Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development View Full Text


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Article Info

DATE

2012-02-02

AUTHORS

Carlos Briones, Miguel Moreno

ABSTRACT

Nucleic acid biosensors have a growing number of applications in genetics and biomedicine. This contribution is a critical review of the current state of the art concerning the use of nucleic acid analogues, in particular peptide nucleic acids (PNA) and locked nucleic acids (LNA), for the development of high-performance affinity biosensors. Both PNA and LNA have outstanding affinity for natural nucleic acids, and the destabilizing effect of base mismatches in PNA- or LNA-containing heterodimers is much higher than in double-stranded DNA or RNA. Therefore, PNA- and LNA-based biosensors have unprecedented sensitivity and specificity, with special applicability in DNA genotyping. Herein, the most relevant PNA- and LNA-based biosensors are presented, and their advantages and their current limitations are discussed. Some of the reviewed technology, while promising, still needs to bridge the gap between experimental status and the harder reality of biotechnological or biomedical applications. More... »

PAGES

3071-3089

References to SciGraph publications

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  • 1990-08. In vitro selection of RNA molecules that bind specific ligands in NATURE
  • 2009-01-28. Evaluation of locked nucleic acids for signal enhancement of oligonucleotide probes for microalgae immobilised on solid surfaces in JOURNAL OF APPLIED PHYCOLOGY
  • 2004-07-01. Peptide Nucleic Acid Microarrays in BIOCONJUGATION PROTOCOLS
  • 2010-10-29. Nucleic acid-based fluorescent probes and their analytical potential in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 2010-09-20. Comparison of the performance of the PANArray™ HPV test and DNA chip test for genotyping of human papillomavirus in cervical swabs in BIOCHIP JOURNAL
  • 2010. Recognition Receptors in Biosensors in NONE
  • 2008-04-20. Label-free detection of DNA hybridization based on hydration-induced tension in nucleic acid films in NATURE NANOTECHNOLOGY
  • 1997-02. Crystal structure of a peptide nucleic acid (PNA) duplex at 1.7 Å resolution in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 1993-08-31. Oligonucleotide Analogs with Dimethylenesulfide, -sulfoxide, and -sulfone Groups Replacing Phosphodiester Linkages in PROTOCOLS FOR OLIGONUCLEOTIDES AND ANALOGS
  • 2009. Biosensors and Biodetection, Methods and Protocols: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors in NONE
  • 1993-10. PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules in NATURE
  • 2004-10-21. Microarray-based, high-throughput gene expression profiling of microRNAs in NATURE METHODS
  • 2009-06-29. Self-Assembly of PNA-Encoded Peptides into Microarrays in PEPTIDE MICROARRAYS
  • 2009. Biosensors and Biodetection in NONE
  • 2010-08-28. Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs in MICRORNA AND CANCER
  • 2003. Surface Science, An Introduction in NONE
  • 2008-12-23. Label-free detection of DNA mutations by SPR: application to the early detection of inherited breast cancer in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 2010-06-20. Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 2009-01-05. Introduction to Microarray Technology in DNA MICROARRAYS FOR BIOMEDICAL RESEARCH
  • 1994-04. DNA-like double helix formed by peptide nucleic acid in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00216-012-5742-z

    DOI

    http://dx.doi.org/10.1007/s00216-012-5742-z

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22297860


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