Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-09-27

AUTHORS

Xinyi Wang, Mingjian Zou, Xiao Xu, Rong Lei, Kean Li, Na Li

ABSTRACT

The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs–KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20–800 nmol L−1 and 2 nmol L−1, respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94–104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs–KANA system, may provide a new approach for determination of compounds rich in OH groups.FigureSchematic diagram of KANA-induced aggregation of AuNPs and enhancement by urea, including magnification for illustration of N···H-O hydrogen bond between KANA and urea More... »

PAGES

2397-2403

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-009-3134-9

DOI

http://dx.doi.org/10.1007/s00216-009-3134-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000912312

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19784833


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