Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-02

AUTHORS

Ulrich Dobrindt, M. Blaut

ABSTRACT

Hydrogenase was solubilized from the cytoplasmic membrane fraction of betaine-grown Sporomusa sphaeroides, and the enzyme was purified under oxic conditions. The oxygen-sensitive enzyme was partially reactivated under reducing conditions, resulting in a maximal activity of 19.8 μmol H2 oxidized min-1 (mg protein)-1 with benzyl viologen as electron acceptor and an apparent Km value for H2 of 341 μM. The molecular mass of the native protein estimated by native PAGE and gel filtration was 122 and 130 kDa, respectively. SDS-PAGE revealed two polypeptides with molecular masses of 65 and 37 kDa, present in a 1:1 ratio. The native protein contained 15.6 +/- 1.7 mol Fe, 11.4 +/- 1.4 mol S2-, and 0.6 mol Ni per mol enzyme. The hydrogenase coupled with viologen dyes, but not with other various artificial electron carriers, FAD, FMN, or NAD(P)+. The amino acid sequence of the N-termini of the subunits showed a high degree of similarity to eubacterial membrane-bound uptake hydrogenases. Washed membranes catalyzed a H2-dependent cytochrome b reduction at a rate of 0.18 nmol min-1 (mg protein)-1. More... »

PAGES

141-147

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002030050309

DOI

http://dx.doi.org/10.1007/s002030050309

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006357387

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8593101


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytochromes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electron Transport", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gram-Negative Anaerobic Bacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogenase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Weight", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidation-Reduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of G\u00f6ttingen", 
          "id": "https://www.grid.ac/institutes/grid.7450.6", 
          "name": [
            "Institut f\u00fcr Mikrobiologie der Universit\u00e4t G\u00f6ttingen,  Grisebachstr. 8, D-37077 G\u00f6ttingen, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dobrindt", 
        "givenName": "Ulrich", 
        "id": "sg:person.0601074636.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601074636.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of G\u00f6ttingen", 
          "id": "https://www.grid.ac/institutes/grid.7450.6", 
          "name": [
            "Institut f\u00fcr Mikrobiologie der Universit\u00e4t G\u00f6ttingen,  Grisebachstr. 8, D-37077 G\u00f6ttingen, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Blaut", 
        "givenName": "M.", 
        "id": "sg:person.01304611257.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304611257.43"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1996-02", 
    "datePublishedReg": "1996-02-01", 
    "description": "Hydrogenase was solubilized from the cytoplasmic membrane fraction of betaine-grown Sporomusa sphaeroides, and the enzyme was purified under oxic conditions. The oxygen-sensitive enzyme was partially reactivated under reducing conditions, resulting in a maximal activity of 19.8 \u03bcmol H2 oxidized min-1 (mg protein)-1 with benzyl viologen as electron acceptor and an apparent Km value for H2 of 341 \u03bcM. The molecular mass of the native protein estimated by native PAGE and gel filtration was 122 and 130 kDa, respectively. SDS-PAGE revealed two polypeptides with molecular masses of 65 and 37 kDa, present in a 1:1 ratio. The native protein contained 15.6 +/- 1.7 mol Fe, 11.4 +/- 1.4 mol S2-, and 0.6 mol Ni per mol enzyme. The hydrogenase coupled with viologen dyes, but not with other various artificial electron carriers, FAD, FMN, or NAD(P)+. The amino acid sequence of the N-termini of the subunits showed a high degree of similarity to eubacterial membrane-bound uptake hydrogenases. Washed membranes catalyzed a H2-dependent cytochrome b reduction at a rate of 0.18 nmol min-1 (mg protein)-1.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002030050309", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018945", 
        "issn": [
          "0302-8933", 
          "1432-072X"
        ], 
        "name": "Archives of Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "165"
      }
    ], 
    "name": "Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport", 
    "pagination": "141-147", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1e482e10ca7b532c14bd3c4d76cbf1ae87b0d6c57c980dd9d1d8a77b04ab545c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8593101"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410427"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002030050309"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1006357387"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002030050309", 
      "https://app.dimensions.ai/details/publication/pub.1006357387"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000486.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s002030050309"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002030050309'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002030050309'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002030050309'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002030050309'


 

This table displays all metadata directly associated to this object as RDF triples.

120 TRIPLES      20 PREDICATES      40 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002030050309 schema:about N05304d62da384812b1591fd8e62b2c78
2 N066ed5fab34f403c9fcd81c7de443546
3 N114eedc827e1451a8eb8bd040b1414a7
4 N25075c678f224bec9d00272f88b24bdb
5 N2fbc10076f2944f58f7564c5052be266
6 N5ac5198bcdd6459f9996da0452e75ab2
7 N5d1eee299962462f880c80f3e66375cc
8 N625c432880b5421ab716a6635a0f0391
9 N84069eb8a72d4e7f87951456709fe7bb
10 Nb3af554b18c143cfaa9a59d9bd3258d6
11 Ncf7ef3afe38f44ca930d53dceb520a3b
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author N05820c031e5041fd88d9baf717d05005
15 schema:datePublished 1996-02
16 schema:datePublishedReg 1996-02-01
17 schema:description Hydrogenase was solubilized from the cytoplasmic membrane fraction of betaine-grown Sporomusa sphaeroides, and the enzyme was purified under oxic conditions. The oxygen-sensitive enzyme was partially reactivated under reducing conditions, resulting in a maximal activity of 19.8 μmol H2 oxidized min-1 (mg protein)-1 with benzyl viologen as electron acceptor and an apparent Km value for H2 of 341 μM. The molecular mass of the native protein estimated by native PAGE and gel filtration was 122 and 130 kDa, respectively. SDS-PAGE revealed two polypeptides with molecular masses of 65 and 37 kDa, present in a 1:1 ratio. The native protein contained 15.6 +/- 1.7 mol Fe, 11.4 +/- 1.4 mol S2-, and 0.6 mol Ni per mol enzyme. The hydrogenase coupled with viologen dyes, but not with other various artificial electron carriers, FAD, FMN, or NAD(P)+. The amino acid sequence of the N-termini of the subunits showed a high degree of similarity to eubacterial membrane-bound uptake hydrogenases. Washed membranes catalyzed a H2-dependent cytochrome b reduction at a rate of 0.18 nmol min-1 (mg protein)-1.
18 schema:genre research_article
19 schema:inLanguage en
20 schema:isAccessibleForFree false
21 schema:isPartOf N02b8105f683b4af1944f26f26b057b62
22 N18caa324f61d4b17b67e56ce3e8a6c9f
23 sg:journal.1018945
24 schema:name Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport
25 schema:pagination 141-147
26 schema:productId N387034c2d4fb4d92b1cca3c742d31ce2
27 N38aa13028dc24ec78e78fa0c8be2f0e5
28 N9c94a730fdcf4151a5b563ca5e234ab7
29 Nb13d79fc64564a57b08a9f31b9e6e7b1
30 Nc257542714834b14945e360537a7fe82
31 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006357387
32 https://doi.org/10.1007/s002030050309
33 schema:sdDatePublished 2019-04-10T19:51
34 schema:sdLicense https://scigraph.springernature.com/explorer/license/
35 schema:sdPublisher Ne5e5e110b03e49398b6f0357dee6b815
36 schema:url http://link.springer.com/10.1007/s002030050309
37 sgo:license sg:explorer/license/
38 sgo:sdDataset articles
39 rdf:type schema:ScholarlyArticle
40 N02b8105f683b4af1944f26f26b057b62 schema:issueNumber 2
41 rdf:type schema:PublicationIssue
42 N05304d62da384812b1591fd8e62b2c78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
43 schema:name Oxidation-Reduction
44 rdf:type schema:DefinedTerm
45 N05820c031e5041fd88d9baf717d05005 rdf:first sg:person.0601074636.51
46 rdf:rest Nc7dda62ffbe54ac1a79e45cc6461749d
47 N066ed5fab34f403c9fcd81c7de443546 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Hydrogenase
49 rdf:type schema:DefinedTerm
50 N114eedc827e1451a8eb8bd040b1414a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Gram-Negative Anaerobic Bacteria
52 rdf:type schema:DefinedTerm
53 N18caa324f61d4b17b67e56ce3e8a6c9f schema:volumeNumber 165
54 rdf:type schema:PublicationVolume
55 N25075c678f224bec9d00272f88b24bdb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Hydrogen
57 rdf:type schema:DefinedTerm
58 N2fbc10076f2944f58f7564c5052be266 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name Sequence Homology, Amino Acid
60 rdf:type schema:DefinedTerm
61 N387034c2d4fb4d92b1cca3c742d31ce2 schema:name doi
62 schema:value 10.1007/s002030050309
63 rdf:type schema:PropertyValue
64 N38aa13028dc24ec78e78fa0c8be2f0e5 schema:name pubmed_id
65 schema:value 8593101
66 rdf:type schema:PropertyValue
67 N5ac5198bcdd6459f9996da0452e75ab2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Cytochromes
69 rdf:type schema:DefinedTerm
70 N5d1eee299962462f880c80f3e66375cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Amino Acid Sequence
72 rdf:type schema:DefinedTerm
73 N625c432880b5421ab716a6635a0f0391 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Molecular Sequence Data
75 rdf:type schema:DefinedTerm
76 N84069eb8a72d4e7f87951456709fe7bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Cell Membrane
78 rdf:type schema:DefinedTerm
79 N9c94a730fdcf4151a5b563ca5e234ab7 schema:name nlm_unique_id
80 schema:value 0410427
81 rdf:type schema:PropertyValue
82 Nb13d79fc64564a57b08a9f31b9e6e7b1 schema:name dimensions_id
83 schema:value pub.1006357387
84 rdf:type schema:PropertyValue
85 Nb3af554b18c143cfaa9a59d9bd3258d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Electron Transport
87 rdf:type schema:DefinedTerm
88 Nc257542714834b14945e360537a7fe82 schema:name readcube_id
89 schema:value 1e482e10ca7b532c14bd3c4d76cbf1ae87b0d6c57c980dd9d1d8a77b04ab545c
90 rdf:type schema:PropertyValue
91 Nc7dda62ffbe54ac1a79e45cc6461749d rdf:first sg:person.01304611257.43
92 rdf:rest rdf:nil
93 Ncf7ef3afe38f44ca930d53dceb520a3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Molecular Weight
95 rdf:type schema:DefinedTerm
96 Ne5e5e110b03e49398b6f0357dee6b815 schema:name Springer Nature - SN SciGraph project
97 rdf:type schema:Organization
98 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
99 schema:name Biological Sciences
100 rdf:type schema:DefinedTerm
101 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
102 schema:name Biochemistry and Cell Biology
103 rdf:type schema:DefinedTerm
104 sg:journal.1018945 schema:issn 0302-8933
105 1432-072X
106 schema:name Archives of Microbiology
107 rdf:type schema:Periodical
108 sg:person.01304611257.43 schema:affiliation https://www.grid.ac/institutes/grid.7450.6
109 schema:familyName Blaut
110 schema:givenName M.
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304611257.43
112 rdf:type schema:Person
113 sg:person.0601074636.51 schema:affiliation https://www.grid.ac/institutes/grid.7450.6
114 schema:familyName Dobrindt
115 schema:givenName Ulrich
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601074636.51
117 rdf:type schema:Person
118 https://www.grid.ac/institutes/grid.7450.6 schema:alternateName University of Göttingen
119 schema:name Institut für Mikrobiologie der Universität Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany, DE
120 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...