AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-02

AUTHORS

A. Srivastava, V. Gupta, D. Pental, A. K. Pradhan

ABSTRACT

AFLP markers were employed to assess the genetic diversity amongst 21 established natural and nine synthetic varietes and lines of Brassica juncea originating from Asia, Australia, Canada, Eastern Europe and Russia. Six of the synthetics used for diversity studies have been developed recently. Twenty one EcoRI/MseI-based AFLP primer pairs generated a total of 1251 scorable fragments among the 30 genotypes studied, of which 778 bands were polymorphic with an average of 37 polymorphic bands per primer pair. On the basis of the similarity coefficients (F value), cluster analysis was performed using the UPGMA method. The 30 B. juncea lines could be grouped into three distinct clusters. All the Indian, Chinese and previously developed synthetics formed one cluster (cluster A), the recently developed synthetics formed a separate cluster (cluster B) and the lines from Australia, Canada, Eastern Europe and Russia formed the third cluster (cluster C). A majority of the lines were uniquely identified by one or more primer pairs due to the presence or absence of variety specific band(s). Four primer pairs were found to be most informative, since these uniquely identified all the genotypes assayed. These four primer pairs, could therefore be used as fingerprinting primers for varietal identification. More... »

PAGES

193-199

Journal

TITLE

Theoretical and Applied Genetics

ISSUE

2-3

VOLUME

102

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s001220051635

DOI

http://dx.doi.org/10.1007/s001220051635

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026795799


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