which are known ACE inhibitors found in snake venoms. More... »

PAGES

5987-5997

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-017-0531-3

DOI

http://dx.doi.org/10.1007/s00216-017-0531-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091146557

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28801827


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Analytical Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Angiotensin-Converting Enzyme Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chemical Fractionation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Reverse-Phase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crotalid Venoms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Assays", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nanotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptidyl-Dipeptidase A", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Snake Venoms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Snakes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tandem Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viper Venoms", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mladic", 
        "givenName": "Marija", 
        "id": "sg:person.01063217011.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063217011.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Waal", 
        "givenName": "Tessa", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Burggraaff", 
        "givenName": "Lindsey", 
        "id": "sg:person.012250317067.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012250317067.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Slagboom", 
        "givenName": "Julien", 
        "id": "sg:person.014142472757.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014142472757.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Somsen", 
        "givenName": "Govert W.", 
        "id": "sg:person.01211337727.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211337727.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands", 
            "hyphen MassSpec, Herenweg 95, 2361 EK, Warmond, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Niessen", 
        "givenName": "Wilfried M. A.", 
        "id": "sg:person.01315356155.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315356155.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National University of Singapore", 
          "id": "https://www.grid.ac/institutes/grid.4280.e", 
          "name": [
            "Department of Biological Science, National University of Singapore, 14 Science Drive 4, 117543, Singapore, Singapore"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Manjunatha Kini", 
        "givenName": "R.", 
        "id": "sg:person.01022043553.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022043553.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "VU University Amsterdam", 
          "id": "https://www.grid.ac/institutes/grid.12380.38", 
          "name": [
            "Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kool", 
        "givenName": "Jeroen", 
        "id": "sg:person.0611100247.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611100247.78"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0145071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000443581"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-13-312", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002602636", 
          "https://doi.org/10.1186/1471-2164-13-312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rcm.1973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003656019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rcm.1973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003656019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1586/14789450.2015.1073590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003732026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.hyp.17.4.589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004046871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2014.07.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007169360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2015.12.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008518987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2008.02.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010437747"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0009-8981(79)90091-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010559788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0009-8981(79)90091-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010559788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/toxins5020327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010934595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2014.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012302376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-8703(99)70075-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017784742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2013.07.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020624185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1022545020764", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024599832", 
          "https://doi.org/10.1023/a:1022545020764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2006.349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036644035", 
          "https://doi.org/10.1038/nprot.2006.349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2006.349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036644035", 
          "https://doi.org/10.1038/nprot.2006.349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.peptides.2004.04.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046011522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.03-0093life", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049831214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12953-016-0090-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051813242", 
          "https://doi.org/10.1186/s12953-016-0090-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1527-3466.1999.tb00015.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052642507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1527-3466.1999.tb00015.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052642507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00798a004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055188810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00815a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055189341"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijbiomac.2017.01.131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083532305"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-10", 
    "datePublishedReg": "2017-10-01", 
    "description": "This study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ACE) and a strategy for their rapid identification. The method is based on an at-line nanofractionation approach, which combines liquid chromatography (LC), mass spectrometry (MS), and pharmacology in one platform. After initial LC separation of a crude venom, a post-column flow split is introduced enabling parallel MS identification and high-resolution fractionation onto 384-well plates. The plates are subsequently freeze-dried and used in a fluorescence-based ACE activity assay to determine the ability of the nanofractions to inhibit ACE activity. Once the bioactive wells are identified, the parallel MS data reveals the masses corresponding to the activities found. Narrowing down of possible bioactive candidates is provided by comparison of bioactivity profiles after reversed-phase liquid chromatography (RPLC) and after hydrophilic interaction chromatography (HILIC) of a crude venom. Additional nanoLC-MS/MS analysis is performed on the content of the bioactive nanofractions to determine peptide sequences. The method described was optimized, evaluated, and successfully applied for screening of 30 snake venoms for the presence of ACE inhibitors. As a result, two new bioactive peptides were identified: pELWPRPHVPP in Crotalus viridis viridis venom with IC50\u00a0=\u00a01.1\u00a0\u03bcM and pEWPPWPPRPPIPP in Cerastes cerastes cerastes venom with IC50\u00a0=\u00a03.5\u00a0\u03bcM. The identified peptides possess a high sequence similarity to other bradykinin-potentiating peptides (BPPs), which are known ACE inhibitors found in snake venoms.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00216-017-0531-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4138246", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1357342", 
        "issn": [
          "1618-2642", 
          "1618-2650"
        ], 
        "name": "Analytical and Bioanalytical Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "25", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "409"
      }
    ], 
    "name": "Rapid screening and identification of ACE inhibitors in snake venoms using at-line nanofractionation LC-MS", 
    "pagination": "5987-5997", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b7b2fef9ebcc72d2dbe2f81f05a39dea09087c538b5cc416f6afe45c6ccfc233"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28801827"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101134327"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00216-017-0531-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1091146557"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00216-017-0531-3", 
      "https://app.dimensions.ai/details/publication/pub.1091146557"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:38", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113677_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00216-017-0531-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0531-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0531-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0531-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0531-3'


 

This table displays all metadata directly associated to this object as RDF triples.

261 TRIPLES      21 PREDICATES      68 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00216-017-0531-3 schema:about N0036e6337e9547c7bd65b24611dd2160
2 N16c521528c1c4a58962c1700b6d8153d
3 N1cb62aa40f8646a8820003b963414c3f
4 N1e8f1478f17f4b7cad1799ebee9c8592
5 N47f0b33b6b3145f69585e73f700a2348
6 N50d790ca51e44e06a329bf3a223d64c2
7 N7f3b70ab2aad4b6ca59b0f04791bfc80
8 N80bec5158aed47f18a8e3a6480f0144d
9 N843ffe4e71ef4b7781be145c2ea9871a
10 N94beb2665eb74ad3a3e0aff416b208c2
11 Na6b819d8ce29461996011f733ffc70ab
12 Na8c97cf8c0274f259c4bc87f918fcc03
13 Naac236ef756943549a93e219c245befa
14 Nb109b5e590154fc6bef6cb275f728726
15 Nd9ff2603acc04b349baefc8586b551c6
16 Ne19ddcc413d84e4986903c20e54d82da
17 Ne9bd52ec662640b38b55c03d009a4927
18 anzsrc-for:03
19 anzsrc-for:0301
20 schema:author N74a66c9eb9244922b789b8bcf2e93ddf
21 schema:citation sg:pub.10.1023/a:1022545020764
22 sg:pub.10.1038/nprot.2006.349
23 sg:pub.10.1186/1471-2164-13-312
24 sg:pub.10.1186/s12953-016-0090-0
25 https://doi.org/10.1002/rcm.1973
26 https://doi.org/10.1016/0009-8981(79)90091-3
27 https://doi.org/10.1016/j.bcp.2013.07.016
28 https://doi.org/10.1016/j.bcp.2014.07.001
29 https://doi.org/10.1016/j.ijbiomac.2017.01.131
30 https://doi.org/10.1016/j.peptides.2004.04.006
31 https://doi.org/10.1016/j.toxicon.2008.02.019
32 https://doi.org/10.1016/j.toxicon.2014.07.011
33 https://doi.org/10.1016/j.toxicon.2015.12.008
34 https://doi.org/10.1016/s0002-8703(99)70075-x
35 https://doi.org/10.1021/bi00798a004
36 https://doi.org/10.1021/bi00815a005
37 https://doi.org/10.1096/fj.03-0093life
38 https://doi.org/10.1111/j.1527-3466.1999.tb00015.x
39 https://doi.org/10.1161/01.hyp.17.4.589
40 https://doi.org/10.1371/journal.pone.0145071
41 https://doi.org/10.1586/14789450.2015.1073590
42 https://doi.org/10.3390/toxins5020327
43 schema:datePublished 2017-10
44 schema:datePublishedReg 2017-10-01
45 schema:description This study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ACE) and a strategy for their rapid identification. The method is based on an at-line nanofractionation approach, which combines liquid chromatography (LC), mass spectrometry (MS), and pharmacology in one platform. After initial LC separation of a crude venom, a post-column flow split is introduced enabling parallel MS identification and high-resolution fractionation onto 384-well plates. The plates are subsequently freeze-dried and used in a fluorescence-based ACE activity assay to determine the ability of the nanofractions to inhibit ACE activity. Once the bioactive wells are identified, the parallel MS data reveals the masses corresponding to the activities found. Narrowing down of possible bioactive candidates is provided by comparison of bioactivity profiles after reversed-phase liquid chromatography (RPLC) and after hydrophilic interaction chromatography (HILIC) of a crude venom. Additional nanoLC-MS/MS analysis is performed on the content of the bioactive nanofractions to determine peptide sequences. The method described was optimized, evaluated, and successfully applied for screening of 30 snake venoms for the presence of ACE inhibitors. As a result, two new bioactive peptides were identified: pELWPRPHVPP in Crotalus viridis viridis venom with IC<sub>50</sub> = 1.1 μM and pEWPPWPPRPPIPP in Cerastes cerastes cerastes venom with IC<sub>50</sub> = 3.5 μM. The identified peptides possess a high sequence similarity to other bradykinin-potentiating peptides (BPPs), which are known ACE inhibitors found in snake venoms.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf Nb9dc43422b8b4dd887970272249a6b45
50 Nc7f1b309ff85400189eee257ab816e9e
51 sg:journal.1357342
52 schema:name Rapid screening and identification of ACE inhibitors in snake venoms using at-line nanofractionation LC-MS
53 schema:pagination 5987-5997
54 schema:productId N07c900a3195a4ab7af94b3b9f5a47abf
55 N60eeacf467614e6b8b8b7b04ec03c0b6
56 Na063f1c9aefb45a48739775117ecd2e8
57 Ndbc3827b528f459e8a5d1793461cb6fa
58 Nf07b3a5f7e664f8a9ccb7dff4f6def59
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091146557
60 https://doi.org/10.1007/s00216-017-0531-3
61 schema:sdDatePublished 2019-04-11T10:38
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher N6a352f9a141c4d6287f79bbfb9a6b9ad
64 schema:url https://link.springer.com/10.1007%2Fs00216-017-0531-3
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N0036e6337e9547c7bd65b24611dd2160 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Nanotechnology
70 rdf:type schema:DefinedTerm
71 N07c900a3195a4ab7af94b3b9f5a47abf schema:name readcube_id
72 schema:value b7b2fef9ebcc72d2dbe2f81f05a39dea09087c538b5cc416f6afe45c6ccfc233
73 rdf:type schema:PropertyValue
74 N16c521528c1c4a58962c1700b6d8153d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Animals
76 rdf:type schema:DefinedTerm
77 N1cb62aa40f8646a8820003b963414c3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Tandem Mass Spectrometry
79 rdf:type schema:DefinedTerm
80 N1e8f1478f17f4b7cad1799ebee9c8592 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Rabbits
82 rdf:type schema:DefinedTerm
83 N3567d7593c4242d38735c9a5c3207eb0 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
84 schema:familyName de Waal
85 schema:givenName Tessa
86 rdf:type schema:Person
87 N3f2c6453386648ff9c21b0da7fd0df4e rdf:first sg:person.01211337727.06
88 rdf:rest Nf6ac9d4f1f9b418ea775216ce1e52ca2
89 N47f0b33b6b3145f69585e73f700a2348 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Enzyme Assays
91 rdf:type schema:DefinedTerm
92 N50d790ca51e44e06a329bf3a223d64c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Chromatography, Reverse-Phase
94 rdf:type schema:DefinedTerm
95 N60eeacf467614e6b8b8b7b04ec03c0b6 schema:name pubmed_id
96 schema:value 28801827
97 rdf:type schema:PropertyValue
98 N67a337e0c4564b1793e9d68e49593fe3 rdf:first N3567d7593c4242d38735c9a5c3207eb0
99 rdf:rest Nbd35fae662034b52b6f251668627c0ce
100 N6a352f9a141c4d6287f79bbfb9a6b9ad schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 N71c1d435bf804cb9abe5f60fc3c1737a rdf:first sg:person.014142472757.88
103 rdf:rest N3f2c6453386648ff9c21b0da7fd0df4e
104 N74a66c9eb9244922b789b8bcf2e93ddf rdf:first sg:person.01063217011.54
105 rdf:rest N67a337e0c4564b1793e9d68e49593fe3
106 N7f3b70ab2aad4b6ca59b0f04791bfc80 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Peptides
108 rdf:type schema:DefinedTerm
109 N80bec5158aed47f18a8e3a6480f0144d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Viper Venoms
111 rdf:type schema:DefinedTerm
112 N843ffe4e71ef4b7781be145c2ea9871a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Chemical Fractionation
114 rdf:type schema:DefinedTerm
115 N94beb2665eb74ad3a3e0aff416b208c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Amino Acid Sequence
117 rdf:type schema:DefinedTerm
118 Na063f1c9aefb45a48739775117ecd2e8 schema:name doi
119 schema:value 10.1007/s00216-017-0531-3
120 rdf:type schema:PropertyValue
121 Na68dfdc8dea24d55b3d15948afa9841b rdf:first sg:person.0611100247.78
122 rdf:rest rdf:nil
123 Na6b819d8ce29461996011f733ffc70ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Snakes
125 rdf:type schema:DefinedTerm
126 Na8c97cf8c0274f259c4bc87f918fcc03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Angiotensin-Converting Enzyme Inhibitors
128 rdf:type schema:DefinedTerm
129 Naac236ef756943549a93e219c245befa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Mass Spectrometry
131 rdf:type schema:DefinedTerm
132 Nb109b5e590154fc6bef6cb275f728726 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Chromatography, Liquid
134 rdf:type schema:DefinedTerm
135 Nb9dc43422b8b4dd887970272249a6b45 schema:volumeNumber 409
136 rdf:type schema:PublicationVolume
137 Nbd35fae662034b52b6f251668627c0ce rdf:first sg:person.012250317067.70
138 rdf:rest N71c1d435bf804cb9abe5f60fc3c1737a
139 Nc5089571b4a540d0a3bd66cafd24f2dc rdf:first sg:person.01022043553.80
140 rdf:rest Na68dfdc8dea24d55b3d15948afa9841b
141 Nc7f1b309ff85400189eee257ab816e9e schema:issueNumber 25
142 rdf:type schema:PublicationIssue
143 Nd9ff2603acc04b349baefc8586b551c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Peptidyl-Dipeptidase A
145 rdf:type schema:DefinedTerm
146 Ndbc3827b528f459e8a5d1793461cb6fa schema:name dimensions_id
147 schema:value pub.1091146557
148 rdf:type schema:PropertyValue
149 Ne19ddcc413d84e4986903c20e54d82da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Snake Venoms
151 rdf:type schema:DefinedTerm
152 Ne9bd52ec662640b38b55c03d009a4927 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Crotalid Venoms
154 rdf:type schema:DefinedTerm
155 Nf07b3a5f7e664f8a9ccb7dff4f6def59 schema:name nlm_unique_id
156 schema:value 101134327
157 rdf:type schema:PropertyValue
158 Nf6ac9d4f1f9b418ea775216ce1e52ca2 rdf:first sg:person.01315356155.22
159 rdf:rest Nc5089571b4a540d0a3bd66cafd24f2dc
160 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
161 schema:name Chemical Sciences
162 rdf:type schema:DefinedTerm
163 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
164 schema:name Analytical Chemistry
165 rdf:type schema:DefinedTerm
166 sg:grant.4138246 http://pending.schema.org/fundedItem sg:pub.10.1007/s00216-017-0531-3
167 rdf:type schema:MonetaryGrant
168 sg:journal.1357342 schema:issn 1618-2642
169 1618-2650
170 schema:name Analytical and Bioanalytical Chemistry
171 rdf:type schema:Periodical
172 sg:person.01022043553.80 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
173 schema:familyName Manjunatha Kini
174 schema:givenName R.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022043553.80
176 rdf:type schema:Person
177 sg:person.01063217011.54 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
178 schema:familyName Mladic
179 schema:givenName Marija
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063217011.54
181 rdf:type schema:Person
182 sg:person.01211337727.06 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
183 schema:familyName Somsen
184 schema:givenName Govert W.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211337727.06
186 rdf:type schema:Person
187 sg:person.012250317067.70 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
188 schema:familyName Burggraaff
189 schema:givenName Lindsey
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012250317067.70
191 rdf:type schema:Person
192 sg:person.01315356155.22 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
193 schema:familyName Niessen
194 schema:givenName Wilfried M. A.
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315356155.22
196 rdf:type schema:Person
197 sg:person.014142472757.88 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
198 schema:familyName Slagboom
199 schema:givenName Julien
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014142472757.88
201 rdf:type schema:Person
202 sg:person.0611100247.78 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
203 schema:familyName Kool
204 schema:givenName Jeroen
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611100247.78
206 rdf:type schema:Person
207 sg:pub.10.1023/a:1022545020764 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024599832
208 https://doi.org/10.1023/a:1022545020764
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/nprot.2006.349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036644035
211 https://doi.org/10.1038/nprot.2006.349
212 rdf:type schema:CreativeWork
213 sg:pub.10.1186/1471-2164-13-312 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002602636
214 https://doi.org/10.1186/1471-2164-13-312
215 rdf:type schema:CreativeWork
216 sg:pub.10.1186/s12953-016-0090-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051813242
217 https://doi.org/10.1186/s12953-016-0090-0
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1002/rcm.1973 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003656019
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/0009-8981(79)90091-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010559788
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.bcp.2013.07.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020624185
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.bcp.2014.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012302376
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/j.ijbiomac.2017.01.131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083532305
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/j.peptides.2004.04.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046011522
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/j.toxicon.2008.02.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010437747
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/j.toxicon.2014.07.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007169360
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/j.toxicon.2015.12.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008518987
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0002-8703(99)70075-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017784742
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1021/bi00798a004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055188810
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1021/bi00815a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055189341
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1096/fj.03-0093life schema:sameAs https://app.dimensions.ai/details/publication/pub.1049831214
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1111/j.1527-3466.1999.tb00015.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052642507
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1161/01.hyp.17.4.589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004046871
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1371/journal.pone.0145071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000443581
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1586/14789450.2015.1073590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003732026
252 rdf:type schema:CreativeWork
253 https://doi.org/10.3390/toxins5020327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010934595
254 rdf:type schema:CreativeWork
255 https://www.grid.ac/institutes/grid.12380.38 schema:alternateName VU University Amsterdam
256 schema:name Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands
257 hyphen MassSpec, Herenweg 95, 2361 EK, Warmond, The Netherlands
258 rdf:type schema:Organization
259 https://www.grid.ac/institutes/grid.4280.e schema:alternateName National University of Singapore
260 schema:name Department of Biological Science, National University of Singapore, 14 Science Drive 4, 117543, Singapore, Singapore
261 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...