Expression of active barley seed ribosome-inactivating protein in transgenic wheat View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-03

AUTHORS

S. Bieri, I. Potrykus, J. Fütterer

ABSTRACT

Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed ß-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis. More... »

PAGES

755-763

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s001220051349

DOI

http://dx.doi.org/10.1007/s001220051349

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033122868


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