Ontology type: schema:ScholarlyArticle
2015-01
AUTHORSDi Zhou, Ge Lin, Si-Cong Zeng, Bo Xiong, Ping-Yuan Xie, De-Hua Cheng, Qing Zheng, Qi Ouyang, Xiao-Ying Zhou, Wei-Ling Tang, Yi Sun, Guang-Ying Lu, Guang-Xiu Lu
ABSTRACTHow to maintain the genetic integrity of cultured human embryonic stem (hES) cells is raising crucial concerns for future clinical use in regenerative medicine. Mitomycin C(MMC), a DNA damage agent, is widely used for preparation of feeder cells in many laboratories. However, to what extent MMC affects the karyotypic stability of hES cells is not clear. Here, we measured residual MMC using High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry following each step of feeder preparation and found that 2.26 ± 0.77 and 3.50 ± 0.92 ng/ml remained in mouse feeder cells and human feeder cells, respectively. In addition, different amounts of MMC caused different chromosomal aberrations in hES cells. In particular, one abnormality, dup(1)(p32p36), was the same identical to one we previously reported in another hES cell line. Using Affymetrix SNP 6.0 arrays, the copy number variation changes of the hES cells maintained on MMC-inactivated feeders (MMC-feeder) were significantly more than those cultured on γ-inactivated feeder (IR-feeder) cells. Furthermore, DNA damage response (DDR) genes were down-regulated during long-term culture in the MMC-containing system, leading to DDR defect and shortened telomeres of hES cells, a sign of genomic instability. Therefore, MMC-feeder and MMC-induced genomic variation present an important safety problem that would limit such hES from being applied for future clinic use and drug screening. More... »
PAGES33-45
http://scigraph.springernature.com/pub.10.1007/s00204-014-1250-6
DOIhttp://dx.doi.org/10.1007/s00204-014-1250-6
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1015549674
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24838295
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Apoptosis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Culture Techniques",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Cycle",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cells, Cultured",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromatography, High Pressure Liquid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Damage",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Embryonic Stem Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genomic Instability",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Karyotyping",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mitomycin",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polymerase Chain Reaction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tandem Mass Spectrometry",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Zhou",
"givenName": "Di",
"id": "sg:person.01336621165.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336621165.48"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Lin",
"givenName": "Ge",
"id": "sg:person.01335145024.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335145024.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Zeng",
"givenName": "Si-Cong",
"id": "sg:person.01073504152.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073504152.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Xiong",
"givenName": "Bo",
"id": "sg:person.0663515224.20",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663515224.20"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central South University",
"id": "https://www.grid.ac/institutes/grid.216417.7",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Xie",
"givenName": "Ping-Yuan",
"id": "sg:person.01224557524.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224557524.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Cheng",
"givenName": "De-Hua",
"id": "sg:person.0614362011.14",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614362011.14"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Zheng",
"givenName": "Qing",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Ouyang",
"givenName": "Qi",
"id": "sg:person.0774102524.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774102524.43"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Zhou",
"givenName": "Xiao-Ying",
"id": "sg:person.01070162761.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070162761.08"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Tang",
"givenName": "Wei-Ling",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Sun",
"givenName": "Yi",
"id": "sg:person.01123674424.22",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123674424.22"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Lu",
"givenName": "Guang-Ying",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Reproductive & Genetic Hospital CITIC-Xiangya",
"id": "https://www.grid.ac/institutes/grid.477823.d",
"name": [
"Institute of Reproduction and Stem Cell Engineering, Central South University, 8 Luyun Road, 410000, Changsha, Hunan, China",
"National Engineering and Research Center of Human Stem Cell, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, 8 Luyun Road, 410000, Changsha, Hunan, China",
"Reproductive and Genetic Hospital of CITIC-Xiangya, 88 Xiangya Road, 410078, Changsha, Hunan, China"
],
"type": "Organization"
},
"familyName": "Lu",
"givenName": "Guang-Xiu",
"id": "sg:person.01240123024.27",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240123024.27"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1242/jcs.131433",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000130494"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0960-9822(00)00483-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002193353"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0163-7258(97)00088-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005216528"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.1615",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005590339",
"https://doi.org/10.1038/nbt.1615"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt922",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005956847",
"https://doi.org/10.1038/nbt922"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt922",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005956847",
"https://doi.org/10.1038/nbt922"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt726",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009583211",
"https://doi.org/10.1038/nbt726"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt726",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009583211",
"https://doi.org/10.1038/nbt726"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0404-381",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009913524",
"https://doi.org/10.1038/nbt0404-381"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0404-381",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009913524",
"https://doi.org/10.1038/nbt0404-381"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011283350",
"https://doi.org/10.1038/nbt1102"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011283350",
"https://doi.org/10.1038/nbt1102"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011283350",
"https://doi.org/10.1038/nbt1102"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1285",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012224204",
"https://doi.org/10.1038/nbt1285"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng1631",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013244477",
"https://doi.org/10.1038/ng1631"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng1631",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013244477",
"https://doi.org/10.1038/ng1631"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cdd.2010.106",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016535373",
"https://doi.org/10.1038/cdd.2010.106"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cdd.2010.106",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016535373",
"https://doi.org/10.1038/cdd.2010.106"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/292154a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017487986",
"https://doi.org/10.1038/292154a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/gcc.20574",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017530700"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jcp.21735",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018206895"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jcp.21735",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018206895"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jpba.2005.06.024",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019535220"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.78.12.7634",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020059945"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1056/nejmsr040330",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022304644"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.mrfmmm.2011.02.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335812"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.mrfmmm.2011.02.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335812"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.mrfmmm.2011.02.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335812"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.mrfmmm.2011.02.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335812"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.mrfmmm.2011.02.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335812"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/376785a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022545215",
"https://doi.org/10.1038/376785a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/387520a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029483387",
"https://doi.org/10.1038/387520a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/387516a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031716517",
"https://doi.org/10.1038/387516a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1182/blood-2006-10-054247",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031752720"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/74447",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033604670",
"https://doi.org/10.1038/74447"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/74447",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033604670",
"https://doi.org/10.1038/74447"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11626-010-9307-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035290680",
"https://doi.org/10.1007/s11626-010-9307-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11626-010-9307-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035290680",
"https://doi.org/10.1007/s11626-010-9307-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0105-19",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038041341",
"https://doi.org/10.1038/nbt0105-19"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0105-19",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038041341",
"https://doi.org/10.1038/nbt0105-19"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc2248",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039629976",
"https://doi.org/10.1038/nrc2248"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/stem.123",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041486268"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/stem.123",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041486268"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cellbi.2009.04.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042924806"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jcp.20776",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044783559"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.96.8.4494",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049662628"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.23.2.754-761.2003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050622610"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/scd.2007.0186",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059309743"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075690597",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3969/j.issn.1672-7347.2010.11.",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1078311108"
],
"type": "CreativeWork"
}
],
"datePublished": "2015-01",
"datePublishedReg": "2015-01-01",
"description": "How to maintain the genetic integrity of cultured human embryonic stem (hES) cells is raising crucial concerns for future clinical use in regenerative medicine. Mitomycin C(MMC), a DNA damage agent, is widely used for preparation of feeder cells in many laboratories. However, to what extent MMC affects the karyotypic stability of hES cells is not clear. Here, we measured residual MMC using High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry following each step of feeder preparation and found that 2.26 \u00b1 0.77 and 3.50 \u00b1 0.92 ng/ml remained in mouse feeder cells and human feeder cells, respectively. In addition, different amounts of MMC caused different chromosomal aberrations in hES cells. In particular, one abnormality, dup(1)(p32p36), was the same identical to one we previously reported in another hES cell line. Using Affymetrix SNP 6.0 arrays, the copy number variation changes of the hES cells maintained on MMC-inactivated feeders (MMC-feeder) were significantly more than those cultured on \u03b3-inactivated feeder (IR-feeder) cells. Furthermore, DNA damage response (DDR) genes were down-regulated during long-term culture in the MMC-containing system, leading to DDR defect and shortened telomeres of hES cells, a sign of genomic instability. Therefore, MMC-feeder and MMC-induced genomic variation present an important safety problem that would limit such hES from being applied for future clinic use and drug screening.",
"genre": "research_article",
"id": "sg:pub.10.1007/s00204-014-1250-6",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1019669",
"issn": [
"0340-5761",
"1432-0738"
],
"name": "Archives of Toxicology",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "89"
}
],
"name": "Trace levels of mitomycin C disrupt genomic integrity and lead to DNA damage response defect in long-term-cultured human embryonic stem cells",
"pagination": "33-45",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"4a16049d6772d2c10954f7b0a6e0617ffdee6db9af1108c5c88c94be738c6dde"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"24838295"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0417615"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00204-014-1250-6"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1015549674"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00204-014-1250-6",
"https://app.dimensions.ai/details/publication/pub.1015549674"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T02:00",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000511.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2Fs00204-014-1250-6"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00204-014-1250-6'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00204-014-1250-6'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00204-014-1250-6'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00204-014-1250-6'
This table displays all metadata directly associated to this object as RDF triples.
326 TRIPLES
21 PREDICATES
76 URIs
34 LITERALS
22 BLANK NODES