Single-shot multi-reaction monitoring of intact marker conjugates for quantitative profiling of human major microsomal glucuronidations and its utility to screen ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-11

AUTHORS

Yuqing Cheng, Jing Zhou, Meiling Wang, Yanyan Liu, Bin Guo, Bo Chen

ABSTRACT

UDP-glucuronosyltransferase (UGT) is a polymorphic family of conjugating enzymes responsible for the elimination of a myriad of xenobiotics and endogenous compounds. The precise reaction phenotyping of this multi-isoform superfamily is hampered by a lack of fast generic methods for directly measuring the diverse glucuronoconjugate metabolites for comprehensive profiling of UGT isoform-specific glucuronidations. We report here a single-shot liquid chromatography-tandem mass spectrometry (LC-MS/MS) method enabling the simultaneous direct measurement of nine intact glucuronides from hepatic microsomal glucuronidations mediated by a battery of isoforms (1A1, 1A3, 1A4, 1A6, 1A9, 2B7, 2B10, 2B15, and 2B17), which represent the majority of human UGTs in drug metabolism. This new method is based on post-incubation pooling of the individual probe reaction samples for nine-in-one cassette analysis with polarity switching multiple reaction monitoring (MRM) of all the marker glucuronides within a single LC-MS/MS injection. The pooled sample strategy overcomes the cross-interferences among the cocktail substrates and also increases the throughput. The periodic polarity switching of the LC-MRM acquisition expands the glucuronide profiling coverage using a generic single-run analysis. The source-induced dissociation of the glucuronoconjugates was evaluated as a generic alternative for their quantitation as their free aglycones, but a significant bias occurs against the traditional assumption that the parent substrates could be used as the surrogates for quantifying their glucuronide metabolites without authentic standards. After collective validations for analyte quantitation and enzyme kinetics, this single-shot cassette quantitative profiling approach may prove useful in large-scale phenotyping of human glucuronidations and rapid screening for UGT inhibitors in natural products. Graphical abstract Multi-reaction monitoring of intact conjugate metabolites for quantitative profiling of human major glucuronidations. More... »

PAGES

8117-8132

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-016-9915-z

DOI

http://dx.doi.org/10.1007/s00216-016-9915-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044219237

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27604270


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Analytical Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Monitoring", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drugs, Chinese Herbal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucuronides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucuronosyltransferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microsomes, Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tandem Mass Spectrometry", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, 410081, Changsha, Hunan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheng", 
        "givenName": "Yuqing", 
        "id": "sg:person.011337571471.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011337571471.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University, 410081, Changsha, Hunan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Jing", 
        "id": "sg:person.01227263224.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227263224.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Central South University", 
          "id": "https://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "College of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, Hunan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Meiling", 
        "id": "sg:person.01044721424.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044721424.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University, 410081, Changsha, Hunan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Yanyan", 
        "id": "sg:person.014035322105.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014035322105.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, 410081, Changsha, Hunan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guo", 
        "givenName": "Bin", 
        "id": "sg:person.01171300737.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171300737.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, 410081, Changsha, Hunan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Bo", 
        "id": "sg:person.01300653320.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300653320.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1124/dmd.104.000794", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000366208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.108.024844", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001434080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rcm.1071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001928782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-009-3441-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004474763", 
          "https://doi.org/10.1007/s00216-009-3441-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-009-3441-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004474763", 
          "https://doi.org/10.1007/s00216-009-3441-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep09627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005090368", 
          "https://doi.org/10.1038/srep09627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep09627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005090368", 
          "https://doi.org/10.1038/srep09627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1556-276x-9-642", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008979672", 
          "https://doi.org/10.1186/1556-276x-9-642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.113.056291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009715941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rcm.7030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014254188"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rcm.4558", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014945766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bdd.1933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015134401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3np70003h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019216037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3cc38810g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019286473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dmpk.2014.12.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021276172"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.steroids.2012.02.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021781306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.105.007369", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022983412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.113.051656", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025009357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pharmthera.2013.09.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025349540"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-7843.2008.00247.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025565431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13361-011-0180-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026356841", 
          "https://doi.org/10.1007/s13361-011-0180-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bmc.1572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026482124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bmc.1572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026482124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.199520531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027062165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.107.037093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028412890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.111.043117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029081167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.31.9.1125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032298546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/00498254.2015.1036954", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032888346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jpba.2011.03.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033527959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.114.058818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033852957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jms.1914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034842586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2012.04.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036226069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.31.1.133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036586668"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.286.5439.487", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038308932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ddtec.2006.03.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038682378"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jchromb.2014.07.037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038715675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/clpt.1992.42", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042448233", 
          "https://doi.org/10.1038/clpt.1992.42"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chroma.2010.05.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043107330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ejps.2015.02.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045971596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.111.040865", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046659923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.30.11.1257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046798162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/03602530903210682", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048328006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/dmd.114.063016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053190717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac4001749", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055004032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac504118y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055019975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac702613k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055069725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac702613k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055069725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac901320z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055071497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac901320z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055071497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.jafc.5b02222", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055095108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp060021l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056212195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp060021l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056212195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp900065b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056214075"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp900065b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056214075"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/tx300339u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056299001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/138920010792927343", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069175927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/138920012803341401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069176150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/187231207779814364", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069225484"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-11", 
    "datePublishedReg": "2016-11-01", 
    "description": "UDP-glucuronosyltransferase (UGT) is a polymorphic family of conjugating enzymes responsible for the elimination of a myriad of xenobiotics and endogenous compounds. The precise reaction phenotyping of this multi-isoform superfamily is hampered by a lack of fast generic methods for directly measuring the diverse glucuronoconjugate metabolites for comprehensive profiling of UGT isoform-specific glucuronidations. We report here a single-shot liquid chromatography-tandem mass spectrometry (LC-MS/MS) method enabling the simultaneous direct measurement of nine intact glucuronides from hepatic microsomal glucuronidations mediated by a battery of isoforms (1A1, 1A3, 1A4, 1A6, 1A9, 2B7, 2B10, 2B15, and 2B17), which represent the majority of human UGTs in drug metabolism. This new method is based on post-incubation pooling of the individual probe reaction samples for nine-in-one cassette analysis with polarity switching multiple reaction monitoring (MRM) of all the marker glucuronides within a single LC-MS/MS injection. The pooled sample strategy overcomes the cross-interferences among the cocktail substrates and also increases the throughput. The periodic polarity switching of the LC-MRM acquisition expands the glucuronide profiling coverage using a generic single-run analysis. The source-induced dissociation of the glucuronoconjugates was evaluated as a generic alternative for their quantitation as their free aglycones, but a significant bias occurs against the traditional assumption that the parent substrates could be used as the surrogates for quantifying their glucuronide metabolites without authentic standards. After collective validations for analyte quantitation and enzyme kinetics, this single-shot cassette quantitative profiling approach may prove useful in large-scale phenotyping of human glucuronidations and rapid screening for UGT inhibitors in natural products. Graphical abstract Multi-reaction monitoring of intact conjugate metabolites for quantitative profiling of human major glucuronidations.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00216-016-9915-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1357342", 
        "issn": [
          "1618-2642", 
          "1618-2650"
        ], 
        "name": "Analytical and Bioanalytical Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "28", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "408"
      }
    ], 
    "name": "Single-shot multi-reaction monitoring of intact marker conjugates for quantitative profiling of human major microsomal glucuronidations and its utility to screen inhibitors from medicinal herbs", 
    "pagination": "8117-8132", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d2a133c5b300e552c8c34070273497cf3de04ed4d28eabe13f31455e4cae7de9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27604270"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101134327"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00216-016-9915-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044219237"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00216-016-9915-z", 
      "https://app.dimensions.ai/details/publication/pub.1044219237"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70043_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00216-016-9915-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9915-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9915-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9915-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9915-z'


 

This table displays all metadata directly associated to this object as RDF triples.

314 TRIPLES      21 PREDICATES      92 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00216-016-9915-z schema:about N12bf9dfeb34444c18e5141d82470a4b6
2 N1f3cfc86858248dea9471c330009909b
3 N2d68317bd58f4e0d92a58f79bd75e70f
4 N33e1caac77dd4b8589181b99350397ba
5 N35e1f70199574ec385e5652827e42896
6 N3750ebfc99a34e5585a60e4e75b2905e
7 N413f197a983244568532fe18dc035da2
8 N44555efb54204f9e82ad067882d09554
9 N51b346ce7558417e95eef0cb4e2ed3ce
10 N5b2af59fc715461292b0e5769318a325
11 Nc22289d335c94c8ca167e1ea4dbfb62b
12 Nd0276d70720549a3ac88579d9b16c43e
13 anzsrc-for:03
14 anzsrc-for:0301
15 schema:author Ne518e810eb1e4f84b1552b7f3e5eed1d
16 schema:citation sg:pub.10.1007/s00216-009-3441-1
17 sg:pub.10.1007/s13361-011-0180-2
18 sg:pub.10.1038/clpt.1992.42
19 sg:pub.10.1038/srep09627
20 sg:pub.10.1186/1556-276x-9-642
21 https://doi.org/10.1002/anie.199520531
22 https://doi.org/10.1002/bdd.1933
23 https://doi.org/10.1002/bmc.1572
24 https://doi.org/10.1002/jms.1914
25 https://doi.org/10.1002/rcm.1071
26 https://doi.org/10.1002/rcm.4558
27 https://doi.org/10.1002/rcm.7030
28 https://doi.org/10.1016/j.ab.2012.04.031
29 https://doi.org/10.1016/j.chroma.2010.05.025
30 https://doi.org/10.1016/j.ddtec.2006.03.007
31 https://doi.org/10.1016/j.dmpk.2014.12.001
32 https://doi.org/10.1016/j.ejps.2015.02.001
33 https://doi.org/10.1016/j.jchromb.2014.07.037
34 https://doi.org/10.1016/j.jpba.2011.03.034
35 https://doi.org/10.1016/j.pharmthera.2013.09.002
36 https://doi.org/10.1016/j.steroids.2012.02.023
37 https://doi.org/10.1021/ac4001749
38 https://doi.org/10.1021/ac504118y
39 https://doi.org/10.1021/ac702613k
40 https://doi.org/10.1021/ac901320z
41 https://doi.org/10.1021/acs.jafc.5b02222
42 https://doi.org/10.1021/mp060021l
43 https://doi.org/10.1021/mp900065b
44 https://doi.org/10.1021/tx300339u
45 https://doi.org/10.1039/c3cc38810g
46 https://doi.org/10.1039/c3np70003h
47 https://doi.org/10.1111/j.1742-7843.2008.00247.x
48 https://doi.org/10.1124/dmd.104.000794
49 https://doi.org/10.1124/dmd.105.007369
50 https://doi.org/10.1124/dmd.108.024844
51 https://doi.org/10.1124/dmd.111.040865
52 https://doi.org/10.1124/dmd.111.043117
53 https://doi.org/10.1124/dmd.113.051656
54 https://doi.org/10.1124/dmd.113.056291
55 https://doi.org/10.1124/dmd.114.058818
56 https://doi.org/10.1124/dmd.114.063016
57 https://doi.org/10.1124/dmd.30.11.1257
58 https://doi.org/10.1124/dmd.31.1.133
59 https://doi.org/10.1124/dmd.31.9.1125
60 https://doi.org/10.1124/mol.107.037093
61 https://doi.org/10.1126/science.286.5439.487
62 https://doi.org/10.2174/138920010792927343
63 https://doi.org/10.2174/138920012803341401
64 https://doi.org/10.2174/187231207779814364
65 https://doi.org/10.3109/00498254.2015.1036954
66 https://doi.org/10.3109/03602530903210682
67 schema:datePublished 2016-11
68 schema:datePublishedReg 2016-11-01
69 schema:description UDP-glucuronosyltransferase (UGT) is a polymorphic family of conjugating enzymes responsible for the elimination of a myriad of xenobiotics and endogenous compounds. The precise reaction phenotyping of this multi-isoform superfamily is hampered by a lack of fast generic methods for directly measuring the diverse glucuronoconjugate metabolites for comprehensive profiling of UGT isoform-specific glucuronidations. We report here a single-shot liquid chromatography-tandem mass spectrometry (LC-MS/MS) method enabling the simultaneous direct measurement of nine intact glucuronides from hepatic microsomal glucuronidations mediated by a battery of isoforms (1A1, 1A3, 1A4, 1A6, 1A9, 2B7, 2B10, 2B15, and 2B17), which represent the majority of human UGTs in drug metabolism. This new method is based on post-incubation pooling of the individual probe reaction samples for nine-in-one cassette analysis with polarity switching multiple reaction monitoring (MRM) of all the marker glucuronides within a single LC-MS/MS injection. The pooled sample strategy overcomes the cross-interferences among the cocktail substrates and also increases the throughput. The periodic polarity switching of the LC-MRM acquisition expands the glucuronide profiling coverage using a generic single-run analysis. The source-induced dissociation of the glucuronoconjugates was evaluated as a generic alternative for their quantitation as their free aglycones, but a significant bias occurs against the traditional assumption that the parent substrates could be used as the surrogates for quantifying their glucuronide metabolites without authentic standards. After collective validations for analyte quantitation and enzyme kinetics, this single-shot cassette quantitative profiling approach may prove useful in large-scale phenotyping of human glucuronidations and rapid screening for UGT inhibitors in natural products. Graphical abstract Multi-reaction monitoring of intact conjugate metabolites for quantitative profiling of human major glucuronidations.
70 schema:genre research_article
71 schema:inLanguage en
72 schema:isAccessibleForFree false
73 schema:isPartOf N65b64e487c444e2586624eebb7162db4
74 N7323b8a84fec43e897412ea597fc92a1
75 sg:journal.1357342
76 schema:name Single-shot multi-reaction monitoring of intact marker conjugates for quantitative profiling of human major microsomal glucuronidations and its utility to screen inhibitors from medicinal herbs
77 schema:pagination 8117-8132
78 schema:productId N77e2a4fdde2b4e33b2cf9050f56aecd2
79 N820597d5873d4be1aa38f96850d1f715
80 N8a545e5525304792b23bea61a4bc7b3f
81 N8c2e13e15be0431bacfed8021e0e1406
82 Naf1415ebc8ee4197beffc88cbbde2ffd
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044219237
84 https://doi.org/10.1007/s00216-016-9915-z
85 schema:sdDatePublished 2019-04-11T12:39
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher N8e7d1c2a5e7e48cda36c715bcbbff636
88 schema:url https://link.springer.com/10.1007%2Fs00216-016-9915-z
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N0268d49499314a449fea1d347490fae1 rdf:first sg:person.01044721424.48
93 rdf:rest Nbd67bfa9fee2435b8a1596607f1f675b
94 N12bf9dfeb34444c18e5141d82470a4b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Chromatography, Liquid
96 rdf:type schema:DefinedTerm
97 N1f3cfc86858248dea9471c330009909b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Biomarkers
99 rdf:type schema:DefinedTerm
100 N2d68317bd58f4e0d92a58f79bd75e70f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name In Vitro Techniques
102 rdf:type schema:DefinedTerm
103 N33e1caac77dd4b8589181b99350397ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Enzyme Inhibitors
105 rdf:type schema:DefinedTerm
106 N35e1f70199574ec385e5652827e42896 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Tandem Mass Spectrometry
108 rdf:type schema:DefinedTerm
109 N3750ebfc99a34e5585a60e4e75b2905e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Drug Monitoring
111 rdf:type schema:DefinedTerm
112 N413f197a983244568532fe18dc035da2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Humans
114 rdf:type schema:DefinedTerm
115 N44555efb54204f9e82ad067882d09554 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Substrate Specificity
117 rdf:type schema:DefinedTerm
118 N51b346ce7558417e95eef0cb4e2ed3ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Microsomes, Liver
120 rdf:type schema:DefinedTerm
121 N561cc8200d6942d9bef05fb801316469 rdf:first sg:person.01171300737.15
122 rdf:rest N8c7b16de6c94496a93ef48ec87368338
123 N5b2af59fc715461292b0e5769318a325 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Drugs, Chinese Herbal
125 rdf:type schema:DefinedTerm
126 N65b64e487c444e2586624eebb7162db4 schema:issueNumber 28
127 rdf:type schema:PublicationIssue
128 N7323b8a84fec43e897412ea597fc92a1 schema:volumeNumber 408
129 rdf:type schema:PublicationVolume
130 N77e2a4fdde2b4e33b2cf9050f56aecd2 schema:name nlm_unique_id
131 schema:value 101134327
132 rdf:type schema:PropertyValue
133 N7ed9ab9aa2b44f47ab0697cb9520d94e rdf:first sg:person.01227263224.21
134 rdf:rest N0268d49499314a449fea1d347490fae1
135 N820597d5873d4be1aa38f96850d1f715 schema:name pubmed_id
136 schema:value 27604270
137 rdf:type schema:PropertyValue
138 N8a545e5525304792b23bea61a4bc7b3f schema:name doi
139 schema:value 10.1007/s00216-016-9915-z
140 rdf:type schema:PropertyValue
141 N8c2e13e15be0431bacfed8021e0e1406 schema:name readcube_id
142 schema:value d2a133c5b300e552c8c34070273497cf3de04ed4d28eabe13f31455e4cae7de9
143 rdf:type schema:PropertyValue
144 N8c7b16de6c94496a93ef48ec87368338 rdf:first sg:person.01300653320.29
145 rdf:rest rdf:nil
146 N8e7d1c2a5e7e48cda36c715bcbbff636 schema:name Springer Nature - SN SciGraph project
147 rdf:type schema:Organization
148 Naf1415ebc8ee4197beffc88cbbde2ffd schema:name dimensions_id
149 schema:value pub.1044219237
150 rdf:type schema:PropertyValue
151 Nbd67bfa9fee2435b8a1596607f1f675b rdf:first sg:person.014035322105.29
152 rdf:rest N561cc8200d6942d9bef05fb801316469
153 Nc22289d335c94c8ca167e1ea4dbfb62b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Glucuronosyltransferase
155 rdf:type schema:DefinedTerm
156 Nd0276d70720549a3ac88579d9b16c43e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Glucuronides
158 rdf:type schema:DefinedTerm
159 Ne518e810eb1e4f84b1552b7f3e5eed1d rdf:first sg:person.011337571471.12
160 rdf:rest N7ed9ab9aa2b44f47ab0697cb9520d94e
161 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
162 schema:name Chemical Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
165 schema:name Analytical Chemistry
166 rdf:type schema:DefinedTerm
167 sg:journal.1357342 schema:issn 1618-2642
168 1618-2650
169 schema:name Analytical and Bioanalytical Chemistry
170 rdf:type schema:Periodical
171 sg:person.01044721424.48 schema:affiliation https://www.grid.ac/institutes/grid.216417.7
172 schema:familyName Wang
173 schema:givenName Meiling
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044721424.48
175 rdf:type schema:Person
176 sg:person.011337571471.12 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
177 schema:familyName Cheng
178 schema:givenName Yuqing
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011337571471.12
180 rdf:type schema:Person
181 sg:person.01171300737.15 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
182 schema:familyName Guo
183 schema:givenName Bin
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171300737.15
185 rdf:type schema:Person
186 sg:person.01227263224.21 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
187 schema:familyName Zhou
188 schema:givenName Jing
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227263224.21
190 rdf:type schema:Person
191 sg:person.01300653320.29 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
192 schema:familyName Chen
193 schema:givenName Bo
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300653320.29
195 rdf:type schema:Person
196 sg:person.014035322105.29 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
197 schema:familyName Liu
198 schema:givenName Yanyan
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014035322105.29
200 rdf:type schema:Person
201 sg:pub.10.1007/s00216-009-3441-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004474763
202 https://doi.org/10.1007/s00216-009-3441-1
203 rdf:type schema:CreativeWork
204 sg:pub.10.1007/s13361-011-0180-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026356841
205 https://doi.org/10.1007/s13361-011-0180-2
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/clpt.1992.42 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042448233
208 https://doi.org/10.1038/clpt.1992.42
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/srep09627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005090368
211 https://doi.org/10.1038/srep09627
212 rdf:type schema:CreativeWork
213 sg:pub.10.1186/1556-276x-9-642 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008979672
214 https://doi.org/10.1186/1556-276x-9-642
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1002/anie.199520531 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027062165
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1002/bdd.1933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015134401
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1002/bmc.1572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026482124
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1002/jms.1914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034842586
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1002/rcm.1071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001928782
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1002/rcm.4558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014945766
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1002/rcm.7030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014254188
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.ab.2012.04.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036226069
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.chroma.2010.05.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043107330
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/j.ddtec.2006.03.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038682378
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.dmpk.2014.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021276172
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.ejps.2015.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045971596
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.jchromb.2014.07.037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038715675
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.jpba.2011.03.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033527959
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.pharmthera.2013.09.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025349540
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.steroids.2012.02.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021781306
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1021/ac4001749 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055004032
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1021/ac504118y schema:sameAs https://app.dimensions.ai/details/publication/pub.1055019975
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1021/ac702613k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055069725
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1021/ac901320z schema:sameAs https://app.dimensions.ai/details/publication/pub.1055071497
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1021/acs.jafc.5b02222 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055095108
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/mp060021l schema:sameAs https://app.dimensions.ai/details/publication/pub.1056212195
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1021/mp900065b schema:sameAs https://app.dimensions.ai/details/publication/pub.1056214075
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1021/tx300339u schema:sameAs https://app.dimensions.ai/details/publication/pub.1056299001
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1039/c3cc38810g schema:sameAs https://app.dimensions.ai/details/publication/pub.1019286473
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1039/c3np70003h schema:sameAs https://app.dimensions.ai/details/publication/pub.1019216037
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1111/j.1742-7843.2008.00247.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025565431
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1124/dmd.104.000794 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000366208
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1124/dmd.105.007369 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022983412
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1124/dmd.108.024844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001434080
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1124/dmd.111.040865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046659923
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1124/dmd.111.043117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029081167
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1124/dmd.113.051656 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025009357
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1124/dmd.113.056291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009715941
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1124/dmd.114.058818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033852957
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1124/dmd.114.063016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053190717
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1124/dmd.30.11.1257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046798162
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1124/dmd.31.1.133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036586668
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1124/dmd.31.9.1125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032298546
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1124/mol.107.037093 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028412890
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1126/science.286.5439.487 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038308932
297 rdf:type schema:CreativeWork
298 https://doi.org/10.2174/138920010792927343 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069175927
299 rdf:type schema:CreativeWork
300 https://doi.org/10.2174/138920012803341401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069176150
301 rdf:type schema:CreativeWork
302 https://doi.org/10.2174/187231207779814364 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069225484
303 rdf:type schema:CreativeWork
304 https://doi.org/10.3109/00498254.2015.1036954 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032888346
305 rdf:type schema:CreativeWork
306 https://doi.org/10.3109/03602530903210682 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048328006
307 rdf:type schema:CreativeWork
308 https://www.grid.ac/institutes/grid.216417.7 schema:alternateName Central South University
309 schema:name College of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, Hunan, China
310 rdf:type schema:Organization
311 https://www.grid.ac/institutes/grid.411427.5 schema:alternateName Hunan Normal University
312 schema:name Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University, 410081, Changsha, Hunan, China
313 Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, 410081, Changsha, Hunan, China
314 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...