Distribution of ATPase and ATP-to-ADP phosphate exchange activities in magnesium chelatase subunits of Chlorobium vibrioforme and Synechocystis PCC6803 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-02

AUTHORS

Bent L. Petersen, C. G. Kannangara, Knud W. Henningsen

ABSTRACT

Insertion of magnesium into protoporphyrin IX is a complex ATP-dependent reaction catalysed by the enzyme Mg-chelatase. Three separate proteins (Mg-chelatase subunits), designated as D, H and I, are involved in the chelation reaction. The genes encoding the Mg-chelatase subunits of the green sulfur bacterium Chlorobium vibrioforme and of the cyanobacterium Synechocystis strain PCC6803 were expressed in Escherichia coli. The recombinant proteins were purified, tested for ATPase and phosphate exchange activities, and compared with the activities of the corresponding subunits of Rhodobacter sphaeroides. The Synechocystis strain PCC6803 I subunit and the C. vibrioforme H and I subunits hydrolysed ATP at the rates of 2.0, 1.8 and 0.16 nmol (mg protein)-1 min-1, respectively. The ATPase activity of the C. vibrioforme H subunit was similar to that reported for the R. sphaeroides H subunit. The Synechocystis strain PCC6803 H subunit failed to hydrolyse ATP. The I subunit of Synechocystis strain PCC6803 and C. vibrioforme catalysed a transfer of PO4 from ATP to ADP (exchange activity) at the rate of 1.75 +/- 0.15 nmol (mg protein)-1 min-1. This exchange rate was 300-fold lower than that reported for the R. sphaeroides I subunit. The PO4 exchange activities were correlated with the presence of the sequence GXRGTGKSTXVRALA in the primary structure of the three I subunits. Mg-chelatase activity was reconstituted by combining the three subunits of the same bacterium [rates of 41-89 pmol Mg-deuteroporphyrin (mg protein)-1 min-1]. Heterologous subunit combinations resulted in low or no Mg-chelatase activity. More... »

PAGES

146-150

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002030050692

DOI

http://dx.doi.org/10.1007/s002030050692

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034999230

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10201094


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Diphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chlorobi", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyanobacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lyases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphates", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Copenhagen", 
          "id": "https://www.grid.ac/institutes/grid.5254.6", 
          "name": [
            "Department of Ecology and Molecular Biology,  Royal Veterinary and Agricultural University,  Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark., DK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Petersen", 
        "givenName": "Bent L.", 
        "id": "sg:person.01126040223.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126040223.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Carlsberg Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.418674.8", 
          "name": [
            "Department of Physiology, Carlsberg Laboratory,  DK-2500 Valby, Copenhagen, Denmark e-mail: gamini@crc.dk Tel. +45-3327-5272; Fax +45-3327-4766, DK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kannangara", 
        "givenName": "C. G.", 
        "id": "sg:person.01156254575.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156254575.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Copenhagen", 
          "id": "https://www.grid.ac/institutes/grid.5254.6", 
          "name": [
            "Department of Ecology and Molecular Biology,  Royal Veterinary and Agricultural University,  Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark., DK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Henningsen", 
        "givenName": "Knud W.", 
        "id": "sg:person.0717526447.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717526447.08"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1999-02", 
    "datePublishedReg": "1999-02-01", 
    "description": "Insertion of magnesium into protoporphyrin IX is a complex ATP-dependent reaction catalysed by the enzyme Mg-chelatase. Three separate proteins (Mg-chelatase subunits), designated as D, H and I, are involved in the chelation reaction. The genes encoding the Mg-chelatase subunits of the green sulfur bacterium Chlorobium vibrioforme and of the cyanobacterium Synechocystis strain PCC6803 were expressed in Escherichia coli. The recombinant proteins were purified, tested for ATPase and phosphate exchange activities, and compared with the activities of the corresponding subunits of Rhodobacter sphaeroides. The Synechocystis strain PCC6803 I subunit and the C. vibrioforme H and I subunits hydrolysed ATP at the rates of 2.0, 1.8 and 0.16 nmol (mg protein)-1 min-1, respectively. The ATPase activity of the C. vibrioforme H subunit was similar to that reported for the R. sphaeroides H subunit. The Synechocystis strain PCC6803 H subunit failed to hydrolyse ATP. The I subunit of Synechocystis strain PCC6803 and C. vibrioforme catalysed a transfer of PO4 from ATP to ADP (exchange activity) at the rate of 1.75 +/- 0.15 nmol (mg protein)-1 min-1. This exchange rate was 300-fold lower than that reported for the R. sphaeroides I subunit. The PO4 exchange activities were correlated with the presence of the sequence GXRGTGKSTXVRALA in the primary structure of the three I subunits. Mg-chelatase activity was reconstituted by combining the three subunits of the same bacterium [rates of 41-89 pmol Mg-deuteroporphyrin (mg protein)-1 min-1]. Heterologous subunit combinations resulted in low or no Mg-chelatase activity.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002030050692", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018945", 
        "issn": [
          "0302-8933", 
          "1432-072X"
        ], 
        "name": "Archives of Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "171"
      }
    ], 
    "name": "Distribution of ATPase and ATP-to-ADP phosphate exchange activities in magnesium chelatase subunits of Chlorobium vibrioforme and Synechocystis PCC6803", 
    "pagination": "146-150", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a6b9205c5e1acc8d26ea4316fd51bf9649867a89bd2f7128d0f5dd83275246c0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10201094"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410427"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002030050692"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034999230"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002030050692", 
      "https://app.dimensions.ai/details/publication/pub.1034999230"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs002030050692"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002030050692'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002030050692'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002030050692'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002030050692'


 

This table displays all metadata directly associated to this object as RDF triples.

114 TRIPLES      20 PREDICATES      36 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002030050692 schema:about N10099950f46c4b158c789d21a841c89a
2 N133b00cf746a4edb96696e9f1d1cefdf
3 N3ccd601a1b0e4f7e8278cdf24b6bd868
4 N585c0d7891e64d3bb089a5f56e8ece4e
5 N901a601ada6041bca7bb80bd82c9b050
6 Na7bc72784c9b4fa5838e72c51a5dab3a
7 Nb413ff0812a24e909a834ffd958889af
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author Ne4fb795e678941dd92991831fa9a329e
11 schema:datePublished 1999-02
12 schema:datePublishedReg 1999-02-01
13 schema:description Insertion of magnesium into protoporphyrin IX is a complex ATP-dependent reaction catalysed by the enzyme Mg-chelatase. Three separate proteins (Mg-chelatase subunits), designated as D, H and I, are involved in the chelation reaction. The genes encoding the Mg-chelatase subunits of the green sulfur bacterium Chlorobium vibrioforme and of the cyanobacterium Synechocystis strain PCC6803 were expressed in Escherichia coli. The recombinant proteins were purified, tested for ATPase and phosphate exchange activities, and compared with the activities of the corresponding subunits of Rhodobacter sphaeroides. The Synechocystis strain PCC6803 I subunit and the C. vibrioforme H and I subunits hydrolysed ATP at the rates of 2.0, 1.8 and 0.16 nmol (mg protein)-1 min-1, respectively. The ATPase activity of the C. vibrioforme H subunit was similar to that reported for the R. sphaeroides H subunit. The Synechocystis strain PCC6803 H subunit failed to hydrolyse ATP. The I subunit of Synechocystis strain PCC6803 and C. vibrioforme catalysed a transfer of PO4 from ATP to ADP (exchange activity) at the rate of 1.75 +/- 0.15 nmol (mg protein)-1 min-1. This exchange rate was 300-fold lower than that reported for the R. sphaeroides I subunit. The PO4 exchange activities were correlated with the presence of the sequence GXRGTGKSTXVRALA in the primary structure of the three I subunits. Mg-chelatase activity was reconstituted by combining the three subunits of the same bacterium [rates of 41-89 pmol Mg-deuteroporphyrin (mg protein)-1 min-1]. Heterologous subunit combinations resulted in low or no Mg-chelatase activity.
14 schema:genre research_article
15 schema:inLanguage en
16 schema:isAccessibleForFree false
17 schema:isPartOf N6f14a74a4fc84bf6a253b069700acd54
18 Ne284918529014cf489d3555c159119df
19 sg:journal.1018945
20 schema:name Distribution of ATPase and ATP-to-ADP phosphate exchange activities in magnesium chelatase subunits of Chlorobium vibrioforme and Synechocystis PCC6803
21 schema:pagination 146-150
22 schema:productId N0105aa77334747deab78c05177c5ef57
23 N1e9fb7d4b2534f1f9e26546e96835fd3
24 Ne077d065acc847a8936ec2d15cb142f6
25 Nf717f9ffb3ad406e9416e7d7d8e157c3
26 Nfc2ac18625f34935a5eb4d86c1fc9282
27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034999230
28 https://doi.org/10.1007/s002030050692
29 schema:sdDatePublished 2019-04-10T15:52
30 schema:sdLicense https://scigraph.springernature.com/explorer/license/
31 schema:sdPublisher N34332931168746bdbf9e6d4732906063
32 schema:url http://link.springer.com/10.1007%2Fs002030050692
33 sgo:license sg:explorer/license/
34 sgo:sdDataset articles
35 rdf:type schema:ScholarlyArticle
36 N0105aa77334747deab78c05177c5ef57 schema:name dimensions_id
37 schema:value pub.1034999230
38 rdf:type schema:PropertyValue
39 N0413fa544f204875a0f3c3952be93b34 rdf:first sg:person.0717526447.08
40 rdf:rest rdf:nil
41 N10099950f46c4b158c789d21a841c89a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
42 schema:name Lyases
43 rdf:type schema:DefinedTerm
44 N133b00cf746a4edb96696e9f1d1cefdf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
45 schema:name Chlorobi
46 rdf:type schema:DefinedTerm
47 N1e9fb7d4b2534f1f9e26546e96835fd3 schema:name pubmed_id
48 schema:value 10201094
49 rdf:type schema:PropertyValue
50 N34332931168746bdbf9e6d4732906063 schema:name Springer Nature - SN SciGraph project
51 rdf:type schema:Organization
52 N3ccd601a1b0e4f7e8278cdf24b6bd868 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Adenosine Triphosphatases
54 rdf:type schema:DefinedTerm
55 N585c0d7891e64d3bb089a5f56e8ece4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Cyanobacteria
57 rdf:type schema:DefinedTerm
58 N654489d0e28d44d99d495163204fcb94 rdf:first sg:person.01156254575.47
59 rdf:rest N0413fa544f204875a0f3c3952be93b34
60 N6f14a74a4fc84bf6a253b069700acd54 schema:volumeNumber 171
61 rdf:type schema:PublicationVolume
62 N901a601ada6041bca7bb80bd82c9b050 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name Adenosine Diphosphate
64 rdf:type schema:DefinedTerm
65 Na7bc72784c9b4fa5838e72c51a5dab3a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Adenosine Triphosphate
67 rdf:type schema:DefinedTerm
68 Nb413ff0812a24e909a834ffd958889af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Phosphates
70 rdf:type schema:DefinedTerm
71 Ne077d065acc847a8936ec2d15cb142f6 schema:name nlm_unique_id
72 schema:value 0410427
73 rdf:type schema:PropertyValue
74 Ne284918529014cf489d3555c159119df schema:issueNumber 3
75 rdf:type schema:PublicationIssue
76 Ne4fb795e678941dd92991831fa9a329e rdf:first sg:person.01126040223.41
77 rdf:rest N654489d0e28d44d99d495163204fcb94
78 Nf717f9ffb3ad406e9416e7d7d8e157c3 schema:name readcube_id
79 schema:value a6b9205c5e1acc8d26ea4316fd51bf9649867a89bd2f7128d0f5dd83275246c0
80 rdf:type schema:PropertyValue
81 Nfc2ac18625f34935a5eb4d86c1fc9282 schema:name doi
82 schema:value 10.1007/s002030050692
83 rdf:type schema:PropertyValue
84 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
85 schema:name Biological Sciences
86 rdf:type schema:DefinedTerm
87 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
88 schema:name Biochemistry and Cell Biology
89 rdf:type schema:DefinedTerm
90 sg:journal.1018945 schema:issn 0302-8933
91 1432-072X
92 schema:name Archives of Microbiology
93 rdf:type schema:Periodical
94 sg:person.01126040223.41 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
95 schema:familyName Petersen
96 schema:givenName Bent L.
97 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126040223.41
98 rdf:type schema:Person
99 sg:person.01156254575.47 schema:affiliation https://www.grid.ac/institutes/grid.418674.8
100 schema:familyName Kannangara
101 schema:givenName C. G.
102 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156254575.47
103 rdf:type schema:Person
104 sg:person.0717526447.08 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
105 schema:familyName Henningsen
106 schema:givenName Knud W.
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717526447.08
108 rdf:type schema:Person
109 https://www.grid.ac/institutes/grid.418674.8 schema:alternateName Carlsberg Laboratory
110 schema:name Department of Physiology, Carlsberg Laboratory, DK-2500 Valby, Copenhagen, Denmark e-mail: gamini@crc.dk Tel. +45-3327-5272; Fax +45-3327-4766, DK
111 rdf:type schema:Organization
112 https://www.grid.ac/institutes/grid.5254.6 schema:alternateName University of Copenhagen
113 schema:name Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark., DK
114 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...