A new ELISA for the quantification of equine procalcitonin in plasma as potential inflammation biomarker in horses View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-09

AUTHORS

Martin Rieger, Christian Kochleus, Dana Teschner, Daniela Rascher, Ann Kristin Barton, Arie Geerlof, Elisabeth Kremmer, Michael Schmid, Anton Hartmann, Heidrun Gehlen

ABSTRACT

In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISA was characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL(-1) was defined as within this range; both intra- and interassay variances were below 15 %. The target recovery ranged between 73 and 106 %. The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P = 0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL(-1)). More... »

PAGES

5507-5512

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-014-7944-z

DOI

http://dx.doi.org/10.1007/s00216-014-7944-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024661874

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24928115


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