Ontology type: schema:ScholarlyArticle Open Access: True
2010-10
AUTHORSSylvia M. C. Alquéres, Jose Henrique M. Oliveira, Eduardo M. Nogueira, Helma V. Guedes, Pedro L. Oliveira, Fernando Câmara, Jose I. Baldani, Orlando B. Martins
ABSTRACTGluconacetobacter diazotrophicus, an endophyte isolated from sugarcane, is a strict aerobe that fixates N(2). This process is catalyzed by nitrogenase and requires copious amounts of ATP. Nitrogenase activity is extremely sensitive to inhibition by oxygen and reactive oxygen species (ROS). However, the elevated oxidative metabolic rates required to sustain biological nitrogen fixation (BNF) may favor an increased production of ROS. Here, we explored this paradox and observed that ROS levels are, in fact, decreased in nitrogen-fixing cells due to the up-regulation of transcript levels of six ROS-detoxifying genes. A cluster analyses based on common expression patterns revealed the existence of a stable cluster with 99.8% similarity made up of the genes encoding the α-subunit of nitrogenase Mo-Fe protein (nifD), superoxide dismutase (sodA) and catalase type E (katE). Finally, nitrogenase activity was inhibited in a dose-dependent manner by paraquat, a redox cycler that increases cellular ROS levels. Our data revealed that ROS can strongly inhibit nitrogenase activity, and G. diazotrophicus alters its redox metabolism during BNF by increasing antioxidant transcript levels resulting in a lower ROS generation. We suggest that careful controlled ROS production during this critical phase is an adaptive mechanism to allow nitrogen fixation. More... »
PAGES835-841
http://scigraph.springernature.com/pub.10.1007/s00203-010-0609-1
DOIhttp://dx.doi.org/10.1007/s00203-010-0609-1
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1051258849
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/20697694
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antioxidants",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cluster Analysis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, Bacterial",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gluconacetobacter",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nitrogen Fixation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nitrogenase",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Paraquat",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Reactive Oxygen Species",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Up-Regulation",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Federal University of Rio de Janeiro",
"id": "https://www.grid.ac/institutes/grid.8536.8",
"name": [
"Laborat\u00f3rio de Biologia Molecular, Instituto de Bioqu\u00edmica M\u00e9dica, Universidade Federal do Rio de Janeiro, CEP 21941-590, Rio de Janeiro, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Alqu\u00e9res",
"givenName": "Sylvia M. C.",
"id": "sg:person.01073306112.87",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073306112.87"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Federal University of Rio de Janeiro",
"id": "https://www.grid.ac/institutes/grid.8536.8",
"name": [
"Laborat\u00f3rio de Biologia Molecular, Instituto de Bioqu\u00edmica M\u00e9dica, Universidade Federal do Rio de Janeiro, CEP 21941-590, Rio de Janeiro, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Oliveira",
"givenName": "Jose Henrique M.",
"id": "sg:person.0611547237.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611547237.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Federal University of the State of Rio de Janeiro",
"id": "https://www.grid.ac/institutes/grid.467095.9",
"name": [
"Depto de Gen\u00e9tica e Biologia Molecular, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Nogueira",
"givenName": "Eduardo M.",
"id": "sg:person.014204126304.31",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014204126304.31"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Laborat\u00f3rio de Gen\u00e9tica e Bioqu\u00edmica, Embrapa Agrobiologia, Serop\u00e9dica, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Guedes",
"givenName": "Helma V.",
"id": "sg:person.01276362455.00",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276362455.00"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Federal University of Rio de Janeiro",
"id": "https://www.grid.ac/institutes/grid.8536.8",
"name": [
"Laborat\u00f3rio de Biologia Molecular, Instituto de Bioqu\u00edmica M\u00e9dica, Universidade Federal do Rio de Janeiro, CEP 21941-590, Rio de Janeiro, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Oliveira",
"givenName": "Pedro L.",
"id": "sg:person.011411141517.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011411141517.49"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Federal University of Rio de Janeiro",
"id": "https://www.grid.ac/institutes/grid.8536.8",
"name": [
"Instituto Microbiologia Prof. Paulo de G\u00f3es., Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "C\u00e2mara",
"givenName": "Fernando",
"type": "Person"
},
{
"affiliation": {
"name": [
"Laborat\u00f3rio de Gen\u00e9tica e Bioqu\u00edmica, Embrapa Agrobiologia, Serop\u00e9dica, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Baldani",
"givenName": "Jose I.",
"id": "sg:person.014360631331.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014360631331.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Federal University of Rio de Janeiro",
"id": "https://www.grid.ac/institutes/grid.8536.8",
"name": [
"Laborat\u00f3rio de Biologia Molecular, Instituto de Bioqu\u00edmica M\u00e9dica, Universidade Federal do Rio de Janeiro, CEP 21941-590, Rio de Janeiro, RJ, Brazil"
],
"type": "Organization"
},
"familyName": "Martins",
"givenName": "Orlando B.",
"id": "sg:person.01067106474.72",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067106474.72"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1128/jb.184.20.5805-5809.2002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000337368"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbabio.2006.06.013",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004511196"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.mi.34.100180.001151",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010002521"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02370096",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010309406",
"https://doi.org/10.1007/bf02370096"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02370096",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010309406",
"https://doi.org/10.1007/bf02370096"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.67.10.4694-4700.2001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011085544"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1471-2164-10-450",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013449585",
"https://doi.org/10.1186/1471-2164-10-450"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0038-0717(96)00218-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017045589"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002030050672",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019121308",
"https://doi.org/10.1007/s002030050672"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1046/j.1365-2958.2000.02178.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022715000"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1083/jcb.200605036",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024115298"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0076-6879(84)05016-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024172047"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(76)90527-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025529346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/meth.2001.1262",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027621591"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/meth.2001.1262",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027621591"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi9020378",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027741263"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi9020378",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027741263"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1205-1499",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030341885",
"https://doi.org/10.1038/nbt1205-1499"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1205-1499",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030341885",
"https://doi.org/10.1038/nbt1205-1499"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2958.2005.04457.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032908612"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jb.187.1.168-174.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035851042"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jxb/erl173",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036875525"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj1340707",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037130801"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj1340707",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037130801"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.freeradbiomed.2009.05.004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041068338"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4615-9689-9_9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048268692",
"https://doi.org/10.1007/978-1-4615-9689-9_9"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/mp/ssn090",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050362056"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00168a001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055163526"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1094/mpmi.2001.14.3.358",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060080371"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1094/mpmi.2003.16.3.217",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060080660"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/00221287-54-3-463",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060373706"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/ijs.0.63149-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060386375"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1104/pp.105.4.1139",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060840417"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074548908",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079410336",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082587278",
"type": "CreativeWork"
}
],
"datePublished": "2010-10",
"datePublishedReg": "2010-10-01",
"description": "Gluconacetobacter diazotrophicus, an endophyte isolated from sugarcane, is a strict aerobe that fixates N(2). This process is catalyzed by nitrogenase and requires copious amounts of ATP. Nitrogenase activity is extremely sensitive to inhibition by oxygen and reactive oxygen species (ROS). However, the elevated oxidative metabolic rates required to sustain biological nitrogen fixation (BNF) may favor an increased production of ROS. Here, we explored this paradox and observed that ROS levels are, in fact, decreased in nitrogen-fixing cells due to the up-regulation of transcript levels of six ROS-detoxifying genes. A cluster analyses based on common expression patterns revealed the existence of a stable cluster with 99.8% similarity made up of the genes encoding the \u03b1-subunit of nitrogenase Mo-Fe protein (nifD), superoxide dismutase (sodA) and catalase type E (katE). Finally, nitrogenase activity was inhibited in a dose-dependent manner by paraquat, a redox cycler that increases cellular ROS levels. Our data revealed that ROS can strongly inhibit nitrogenase activity, and G. diazotrophicus alters its redox metabolism during BNF by increasing antioxidant transcript levels resulting in a lower ROS generation. We suggest that careful controlled ROS production during this critical phase is an adaptive mechanism to allow nitrogen fixation.",
"genre": "research_article",
"id": "sg:pub.10.1007/s00203-010-0609-1",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1018945",
"issn": [
"0302-8933",
"1432-072X"
],
"name": "Archives of Microbiology",
"type": "Periodical"
},
{
"issueNumber": "10",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "192"
}
],
"name": "Antioxidant pathways are up-regulated during biological nitrogen fixation to prevent ROS-induced nitrogenase inhibition in Gluconacetobacter diazotrophicus",
"pagination": "835-841",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"f42d18388b77d4b5361efc3fb34132b6eabab1cde49460e691b73fcdffa9e889"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"20697694"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0410427"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00203-010-0609-1"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1051258849"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00203-010-0609-1",
"https://app.dimensions.ai/details/publication/pub.1051258849"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T10:48",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000350_0000000350/records_77544_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/s00203-010-0609-1"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00203-010-0609-1'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00203-010-0609-1'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00203-010-0609-1'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00203-010-0609-1'
This table displays all metadata directly associated to this object as RDF triples.
256 TRIPLES
21 PREDICATES
69 URIs
30 LITERALS
18 BLANK NODES