An insight into iTRAQ: where do we stand now? View Full Text


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Article Info

DATE

2012-03-27

AUTHORS

Caroline Evans, Josselin Noirel, Saw Yen Ow, Malinda Salim, Ana G. Pereira-Medrano, Narciso Couto, Jagroop Pandhal, Duncan Smith, Trong Khoa Pham, Esther Karunakaran, Xin Zou, Catherine A. Biggs, Phillip C. Wright

ABSTRACT

The iTRAQ (isobaric tags for relative and absolute quantification) technique is widely employed in proteomic workflows requiring relative quantification. Here, we review the iTRAQ literature; in particular, we focus on iTRAQ usage in relation to other commonly used quantitative techniques e.g. stable isotope labelling in culture (SILAC), label-free methods and selected reaction monitoring (SRM). As a result, we identify several issues arising with respect to iTRAQ. Perhaps frustratingly, iTRAQ’s attractiveness has been undermined by a number of technical and analytical limitations: it may not be truly quantitative, as the changes in abundance reported will generally be underestimated. We discuss weaknesses and strengths of iTRAQ as a methodology for relative quantification in the light of this and other technical issues. We focus on technical developments targeted at iTRAQ accuracy and precision, use of 4-plex over 8-plex reagents and application of iTRAQ to post-translational modification (PTM) workflows. We also discuss iTRAQ in relation to label-free approaches, to which iTRAQ is losing ground.Figure More... »

PAGES

1011-1027

References to SciGraph publications

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    http://scigraph.springernature.com/pub.10.1007/s00216-012-5918-6

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    http://dx.doi.org/10.1007/s00216-012-5918-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003801812

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22451173


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