Influence of the fusion of two subunits of the F420-non-reducing hydrogenase of Methanococcus voltae on its biochemical properties View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-11

AUTHORS

Ruth Bingemann, Antonio J. Pierik, Albrecht Klein

ABSTRACT

In Methanococcus voltae, one of the two [NiFeSe] hydrogenases is unusual in that the large subunit is split into two subunits, each contributing two ligands to the [NiFe] center that catalyzes the heterolytic cleavage of the dihydrogen molecule. We have engineered a fusion of these two subunits. The resulting new enzyme showed no significant difference in hydrogen uptake activity or in the Ni-C or Ni-L EPR spectra compared to the the wild-type enzyme, but exhibited a tenfold increase in both the Km for hydrogen and the Ki for the competitive inhibitor carbon monoxide. More... »

PAGES

375-378

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002030000213

DOI

http://dx.doi.org/10.1007/s002030000213

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014801253

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11131028


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electron Spin Resonance Spectroscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogenase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methanococcus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidoreductases", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Philipps-Universit\u00e4t Marburg, Fachbereich Biologie, Molekulargenetik, Karl-von-Frisch-Strasse, 35032 Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bingemann", 
        "givenName": "Ruth", 
        "id": "sg:person.01016145546.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016145546.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Philipps-Universit\u00e4t Marburg, Fachbereich Biologie, Mikrobiologie, Karl-von-Frisch-Strasse, 35032 Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pierik", 
        "givenName": "Antonio J.", 
        "id": "sg:person.01322425302.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322425302.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Philipps-Universit\u00e4t Marburg, Fachbereich Biologie, Molekulargenetik, Karl-von-Frisch-Strasse, 35032 Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klein", 
        "givenName": "Albrecht", 
        "id": "sg:person.014554511742.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014554511742.38"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2000-11", 
    "datePublishedReg": "2000-11-01", 
    "description": "In Methanococcus voltae, one of the two [NiFeSe] hydrogenases is unusual in that the large subunit is split into two subunits, each contributing two ligands to the [NiFe] center that catalyzes the heterolytic cleavage of the dihydrogen molecule. We have engineered a fusion of these two subunits. The resulting new enzyme showed no significant difference in hydrogen uptake activity or in the Ni-C or Ni-L EPR spectra compared to the the wild-type enzyme, but exhibited a tenfold increase in both the Km for hydrogen and the Ki for the competitive inhibitor carbon monoxide.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002030000213", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018945", 
        "issn": [
          "0302-8933", 
          "1432-072X"
        ], 
        "name": "Archives of Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "174"
      }
    ], 
    "name": "Influence of the fusion of two subunits of the F420-non-reducing hydrogenase of Methanococcus voltae on its biochemical properties", 
    "pagination": "375-378", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "46844b50992b689aa82cb0864e44f73e55ad186aee457127ad8989efb08ea72b"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11131028"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410427"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002030000213"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1014801253"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002030000213", 
      "https://app.dimensions.ai/details/publication/pub.1014801253"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000480.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s002030000213"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002030000213'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002030000213'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002030000213'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002030000213'


 

This table displays all metadata directly associated to this object as RDF triples.

112 TRIPLES      20 PREDICATES      36 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002030000213 schema:about N123e42df558a4d219155e7f7501e0c26
2 N171ece052a1e415d98221160a5d5c89a
3 N19d8bbba32144b8097407b22f66dd227
4 N25188786d9934cceb9a0797d7c232c15
5 N44fa67413d054bdf932352b1a312feaa
6 N882f5e7bdcba43bf95276799b49a242e
7 N9b51489f124340e193d2b02308372ed4
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author Nacd6cc11b68248b9827af1a7a82bd5fd
11 schema:datePublished 2000-11
12 schema:datePublishedReg 2000-11-01
13 schema:description In Methanococcus voltae, one of the two [NiFeSe] hydrogenases is unusual in that the large subunit is split into two subunits, each contributing two ligands to the [NiFe] center that catalyzes the heterolytic cleavage of the dihydrogen molecule. We have engineered a fusion of these two subunits. The resulting new enzyme showed no significant difference in hydrogen uptake activity or in the Ni-C or Ni-L EPR spectra compared to the the wild-type enzyme, but exhibited a tenfold increase in both the Km for hydrogen and the Ki for the competitive inhibitor carbon monoxide.
14 schema:genre research_article
15 schema:inLanguage en
16 schema:isAccessibleForFree false
17 schema:isPartOf N3805b4135a6c43ab8733716510a0748f
18 N5e9e2b61a06a4052a399c7dafe12acf0
19 sg:journal.1018945
20 schema:name Influence of the fusion of two subunits of the F420-non-reducing hydrogenase of Methanococcus voltae on its biochemical properties
21 schema:pagination 375-378
22 schema:productId N07a9ca2b59bc4bd19289c45a35e3badf
23 N0c27f324c4bf4054938bf0b6dc613033
24 Nb395daba81a041adb06b84dd34c9bd73
25 Nead4eaf1beec4d65ad42b8c57cec80aa
26 Nf5e9ad78a09448f5b7179d7a1ed4c5ec
27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014801253
28 https://doi.org/10.1007/s002030000213
29 schema:sdDatePublished 2019-04-10T14:52
30 schema:sdLicense https://scigraph.springernature.com/explorer/license/
31 schema:sdPublisher N9a01ade70e05476a8c5c0c1b19953e4f
32 schema:url http://link.springer.com/10.1007/s002030000213
33 sgo:license sg:explorer/license/
34 sgo:sdDataset articles
35 rdf:type schema:ScholarlyArticle
36 N07a9ca2b59bc4bd19289c45a35e3badf schema:name pubmed_id
37 schema:value 11131028
38 rdf:type schema:PropertyValue
39 N0b346933b3c64d77a494946320ac4efe rdf:first sg:person.01322425302.19
40 rdf:rest N127d2810b2aa41c7b3b802598019c12d
41 N0c27f324c4bf4054938bf0b6dc613033 schema:name readcube_id
42 schema:value 46844b50992b689aa82cb0864e44f73e55ad186aee457127ad8989efb08ea72b
43 rdf:type schema:PropertyValue
44 N123e42df558a4d219155e7f7501e0c26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
45 schema:name Kinetics
46 rdf:type schema:DefinedTerm
47 N127d2810b2aa41c7b3b802598019c12d rdf:first sg:person.014554511742.38
48 rdf:rest rdf:nil
49 N171ece052a1e415d98221160a5d5c89a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name Hydrogen
51 rdf:type schema:DefinedTerm
52 N19d8bbba32144b8097407b22f66dd227 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Hydrogenase
54 rdf:type schema:DefinedTerm
55 N25188786d9934cceb9a0797d7c232c15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Binding Sites
57 rdf:type schema:DefinedTerm
58 N3805b4135a6c43ab8733716510a0748f schema:issueNumber 5
59 rdf:type schema:PublicationIssue
60 N44fa67413d054bdf932352b1a312feaa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Electron Spin Resonance Spectroscopy
62 rdf:type schema:DefinedTerm
63 N5e9e2b61a06a4052a399c7dafe12acf0 schema:volumeNumber 174
64 rdf:type schema:PublicationVolume
65 N882f5e7bdcba43bf95276799b49a242e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Methanococcus
67 rdf:type schema:DefinedTerm
68 N9a01ade70e05476a8c5c0c1b19953e4f schema:name Springer Nature - SN SciGraph project
69 rdf:type schema:Organization
70 N9b51489f124340e193d2b02308372ed4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Oxidoreductases
72 rdf:type schema:DefinedTerm
73 Nacd6cc11b68248b9827af1a7a82bd5fd rdf:first sg:person.01016145546.74
74 rdf:rest N0b346933b3c64d77a494946320ac4efe
75 Nb395daba81a041adb06b84dd34c9bd73 schema:name dimensions_id
76 schema:value pub.1014801253
77 rdf:type schema:PropertyValue
78 Nead4eaf1beec4d65ad42b8c57cec80aa schema:name nlm_unique_id
79 schema:value 0410427
80 rdf:type schema:PropertyValue
81 Nf5e9ad78a09448f5b7179d7a1ed4c5ec schema:name doi
82 schema:value 10.1007/s002030000213
83 rdf:type schema:PropertyValue
84 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
85 schema:name Biological Sciences
86 rdf:type schema:DefinedTerm
87 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
88 schema:name Biochemistry and Cell Biology
89 rdf:type schema:DefinedTerm
90 sg:journal.1018945 schema:issn 0302-8933
91 1432-072X
92 schema:name Archives of Microbiology
93 rdf:type schema:Periodical
94 sg:person.01016145546.74 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
95 schema:familyName Bingemann
96 schema:givenName Ruth
97 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016145546.74
98 rdf:type schema:Person
99 sg:person.01322425302.19 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
100 schema:familyName Pierik
101 schema:givenName Antonio J.
102 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322425302.19
103 rdf:type schema:Person
104 sg:person.014554511742.38 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
105 schema:familyName Klein
106 schema:givenName Albrecht
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014554511742.38
108 rdf:type schema:Person
109 https://www.grid.ac/institutes/grid.10253.35 schema:alternateName Philipp University of Marburg
110 schema:name Philipps-Universität Marburg, Fachbereich Biologie, Mikrobiologie, Karl-von-Frisch-Strasse, 35032 Marburg, Germany
111 Philipps-Universität Marburg, Fachbereich Biologie, Molekulargenetik, Karl-von-Frisch-Strasse, 35032 Marburg, Germany
112 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...