Label-free electrochemical genosensor based on mesoporous silica thin film View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-10

AUTHORS

Maroua Saadaoui, Iñigo Fernández, Gema Luna, Paula Díez, Susana Campuzano, Noureddine Raouafi, Alfredo Sánchez, José M. Pingarrón, Reynaldo Villalonga

ABSTRACT

A novel label-free electrochemical strategy for nucleic acid detection was developed by using gold electrodes coated with mesoporous silica thin films as sensing interface. The biosensing approach relies on the covalent attachment of a capture DNA probe on the surface of the silica nanopores and further hybridization with its complementary target oligonucleotide sequence, causing a diffusion hindering of an Fe(CN)6 (3-/4-) electrochemical probe through the nanochannels of the mesoporous film. This DNA-mesoporous silica thin film-modified electrodes allowed sensitive (91.7 A/M) and rapid (45 min) detection of low nanomolar levels of synthetic target DNA (25 fmol) and were successfully employed to quantify the endogenous content of Escherichia coli 16S ribosomal RNA (rRNA) directly in raw bacterial lysate samples without isolation or purification steps. Moreover, the 1-month stability demonstrated by these biosensing devices enables their advanced preparation and storage, as desired for practical real-life applications. Graphical abstract Mesoporous silica thin films as scaffolds for the development of novel label-free electrochemical genosensors to perform selective, sensitive and rapid detection of target oligonucleotide sequences. Application towards E. coli determination. More... »

PAGES

7321-7327

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-016-9608-7

DOI

http://dx.doi.org/10.1007/s00216-016-9608-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040288074

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27236313


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0306", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Chemistry (incl. Structural)", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biosensing Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrochemical Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrodes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Equipment Design", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immobilized Nucleic Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Porosity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Ribosomal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Silicon Dioxide", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Tunis El Manar University", 
          "id": "https://www.grid.ac/institutes/grid.12574.35", 
          "name": [
            "Department of Chemistry, Faculty of Sciences, University of Tunis El Manar, Rue B\u00e9chir Salem Belkheria, Tunis El-Manar, 2092, Tunis, Tunisia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saadaoui", 
        "givenName": "Maroua", 
        "id": "sg:person.01313312101.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313312101.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fern\u00e1ndez", 
        "givenName": "I\u00f1igo", 
        "id": "sg:person.01300744325.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300744325.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Luna", 
        "givenName": "Gema", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "D\u00edez", 
        "givenName": "Paula", 
        "id": "sg:person.015252373020.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015252373020.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Campuzano", 
        "givenName": "Susana", 
        "id": "sg:person.01037305730.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037305730.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tunis El Manar University", 
          "id": "https://www.grid.ac/institutes/grid.12574.35", 
          "name": [
            "Department of Chemistry, Faculty of Sciences, University of Tunis El Manar, Rue B\u00e9chir Salem Belkheria, Tunis El-Manar, 2092, Tunis, Tunisia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raouafi", 
        "givenName": "Noureddine", 
        "id": "sg:person.011213267641.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011213267641.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "S\u00e1nchez", 
        "givenName": "Alfredo", 
        "id": "sg:person.01224045767.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224045767.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pingarr\u00f3n", 
        "givenName": "Jos\u00e9 M.", 
        "id": "sg:person.0627636725.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627636725.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Complutense University of Madrid", 
          "id": "https://www.grid.ac/institutes/grid.4795.f", 
          "name": [
            "Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Villalonga", 
        "givenName": "Reynaldo", 
        "id": "sg:person.01217026335.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217026335.99"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.snb.2014.10.043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001142944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.talanta.2013.03.061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005034686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2014.04.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007046983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.electacta.2014.01.074", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011272614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.nantod.2014.04.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012294451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cphc.201300045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013521922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.elecom.2015.06.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013713125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2015.05.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019735569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.trac.2008.03.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027182617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-014-1301-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029175730", 
          "https://doi.org/10.1007/s00604-014-1301-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10008-014-2570-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029461657", 
          "https://doi.org/10.1007/s10008-014-2570-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-015-1656-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030526804", 
          "https://doi.org/10.1007/s00604-015-1656-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.snb.2015.08.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031775570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3cs60141b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032216608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.trac.2012.03.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034322486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac500618v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034492591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr500100j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034632267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/07388551.2013.778228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035705785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c4cc05083e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037416453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2014.09.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039817760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.44.2.561-570.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041554033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2014.07.078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043609454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.electacta.2013.12.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044639982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2013.05.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048279983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmat1951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051418404", 
          "https://doi.org/10.1038/nmat1951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms1035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053365240", 
          "https://doi.org/10.1038/ncomms1035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms1035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053365240", 
          "https://doi.org/10.1038/ncomms1035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.bioconjchem.5b00064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055081927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.est.5b00637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055087488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jacs.5b01180", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055873804"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-10", 
    "datePublishedReg": "2016-10-01", 
    "description": "A novel label-free electrochemical strategy for nucleic acid detection was developed by using gold electrodes coated with mesoporous silica thin films as sensing interface. The biosensing approach relies on the covalent attachment of a capture DNA probe on the surface of the silica nanopores and further hybridization with its complementary target oligonucleotide sequence, causing a diffusion hindering of an Fe(CN)6 (3-/4-) electrochemical probe through the nanochannels of the mesoporous film. This DNA-mesoporous silica thin film-modified electrodes allowed sensitive (91.7\u00a0A/M) and rapid (45\u00a0min) detection of low nanomolar levels of synthetic target DNA (25\u00a0fmol) and were successfully employed to quantify the endogenous content of Escherichia coli 16S ribosomal RNA (rRNA) directly in raw bacterial lysate samples without isolation or purification steps. Moreover, the 1-month stability demonstrated by these biosensing devices enables their advanced preparation and storage, as desired for practical real-life applications. Graphical abstract Mesoporous silica thin films as scaffolds for the development of novel label-free electrochemical genosensors to perform selective, sensitive and rapid detection of target oligonucleotide sequences. Application towards E. coli determination. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00216-016-9608-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1357342", 
        "issn": [
          "1618-2642", 
          "1618-2650"
        ], 
        "name": "Analytical and Bioanalytical Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "26", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "408"
      }
    ], 
    "name": "Label-free electrochemical genosensor based on mesoporous silica thin film", 
    "pagination": "7321-7327", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bfea56ae2014e3abf0df2f3637df1b87ddb1c6d310fb6ed9d78408ec8f773753"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27236313"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101134327"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00216-016-9608-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1040288074"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00216-016-9608-7", 
      "https://app.dimensions.ai/details/publication/pub.1040288074"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00216-016-9608-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9608-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9608-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9608-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-016-9608-7'


 

This table displays all metadata directly associated to this object as RDF triples.

275 TRIPLES      21 PREDICATES      72 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00216-016-9608-7 schema:about N0d0eacbc917a4dc29c51b6a0f847f010
2 N114ed97ed5d940f799b2a200a6b652df
3 N2c1a0409fc8c42f49c4d081121696d6a
4 N5d3c6c1611d04f819484d2897c88abf2
5 N82a84b9aee2445a697956738250e2a55
6 N9a59cce840e34cd28bc04b91ce819094
7 Na007234dcebd4810b14d92bfc7d343f9
8 Nafd8d680c4734ea0b32b51915d1322f0
9 Nccb0d2f8fb7446a39d0973926232d8fd
10 Nd9838904a26147d780a0ac43afd5d0e1
11 Ne00893ec7d544b79aeabba18e04d8a10
12 Ne957505667594dc4b209260012e1d049
13 Nf223fc387e4743b9b32e3dc2ace508e4
14 Nfb81872e9cca467286ff4b8a17d2986e
15 anzsrc-for:03
16 anzsrc-for:0306
17 schema:author N9fe29a32e48241a19731ee665e7ac62e
18 schema:citation sg:pub.10.1007/s00604-014-1301-y
19 sg:pub.10.1007/s00604-015-1656-8
20 sg:pub.10.1007/s10008-014-2570-4
21 sg:pub.10.1038/ncomms1035
22 sg:pub.10.1038/nmat1951
23 https://doi.org/10.1002/cphc.201300045
24 https://doi.org/10.1016/j.ab.2014.04.028
25 https://doi.org/10.1016/j.ab.2014.09.023
26 https://doi.org/10.1016/j.bios.2013.05.008
27 https://doi.org/10.1016/j.bios.2014.07.078
28 https://doi.org/10.1016/j.bios.2015.05.034
29 https://doi.org/10.1016/j.elecom.2015.06.006
30 https://doi.org/10.1016/j.electacta.2013.12.019
31 https://doi.org/10.1016/j.electacta.2014.01.074
32 https://doi.org/10.1016/j.nantod.2014.04.002
33 https://doi.org/10.1016/j.snb.2014.10.043
34 https://doi.org/10.1016/j.snb.2015.08.005
35 https://doi.org/10.1016/j.talanta.2013.03.061
36 https://doi.org/10.1016/j.trac.2008.03.011
37 https://doi.org/10.1016/j.trac.2012.03.008
38 https://doi.org/10.1021/ac500618v
39 https://doi.org/10.1021/acs.bioconjchem.5b00064
40 https://doi.org/10.1021/acs.est.5b00637
41 https://doi.org/10.1021/cr500100j
42 https://doi.org/10.1021/jacs.5b01180
43 https://doi.org/10.1039/c3cs60141b
44 https://doi.org/10.1039/c4cc05083e
45 https://doi.org/10.1128/jcm.44.2.561-570.2006
46 https://doi.org/10.3109/07388551.2013.778228
47 schema:datePublished 2016-10
48 schema:datePublishedReg 2016-10-01
49 schema:description A novel label-free electrochemical strategy for nucleic acid detection was developed by using gold electrodes coated with mesoporous silica thin films as sensing interface. The biosensing approach relies on the covalent attachment of a capture DNA probe on the surface of the silica nanopores and further hybridization with its complementary target oligonucleotide sequence, causing a diffusion hindering of an Fe(CN)6 (3-/4-) electrochemical probe through the nanochannels of the mesoporous film. This DNA-mesoporous silica thin film-modified electrodes allowed sensitive (91.7 A/M) and rapid (45 min) detection of low nanomolar levels of synthetic target DNA (25 fmol) and were successfully employed to quantify the endogenous content of Escherichia coli 16S ribosomal RNA (rRNA) directly in raw bacterial lysate samples without isolation or purification steps. Moreover, the 1-month stability demonstrated by these biosensing devices enables their advanced preparation and storage, as desired for practical real-life applications. Graphical abstract Mesoporous silica thin films as scaffolds for the development of novel label-free electrochemical genosensors to perform selective, sensitive and rapid detection of target oligonucleotide sequences. Application towards E. coli determination.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf N1340d399595e405e8af26f2044910174
54 N40979a8c4f744b6e9d926998528f0203
55 sg:journal.1357342
56 schema:name Label-free electrochemical genosensor based on mesoporous silica thin film
57 schema:pagination 7321-7327
58 schema:productId N295ba4101e68485282a4cb2b5d0819c0
59 N3cb4491cb2e7472da4a1eff1f54d421c
60 Na7fdc17081d440e08e327913d6ab928c
61 Nc428df69e0b54d6f9db22f3c2fa9cfbd
62 Nf90575ff0e4c4e14a8c4b6d2b5620d1b
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040288074
64 https://doi.org/10.1007/s00216-016-9608-7
65 schema:sdDatePublished 2019-04-11T02:01
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher Nf49138d0db484a33be7e99ccf487223e
68 schema:url http://link.springer.com/10.1007%2Fs00216-016-9608-7
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N0d0eacbc917a4dc29c51b6a0f847f010 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name DNA
74 rdf:type schema:DefinedTerm
75 N114ed97ed5d940f799b2a200a6b652df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Escherichia coli Infections
77 rdf:type schema:DefinedTerm
78 N1340d399595e405e8af26f2044910174 schema:issueNumber 26
79 rdf:type schema:PublicationIssue
80 N295ba4101e68485282a4cb2b5d0819c0 schema:name readcube_id
81 schema:value bfea56ae2014e3abf0df2f3637df1b87ddb1c6d310fb6ed9d78408ec8f773753
82 rdf:type schema:PropertyValue
83 N2c1a0409fc8c42f49c4d081121696d6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Escherichia coli
85 rdf:type schema:DefinedTerm
86 N3cb4491cb2e7472da4a1eff1f54d421c schema:name doi
87 schema:value 10.1007/s00216-016-9608-7
88 rdf:type schema:PropertyValue
89 N40979a8c4f744b6e9d926998528f0203 schema:volumeNumber 408
90 rdf:type schema:PublicationVolume
91 N5d3c6c1611d04f819484d2897c88abf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Biosensing Techniques
93 rdf:type schema:DefinedTerm
94 N6ddb3ad478114336a0e1be5ab08c4396 rdf:first N813698bd96234e32a1ca20c24e73b7f2
95 rdf:rest Nd4e561cb287f49a0b66cba78a9b01ab5
96 N813698bd96234e32a1ca20c24e73b7f2 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
97 schema:familyName Luna
98 schema:givenName Gema
99 rdf:type schema:Person
100 N82a84b9aee2445a697956738250e2a55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Silicon Dioxide
102 rdf:type schema:DefinedTerm
103 N9a59cce840e34cd28bc04b91ce819094 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name RNA, Ribosomal
105 rdf:type schema:DefinedTerm
106 N9fe29a32e48241a19731ee665e7ac62e rdf:first sg:person.01313312101.17
107 rdf:rest Nb05d20d3fafe4098a556d03ac25ac949
108 Na007234dcebd4810b14d92bfc7d343f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Immobilized Nucleic Acids
110 rdf:type schema:DefinedTerm
111 Na78d9ffe360d4906adcf58a1e0ab9847 rdf:first sg:person.011213267641.19
112 rdf:rest Nc6b04463c18948ec9170529edfadf96c
113 Na7fdc17081d440e08e327913d6ab928c schema:name pubmed_id
114 schema:value 27236313
115 rdf:type schema:PropertyValue
116 Nafd8d680c4734ea0b32b51915d1322f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Equipment Design
118 rdf:type schema:DefinedTerm
119 Nb05d20d3fafe4098a556d03ac25ac949 rdf:first sg:person.01300744325.25
120 rdf:rest N6ddb3ad478114336a0e1be5ab08c4396
121 Nc428df69e0b54d6f9db22f3c2fa9cfbd schema:name dimensions_id
122 schema:value pub.1040288074
123 rdf:type schema:PropertyValue
124 Nc6b04463c18948ec9170529edfadf96c rdf:first sg:person.01224045767.93
125 rdf:rest Nfbf3d58cdede4818863ca223d2bee75c
126 Nc8fc7a5b902e44b58c06296a75d4aeb7 rdf:first sg:person.01037305730.48
127 rdf:rest Na78d9ffe360d4906adcf58a1e0ab9847
128 Nccb0d2f8fb7446a39d0973926232d8fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name RNA, Bacterial
130 rdf:type schema:DefinedTerm
131 Nd4e561cb287f49a0b66cba78a9b01ab5 rdf:first sg:person.015252373020.07
132 rdf:rest Nc8fc7a5b902e44b58c06296a75d4aeb7
133 Nd9838904a26147d780a0ac43afd5d0e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Humans
135 rdf:type schema:DefinedTerm
136 Nda6f2bd10e5d46cc815e51469bae2ac0 rdf:first sg:person.01217026335.99
137 rdf:rest rdf:nil
138 Ne00893ec7d544b79aeabba18e04d8a10 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Electrodes
140 rdf:type schema:DefinedTerm
141 Ne957505667594dc4b209260012e1d049 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Electrochemical Techniques
143 rdf:type schema:DefinedTerm
144 Nf223fc387e4743b9b32e3dc2ace508e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Nucleic Acid Hybridization
146 rdf:type schema:DefinedTerm
147 Nf49138d0db484a33be7e99ccf487223e schema:name Springer Nature - SN SciGraph project
148 rdf:type schema:Organization
149 Nf90575ff0e4c4e14a8c4b6d2b5620d1b schema:name nlm_unique_id
150 schema:value 101134327
151 rdf:type schema:PropertyValue
152 Nfb81872e9cca467286ff4b8a17d2986e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Porosity
154 rdf:type schema:DefinedTerm
155 Nfbf3d58cdede4818863ca223d2bee75c rdf:first sg:person.0627636725.35
156 rdf:rest Nda6f2bd10e5d46cc815e51469bae2ac0
157 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
158 schema:name Chemical Sciences
159 rdf:type schema:DefinedTerm
160 anzsrc-for:0306 schema:inDefinedTermSet anzsrc-for:
161 schema:name Physical Chemistry (incl. Structural)
162 rdf:type schema:DefinedTerm
163 sg:journal.1357342 schema:issn 1618-2642
164 1618-2650
165 schema:name Analytical and Bioanalytical Chemistry
166 rdf:type schema:Periodical
167 sg:person.01037305730.48 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
168 schema:familyName Campuzano
169 schema:givenName Susana
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037305730.48
171 rdf:type schema:Person
172 sg:person.011213267641.19 schema:affiliation https://www.grid.ac/institutes/grid.12574.35
173 schema:familyName Raouafi
174 schema:givenName Noureddine
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011213267641.19
176 rdf:type schema:Person
177 sg:person.01217026335.99 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
178 schema:familyName Villalonga
179 schema:givenName Reynaldo
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217026335.99
181 rdf:type schema:Person
182 sg:person.01224045767.93 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
183 schema:familyName Sánchez
184 schema:givenName Alfredo
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224045767.93
186 rdf:type schema:Person
187 sg:person.01300744325.25 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
188 schema:familyName Fernández
189 schema:givenName Iñigo
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300744325.25
191 rdf:type schema:Person
192 sg:person.01313312101.17 schema:affiliation https://www.grid.ac/institutes/grid.12574.35
193 schema:familyName Saadaoui
194 schema:givenName Maroua
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313312101.17
196 rdf:type schema:Person
197 sg:person.015252373020.07 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
198 schema:familyName Díez
199 schema:givenName Paula
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015252373020.07
201 rdf:type schema:Person
202 sg:person.0627636725.35 schema:affiliation https://www.grid.ac/institutes/grid.4795.f
203 schema:familyName Pingarrón
204 schema:givenName José M.
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627636725.35
206 rdf:type schema:Person
207 sg:pub.10.1007/s00604-014-1301-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1029175730
208 https://doi.org/10.1007/s00604-014-1301-y
209 rdf:type schema:CreativeWork
210 sg:pub.10.1007/s00604-015-1656-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030526804
211 https://doi.org/10.1007/s00604-015-1656-8
212 rdf:type schema:CreativeWork
213 sg:pub.10.1007/s10008-014-2570-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029461657
214 https://doi.org/10.1007/s10008-014-2570-4
215 rdf:type schema:CreativeWork
216 sg:pub.10.1038/ncomms1035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053365240
217 https://doi.org/10.1038/ncomms1035
218 rdf:type schema:CreativeWork
219 sg:pub.10.1038/nmat1951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051418404
220 https://doi.org/10.1038/nmat1951
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1002/cphc.201300045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013521922
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.ab.2014.04.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007046983
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.ab.2014.09.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039817760
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.bios.2013.05.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048279983
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.bios.2014.07.078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043609454
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.bios.2015.05.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019735569
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/j.elecom.2015.06.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013713125
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.electacta.2013.12.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044639982
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.electacta.2014.01.074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011272614
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.nantod.2014.04.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012294451
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.snb.2014.10.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001142944
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.snb.2015.08.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031775570
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.talanta.2013.03.061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005034686
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.trac.2008.03.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027182617
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.trac.2012.03.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034322486
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1021/ac500618v schema:sameAs https://app.dimensions.ai/details/publication/pub.1034492591
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1021/acs.bioconjchem.5b00064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055081927
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1021/acs.est.5b00637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055087488
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/cr500100j schema:sameAs https://app.dimensions.ai/details/publication/pub.1034632267
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1021/jacs.5b01180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055873804
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1039/c3cs60141b schema:sameAs https://app.dimensions.ai/details/publication/pub.1032216608
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1039/c4cc05083e schema:sameAs https://app.dimensions.ai/details/publication/pub.1037416453
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1128/jcm.44.2.561-570.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041554033
267 rdf:type schema:CreativeWork
268 https://doi.org/10.3109/07388551.2013.778228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035705785
269 rdf:type schema:CreativeWork
270 https://www.grid.ac/institutes/grid.12574.35 schema:alternateName Tunis El Manar University
271 schema:name Department of Chemistry, Faculty of Sciences, University of Tunis El Manar, Rue Béchir Salem Belkheria, Tunis El-Manar, 2092, Tunis, Tunisia
272 rdf:type schema:Organization
273 https://www.grid.ac/institutes/grid.4795.f schema:alternateName Complutense University of Madrid
274 schema:name Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain
275 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...