Mouse innate-like B-1 lymphocytes promote inhaled particle-induced in vitro granuloma formation and inflammation in conjunction with macrophages View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2021-12-21

AUTHORS

Léa Hiéronimus, Raïssa Demazy, Laura Christiaens, Francine Uwambayinema, Jean-François Geuens, Youssof Yacoub, François Huaux

ABSTRACT

The current paradigm for explaining lung granulomatous diseases induced by inhaled particles is mainly based on macrophages. This mechanism is now challenging because B lymphocytes also infiltrate injured tissue, and the deficiency in B lymphocytes is associated with limited lung granulomas in silica-treated mice. Here, we investigated how B lymphocytes respond to micro- and nanoparticles by combining in vivo and in vitro mouse models. We first demonstrated that innate-like B-1 lymphocytes (not conventional B-2 lymphocytes or plasma cells) specifically accumulated during granuloma formation in mice instilled with crystalline silica (DQ12, 2.5 mg/mouse) and carbon nanotubes (CNT Mitsui, 0.2 mg/mouse). In comparison to macrophages, peritoneal B-1 lymphocytes purified from naïve mice were resistant to the pyroptotic activity of reactive particles (up to 1 mg/mL) but clustered to establish in vitro cell/particle aggregates. Mouse B-1 lymphocytes (not B-2 lymphocytes) in coculture with macrophages and CNT (0.1 µg/mL) organized three-dimensional spheroid structures in Matrigel and stimulated the release of TIMP-1. Furthermore, purified B-1 lymphocytes are sensitive to nanosilica toxicity through radical generation in culture. Nanosilica-exposed B-1 lymphocytes released proinflammatory cytokines and alarmins. In conclusion, our data indicate that in addition to macrophages, B-1 lymphocytes participate in micrometric particle-induced granuloma formation and display inflammatory functions in response to nanoparticles. More... »

PAGES

585-599

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00204-021-03200-2

DOI

http://dx.doi.org/10.1007/s00204-021-03200-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1144049592

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/34935064


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70 lymphocytes
71 macrophages
72 mechanism
73 mice
74 micro
75 model
76 mouse B
77 mouse model
78 nanoparticles
79 nanosilica
80 nanotubes
81 naïve mice
82 paradigm
83 particle aggregates
84 particles
85 proinflammatory cytokines
86 pyroptotic activity
87 radical generation
88 reactive particles
89 release
90 response
91 silica
92 silica-treated mice
93 spheroid structures
94 structure
95 three-dimensional spheroid structures
96 tissue
97 toxicity
98 vivo
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