Analysis of glutathione adducts of patulin by means of liquid chromatography (HPLC) with biochemical detection (BCD) and electrospray ionization tandem ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-07

AUTHORS

Nils Helge Schebb, Helene Faber, Ronald Maul, Ferry Heus, Jeroen Kool, Hubertus Irth, Uwe Karst

ABSTRACT

A novel method for the identification of glutathione/electrophile adducts that are inhibiting glutathione-S-transferase (GST) activity was developed and applied for the analysis of the mycotoxin patulin. The method is based on high-performance liquid chromatography (HPLC) coupled to a continuous-flow enzyme reactor serving as biochemical detector (BCD) in parallel to electrospray mass spectrometric detection (ESI-MS). This HPLC-BCD technique combines a separation step and the detection of the inhibition and is therefore ideally suited for the analysis of the activity of single patulin/glutathione adducts within a complex mixture of adducts. Two out of at least 15 detected patulin-glutathione adducts showed strong GST inhibition. In ESI-MS, the inhibitory active adducts were characterized by [M + H]+ ions with m/z 462.1138 and m/z 741.2011, respectively. They could be identified as a dihydropyranone adduct containing one molecule glutathione and a ketohexanoic acid bearing two glutathione molecules. More... »

PAGES

1361-1373

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-009-2765-1

DOI

http://dx.doi.org/10.1007/s00216-009-2765-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019577492

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19390846


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276 schema:name Center for Cardiovascular Research (CCR), Institut für Pharmakologie, Charité-Universitätsmedizin Berlin, Hessische Straße 3-4, 10115, Berlin, Germany
277 rdf:type schema:Organization
 




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