Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-04-05

AUTHORS

L. Verdiguel-Fernández, R. Oropeza-Navarro, Francisco J. Basurto-Alcántara, A. Castañeda-Ramírez, Antonio Verdugo-Rodríguez

ABSTRACT

Brucellosis is an infectious disease that affects practically all species of mammals, including human, and is a major zoonosis worldwide. Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in phagocytic and nonphagocytic cells such as trophoblast and epithelial cells. Among the six recognized species of the genus Brucella, Brucella melitensis is the main etiological agent involved in goat brucellosis and is also the most pathogenic for human. It causes significant losses in livestock production as a result of abortions, metritis, infertility, and birth of weak animals. Outer membrane proteins (OMPs) are exposed on the bacterial surface and are in contact with cells and effectors of the host immune response, whereby they could be important virulence factors of Brucella species. To evaluate this hypothesis, the gene encoding for the major outer membrane protein Omp31 was amplified, cloned into pUC18 plasmid, and inactivated by inserting a kanamycin cassette, rendering pLVM31 plasmid which was transformed into B. melitensis wild-type strain to obtain LVM31 mutant strain. The Outer membrane (OM) properties of the mutant strain were compared with B. melitensis Bm133 wild-type and B. melitensis Rev1 vaccine strains, in assessing its susceptibility to polymyxin B, sodium deoxycholate, and nonimmune serum. The mutant strain was assessed in vitro with survival assays in murine macrophages J774.A1 and HeLa cells. Our results demonstrate that LVM31 mutant is more susceptible to polymyxin B, sodium deoxycholate, and nonimmune serum than control strains; moreover, Omp31 mutation caused a decrease in the internalization and a significant decrease in the intracellular survival compared with the reference strains in both cell lines. These results allow us to conclude that Omp31 is important for maintaining OM integrity, but also it is necessary for bacterial internalization, establishment and development of an optimal replication niche, and essential for survival and intracellular multiplication. More... »

PAGES

971-978

References to SciGraph publications

  • 2012-03-31. Progress in Brucella vaccine development in FRONTIERS IN BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00203-017-1360-7

    DOI

    http://dx.doi.org/10.1007/s00203-017-1360-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1084517364

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28382472


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Outer Membrane Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Brucella melitensis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Brucellosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Deoxycholic Acid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HeLa Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Macrophages", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microbial Sensitivity Tests", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymyxin B", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Virulence Factors", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Laboratorio de Microbiolog\u00eda Molecular, Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, colonia UNAM CU, C.P 04510, Coyoac\u00e1n, CdMx, Mexico", 
              "id": "http://www.grid.ac/institutes/grid.9486.3", 
              "name": [
                "Laboratorio de Microbiolog\u00eda Molecular, Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, colonia UNAM CU, C.P 04510, Coyoac\u00e1n, CdMx, Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Verdiguel-Fern\u00e1ndez", 
            "givenName": "L.", 
            "id": "sg:person.013124205013.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013124205013.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Departamento de Microbiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernvaca, Morelos, Mexico", 
              "id": "http://www.grid.ac/institutes/grid.9486.3", 
              "name": [
                "Departamento de Microbiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernvaca, Morelos, Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Oropeza-Navarro", 
            "givenName": "R.", 
            "id": "sg:person.0653012252.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653012252.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratorio de Vacunolog\u00eda, Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Coyoac\u00e1n, CdMx, Mexico", 
              "id": "http://www.grid.ac/institutes/grid.9486.3", 
              "name": [
                "Laboratorio de Vacunolog\u00eda, Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Coyoac\u00e1n, CdMx, Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Basurto-Alc\u00e1ntara", 
            "givenName": "Francisco J.", 
            "id": "sg:person.010754063653.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010754063653.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Departamento de Zootecnia, Universidad Aut\u00f3noma de Chapingo, Texcoco, Mexico", 
              "id": "http://www.grid.ac/institutes/grid.34684.3d", 
              "name": [
                "Departamento de Zootecnia, Universidad Aut\u00f3noma de Chapingo, Texcoco, Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Casta\u00f1eda-Ram\u00edrez", 
            "givenName": "A.", 
            "id": "sg:person.01235526410.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235526410.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratorio de Microbiolog\u00eda Molecular, Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, colonia UNAM CU, C.P 04510, Coyoac\u00e1n, CdMx, Mexico", 
              "id": "http://www.grid.ac/institutes/grid.9486.3", 
              "name": [
                "Laboratorio de Microbiolog\u00eda Molecular, Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, colonia UNAM CU, C.P 04510, Coyoac\u00e1n, CdMx, Mexico"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Verdugo-Rodr\u00edguez", 
            "givenName": "Antonio", 
            "id": "sg:person.01130222726.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130222726.06"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s11515-012-1196-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032086951", 
              "https://doi.org/10.1007/s11515-012-1196-0"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-04-05", 
        "datePublishedReg": "2017-04-05", 
        "description": "Brucellosis is an infectious disease that affects practically all species of mammals, including human, and is a major zoonosis worldwide. Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in phagocytic and nonphagocytic cells such as trophoblast and epithelial cells. Among the six recognized species of the genus Brucella, Brucella melitensis is the main etiological agent involved in goat brucellosis and is also the most pathogenic for human. It causes significant losses in livestock production as a result of abortions, metritis, infertility, and birth of weak animals. Outer membrane proteins (OMPs) are exposed on the bacterial surface and are in contact with cells and effectors of the host immune response, whereby they could be important virulence factors of Brucella species. To evaluate this hypothesis, the gene encoding for the major outer membrane protein Omp31 was amplified, cloned into pUC18 plasmid, and inactivated by inserting a kanamycin cassette, rendering pLVM31 plasmid which was transformed into B. melitensis wild-type strain to obtain LVM31 mutant strain. The Outer membrane (OM) properties of the mutant strain were compared with B. melitensis Bm133 wild-type and B. melitensis Rev1 vaccine strains, in assessing its susceptibility to polymyxin B, sodium deoxycholate, and nonimmune serum. The mutant strain was assessed in vitro with survival assays in murine macrophages J774.A1 and HeLa cells. Our results demonstrate that LVM31 mutant is more susceptible to polymyxin B, sodium deoxycholate, and nonimmune serum than control strains; moreover, Omp31 mutation caused a decrease in the internalization and a significant decrease in the intracellular survival compared with the reference strains in both cell lines. These results allow us to conclude that Omp31 is important for maintaining OM integrity, but also it is necessary for bacterial internalization, establishment and development of an optimal replication niche, and essential for survival and intracellular multiplication.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s00203-017-1360-7", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018945", 
            "issn": [
              "0302-8933", 
              "1432-072X"
            ], 
            "name": "Archives of Microbiology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "199"
          }
        ], 
        "keywords": [
          "outer membrane properties", 
          "outer membrane proteins", 
          "mutant strain", 
          "intracellular survival", 
          "HeLa cells", 
          "species of mammals", 
          "wild-type strain", 
          "facultative intracellular pathogen", 
          "kanamycin cassette", 
          "OM integrity", 
          "membrane proteins", 
          "important virulence factor", 
          "replication niche", 
          "bacterial internalization", 
          "nonphagocytic cells", 
          "genus Brucella", 
          "intracellular pathogens", 
          "nonimmune serum", 
          "Brucella species", 
          "virulence factors", 
          "result of abortion", 
          "Brucella melitensis", 
          "bacterial surface", 
          "survival assays", 
          "intracellular multiplication", 
          "species", 
          "murine macrophages", 
          "cell lines", 
          "pUC18 plasmid", 
          "host immune response", 
          "epithelial cells", 
          "main etiological agent", 
          "Omp31", 
          "plasmid", 
          "sodium deoxycholate", 
          "cells", 
          "weak animals", 
          "membrane properties", 
          "immune response", 
          "internalization", 
          "etiological agent", 
          "livestock production", 
          "infectious diseases", 
          "strains", 
          "polymyxin B", 
          "important role", 
          "mutants", 
          "major zoonosis", 
          "significant decrease", 
          "mammals", 
          "genes", 
          "niche", 
          "survival", 
          "melitensis", 
          "effectors", 
          "protein", 
          "cassette", 
          "mutations", 
          "serum", 
          "brucellosis", 
          "goat brucellosis", 
          "pathogens", 
          "deoxycholate", 
          "Brucella", 
          "assays", 
          "infertility", 
          "vaccine", 
          "disease", 
          "trophoblast", 
          "macrophages", 
          "significant loss", 
          "animals", 
          "establishment", 
          "birth", 
          "humans", 
          "zoonosis", 
          "abortion", 
          "role", 
          "decrease", 
          "integrity", 
          "metritis", 
          "production", 
          "lines", 
          "susceptibility", 
          "hypothesis", 
          "response", 
          "agents", 
          "ability", 
          "loss", 
          "development", 
          "factors", 
          "control", 
          "multiplication", 
          "results", 
          "contact", 
          "properties", 
          "surface", 
          "reference"
        ], 
        "name": "Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells", 
        "pagination": "971-978", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1084517364"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00203-017-1360-7"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28382472"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00203-017-1360-7", 
          "https://app.dimensions.ai/details/publication/pub.1084517364"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T21:01", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_741.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s00203-017-1360-7"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00203-017-1360-7'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00203-017-1360-7'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00203-017-1360-7'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00203-017-1360-7'


     

    This table displays all metadata directly associated to this object as RDF triples.

    270 TRIPLES      21 PREDICATES      142 URIs      130 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00203-017-1360-7 schema:about N031229fa1a17407d8f31c44000b5ea52
    2 N12f35dc81c39485cbd84cfa4a63084ac
    3 N1ddc9a3e0c8e4103b7f6352bab21df2a
    4 N3854656b46384c4c85598a9ef3f3965b
    5 N442cc96580d842009b80ad399a628148
    6 N6828d3b574e24aca8e7542ff00fdebcb
    7 N75ec0dc951d54b2384d1fbef057a79f1
    8 Nb007329f6ad1441e93ae441a190560b8
    9 Nb76f5f3a84e14fd3b0747ace62a8d0ba
    10 Nb83db1cd7780463da6c91d22d2040f37
    11 Nbeb8b2cc66d243f0afb4c46ce06e7f3d
    12 Ndeadc7266c8e41328486e069280a2d4f
    13 Ne4d16c0c9eff4b8f99cfe8a7943af24c
    14 Nf4ed64f0c70345ec9db2fe924e902092
    15 Nf8200eb6c3394009862cee97209f7e2b
    16 anzsrc-for:06
    17 anzsrc-for:0601
    18 anzsrc-for:0605
    19 anzsrc-for:11
    20 anzsrc-for:1108
    21 schema:author N740c5427c79c482db36627e80c308eac
    22 schema:citation sg:pub.10.1007/s11515-012-1196-0
    23 schema:datePublished 2017-04-05
    24 schema:datePublishedReg 2017-04-05
    25 schema:description Brucellosis is an infectious disease that affects practically all species of mammals, including human, and is a major zoonosis worldwide. Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in phagocytic and nonphagocytic cells such as trophoblast and epithelial cells. Among the six recognized species of the genus Brucella, Brucella melitensis is the main etiological agent involved in goat brucellosis and is also the most pathogenic for human. It causes significant losses in livestock production as a result of abortions, metritis, infertility, and birth of weak animals. Outer membrane proteins (OMPs) are exposed on the bacterial surface and are in contact with cells and effectors of the host immune response, whereby they could be important virulence factors of Brucella species. To evaluate this hypothesis, the gene encoding for the major outer membrane protein Omp31 was amplified, cloned into pUC18 plasmid, and inactivated by inserting a kanamycin cassette, rendering pLVM31 plasmid which was transformed into B. melitensis wild-type strain to obtain LVM31 mutant strain. The Outer membrane (OM) properties of the mutant strain were compared with B. melitensis Bm133 wild-type and B. melitensis Rev1 vaccine strains, in assessing its susceptibility to polymyxin B, sodium deoxycholate, and nonimmune serum. The mutant strain was assessed in vitro with survival assays in murine macrophages J774.A1 and HeLa cells. Our results demonstrate that LVM31 mutant is more susceptible to polymyxin B, sodium deoxycholate, and nonimmune serum than control strains; moreover, Omp31 mutation caused a decrease in the internalization and a significant decrease in the intracellular survival compared with the reference strains in both cell lines. These results allow us to conclude that Omp31 is important for maintaining OM integrity, but also it is necessary for bacterial internalization, establishment and development of an optimal replication niche, and essential for survival and intracellular multiplication.
    26 schema:genre article
    27 schema:isAccessibleForFree false
    28 schema:isPartOf N7956bb843aba4b51ad8d74fe541b671c
    29 Nb75ef5d7043a4cd9b36b362fe20e53e7
    30 sg:journal.1018945
    31 schema:keywords Brucella
    32 Brucella melitensis
    33 Brucella species
    34 HeLa cells
    35 OM integrity
    36 Omp31
    37 ability
    38 abortion
    39 agents
    40 animals
    41 assays
    42 bacterial internalization
    43 bacterial surface
    44 birth
    45 brucellosis
    46 cassette
    47 cell lines
    48 cells
    49 contact
    50 control
    51 decrease
    52 deoxycholate
    53 development
    54 disease
    55 effectors
    56 epithelial cells
    57 establishment
    58 etiological agent
    59 factors
    60 facultative intracellular pathogen
    61 genes
    62 genus Brucella
    63 goat brucellosis
    64 host immune response
    65 humans
    66 hypothesis
    67 immune response
    68 important role
    69 important virulence factor
    70 infectious diseases
    71 infertility
    72 integrity
    73 internalization
    74 intracellular multiplication
    75 intracellular pathogens
    76 intracellular survival
    77 kanamycin cassette
    78 lines
    79 livestock production
    80 loss
    81 macrophages
    82 main etiological agent
    83 major zoonosis
    84 mammals
    85 melitensis
    86 membrane properties
    87 membrane proteins
    88 metritis
    89 multiplication
    90 murine macrophages
    91 mutant strain
    92 mutants
    93 mutations
    94 niche
    95 nonimmune serum
    96 nonphagocytic cells
    97 outer membrane properties
    98 outer membrane proteins
    99 pUC18 plasmid
    100 pathogens
    101 plasmid
    102 polymyxin B
    103 production
    104 properties
    105 protein
    106 reference
    107 replication niche
    108 response
    109 result of abortion
    110 results
    111 role
    112 serum
    113 significant decrease
    114 significant loss
    115 sodium deoxycholate
    116 species
    117 species of mammals
    118 strains
    119 surface
    120 survival
    121 survival assays
    122 susceptibility
    123 trophoblast
    124 vaccine
    125 virulence factors
    126 weak animals
    127 wild-type strain
    128 zoonosis
    129 schema:name Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells
    130 schema:pagination 971-978
    131 schema:productId N9b3fa1fff03c4d88a6412405ea0b1f03
    132 Nc243b2aae4b94fe588ffaa8a3993694d
    133 Ne490d7daee0c44e593ca43aefc50412d
    134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084517364
    135 https://doi.org/10.1007/s00203-017-1360-7
    136 schema:sdDatePublished 2022-11-24T21:01
    137 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    138 schema:sdPublisher N023034d64a584cf0bdb6809486385c55
    139 schema:url https://doi.org/10.1007/s00203-017-1360-7
    140 sgo:license sg:explorer/license/
    141 sgo:sdDataset articles
    142 rdf:type schema:ScholarlyArticle
    143 N023034d64a584cf0bdb6809486385c55 schema:name Springer Nature - SN SciGraph project
    144 rdf:type schema:Organization
    145 N031229fa1a17407d8f31c44000b5ea52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Brucella melitensis
    147 rdf:type schema:DefinedTerm
    148 N12f35dc81c39485cbd84cfa4a63084ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Mutation
    150 rdf:type schema:DefinedTerm
    151 N1ddc9a3e0c8e4103b7f6352bab21df2a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Cell Line, Tumor
    153 rdf:type schema:DefinedTerm
    154 N35cb0a508a3a4183807550efd39e37d4 rdf:first sg:person.0653012252.23
    155 rdf:rest N75e1a8060e2943adb2ea644dc302bd7e
    156 N3854656b46384c4c85598a9ef3f3965b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    157 schema:name Mice
    158 rdf:type schema:DefinedTerm
    159 N442cc96580d842009b80ad399a628148 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Microbial Sensitivity Tests
    161 rdf:type schema:DefinedTerm
    162 N6828d3b574e24aca8e7542ff00fdebcb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Brucellosis
    164 rdf:type schema:DefinedTerm
    165 N7408ae1f2fe94b248e6f86cc480ca45a rdf:first sg:person.01235526410.57
    166 rdf:rest Ne60ee550d5bb47de8d09419a7ea161aa
    167 N740c5427c79c482db36627e80c308eac rdf:first sg:person.013124205013.13
    168 rdf:rest N35cb0a508a3a4183807550efd39e37d4
    169 N75e1a8060e2943adb2ea644dc302bd7e rdf:first sg:person.010754063653.17
    170 rdf:rest N7408ae1f2fe94b248e6f86cc480ca45a
    171 N75ec0dc951d54b2384d1fbef057a79f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Humans
    173 rdf:type schema:DefinedTerm
    174 N7956bb843aba4b51ad8d74fe541b671c schema:issueNumber 7
    175 rdf:type schema:PublicationIssue
    176 N9b3fa1fff03c4d88a6412405ea0b1f03 schema:name doi
    177 schema:value 10.1007/s00203-017-1360-7
    178 rdf:type schema:PropertyValue
    179 Nb007329f6ad1441e93ae441a190560b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Bacterial Outer Membrane Proteins
    181 rdf:type schema:DefinedTerm
    182 Nb75ef5d7043a4cd9b36b362fe20e53e7 schema:volumeNumber 199
    183 rdf:type schema:PublicationVolume
    184 Nb76f5f3a84e14fd3b0747ace62a8d0ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Virulence Factors
    186 rdf:type schema:DefinedTerm
    187 Nb83db1cd7780463da6c91d22d2040f37 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name HeLa Cells
    189 rdf:type schema:DefinedTerm
    190 Nbeb8b2cc66d243f0afb4c46ce06e7f3d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    191 schema:name Animals
    192 rdf:type schema:DefinedTerm
    193 Nc243b2aae4b94fe588ffaa8a3993694d schema:name dimensions_id
    194 schema:value pub.1084517364
    195 rdf:type schema:PropertyValue
    196 Ndeadc7266c8e41328486e069280a2d4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    197 schema:name Deoxycholic Acid
    198 rdf:type schema:DefinedTerm
    199 Ne490d7daee0c44e593ca43aefc50412d schema:name pubmed_id
    200 schema:value 28382472
    201 rdf:type schema:PropertyValue
    202 Ne4d16c0c9eff4b8f99cfe8a7943af24c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    203 schema:name Polymyxin B
    204 rdf:type schema:DefinedTerm
    205 Ne60ee550d5bb47de8d09419a7ea161aa rdf:first sg:person.01130222726.06
    206 rdf:rest rdf:nil
    207 Nf4ed64f0c70345ec9db2fe924e902092 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    208 schema:name Plasmids
    209 rdf:type schema:DefinedTerm
    210 Nf8200eb6c3394009862cee97209f7e2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    211 schema:name Macrophages
    212 rdf:type schema:DefinedTerm
    213 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    214 schema:name Biological Sciences
    215 rdf:type schema:DefinedTerm
    216 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    217 schema:name Biochemistry and Cell Biology
    218 rdf:type schema:DefinedTerm
    219 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    220 schema:name Microbiology
    221 rdf:type schema:DefinedTerm
    222 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    223 schema:name Medical and Health Sciences
    224 rdf:type schema:DefinedTerm
    225 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    226 schema:name Medical Microbiology
    227 rdf:type schema:DefinedTerm
    228 sg:journal.1018945 schema:issn 0302-8933
    229 1432-072X
    230 schema:name Archives of Microbiology
    231 schema:publisher Springer Nature
    232 rdf:type schema:Periodical
    233 sg:person.010754063653.17 schema:affiliation grid-institutes:grid.9486.3
    234 schema:familyName Basurto-Alcántara
    235 schema:givenName Francisco J.
    236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010754063653.17
    237 rdf:type schema:Person
    238 sg:person.01130222726.06 schema:affiliation grid-institutes:grid.9486.3
    239 schema:familyName Verdugo-Rodríguez
    240 schema:givenName Antonio
    241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130222726.06
    242 rdf:type schema:Person
    243 sg:person.01235526410.57 schema:affiliation grid-institutes:grid.34684.3d
    244 schema:familyName Castañeda-Ramírez
    245 schema:givenName A.
    246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235526410.57
    247 rdf:type schema:Person
    248 sg:person.013124205013.13 schema:affiliation grid-institutes:grid.9486.3
    249 schema:familyName Verdiguel-Fernández
    250 schema:givenName L.
    251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013124205013.13
    252 rdf:type schema:Person
    253 sg:person.0653012252.23 schema:affiliation grid-institutes:grid.9486.3
    254 schema:familyName Oropeza-Navarro
    255 schema:givenName R.
    256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653012252.23
    257 rdf:type schema:Person
    258 sg:pub.10.1007/s11515-012-1196-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032086951
    259 https://doi.org/10.1007/s11515-012-1196-0
    260 rdf:type schema:CreativeWork
    261 grid-institutes:grid.34684.3d schema:alternateName Departamento de Zootecnia, Universidad Autónoma de Chapingo, Texcoco, Mexico
    262 schema:name Departamento de Zootecnia, Universidad Autónoma de Chapingo, Texcoco, Mexico
    263 rdf:type schema:Organization
    264 grid-institutes:grid.9486.3 schema:alternateName Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernvaca, Morelos, Mexico
    265 Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Avenida Universidad 3000, colonia UNAM CU, C.P 04510, Coyoacán, CdMx, Mexico
    266 Laboratorio de Vacunología, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, CdMx, Mexico
    267 schema:name Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernvaca, Morelos, Mexico
    268 Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Avenida Universidad 3000, colonia UNAM CU, C.P 04510, Coyoacán, CdMx, Mexico
    269 Laboratorio de Vacunología, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, CdMx, Mexico
    270 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...