Revised sample preparation for the analysis of oxysterols by enzyme-assisted derivatisation for sterol analysis (EADSA) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-07

AUTHORS

Peter J. Crick, T. William Bentley, Yuqin Wang, William J. Griffiths

ABSTRACT

Sterols, and specifically oxysterols, play important roles in the biosynthesis of bile acids and steroid hormones as well as possessing biological activities in their own right. Analysis of oxysterols is complicated due to their low abundance in biological systems and poor ionisation characteristics in mass spectrometry. Over the past decade, we have developed a liquid chromatography-mass spectrometry method termed enzyme-assisted derivatisation for sterol analysis (EADSA). Our derivatisation procedure relies on two solid-phase extraction steps to (i) separate cholesterol from oxysterols and (ii) remove excess derivatisation reagents. Recent inter-batch variation in C18 reversed-phase cartridges has led us to experiment with alternative columns. Here, we present our findings and report an improved sample preparation procedure using polymeric hydrophilic-lipophilic balanced reversed-phase cartridges. More... »

PAGES

5235-5239

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-015-8609-2

DOI

http://dx.doi.org/10.1007/s00216-015-8609-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030386571

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25796527


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Analytical Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cholesterol", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cholesterol Oxidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Solid Phase Extraction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sterols", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Swiss Tropical and Public Health Institute", 
          "id": "https://www.grid.ac/institutes/grid.416786.a", 
          "name": [
            "College of Medicine, Swansea University, Grove Building, Singleton Park, SA2 8PP, Swansea, UK", 
            "Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crick", 
        "givenName": "Peter J.", 
        "id": "sg:person.01203677110.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203677110.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swansea University", 
          "id": "https://www.grid.ac/institutes/grid.4827.9", 
          "name": [
            "College of Medicine, Swansea University, Grove Building, Singleton Park, SA2 8PP, Swansea, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bentley", 
        "givenName": "T. William", 
        "id": "sg:person.0611046130.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611046130.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swansea University", 
          "id": "https://www.grid.ac/institutes/grid.4827.9", 
          "name": [
            "College of Medicine, Swansea University, Grove Building, Singleton Park, SA2 8PP, Swansea, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Yuqin", 
        "id": "sg:person.01155427064.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155427064.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swansea University", 
          "id": "https://www.grid.ac/institutes/grid.4827.9", 
          "name": [
            "College of Medicine, Swansea University, Grove Building, Singleton Park, SA2 8PP, Swansea, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Griffiths", 
        "givenName": "William J.", 
        "id": "sg:person.0746453563.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746453563.57"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.freeradbiomed.2012.07.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005385769"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.d022285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006169591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1995.1110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028419899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.steroids.2012.02.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030252183"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2013.01.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047636056"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-07", 
    "datePublishedReg": "2015-07-01", 
    "description": "Sterols, and specifically oxysterols, play important roles in the biosynthesis of bile acids and steroid hormones as well as possessing biological activities in their own right. Analysis of oxysterols is complicated due to their low abundance in biological systems and poor ionisation characteristics in mass spectrometry. Over the past decade, we have developed a liquid chromatography-mass spectrometry method termed enzyme-assisted derivatisation for sterol analysis (EADSA). Our derivatisation procedure relies on two solid-phase extraction steps to (i) separate cholesterol from oxysterols and (ii) remove excess derivatisation reagents. Recent inter-batch variation in C18 reversed-phase cartridges has led us to experiment with alternative columns. Here, we present our findings and report an improved sample preparation procedure using polymeric hydrophilic-lipophilic balanced reversed-phase cartridges. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00216-015-8609-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3560791", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2758330", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1357342", 
        "issn": [
          "1618-2642", 
          "1618-2650"
        ], 
        "name": "Analytical and Bioanalytical Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "17", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "407"
      }
    ], 
    "name": "Revised sample preparation for the analysis of oxysterols by enzyme-assisted derivatisation for sterol analysis (EADSA)", 
    "pagination": "5235-5239", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "90c1b8d7bdb2d6f442135f7dbcf92a6d8e48d160d4feda1e356719404508410c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25796527"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101134327"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00216-015-8609-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030386571"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00216-015-8609-2", 
      "https://app.dimensions.ai/details/publication/pub.1030386571"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89826_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s00216-015-8609-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00216-015-8609-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-015-8609-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-015-8609-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-015-8609-2'


 

This table displays all metadata directly associated to this object as RDF triples.

145 TRIPLES      21 PREDICATES      42 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00216-015-8609-2 schema:about N244f413ecff54a0b89a1ba9b0427cb55
2 N345c265d202a4261895e8a9d78a9a69a
3 N8dbeb4fc6f854dd5a5e853e62efec77d
4 Nab4e0f38133e462f90042a7baddb8db0
5 Nacb6bc265d484b65995371c891014956
6 Nb3b901ccfe9546d28a8bf1682d206eb7
7 Nc1eec07f07e243bd9df76ceedc8ef038
8 Ncf95b0736d5945478ea32563235ead21
9 anzsrc-for:03
10 anzsrc-for:0301
11 schema:author Nf03f69a2476c4d919d311123636ed862
12 schema:citation https://doi.org/10.1006/abio.1995.1110
13 https://doi.org/10.1016/j.bcp.2013.01.027
14 https://doi.org/10.1016/j.freeradbiomed.2012.07.027
15 https://doi.org/10.1016/j.steroids.2012.02.006
16 https://doi.org/10.1194/jlr.d022285
17 schema:datePublished 2015-07
18 schema:datePublishedReg 2015-07-01
19 schema:description Sterols, and specifically oxysterols, play important roles in the biosynthesis of bile acids and steroid hormones as well as possessing biological activities in their own right. Analysis of oxysterols is complicated due to their low abundance in biological systems and poor ionisation characteristics in mass spectrometry. Over the past decade, we have developed a liquid chromatography-mass spectrometry method termed enzyme-assisted derivatisation for sterol analysis (EADSA). Our derivatisation procedure relies on two solid-phase extraction steps to (i) separate cholesterol from oxysterols and (ii) remove excess derivatisation reagents. Recent inter-batch variation in C18 reversed-phase cartridges has led us to experiment with alternative columns. Here, we present our findings and report an improved sample preparation procedure using polymeric hydrophilic-lipophilic balanced reversed-phase cartridges.
20 schema:genre research_article
21 schema:inLanguage en
22 schema:isAccessibleForFree true
23 schema:isPartOf N8e278888b77e43d2bbcd88cde3539c06
24 Nf96f1d17a47645849309c7247aa84e18
25 sg:journal.1357342
26 schema:name Revised sample preparation for the analysis of oxysterols by enzyme-assisted derivatisation for sterol analysis (EADSA)
27 schema:pagination 5235-5239
28 schema:productId N30620fdb26704fb0bfb9e1a237f17619
29 N4c997123b4e9469aae0adc28dd7302cd
30 Nba12730d10e54a058e7da614c626e1fd
31 Nbad7194882cb42328965c4e005112b7e
32 Nbdea3d74ac6a48f485a08987a4c8fc26
33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030386571
34 https://doi.org/10.1007/s00216-015-8609-2
35 schema:sdDatePublished 2019-04-11T10:04
36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
37 schema:sdPublisher N9043497ad7ae4259abced0b6cb6a1f04
38 schema:url http://link.springer.com/10.1007/s00216-015-8609-2
39 sgo:license sg:explorer/license/
40 sgo:sdDataset articles
41 rdf:type schema:ScholarlyArticle
42 N244f413ecff54a0b89a1ba9b0427cb55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
43 schema:name Sterols
44 rdf:type schema:DefinedTerm
45 N30620fdb26704fb0bfb9e1a237f17619 schema:name dimensions_id
46 schema:value pub.1030386571
47 rdf:type schema:PropertyValue
48 N345c265d202a4261895e8a9d78a9a69a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
49 schema:name Solid Phase Extraction
50 rdf:type schema:DefinedTerm
51 N4c997123b4e9469aae0adc28dd7302cd schema:name doi
52 schema:value 10.1007/s00216-015-8609-2
53 rdf:type schema:PropertyValue
54 N8dbeb4fc6f854dd5a5e853e62efec77d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
55 schema:name Mass Spectrometry
56 rdf:type schema:DefinedTerm
57 N8e278888b77e43d2bbcd88cde3539c06 schema:issueNumber 17
58 rdf:type schema:PublicationIssue
59 N9043497ad7ae4259abced0b6cb6a1f04 schema:name Springer Nature - SN SciGraph project
60 rdf:type schema:Organization
61 Nab4e0f38133e462f90042a7baddb8db0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Cholesterol
63 rdf:type schema:DefinedTerm
64 Nacb6bc265d484b65995371c891014956 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Humans
66 rdf:type schema:DefinedTerm
67 Nb3b901ccfe9546d28a8bf1682d206eb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Cholesterol Oxidase
69 rdf:type schema:DefinedTerm
70 Nba12730d10e54a058e7da614c626e1fd schema:name nlm_unique_id
71 schema:value 101134327
72 rdf:type schema:PropertyValue
73 Nbad7194882cb42328965c4e005112b7e schema:name pubmed_id
74 schema:value 25796527
75 rdf:type schema:PropertyValue
76 Nbdea3d74ac6a48f485a08987a4c8fc26 schema:name readcube_id
77 schema:value 90c1b8d7bdb2d6f442135f7dbcf92a6d8e48d160d4feda1e356719404508410c
78 rdf:type schema:PropertyValue
79 Nc1eec07f07e243bd9df76ceedc8ef038 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Chromatography, Liquid
81 rdf:type schema:DefinedTerm
82 Ncf95b0736d5945478ea32563235ead21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Polymers
84 rdf:type schema:DefinedTerm
85 Nd5ea2baf6e3044f19fd7c895201c7a15 rdf:first sg:person.0746453563.57
86 rdf:rest rdf:nil
87 Nd8cacb2a4b0443798b29586bce6b20a0 rdf:first sg:person.01155427064.02
88 rdf:rest Nd5ea2baf6e3044f19fd7c895201c7a15
89 Ne8eb4876ebd74c698b68678dfa42507b rdf:first sg:person.0611046130.31
90 rdf:rest Nd8cacb2a4b0443798b29586bce6b20a0
91 Nf03f69a2476c4d919d311123636ed862 rdf:first sg:person.01203677110.29
92 rdf:rest Ne8eb4876ebd74c698b68678dfa42507b
93 Nf96f1d17a47645849309c7247aa84e18 schema:volumeNumber 407
94 rdf:type schema:PublicationVolume
95 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
96 schema:name Chemical Sciences
97 rdf:type schema:DefinedTerm
98 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
99 schema:name Analytical Chemistry
100 rdf:type schema:DefinedTerm
101 sg:grant.2758330 http://pending.schema.org/fundedItem sg:pub.10.1007/s00216-015-8609-2
102 rdf:type schema:MonetaryGrant
103 sg:grant.3560791 http://pending.schema.org/fundedItem sg:pub.10.1007/s00216-015-8609-2
104 rdf:type schema:MonetaryGrant
105 sg:journal.1357342 schema:issn 1618-2642
106 1618-2650
107 schema:name Analytical and Bioanalytical Chemistry
108 rdf:type schema:Periodical
109 sg:person.01155427064.02 schema:affiliation https://www.grid.ac/institutes/grid.4827.9
110 schema:familyName Wang
111 schema:givenName Yuqin
112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155427064.02
113 rdf:type schema:Person
114 sg:person.01203677110.29 schema:affiliation https://www.grid.ac/institutes/grid.416786.a
115 schema:familyName Crick
116 schema:givenName Peter J.
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203677110.29
118 rdf:type schema:Person
119 sg:person.0611046130.31 schema:affiliation https://www.grid.ac/institutes/grid.4827.9
120 schema:familyName Bentley
121 schema:givenName T. William
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611046130.31
123 rdf:type schema:Person
124 sg:person.0746453563.57 schema:affiliation https://www.grid.ac/institutes/grid.4827.9
125 schema:familyName Griffiths
126 schema:givenName William J.
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746453563.57
128 rdf:type schema:Person
129 https://doi.org/10.1006/abio.1995.1110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028419899
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1016/j.bcp.2013.01.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047636056
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1016/j.freeradbiomed.2012.07.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005385769
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1016/j.steroids.2012.02.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030252183
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1194/jlr.d022285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006169591
138 rdf:type schema:CreativeWork
139 https://www.grid.ac/institutes/grid.416786.a schema:alternateName Swiss Tropical and Public Health Institute
140 schema:name College of Medicine, Swansea University, Grove Building, Singleton Park, SA2 8PP, Swansea, UK
141 Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland
142 rdf:type schema:Organization
143 https://www.grid.ac/institutes/grid.4827.9 schema:alternateName Swansea University
144 schema:name College of Medicine, Swansea University, Grove Building, Singleton Park, SA2 8PP, Swansea, UK
145 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...