Identification of proteins interacting with ammodytoxins in Vipera ammodytes ammodytes venom by immuno-affinity chromatography View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-01

AUTHORS

Marija Brgles, Tihana Kurtović, Lidija Kovačič, Igor Križaj, Miloš Barut, Maja Lang Balija, Günter Allmaier, Martina Marchetti-Deschmann, Beata Halassy

ABSTRACT

In order to perform their function, proteins frequently interact with other proteins. Various methods are used to reveal protein interacting partners, and affinity chromatography is one of them. Snake venom is composed mostly of proteins, and various protein complexes in the venom have been found to exhibit higher toxicity levels than respective components separately. Complexes can modulate envenomation activity of a venom and/or potentiate its effect. Our previous data indicate that the most toxic components of the Vipera ammodytes ammodytes (Vaa) venom isolated so far-ammodytoxins (Atxs)-are contributing to the venom's toxicity only moderately; therefore, we aimed to explore whether they have some interacting partner(s) potentiating toxicity. For screening of possible interactions, immuno-affinity chromatography combined with identification by mass spectrometry was used. Various chemistries (epoxy, carbonyldiimidazole, ethylenediamine) as well as protein G functionality were used to immobilize antibodies on monolith support, a Convective Interaction Media disk. Monoliths have been demonstrated to better suit the separation of large biomolecules. Using such approach, several proteins were indicated as potential Atx-binding proteins. Among these, the interaction of Atxs with a Kunitz-type inhibitor was confirmed by far-Western dot-blot and surface plasmon resonance measurement. It can be concluded that affinity chromatography on monolithic columns combined with mass spectrometry identification is a successful approach for screening of protein interactions and it resulted with detection of the interaction of Atx with Kunitz-type inhibitor in Vaa venom for the first time. More... »

PAGES

293-304

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-013-7453-5

DOI

http://dx.doi.org/10.1007/s00216-013-7453-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046504211

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24217948


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Immobilized", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Affinity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Interaction Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reptilian Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viper Venoms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viperidae", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Zagreb", 
          "id": "https://www.grid.ac/institutes/grid.4808.4", 
          "name": [
            "Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Rockefellerova 10, 10000, Zagreb, Croatia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brgles", 
        "givenName": "Marija", 
        "id": "sg:person.0670756651.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670756651.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Zagreb", 
          "id": "https://www.grid.ac/institutes/grid.4808.4", 
          "name": [
            "Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Rockefellerova 10, 10000, Zagreb, Croatia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kurtovi\u0107", 
        "givenName": "Tihana", 
        "id": "sg:person.0705715073.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705715073.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jo\u017eef Stefan Institute", 
          "id": "https://www.grid.ac/institutes/grid.11375.31", 
          "name": [
            "Department of Molecular and Biomedical Sciences, Jo\u017eef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kova\u010di\u010d", 
        "givenName": "Lidija", 
        "id": "sg:person.01313635223.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313635223.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins", 
          "id": "https://www.grid.ac/institutes/grid.457168.9", 
          "name": [
            "Department of Molecular and Biomedical Sciences, Jo\u017eef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia", 
            "Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, A\u0161ker\u010deva 5, 1000, Ljubljana, Slovenia", 
            "Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000, Ljubljana, Slovenia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kri\u017eaj", 
        "givenName": "Igor", 
        "id": "sg:person.01062475024.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062475024.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BIA Separations (Slovenia)", 
          "id": "https://www.grid.ac/institutes/grid.432846.f", 
          "name": [
            "BIA Separations, Mirce 21, 5270, Ajdov\u0161\u010dina, Slovenia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Barut", 
        "givenName": "Milo\u0161", 
        "id": "sg:person.0626103532.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626103532.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Immunology", 
          "id": "https://www.grid.ac/institutes/grid.418761.d", 
          "name": [
            "Institute of Immunology, Rockefellerova 2, 10000, Zagreb, Croatia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lang Balija", 
        "givenName": "Maja", 
        "id": "sg:person.01053131150.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053131150.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "TU Wien", 
          "id": "https://www.grid.ac/institutes/grid.5329.d", 
          "name": [
            "Institute of Chemical Technologies and Analytics, Vienna University of Technology, 1060, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Allmaier", 
        "givenName": "G\u00fcnter", 
        "id": "sg:person.0617034703.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617034703.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "TU Wien", 
          "id": "https://www.grid.ac/institutes/grid.5329.d", 
          "name": [
            "Institute of Chemical Technologies and Analytics, Vienna University of Technology, 1060, Vienna, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Marchetti-Deschmann", 
        "givenName": "Martina", 
        "id": "sg:person.0755674003.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755674003.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Zagreb", 
          "id": "https://www.grid.ac/institutes/grid.4808.4", 
          "name": [
            "Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Rockefellerova 10, 10000, Zagreb, Croatia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Halassy", 
        "givenName": "Beata", 
        "id": "sg:person.0736702550.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736702550.37"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0014-5793(03)01357-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001637916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2012.03.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001643001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jchromb.2006.10.056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002225674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac100818g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002518005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac100818g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002518005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pcbi.1000807", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003546024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10607", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004196516", 
          "https://doi.org/10.1038/nature10607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(03)00693-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008260289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cca.2005.02.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009134784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.toxicon.2011.06.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011797100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jssc.200401800", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012795205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1976.tb10833.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013211288"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c100048200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015008107"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016255758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016255758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2003.08.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017456316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jpba.2007.12.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019197832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(78)90058-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019853372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(78)90058-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019853372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/479306a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020775903", 
          "https://doi.org/10.1038/479306a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(83)90057-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021240276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0041-0101(83)90057-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021240276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2008.05.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021297335"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chroma.2007.12.087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022409415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.1999.0857", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025520750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.1999.0857", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025520750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2008.06.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027114114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.1.13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031587796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2006.06.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034847558"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3030171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037621909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3030171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037621909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-009-0050-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037669605", 
          "https://doi.org/10.1007/s00018-009-0050-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-009-0050-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037669605", 
          "https://doi.org/10.1007/s00018-009-0050-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-009-0050-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037669605", 
          "https://doi.org/10.1007/s00018-009-0050-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.1.7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037751554"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0165-022x(01)00211-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039881842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pep.2010.04.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040834334"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biophys.29.1.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040990908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2009.10.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041654246"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cbpc.2008.05.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041672235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2008.06.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043827507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0395-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044906782", 
          "https://doi.org/10.1007/s00253-006-0395-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0395-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044906782", 
          "https://doi.org/10.1007/s00253-006-0395-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(73)90374-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045391691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045828427", 
          "https://doi.org/10.1038/13732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045828427", 
          "https://doi.org/10.1038/13732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-012-5754-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046155832", 
          "https://doi.org/10.1007/s00216-012-5754-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(03)00228-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048731374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/10_2007_090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051055432", 
          "https://doi.org/10.1007/10_2007_090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/10_2007_090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051055432", 
          "https://doi.org/10.1007/10_2007_090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/pr201211w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051860209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00346a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055171246"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/pr070376c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056291691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081856294", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-01", 
    "datePublishedReg": "2014-01-01", 
    "description": "In order to perform their function, proteins frequently interact with other proteins. Various methods are used to reveal protein interacting partners, and affinity chromatography is one of them. Snake venom is composed mostly of proteins, and various protein complexes in the venom have been found to exhibit higher toxicity levels than respective components separately. Complexes can modulate envenomation activity of a venom and/or potentiate its effect. Our previous data indicate that the most toxic components of the Vipera ammodytes ammodytes (Vaa) venom isolated so far-ammodytoxins (Atxs)-are contributing to the venom's toxicity only moderately; therefore, we aimed to explore whether they have some interacting partner(s) potentiating toxicity. For screening of possible interactions, immuno-affinity chromatography combined with identification by mass spectrometry was used. Various chemistries (epoxy, carbonyldiimidazole, ethylenediamine) as well as protein G functionality were used to immobilize antibodies on monolith support, a Convective Interaction Media disk. Monoliths have been demonstrated to better suit the separation of large biomolecules. Using such approach, several proteins were indicated as potential Atx-binding proteins. Among these, the interaction of Atxs with a Kunitz-type inhibitor was confirmed by far-Western dot-blot and surface plasmon resonance measurement. It can be concluded that affinity chromatography on monolithic columns combined with mass spectrometry identification is a successful approach for screening of protein interactions and it resulted with detection of the interaction of Atx with Kunitz-type inhibitor in Vaa venom for the first time. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00216-013-7453-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1357342", 
        "issn": [
          "1618-2642", 
          "1618-2650"
        ], 
        "name": "Analytical and Bioanalytical Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "406"
      }
    ], 
    "name": "Identification of proteins interacting with ammodytoxins in Vipera ammodytes ammodytes venom by immuno-affinity chromatography", 
    "pagination": "293-304", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "dd872046ed1e9f3182031965027d4c7cef556f07a1fdc57c0e09934327751b0d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24217948"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101134327"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00216-013-7453-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046504211"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00216-013-7453-5", 
      "https://app.dimensions.ai/details/publication/pub.1046504211"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00216-013-7453-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00216-013-7453-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-013-7453-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-013-7453-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-013-7453-5'


 

This table displays all metadata directly associated to this object as RDF triples.

321 TRIPLES      21 PREDICATES      83 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00216-013-7453-5 schema:about N0799152ad15542aaa982f91ca813ee1f
2 N3d82635b47cf4fc2a7a6eb1059cc4930
3 N604b239536f448108a29574efc526b3b
4 N62bc014c932d4ccb8ec5b392a996b30a
5 N678ed63d8f69474ba826cf26ae17293c
6 N80a7a86f5c0046ea8e4361ac580ddbe5
7 N85e05468e55e4610a4a24855e360ed75
8 Naeef21ffa8f542feb3493fe2f0ff9c9a
9 Ncc0f9d5e22f84ed1b6e07446ed6f7fe4
10 Nd0a711990eae4b2097acfe3dad047424
11 Ne0b5e069153b491b803fd71ea7588444
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author N54186919d685465e94fafd2dc5cf80da
15 schema:citation sg:pub.10.1007/10_2007_090
16 sg:pub.10.1007/s00018-009-0050-2
17 sg:pub.10.1007/s00216-012-5754-8
18 sg:pub.10.1007/s00253-006-0395-5
19 sg:pub.10.1038/13732
20 sg:pub.10.1038/479306a
21 sg:pub.10.1038/nature10607
22 https://app.dimensions.ai/details/publication/pub.1081856294
23 https://doi.org/10.1002/jssc.200401800
24 https://doi.org/10.1006/meth.1999.0857
25 https://doi.org/10.1006/meth.2001.1183
26 https://doi.org/10.1016/0003-2697(73)90374-6
27 https://doi.org/10.1016/0041-0101(78)90058-2
28 https://doi.org/10.1016/0041-0101(83)90057-0
29 https://doi.org/10.1016/j.ab.2003.08.031
30 https://doi.org/10.1016/j.biochi.2006.06.008
31 https://doi.org/10.1016/j.biochi.2008.05.018
32 https://doi.org/10.1016/j.cbpc.2008.05.005
33 https://doi.org/10.1016/j.cca.2005.02.008
34 https://doi.org/10.1016/j.chroma.2007.12.087
35 https://doi.org/10.1016/j.copbio.2008.06.002
36 https://doi.org/10.1016/j.copbio.2008.06.005
37 https://doi.org/10.1016/j.jchromb.2006.10.056
38 https://doi.org/10.1016/j.jpba.2007.12.022
39 https://doi.org/10.1016/j.jsb.2009.10.010
40 https://doi.org/10.1016/j.pep.2010.04.009
41 https://doi.org/10.1016/j.toxicon.2011.06.009
42 https://doi.org/10.1016/j.toxicon.2012.03.009
43 https://doi.org/10.1016/s0006-291x(03)00228-6
44 https://doi.org/10.1016/s0014-5793(03)00693-8
45 https://doi.org/10.1016/s0014-5793(03)01357-7
46 https://doi.org/10.1016/s0165-022x(01)00211-1
47 https://doi.org/10.1021/ac100818g
48 https://doi.org/10.1021/bi00346a005
49 https://doi.org/10.1021/pr070376c
50 https://doi.org/10.1021/pr201211w
51 https://doi.org/10.1042/bj3030171
52 https://doi.org/10.1073/pnas.93.1.13
53 https://doi.org/10.1073/pnas.93.1.7
54 https://doi.org/10.1074/jbc.c100048200
55 https://doi.org/10.1111/j.1432-1033.1976.tb10833.x
56 https://doi.org/10.1146/annurev.biophys.29.1.1
57 https://doi.org/10.1371/journal.pcbi.1000807
58 schema:datePublished 2014-01
59 schema:datePublishedReg 2014-01-01
60 schema:description In order to perform their function, proteins frequently interact with other proteins. Various methods are used to reveal protein interacting partners, and affinity chromatography is one of them. Snake venom is composed mostly of proteins, and various protein complexes in the venom have been found to exhibit higher toxicity levels than respective components separately. Complexes can modulate envenomation activity of a venom and/or potentiate its effect. Our previous data indicate that the most toxic components of the Vipera ammodytes ammodytes (Vaa) venom isolated so far-ammodytoxins (Atxs)-are contributing to the venom's toxicity only moderately; therefore, we aimed to explore whether they have some interacting partner(s) potentiating toxicity. For screening of possible interactions, immuno-affinity chromatography combined with identification by mass spectrometry was used. Various chemistries (epoxy, carbonyldiimidazole, ethylenediamine) as well as protein G functionality were used to immobilize antibodies on monolith support, a Convective Interaction Media disk. Monoliths have been demonstrated to better suit the separation of large biomolecules. Using such approach, several proteins were indicated as potential Atx-binding proteins. Among these, the interaction of Atxs with a Kunitz-type inhibitor was confirmed by far-Western dot-blot and surface plasmon resonance measurement. It can be concluded that affinity chromatography on monolithic columns combined with mass spectrometry identification is a successful approach for screening of protein interactions and it resulted with detection of the interaction of Atx with Kunitz-type inhibitor in Vaa venom for the first time.
61 schema:genre research_article
62 schema:inLanguage en
63 schema:isAccessibleForFree false
64 schema:isPartOf N589dc7a4b7164f13ae4078f5acfb3f85
65 Nce248a67b0d54701bbe319a19813b473
66 sg:journal.1357342
67 schema:name Identification of proteins interacting with ammodytoxins in Vipera ammodytes ammodytes venom by immuno-affinity chromatography
68 schema:pagination 293-304
69 schema:productId N37bd41c8d8f44ca09f12761a98f7df20
70 N4deb27ed841542fba5fa17b03ee77112
71 N78e23c9c269242a9a5c9e99562237450
72 Na915356b59b24c3a97d38c0dae21dc86
73 Nbb9816b51dce4a1e9a7535de556be812
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046504211
75 https://doi.org/10.1007/s00216-013-7453-5
76 schema:sdDatePublished 2019-04-10T17:32
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Nc3097e42d6de4fd1839cdb70a187833b
79 schema:url http://link.springer.com/10.1007%2Fs00216-013-7453-5
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N0799152ad15542aaa982f91ca813ee1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Animals
85 rdf:type schema:DefinedTerm
86 N0d583cea69ad4e2882e4dcf04a868a8b rdf:first sg:person.0755674003.83
87 rdf:rest Ncb3065f81ea64906a1e6eeecb33c5424
88 N0fdd1b425b0c4e1c827f88762ca07f95 rdf:first sg:person.0705715073.26
89 rdf:rest Nfc8f7956c9954df8bf573e4ce1d2a5ea
90 N2d6a9754e27e4925a5baea48948a47b3 rdf:first sg:person.0626103532.67
91 rdf:rest N63a3a70aa9b140e88e3c2078edc8d521
92 N37bd41c8d8f44ca09f12761a98f7df20 schema:name pubmed_id
93 schema:value 24217948
94 rdf:type schema:PropertyValue
95 N3d82635b47cf4fc2a7a6eb1059cc4930 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Peptides
97 rdf:type schema:DefinedTerm
98 N4deb27ed841542fba5fa17b03ee77112 schema:name doi
99 schema:value 10.1007/s00216-013-7453-5
100 rdf:type schema:PropertyValue
101 N4e2a1f1c79bf40c39cdfad73b3a8fed3 rdf:first sg:person.0617034703.42
102 rdf:rest N0d583cea69ad4e2882e4dcf04a868a8b
103 N54186919d685465e94fafd2dc5cf80da rdf:first sg:person.0670756651.87
104 rdf:rest N0fdd1b425b0c4e1c827f88762ca07f95
105 N589dc7a4b7164f13ae4078f5acfb3f85 schema:volumeNumber 406
106 rdf:type schema:PublicationVolume
107 N604b239536f448108a29574efc526b3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Chromatography, Affinity
109 rdf:type schema:DefinedTerm
110 N62bc014c932d4ccb8ec5b392a996b30a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Protein Binding
112 rdf:type schema:DefinedTerm
113 N63a3a70aa9b140e88e3c2078edc8d521 rdf:first sg:person.01053131150.00
114 rdf:rest N4e2a1f1c79bf40c39cdfad73b3a8fed3
115 N678ed63d8f69474ba826cf26ae17293c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Viper Venoms
117 rdf:type schema:DefinedTerm
118 N78e23c9c269242a9a5c9e99562237450 schema:name nlm_unique_id
119 schema:value 101134327
120 rdf:type schema:PropertyValue
121 N80a7a86f5c0046ea8e4361ac580ddbe5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Antibodies, Immobilized
123 rdf:type schema:DefinedTerm
124 N85e05468e55e4610a4a24855e360ed75 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Mass Spectrometry
126 rdf:type schema:DefinedTerm
127 Na915356b59b24c3a97d38c0dae21dc86 schema:name readcube_id
128 schema:value dd872046ed1e9f3182031965027d4c7cef556f07a1fdc57c0e09934327751b0d
129 rdf:type schema:PropertyValue
130 Naeef21ffa8f542feb3493fe2f0ff9c9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Reptilian Proteins
132 rdf:type schema:DefinedTerm
133 Nbb9816b51dce4a1e9a7535de556be812 schema:name dimensions_id
134 schema:value pub.1046504211
135 rdf:type schema:PropertyValue
136 Nbcf5fb334a324c1986be182ca7a6d836 rdf:first sg:person.01062475024.75
137 rdf:rest N2d6a9754e27e4925a5baea48948a47b3
138 Nc3097e42d6de4fd1839cdb70a187833b schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 Ncb3065f81ea64906a1e6eeecb33c5424 rdf:first sg:person.0736702550.37
141 rdf:rest rdf:nil
142 Ncc0f9d5e22f84ed1b6e07446ed6f7fe4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Protein Interaction Mapping
144 rdf:type schema:DefinedTerm
145 Nce248a67b0d54701bbe319a19813b473 schema:issueNumber 1
146 rdf:type schema:PublicationIssue
147 Nd0a711990eae4b2097acfe3dad047424 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Plant Proteins
149 rdf:type schema:DefinedTerm
150 Ne0b5e069153b491b803fd71ea7588444 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Viperidae
152 rdf:type schema:DefinedTerm
153 Nfc8f7956c9954df8bf573e4ce1d2a5ea rdf:first sg:person.01313635223.25
154 rdf:rest Nbcf5fb334a324c1986be182ca7a6d836
155 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
156 schema:name Biological Sciences
157 rdf:type schema:DefinedTerm
158 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
159 schema:name Biochemistry and Cell Biology
160 rdf:type schema:DefinedTerm
161 sg:journal.1357342 schema:issn 1618-2642
162 1618-2650
163 schema:name Analytical and Bioanalytical Chemistry
164 rdf:type schema:Periodical
165 sg:person.01053131150.00 schema:affiliation https://www.grid.ac/institutes/grid.418761.d
166 schema:familyName Lang Balija
167 schema:givenName Maja
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053131150.00
169 rdf:type schema:Person
170 sg:person.01062475024.75 schema:affiliation https://www.grid.ac/institutes/grid.457168.9
171 schema:familyName Križaj
172 schema:givenName Igor
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062475024.75
174 rdf:type schema:Person
175 sg:person.01313635223.25 schema:affiliation https://www.grid.ac/institutes/grid.11375.31
176 schema:familyName Kovačič
177 schema:givenName Lidija
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313635223.25
179 rdf:type schema:Person
180 sg:person.0617034703.42 schema:affiliation https://www.grid.ac/institutes/grid.5329.d
181 schema:familyName Allmaier
182 schema:givenName Günter
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617034703.42
184 rdf:type schema:Person
185 sg:person.0626103532.67 schema:affiliation https://www.grid.ac/institutes/grid.432846.f
186 schema:familyName Barut
187 schema:givenName Miloš
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626103532.67
189 rdf:type schema:Person
190 sg:person.0670756651.87 schema:affiliation https://www.grid.ac/institutes/grid.4808.4
191 schema:familyName Brgles
192 schema:givenName Marija
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670756651.87
194 rdf:type schema:Person
195 sg:person.0705715073.26 schema:affiliation https://www.grid.ac/institutes/grid.4808.4
196 schema:familyName Kurtović
197 schema:givenName Tihana
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705715073.26
199 rdf:type schema:Person
200 sg:person.0736702550.37 schema:affiliation https://www.grid.ac/institutes/grid.4808.4
201 schema:familyName Halassy
202 schema:givenName Beata
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736702550.37
204 rdf:type schema:Person
205 sg:person.0755674003.83 schema:affiliation https://www.grid.ac/institutes/grid.5329.d
206 schema:familyName Marchetti-Deschmann
207 schema:givenName Martina
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755674003.83
209 rdf:type schema:Person
210 sg:pub.10.1007/10_2007_090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051055432
211 https://doi.org/10.1007/10_2007_090
212 rdf:type schema:CreativeWork
213 sg:pub.10.1007/s00018-009-0050-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037669605
214 https://doi.org/10.1007/s00018-009-0050-2
215 rdf:type schema:CreativeWork
216 sg:pub.10.1007/s00216-012-5754-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046155832
217 https://doi.org/10.1007/s00216-012-5754-8
218 rdf:type schema:CreativeWork
219 sg:pub.10.1007/s00253-006-0395-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044906782
220 https://doi.org/10.1007/s00253-006-0395-5
221 rdf:type schema:CreativeWork
222 sg:pub.10.1038/13732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045828427
223 https://doi.org/10.1038/13732
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/479306a schema:sameAs https://app.dimensions.ai/details/publication/pub.1020775903
226 https://doi.org/10.1038/479306a
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/nature10607 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004196516
229 https://doi.org/10.1038/nature10607
230 rdf:type schema:CreativeWork
231 https://app.dimensions.ai/details/publication/pub.1081856294 schema:CreativeWork
232 https://doi.org/10.1002/jssc.200401800 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012795205
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1006/meth.1999.0857 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025520750
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1006/meth.2001.1183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016255758
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0003-2697(73)90374-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045391691
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/0041-0101(78)90058-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019853372
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/0041-0101(83)90057-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021240276
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.ab.2003.08.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017456316
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.biochi.2006.06.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034847558
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.biochi.2008.05.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021297335
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.cbpc.2008.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041672235
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/j.cca.2005.02.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009134784
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/j.chroma.2007.12.087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022409415
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/j.copbio.2008.06.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043827507
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/j.copbio.2008.06.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027114114
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/j.jchromb.2006.10.056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002225674
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1016/j.jpba.2007.12.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019197832
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1016/j.jsb.2009.10.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041654246
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/j.pep.2010.04.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040834334
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/j.toxicon.2011.06.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011797100
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/j.toxicon.2012.03.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001643001
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/s0006-291x(03)00228-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048731374
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/s0014-5793(03)00693-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008260289
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/s0014-5793(03)01357-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001637916
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/s0165-022x(01)00211-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039881842
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1021/ac100818g schema:sameAs https://app.dimensions.ai/details/publication/pub.1002518005
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1021/bi00346a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055171246
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1021/pr070376c schema:sameAs https://app.dimensions.ai/details/publication/pub.1056291691
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1021/pr201211w schema:sameAs https://app.dimensions.ai/details/publication/pub.1051860209
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1042/bj3030171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037621909
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1073/pnas.93.1.13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031587796
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1073/pnas.93.1.7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037751554
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1074/jbc.c100048200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015008107
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1111/j.1432-1033.1976.tb10833.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013211288
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1146/annurev.biophys.29.1.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040990908
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1371/journal.pcbi.1000807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003546024
301 rdf:type schema:CreativeWork
302 https://www.grid.ac/institutes/grid.11375.31 schema:alternateName Jožef Stefan Institute
303 schema:name Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia
304 rdf:type schema:Organization
305 https://www.grid.ac/institutes/grid.418761.d schema:alternateName Institute of Immunology
306 schema:name Institute of Immunology, Rockefellerova 2, 10000, Zagreb, Croatia
307 rdf:type schema:Organization
308 https://www.grid.ac/institutes/grid.432846.f schema:alternateName BIA Separations (Slovenia)
309 schema:name BIA Separations, Mirce 21, 5270, Ajdovščina, Slovenia
310 rdf:type schema:Organization
311 https://www.grid.ac/institutes/grid.457168.9 schema:alternateName Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins
312 schema:name Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000, Ljubljana, Slovenia
313 Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000, Ljubljana, Slovenia
314 Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia
315 rdf:type schema:Organization
316 https://www.grid.ac/institutes/grid.4808.4 schema:alternateName University of Zagreb
317 schema:name Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Rockefellerova 10, 10000, Zagreb, Croatia
318 rdf:type schema:Organization
319 https://www.grid.ac/institutes/grid.5329.d schema:alternateName TU Wien
320 schema:name Institute of Chemical Technologies and Analytics, Vienna University of Technology, 1060, Vienna, Austria
321 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...