On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-03

AUTHORS

Christoph A. Wehe, Imke Pieper, Michael Holtkamp, Georgina M. Thyssen, Michael Sperling, Tanja Schwerdtle, Uwe Karst

ABSTRACT

In order to reveal the time-depending mercury species uptake by human astrocytes, a novel approach for total mercury analysis is presented, which uses an accelerated sample introduction system combined on-line with an inductively coupled plasma mass spectrometer equipped with a collision/reaction cell. Human astrocyte samples were incubated with inorganic mercury (HgCl2), methylmercury chloride (MeHgCl), and thimerosal. After 1-h incubation with Hg(2+), cellular concentrations of 3 μM were obtained, whereas for organic species, concentrations of 14-18 μM could be found. After 24 h, a cellular accumulation factor of 0.3 was observed for the cells incubated with Hg(2+), whereas the organic species both showed values of about 5. Due to the obtained steady-state signals, reliable results with relative standard deviations of well below 5 % and limits of detection in the concentration range of 1 ng L(-1) were obtained using external calibration and species-unspecific isotope dilution analysis approaches. The results were further validated using atomic fluorescence spectrometry. More... »

PAGES

1909-1916

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-013-7608-4

DOI

http://dx.doi.org/10.1007/s00216-013-7608-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033549036

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24442014


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