Asynchronous activation of calcium and potassium currents by isoproterenol in canine ventricular myocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-02-25

AUTHORS

Ferenc Ruzsnavszky, Bence Hegyi, Kornél Kistamás, Krisztina Váczi, Balázs Horváth, Norbert Szentandrássy, Tamás Bányász, Péter P. Nánási, János Magyar

ABSTRACT

Adrenergic activation of L-type Ca2+ and various K+ currents is a crucial mechanism of cardiac adaptation; however, it may carry a substantial proarrhythmic risk as well. The aim of the present work was to study the timing of activation of Ca2+ and K+ currents in isolated canine ventricular cells in response to exposure to isoproterenol (ISO). Whole cell configuration of the patch-clamp technique in either conventional voltage clamp or action potential voltage clamp modes were used to monitor ICa, IKs, and IKr, while action potentials were recorded using sharp microelectrodes. ISO (10 nM) elevated the plateau potential and shortened action potential duration (APD) in subepicardial and mid-myocardial cells, which effects were associated with multifold enhancement of ICa and IKs and moderate stimulation of IKr. The ISO-induced plateau shift and ICa increase developed faster than the shortening of APD and stimulation of IKs and IKr. Blockade of β1-adrenoceptors (using 300 nM CGP-20712A) converted the ISO-induced shortening of APD to lengthening, decreased its latency, and reduced the plateau shift. In contrast, blockade of β2-adrenoceptors (by 50 nM ICI 118,551) augmented the APD-shortening effect and increased the latency of plateau shift without altering its magnitude. All effects of ISO were prevented by simultaneous blockade of both receptor types. Inhibition of phosphodiesterases decreased the differences observed in the turn on of the ISO-induced plateau shift and APD shortening. ISO-induced activation of ICa is turned on faster than the stimulation of IKs and IKr in canine ventricular cells due to the involvement of different adrenergic pathways and compartmentalization. More... »

PAGES

457-467

References to SciGraph publications

  • 1993-08. A comparative analysis of the time course of cardiac Ca2+ current response to rapid applications of β-adrenergic and dihydropyridine agonists in NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY
  • 1987-04. β2-Adrenoceptor-mediated positive inotropic effect of adrenaline in human ventricular myocardium in NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY
  • 1988-02. β-Adrenergic modulation in the heart in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2010-07-29. Effects of ropinirole on action potential characteristics and the underlying ion currents in canine ventricular myocytes in NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY
  • 2005-06-11. Asymmetrical distribution of ion channels in canine and human left-ventricular wall: epicardium versus midmyocardium in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2012-12-15. Sex differences in repolarization and slow delayed rectifier potassium current and their regulation by sympathetic stimulation in rabbits in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1985-10. Direct labelling of β2-adrenoceptors in NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY
  • 2012-12-19. Effects of tacrolimus on action potential configuration and transmembrane ion currents in canine ventricular cells in NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY
  • 1990-05. Ionic currents contributing to the action potential in single ventricular myocytes of the guinea pig studied with action potential clamp in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2008-07-05. Contribution of IKr and IK1 to ventricular repolarization in canine and human myocytes: is there any influence of action potential duration? in BASIC RESEARCH IN CARDIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00210-014-0964-6

    DOI

    http://dx.doi.org/10.1007/s00210-014-0964-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032373145

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24566722


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Neurosciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Action Potentials", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Calcium Channels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dogs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Heart Ventricles", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Isoproterenol", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Myocytes, Cardiac", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Potassium Channels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Receptors, Adrenergic, beta", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ruzsnavszky", 
            "givenName": "Ferenc", 
            "id": "sg:person.0605666537.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605666537.87"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hegyi", 
            "givenName": "Bence", 
            "id": "sg:person.01031111403.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031111403.81"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kistam\u00e1s", 
            "givenName": "Korn\u00e9l", 
            "id": "sg:person.01076341306.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076341306.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "V\u00e1czi", 
            "givenName": "Krisztina", 
            "id": "sg:person.01113702713.15", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113702713.15"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Horv\u00e1th", 
            "givenName": "Bal\u00e1zs", 
            "id": "sg:person.01033572077.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033572077.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Dental Physiology and Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
                "Department of Dental Physiology and Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Szentandr\u00e1ssy", 
            "givenName": "Norbert", 
            "id": "sg:person.0741603700.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741603700.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "B\u00e1ny\u00e1sz", 
            "givenName": "Tam\u00e1s", 
            "id": "sg:person.0772313221.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772313221.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Dental Physiology and Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
                "Department of Dental Physiology and Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "N\u00e1n\u00e1si", 
            "givenName": "P\u00e9ter P.", 
            "id": "sg:person.0724365203.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724365203.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.7122.6", 
              "name": [
                "Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Magyar", 
            "givenName": "J\u00e1nos", 
            "id": "sg:person.01206027354.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206027354.83"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00392058", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046545723", 
              "https://doi.org/10.1007/bf00392058"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00164799", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042364666", 
              "https://doi.org/10.1007/bf00164799"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00582323", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031834109", 
              "https://doi.org/10.1007/bf00582323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00424-012-1193-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001354751", 
              "https://doi.org/10.1007/s00424-012-1193-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00165555", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008023000", 
              "https://doi.org/10.1007/bf00165555"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00210-010-0538-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031160831", 
              "https://doi.org/10.1007/s00210-010-0538-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00210-012-0823-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025688667", 
              "https://doi.org/10.1007/s00210-012-0823-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00395-008-0730-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005477760", 
              "https://doi.org/10.1007/s00395-008-0730-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00498850", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028453319", 
              "https://doi.org/10.1007/bf00498850"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00424-005-1445-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028069154", 
              "https://doi.org/10.1007/s00424-005-1445-z"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2014-02-25", 
        "datePublishedReg": "2014-02-25", 
        "description": "Adrenergic activation of L-type Ca2+ and various K+ currents is a crucial mechanism of cardiac adaptation; however, it may carry a substantial proarrhythmic risk as well. The aim of the present work was to study the timing of activation of Ca2+ and K+ currents in isolated canine ventricular cells in response to exposure to isoproterenol (ISO). Whole cell configuration of the patch-clamp technique in either conventional voltage clamp or action potential voltage clamp modes were used to monitor ICa, IKs, and IKr, while action potentials were recorded using sharp microelectrodes. ISO (10\u00a0nM) elevated the plateau potential and shortened action potential duration (APD) in subepicardial and mid-myocardial cells, which effects were associated with multifold enhancement of ICa and IKs and moderate stimulation of IKr. The ISO-induced plateau shift and ICa increase developed faster than the shortening of APD and stimulation of IKs and IKr. Blockade of \u03b21-adrenoceptors (using 300\u00a0nM CGP-20712A) converted the ISO-induced shortening of APD to lengthening, decreased its latency, and reduced the plateau shift. In contrast, blockade of \u03b22-adrenoceptors (by 50\u00a0nM ICI 118,551) augmented the APD-shortening effect and increased the latency of plateau shift without altering its magnitude. All effects of ISO were prevented by simultaneous blockade of both receptor types. Inhibition of phosphodiesterases decreased the differences observed in the turn on of the ISO-induced plateau shift and APD shortening. ISO-induced activation of ICa is turned on faster than the stimulation of IKs and IKr in canine ventricular cells due to the involvement of different adrenergic pathways and compartmentalization.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s00210-014-0964-6", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1015404", 
            "issn": [
              "0028-1298", 
              "1432-1912"
            ], 
            "name": "Naunyn-Schmiedeberg's Archives of Pharmacology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "387"
          }
        ], 
        "keywords": [
          "action potential duration", 
          "canine ventricular cells", 
          "shortening of APD", 
          "ventricular cells", 
          "conventional voltage clamp", 
          "ISO-induced activation", 
          "inhibition of phosphodiesterases", 
          "voltage-clamp mode", 
          "patch-clamp technique", 
          "effects of ISO", 
          "whole-cell configuration", 
          "canine ventricular myocytes", 
          "APD shortening", 
          "proarrhythmic risk", 
          "cardiac adaptation", 
          "\u03b21-adrenoceptors", 
          "type Ca2", 
          "adrenergic pathways", 
          "simultaneous blockade", 
          "adrenergic activation", 
          "mid-myocardial cells", 
          "\u03b22-adrenoceptors", 
          "potential duration", 
          "potassium current", 
          "receptor types", 
          "action potentials", 
          "ventricular myocytes", 
          "clamp mode", 
          "plateau potentials", 
          "sharp microelectrodes", 
          "IKr", 
          "blockade", 
          "moderate stimulation", 
          "asynchronous activation", 
          "voltage clamp", 
          "stimulation", 
          "IKs", 
          "timing of activation", 
          "ICa increase", 
          "activation", 
          "shortening", 
          "cells", 
          "ICA", 
          "crucial mechanism", 
          "Ca2", 
          "isoproterenol", 
          "myocytes", 
          "latency", 
          "risk", 
          "effect", 
          "clamp", 
          "inhibition", 
          "exposure", 
          "duration", 
          "involvement", 
          "calcium", 
          "IK", 
          "aim", 
          "pathway", 
          "phosphodiesterases", 
          "response", 
          "differences", 
          "microelectrodes", 
          "increase", 
          "potential", 
          "ISO", 
          "contrast", 
          "timing", 
          "mechanism", 
          "compartmentalization", 
          "types", 
          "adaptation", 
          "shift", 
          "enhancement", 
          "technique", 
          "plateau", 
          "turn", 
          "magnitude", 
          "current", 
          "present work", 
          "cell configuration", 
          "mode", 
          "work", 
          "multifold enhancement", 
          "configuration", 
          "substantial proarrhythmic risk", 
          "isolated canine ventricular cells", 
          "action potential voltage clamp modes", 
          "potential voltage clamp modes", 
          "ISO-induced plateau shift", 
          "plateau shift", 
          "stimulation of IKs", 
          "ISO-induced shortening", 
          "latency of plateau", 
          "different adrenergic pathways"
        ], 
        "name": "Asynchronous activation of calcium and potassium currents by isoproterenol in canine ventricular myocytes", 
        "pagination": "457-467", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1032373145"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00210-014-0964-6"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24566722"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00210-014-0964-6", 
          "https://app.dimensions.ai/details/publication/pub.1032373145"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-01-01T18:32", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_626.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s00210-014-0964-6"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00210-014-0964-6'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00210-014-0964-6'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00210-014-0964-6'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00210-014-0964-6'


     

    This table displays all metadata directly associated to this object as RDF triples.

    299 TRIPLES      22 PREDICATES      142 URIs      124 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00210-014-0964-6 schema:about N0bb26d6236164f2488867d5e722f2ccb
    2 N4e651ff05821438292ddafaee141e6d8
    3 N5ea79aa2cc0643f682e0cbfc4b99ed92
    4 N8952fbffcf9e4e17b749afffbaad4090
    5 N92ca7ef6baa442189e515a3b18806a48
    6 Nac923637ac8845029f91fd3b55481c4f
    7 Nb972586a23ee430bacb2a6d4d24ee599
    8 Nbb6f5b6af3e74c6fae04ec996e8a7c41
    9 Nd2d5c1fa296f4910a4bd368d4cf6e21e
    10 Nde6f33293a474e96b65892e8083372bf
    11 Nfbdde1d1a4654cab8e69ce002bf59138
    12 anzsrc-for:11
    13 anzsrc-for:1109
    14 schema:author N4b11efa5469845b3b12f84dd28c26584
    15 schema:citation sg:pub.10.1007/bf00164799
    16 sg:pub.10.1007/bf00165555
    17 sg:pub.10.1007/bf00392058
    18 sg:pub.10.1007/bf00498850
    19 sg:pub.10.1007/bf00582323
    20 sg:pub.10.1007/s00210-010-0538-1
    21 sg:pub.10.1007/s00210-012-0823-2
    22 sg:pub.10.1007/s00395-008-0730-3
    23 sg:pub.10.1007/s00424-005-1445-z
    24 sg:pub.10.1007/s00424-012-1193-9
    25 schema:datePublished 2014-02-25
    26 schema:datePublishedReg 2014-02-25
    27 schema:description Adrenergic activation of L-type Ca2+ and various K+ currents is a crucial mechanism of cardiac adaptation; however, it may carry a substantial proarrhythmic risk as well. The aim of the present work was to study the timing of activation of Ca2+ and K+ currents in isolated canine ventricular cells in response to exposure to isoproterenol (ISO). Whole cell configuration of the patch-clamp technique in either conventional voltage clamp or action potential voltage clamp modes were used to monitor ICa, IKs, and IKr, while action potentials were recorded using sharp microelectrodes. ISO (10 nM) elevated the plateau potential and shortened action potential duration (APD) in subepicardial and mid-myocardial cells, which effects were associated with multifold enhancement of ICa and IKs and moderate stimulation of IKr. The ISO-induced plateau shift and ICa increase developed faster than the shortening of APD and stimulation of IKs and IKr. Blockade of β1-adrenoceptors (using 300 nM CGP-20712A) converted the ISO-induced shortening of APD to lengthening, decreased its latency, and reduced the plateau shift. In contrast, blockade of β2-adrenoceptors (by 50 nM ICI 118,551) augmented the APD-shortening effect and increased the latency of plateau shift without altering its magnitude. All effects of ISO were prevented by simultaneous blockade of both receptor types. Inhibition of phosphodiesterases decreased the differences observed in the turn on of the ISO-induced plateau shift and APD shortening. ISO-induced activation of ICa is turned on faster than the stimulation of IKs and IKr in canine ventricular cells due to the involvement of different adrenergic pathways and compartmentalization.
    28 schema:genre article
    29 schema:inLanguage en
    30 schema:isAccessibleForFree false
    31 schema:isPartOf Ndd78c56acc0b46d78145a523a1673b3b
    32 Nf77930f378bf4204aeb1c87a9b444164
    33 sg:journal.1015404
    34 schema:keywords APD shortening
    35 Ca2
    36 ICA
    37 ICa increase
    38 IK
    39 IKr
    40 IKs
    41 ISO
    42 ISO-induced activation
    43 ISO-induced plateau shift
    44 ISO-induced shortening
    45 action potential duration
    46 action potential voltage clamp modes
    47 action potentials
    48 activation
    49 adaptation
    50 adrenergic activation
    51 adrenergic pathways
    52 aim
    53 asynchronous activation
    54 blockade
    55 calcium
    56 canine ventricular cells
    57 canine ventricular myocytes
    58 cardiac adaptation
    59 cell configuration
    60 cells
    61 clamp
    62 clamp mode
    63 compartmentalization
    64 configuration
    65 contrast
    66 conventional voltage clamp
    67 crucial mechanism
    68 current
    69 differences
    70 different adrenergic pathways
    71 duration
    72 effect
    73 effects of ISO
    74 enhancement
    75 exposure
    76 increase
    77 inhibition
    78 inhibition of phosphodiesterases
    79 involvement
    80 isolated canine ventricular cells
    81 isoproterenol
    82 latency
    83 latency of plateau
    84 magnitude
    85 mechanism
    86 microelectrodes
    87 mid-myocardial cells
    88 mode
    89 moderate stimulation
    90 multifold enhancement
    91 myocytes
    92 patch-clamp technique
    93 pathway
    94 phosphodiesterases
    95 plateau
    96 plateau potentials
    97 plateau shift
    98 potassium current
    99 potential
    100 potential duration
    101 potential voltage clamp modes
    102 present work
    103 proarrhythmic risk
    104 receptor types
    105 response
    106 risk
    107 sharp microelectrodes
    108 shift
    109 shortening
    110 shortening of APD
    111 simultaneous blockade
    112 stimulation
    113 stimulation of IKs
    114 substantial proarrhythmic risk
    115 technique
    116 timing
    117 timing of activation
    118 turn
    119 type Ca2
    120 types
    121 ventricular cells
    122 ventricular myocytes
    123 voltage clamp
    124 voltage-clamp mode
    125 whole-cell configuration
    126 work
    127 β1-adrenoceptors
    128 β2-adrenoceptors
    129 schema:name Asynchronous activation of calcium and potassium currents by isoproterenol in canine ventricular myocytes
    130 schema:pagination 457-467
    131 schema:productId N19dba120f0414bfb9f6e46fb19d3e89f
    132 N3b81322d30c64fc7975e769b3b3d05dc
    133 N7358e610fb6a4d37bc1dfe4ec76caf79
    134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032373145
    135 https://doi.org/10.1007/s00210-014-0964-6
    136 schema:sdDatePublished 2022-01-01T18:32
    137 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    138 schema:sdPublisher N295806f0eb214d27bba4143643c2d320
    139 schema:url https://doi.org/10.1007/s00210-014-0964-6
    140 sgo:license sg:explorer/license/
    141 sgo:sdDataset articles
    142 rdf:type schema:ScholarlyArticle
    143 N0ba700347f7d4931985bb944c2f9bce7 rdf:first sg:person.0724365203.35
    144 rdf:rest N4e0763d3742e41f59dfe789490a00e14
    145 N0bb26d6236164f2488867d5e722f2ccb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Potassium Channels
    147 rdf:type schema:DefinedTerm
    148 N19af0385dab0448983d795b8e8bded3e rdf:first sg:person.0741603700.70
    149 rdf:rest N267cf68562cd41a8a636b3afad658480
    150 N19dba120f0414bfb9f6e46fb19d3e89f schema:name pubmed_id
    151 schema:value 24566722
    152 rdf:type schema:PropertyValue
    153 N267cf68562cd41a8a636b3afad658480 rdf:first sg:person.0772313221.04
    154 rdf:rest N0ba700347f7d4931985bb944c2f9bce7
    155 N295806f0eb214d27bba4143643c2d320 schema:name Springer Nature - SN SciGraph project
    156 rdf:type schema:Organization
    157 N3b81322d30c64fc7975e769b3b3d05dc schema:name dimensions_id
    158 schema:value pub.1032373145
    159 rdf:type schema:PropertyValue
    160 N4b11efa5469845b3b12f84dd28c26584 rdf:first sg:person.0605666537.87
    161 rdf:rest Nf4b74887eda443c4b6151c19b889a118
    162 N4e0763d3742e41f59dfe789490a00e14 rdf:first sg:person.01206027354.83
    163 rdf:rest rdf:nil
    164 N4e651ff05821438292ddafaee141e6d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    165 schema:name Myocytes, Cardiac
    166 rdf:type schema:DefinedTerm
    167 N5ea79aa2cc0643f682e0cbfc4b99ed92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Animals
    169 rdf:type schema:DefinedTerm
    170 N7358e610fb6a4d37bc1dfe4ec76caf79 schema:name doi
    171 schema:value 10.1007/s00210-014-0964-6
    172 rdf:type schema:PropertyValue
    173 N8952fbffcf9e4e17b749afffbaad4090 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Female
    175 rdf:type schema:DefinedTerm
    176 N8cd204e99eb94fefb90d9209ea9cd6b1 rdf:first sg:person.01033572077.22
    177 rdf:rest N19af0385dab0448983d795b8e8bded3e
    178 N92ca7ef6baa442189e515a3b18806a48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Heart Ventricles
    180 rdf:type schema:DefinedTerm
    181 Nac923637ac8845029f91fd3b55481c4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Male
    183 rdf:type schema:DefinedTerm
    184 Nb972586a23ee430bacb2a6d4d24ee599 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Calcium Channels
    186 rdf:type schema:DefinedTerm
    187 Nbb6f5b6af3e74c6fae04ec996e8a7c41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name Action Potentials
    189 rdf:type schema:DefinedTerm
    190 Nd13c9b6f1755494db1b7570297895560 rdf:first sg:person.01076341306.25
    191 rdf:rest Ndb2841ce4af549f5888bf144a4329422
    192 Nd2d5c1fa296f4910a4bd368d4cf6e21e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Dogs
    194 rdf:type schema:DefinedTerm
    195 Ndb2841ce4af549f5888bf144a4329422 rdf:first sg:person.01113702713.15
    196 rdf:rest N8cd204e99eb94fefb90d9209ea9cd6b1
    197 Ndd78c56acc0b46d78145a523a1673b3b schema:volumeNumber 387
    198 rdf:type schema:PublicationVolume
    199 Nde6f33293a474e96b65892e8083372bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    200 schema:name Receptors, Adrenergic, beta
    201 rdf:type schema:DefinedTerm
    202 Nf4b74887eda443c4b6151c19b889a118 rdf:first sg:person.01031111403.81
    203 rdf:rest Nd13c9b6f1755494db1b7570297895560
    204 Nf77930f378bf4204aeb1c87a9b444164 schema:issueNumber 5
    205 rdf:type schema:PublicationIssue
    206 Nfbdde1d1a4654cab8e69ce002bf59138 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    207 schema:name Isoproterenol
    208 rdf:type schema:DefinedTerm
    209 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    210 schema:name Medical and Health Sciences
    211 rdf:type schema:DefinedTerm
    212 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
    213 schema:name Neurosciences
    214 rdf:type schema:DefinedTerm
    215 sg:journal.1015404 schema:issn 0028-1298
    216 1432-1912
    217 schema:name Naunyn-Schmiedeberg's Archives of Pharmacology
    218 schema:publisher Springer Nature
    219 rdf:type schema:Periodical
    220 sg:person.01031111403.81 schema:affiliation grid-institutes:grid.7122.6
    221 schema:familyName Hegyi
    222 schema:givenName Bence
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031111403.81
    224 rdf:type schema:Person
    225 sg:person.01033572077.22 schema:affiliation grid-institutes:grid.7122.6
    226 schema:familyName Horváth
    227 schema:givenName Balázs
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033572077.22
    229 rdf:type schema:Person
    230 sg:person.01076341306.25 schema:affiliation grid-institutes:grid.7122.6
    231 schema:familyName Kistamás
    232 schema:givenName Kornél
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076341306.25
    234 rdf:type schema:Person
    235 sg:person.01113702713.15 schema:affiliation grid-institutes:grid.7122.6
    236 schema:familyName Váczi
    237 schema:givenName Krisztina
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113702713.15
    239 rdf:type schema:Person
    240 sg:person.01206027354.83 schema:affiliation grid-institutes:grid.7122.6
    241 schema:familyName Magyar
    242 schema:givenName János
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206027354.83
    244 rdf:type schema:Person
    245 sg:person.0605666537.87 schema:affiliation grid-institutes:grid.7122.6
    246 schema:familyName Ruzsnavszky
    247 schema:givenName Ferenc
    248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605666537.87
    249 rdf:type schema:Person
    250 sg:person.0724365203.35 schema:affiliation grid-institutes:grid.7122.6
    251 schema:familyName Nánási
    252 schema:givenName Péter P.
    253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724365203.35
    254 rdf:type schema:Person
    255 sg:person.0741603700.70 schema:affiliation grid-institutes:grid.7122.6
    256 schema:familyName Szentandrássy
    257 schema:givenName Norbert
    258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741603700.70
    259 rdf:type schema:Person
    260 sg:person.0772313221.04 schema:affiliation grid-institutes:grid.7122.6
    261 schema:familyName Bányász
    262 schema:givenName Tamás
    263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772313221.04
    264 rdf:type schema:Person
    265 sg:pub.10.1007/bf00164799 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042364666
    266 https://doi.org/10.1007/bf00164799
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1007/bf00165555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008023000
    269 https://doi.org/10.1007/bf00165555
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1007/bf00392058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046545723
    272 https://doi.org/10.1007/bf00392058
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1007/bf00498850 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028453319
    275 https://doi.org/10.1007/bf00498850
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1007/bf00582323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031834109
    278 https://doi.org/10.1007/bf00582323
    279 rdf:type schema:CreativeWork
    280 sg:pub.10.1007/s00210-010-0538-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031160831
    281 https://doi.org/10.1007/s00210-010-0538-1
    282 rdf:type schema:CreativeWork
    283 sg:pub.10.1007/s00210-012-0823-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025688667
    284 https://doi.org/10.1007/s00210-012-0823-2
    285 rdf:type schema:CreativeWork
    286 sg:pub.10.1007/s00395-008-0730-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005477760
    287 https://doi.org/10.1007/s00395-008-0730-3
    288 rdf:type schema:CreativeWork
    289 sg:pub.10.1007/s00424-005-1445-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1028069154
    290 https://doi.org/10.1007/s00424-005-1445-z
    291 rdf:type schema:CreativeWork
    292 sg:pub.10.1007/s00424-012-1193-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001354751
    293 https://doi.org/10.1007/s00424-012-1193-9
    294 rdf:type schema:CreativeWork
    295 grid-institutes:grid.7122.6 schema:alternateName Department of Dental Physiology and Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
    296 Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary
    297 schema:name Department of Dental Physiology and Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
    298 Department of Physiology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, 4012, Debrecen, Hungary
    299 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...