Targeted next-generation sequencing by specific capture of multiple genomic loci using low-volume microfluidic DNA arrays View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-01

AUTHORS

Stephan Bau, Nadine Schracke, Marcel Kränzle, Haiguo Wu, Peer F. Stähler, Jörg D. Hoheisel, Markus Beier, Daniel Summerer

ABSTRACT

We report a flexible method for selective capture of sequence fragments from complex, eukaryotic genome libraries for next-generation sequencing based on hybridization to DNA microarrays. Using microfluidic array architecture and integrated hardware, the process is amenable to complete automation and does not introduce amplification steps into the standard library preparation workflow, thereby avoiding bias of sequence distribution and fragment lengths. We captured a discontiguous human genomic target region of 185 kb using a tiling design with 50mer probes. Analysis by high-throughput sequencing using an Illumina/Solexa 1G Genome Analyzer revealed 2150-fold enrichment with mean per base coverage between 4.6 and 107.5-fold for the individual target regions. This method represents a flexible and cost-effective approach for large-scale resequencing of complex genomes. More... »

PAGES

171-175

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-008-2460-7

DOI

http://dx.doi.org/10.1007/s00216-008-2460-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011118938

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18958448


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