Ontology type: schema:ScholarlyArticle
2016-08-16
AUTHORSHanna Jangö, Søren Gräs, Lise Christensen, Gunnar Lose
ABSTRACTIntroduction and hypothesisAlternative approaches to reinforce the native tissue in patients with pelvic organ prolapse (POP) are needed to improve surgical outcome. Our aims were to develop a weakened abdominal wall in a rat model to mimic the weakened vaginal wall in women with POP and then evaluate the regenerative potential of a quickly biodegradable synthetic scaffold, methoxypolyethylene glycol polylactic-co-glycolic acid (MPEG-PLGA), seeded with autologous muscle fiber fragments (MFFs) using this model.MethodsIn an initial pilot study with 15 animals, significant weakening of the abdominal wall and a feasible technique was established by creating a partial defect with removal of one abdominal muscle layer. Subsequently, 18 rats were evenly divided into three groups: (1) unrepaired partial defect; (2) partial defect repaired with MPEG-PLGA; (3) partial defect repaired with MPEG-PLGA and MFFs labeled with PKH26-fluorescence dye. After 8 weeks, we performed histopathological and immunohistochemical testing, fluorescence analysis, and uniaxial biomechanical testing.ResultsBoth macroscopically and microscopically, the MPEG-PLGA scaffold was fully degraded, with no signs of an inflammatory or foreign-body response. PKH26-positive cells were found in all animals from the group with added MFFs. Analysis of variance (ANOVA) showed a significant difference between groups with respect to load at failure (p = 0.028), and post hoc testing revealed that the group with MPEG-PLGA and MFFs showed a significantly higher strength than the group with MPEG-PLGA alone (p = 0.034).ConclusionTissue-engineering with MFFs seeded on a scaffold of biodegradable MPEG-PLGA might be an interesting adjunct to future POP repair. More... »
PAGES223-229
http://scigraph.springernature.com/pub.10.1007/s00192-016-3091-8
DOIhttp://dx.doi.org/10.1007/s00192-016-3091-8
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1038350199
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/27530522
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Clinical Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Abdominal Wall",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Analysis of Variance",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biocompatible Materials",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Drug Carriers",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Models, Animal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Muscle Fibers, Skeletal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Organic Chemicals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pelvic Organ Prolapse",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pilot Projects",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polyethylene Glycols",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Random Allocation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tissue Engineering",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tissue Scaffolds",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Gynecology and Obstetrics, Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK - 2730, Herlev, Denmark",
"id": "http://www.grid.ac/institutes/grid.411900.d",
"name": [
"Department of Gynecology and Obstetrics, Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK - 2730, Herlev, Denmark"
],
"type": "Organization"
},
"familyName": "Jang\u00f6",
"givenName": "Hanna",
"id": "sg:person.0716330756.09",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716330756.09"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gynecology and Obstetrics, Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK - 2730, Herlev, Denmark",
"id": "http://www.grid.ac/institutes/grid.411900.d",
"name": [
"Department of Gynecology and Obstetrics, Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK - 2730, Herlev, Denmark"
],
"type": "Organization"
},
"familyName": "Gr\u00e4s",
"givenName": "S\u00f8ren",
"id": "sg:person.0653774573.94",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653774573.94"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Copenhagen University Hospital Herlev, Herlev, Denmark",
"id": "http://www.grid.ac/institutes/grid.411900.d",
"name": [
"Department of Pathology, Copenhagen University Hospital Herlev, Herlev, Denmark"
],
"type": "Organization"
},
"familyName": "Christensen",
"givenName": "Lise",
"id": "sg:person.07424553602.99",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07424553602.99"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gynecology and Obstetrics, Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK - 2730, Herlev, Denmark",
"id": "http://www.grid.ac/institutes/grid.411900.d",
"name": [
"Department of Gynecology and Obstetrics, Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK - 2730, Herlev, Denmark"
],
"type": "Organization"
},
"familyName": "Lose",
"givenName": "Gunnar",
"id": "sg:person.01266412310.90",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266412310.90"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s11934-014-0425-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008159079",
"https://doi.org/10.1007/s11934-014-0425-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00441-014-2006-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001727952",
"https://doi.org/10.1007/s00441-014-2006-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00192-015-2782-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036909291",
"https://doi.org/10.1007/s00192-015-2782-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35102181",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038337010",
"https://doi.org/10.1038/35102181"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00192-008-0781-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000040193",
"https://doi.org/10.1007/s00192-008-0781-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00192-012-1927-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021105743",
"https://doi.org/10.1007/s00192-012-1927-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00192-012-2002-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005020365",
"https://doi.org/10.1007/s00192-012-2002-x"
],
"type": "CreativeWork"
}
],
"datePublished": "2016-08-16",
"datePublishedReg": "2016-08-16",
"description": "Introduction and hypothesisAlternative approaches to reinforce the native tissue in patients with pelvic organ prolapse (POP) are needed to improve surgical outcome. Our aims were to develop a weakened abdominal wall in a rat model to mimic the weakened vaginal wall in women with POP and then evaluate the regenerative potential of a quickly biodegradable synthetic scaffold, methoxypolyethylene glycol polylactic-co-glycolic acid (MPEG-PLGA), seeded with autologous muscle fiber fragments (MFFs) using this model.MethodsIn an initial pilot study with 15 animals, significant weakening of the abdominal wall and a feasible technique was established by creating a partial defect with removal of one abdominal muscle layer. Subsequently, 18 rats were evenly divided into three groups: (1) unrepaired partial defect; (2) partial defect repaired with MPEG-PLGA; (3) partial defect repaired with MPEG-PLGA and MFFs labeled with PKH26-fluorescence dye. After 8\u00a0weeks, we performed histopathological and immunohistochemical testing, fluorescence analysis, and uniaxial biomechanical testing.ResultsBoth macroscopically and microscopically, the MPEG-PLGA scaffold was fully degraded, with no signs of an inflammatory or foreign-body response. PKH26-positive cells were found in all animals from the group with added MFFs. Analysis of variance (ANOVA) showed a significant difference between groups with respect to load at failure (p\u2009=\u20090.028), and post hoc testing revealed that the group with MPEG-PLGA and MFFs showed a significantly higher strength than the group with MPEG-PLGA alone (p\u2009=\u20090.034).ConclusionTissue-engineering with MFFs seeded on a scaffold of biodegradable MPEG-PLGA might be an interesting adjunct to future POP repair.",
"genre": "article",
"id": "sg:pub.10.1007/s00192-016-3091-8",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1045594",
"issn": [
"0937-3462",
"1433-3023"
],
"name": "International Urogynecology Journal",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "28"
}
],
"keywords": [
"pelvic organ prolapse",
"abdominal wall",
"muscle fiber fragments",
"PKH26-positive cells",
"partial defect",
"mPEG-PLGA",
"abdominal muscle layers",
"MPEG-PLGA scaffold",
"weak abdominal wall",
"surgical outcomes",
"POP repair",
"immunohistochemical testing",
"organ prolapse",
"rat model",
"vaginal wall",
"muscle layer",
"initial pilot study",
"pilot study",
"biomechanical testing",
"analysis of variance",
"regenerative potential",
"significant differences",
"feasible technique",
"foreign body response",
"interesting adjunct",
"rats",
"group",
"animals",
"prolapse",
"patients",
"MethodsIn",
"testing",
"adjunct",
"weeks",
"women",
"defects",
"outcomes",
"ResultsBoth",
"fiber fragments",
"tissue",
"signs",
"repair",
"glycolic acid",
"cells",
"native tissue",
"failure",
"wall",
"aim",
"response",
"differences",
"methoxypolyethylene glycol",
"study",
"post",
"synthetic scaffolds",
"analysis",
"fragments",
"acid",
"scaffolds",
"removal",
"potential",
"model",
"variance",
"introduction",
"technique",
"glycol",
"respect",
"strength",
"approach",
"fluorescence analysis",
"dye",
"significant weakening",
"weakening",
"biodegradable synthetic scaffolds",
"layer",
"high strength"
],
"name": "Tissue-engineering with muscle fiber fragments improves the strength of a weak abdominal wall in rats",
"pagination": "223-229",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1038350199"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00192-016-3091-8"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"27530522"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00192-016-3091-8",
"https://app.dimensions.ai/details/publication/pub.1038350199"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:31",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_691.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00192-016-3091-8"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00192-016-3091-8'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00192-016-3091-8'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00192-016-3091-8'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00192-016-3091-8'
This table displays all metadata directly associated to this object as RDF triples.
256 TRIPLES
22 PREDICATES
125 URIs
110 LITERALS
24 BLANK NODES