Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection View Full Text


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Article Info

DATE

2014-05

AUTHORS

Qiuling Zhao, Zhen Zhang, Li Xu, Ted Xia, Nan Li, Jianli Liu, Xiaohong Fang

ABSTRACT

A novel enzyme-linked aptamer assay (ELAA) with the aid of Exonuclease I (Exo I) for colorimetric detection of small molecules was developed. The fluorescein isothiocyanate (FITC)-labeled aptamer was integrated into a double-stranded DNA (dsDNA). In the presence of target, the binding of aptamer with target protected the aptamer from Exo I degradation, which resulted in the FITC tag remaining on the aptamer. Then, the anti-FITC-HRP conjugate was used to produce an optically observable signal. By monitoring the color change, we were able to detect two model molecules, ATP and L-argininamide, with high selectivity and high sensitivity even in the serum matrix. It is expected to be a simple and general ELAA method with wide applicability. More... »

PAGES

2949-2955

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-014-7705-z

DOI

http://dx.doi.org/10.1007/s00216-014-7705-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029194705

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24599422


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curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-014-7705-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-014-7705-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-014-7705-z'


 

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