Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-08

AUTHORS

Y. Nishizawa, Z. Nishio, K. Nakazono, M. Soma, E. Nakajima, M. Ugaki, T. Hibi

ABSTRACT

Rice blast is the most devastating plant disease in Japan. Our goal is to create new rice varieties which show enhanced resistance against blast, regardless of the race of blast. By an Agrobacterium-mediated transformation method, we reintroduced a rice class-I chitinase gene, Cht-2 or Cht-3, under the control of the enhanced CaMV 35S promoter and a hygromycin phosphotransferase gene, as a selection marker into the Japonica rice varieties Nipponbare and Koshihikari, which have retained the best popularity over a long period in Japan. In regenerated plants (R0), the Cht-2 product was found to accumulate intracellularly whereas the Cht-3 product was found to be targeted extracellularly. The transgenic rice plants which constitutively expressed either chitinase gene showed significantly higher resistance against the rice blast pathogen Magnaporthe grisea races 007.0 and 333. Both high-level expression of the chitinase and blast-resistance were stably inherited by the next generation in several lines. More... »

PAGES

383-390

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s001220051248

DOI

http://dx.doi.org/10.1007/s001220051248

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019011249

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22665169


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Agricultural and Veterinary Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Crop and Pasture Production", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP", 
          "id": "http://www.grid.ac/institutes/grid.410590.9", 
          "name": [
            "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nishizawa", 
        "givenName": "Y.", 
        "id": "sg:person.01230736347.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230736347.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP", 
          "id": "http://www.grid.ac/institutes/grid.410590.9", 
          "name": [
            "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nishio", 
        "givenName": "Z.", 
        "id": "sg:person.013264725151.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013264725151.22"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Nakazono", 
        "givenName": "K.", 
        "id": "sg:person.01304000672.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304000672.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi, 113-8657, Tokyo, Japan, JP", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi, 113-8657, Tokyo, Japan, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Soma", 
        "givenName": "M.", 
        "id": "sg:person.01352114072.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352114072.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP", 
          "id": "http://www.grid.ac/institutes/grid.410590.9", 
          "name": [
            "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakajima", 
        "givenName": "E.", 
        "id": "sg:person.0626507772.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626507772.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP", 
          "id": "http://www.grid.ac/institutes/grid.410590.9", 
          "name": [
            "National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305\u20138602, Japan, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ugaki", 
        "givenName": "M.", 
        "id": "sg:person.01134310775.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134310775.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi, 113-8657, Tokyo, Japan, JP", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi, 113-8657, Tokyo, Japan, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hibi", 
        "givenName": "T.", 
        "id": "sg:person.015162370531.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015162370531.59"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1999-08", 
    "datePublishedReg": "1999-08-01", 
    "description": "Abstract\u2002Rice blast is the most devastating plant disease in Japan. Our goal is to create new rice varieties which show enhanced resistance against blast, regardless of the race of blast. By an Agrobacterium-mediated transformation method, we reintroduced a rice class-I chitinase gene, Cht-2 or Cht-3, under the control of the enhanced CaMV 35S promoter and a hygromycin phosphotransferase gene, as a selection marker into the Japonica rice varieties Nipponbare and Koshihikari, which have retained the best popularity over a long period in Japan. In regenerated plants (R0), the Cht-2 product was found to accumulate intracellularly whereas the Cht-3 product was found to be targeted extracellularly. The transgenic rice plants which constitutively expressed either chitinase gene showed significantly higher resistance against the rice blast pathogen Magnaporthe grisea races 007.0 and 333. Both high-level expression of the chitinase and blast-resistance were stably inherited by the next generation in several lines.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s001220051248", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1135804", 
        "issn": [
          "0040-5752", 
          "1432-2242"
        ], 
        "name": "Theoretical and Applied Genetics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3-4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "99"
      }
    ], 
    "keywords": [
      "chitinase gene", 
      "rice blast pathogen Magnaporthe grisea", 
      "Agrobacterium-mediated transformation method", 
      "japonica rice variety Nipponbare", 
      "transgenic rice plants", 
      "blast pathogen Magnaporthe grisea", 
      "rice variety Nipponbare", 
      "CaMV 35S promoter", 
      "devastating plant diseases", 
      "pathogen Magnaporthe grisea", 
      "hygromycin phosphotransferase gene", 
      "transgenic japonica rice", 
      "high-level expression", 
      "new rice varieties", 
      "regenerated plants", 
      "variety Nipponbare", 
      "Magnaporthe grisea", 
      "rice chitinase", 
      "plant diseases", 
      "phosphotransferase gene", 
      "selection marker", 
      "rice plants", 
      "rice blast", 
      "constitutive expression", 
      "enhanced resistance", 
      "japonica rice", 
      "rice varieties", 
      "genes", 
      "chitinase", 
      "plants", 
      "expression", 
      "Nipponbare", 
      "grisea", 
      "promoter", 
      "Koshihikari", 
      "rice", 
      "good popularity", 
      "high resistance", 
      "blasts", 
      "resistance", 
      "next generation", 
      "transformation method", 
      "markers", 
      "lines", 
      "long period", 
      "variety", 
      "products", 
      "Japan", 
      "disease", 
      "generation", 
      "control", 
      "period", 
      "race", 
      "goal", 
      "method", 
      "popularity"
    ], 
    "name": "Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase", 
    "pagination": "383-390", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1019011249"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s001220051248"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22665169"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s001220051248", 
      "https://app.dimensions.ai/details/publication/pub.1019011249"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_336.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s001220051248"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s001220051248'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s001220051248'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s001220051248'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s001220051248'


 

This table displays all metadata directly associated to this object as RDF triples.

173 TRIPLES      20 PREDICATES      85 URIs      74 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s001220051248 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 anzsrc-for:0607
4 anzsrc-for:07
5 anzsrc-for:0703
6 schema:author Ncee0a15a90b5499bb4fbcd851f0905de
7 schema:datePublished 1999-08
8 schema:datePublishedReg 1999-08-01
9 schema:description Abstract Rice blast is the most devastating plant disease in Japan. Our goal is to create new rice varieties which show enhanced resistance against blast, regardless of the race of blast. By an Agrobacterium-mediated transformation method, we reintroduced a rice class-I chitinase gene, Cht-2 or Cht-3, under the control of the enhanced CaMV 35S promoter and a hygromycin phosphotransferase gene, as a selection marker into the Japonica rice varieties Nipponbare and Koshihikari, which have retained the best popularity over a long period in Japan. In regenerated plants (R0), the Cht-2 product was found to accumulate intracellularly whereas the Cht-3 product was found to be targeted extracellularly. The transgenic rice plants which constitutively expressed either chitinase gene showed significantly higher resistance against the rice blast pathogen Magnaporthe grisea races 007.0 and 333. Both high-level expression of the chitinase and blast-resistance were stably inherited by the next generation in several lines.
10 schema:genre article
11 schema:isAccessibleForFree false
12 schema:isPartOf N15cb95e7e8b74b2d8ff54a8f0480b01c
13 Na511b69d0e244a86b13f97d755ddd298
14 sg:journal.1135804
15 schema:keywords Agrobacterium-mediated transformation method
16 CaMV 35S promoter
17 Japan
18 Koshihikari
19 Magnaporthe grisea
20 Nipponbare
21 blast pathogen Magnaporthe grisea
22 blasts
23 chitinase
24 chitinase gene
25 constitutive expression
26 control
27 devastating plant diseases
28 disease
29 enhanced resistance
30 expression
31 generation
32 genes
33 goal
34 good popularity
35 grisea
36 high resistance
37 high-level expression
38 hygromycin phosphotransferase gene
39 japonica rice
40 japonica rice variety Nipponbare
41 lines
42 long period
43 markers
44 method
45 new rice varieties
46 next generation
47 pathogen Magnaporthe grisea
48 period
49 phosphotransferase gene
50 plant diseases
51 plants
52 popularity
53 products
54 promoter
55 race
56 regenerated plants
57 resistance
58 rice
59 rice blast
60 rice blast pathogen Magnaporthe grisea
61 rice chitinase
62 rice plants
63 rice varieties
64 rice variety Nipponbare
65 selection marker
66 transformation method
67 transgenic japonica rice
68 transgenic rice plants
69 variety
70 variety Nipponbare
71 schema:name Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase
72 schema:pagination 383-390
73 schema:productId N4bfb01b8b9314f4bbd6f65455320be88
74 N7b9ffdc2b1594d619aebfa11613c51e3
75 Nf093a2cc8eef4d9088534d62e76d5666
76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019011249
77 https://doi.org/10.1007/s001220051248
78 schema:sdDatePublished 2022-08-04T16:53
79 schema:sdLicense https://scigraph.springernature.com/explorer/license/
80 schema:sdPublisher N5e948eb6dba24f68b05279c1b642b4e7
81 schema:url https://doi.org/10.1007/s001220051248
82 sgo:license sg:explorer/license/
83 sgo:sdDataset articles
84 rdf:type schema:ScholarlyArticle
85 N15cb95e7e8b74b2d8ff54a8f0480b01c schema:volumeNumber 99
86 rdf:type schema:PublicationVolume
87 N296f1f941d8e4bb6bb79286c123b4221 rdf:first sg:person.01352114072.36
88 rdf:rest N554dfe0850fc450791545a45666fd362
89 N4bfb01b8b9314f4bbd6f65455320be88 schema:name doi
90 schema:value 10.1007/s001220051248
91 rdf:type schema:PropertyValue
92 N554dfe0850fc450791545a45666fd362 rdf:first sg:person.0626507772.38
93 rdf:rest Nfd0cf083c2ae4b9e9680e05df011d82f
94 N5e948eb6dba24f68b05279c1b642b4e7 schema:name Springer Nature - SN SciGraph project
95 rdf:type schema:Organization
96 N72df7a11912b4104966c0c449603997b rdf:first sg:person.01304000672.88
97 rdf:rest N296f1f941d8e4bb6bb79286c123b4221
98 N7b9ffdc2b1594d619aebfa11613c51e3 schema:name dimensions_id
99 schema:value pub.1019011249
100 rdf:type schema:PropertyValue
101 Na511b69d0e244a86b13f97d755ddd298 schema:issueNumber 3-4
102 rdf:type schema:PublicationIssue
103 Nb528d918709f499995428b96ac4e00d5 rdf:first sg:person.015162370531.59
104 rdf:rest rdf:nil
105 Ncee0a15a90b5499bb4fbcd851f0905de rdf:first sg:person.01230736347.54
106 rdf:rest Nf14d49de79d4427eb5c534fe75297168
107 Nf093a2cc8eef4d9088534d62e76d5666 schema:name pubmed_id
108 schema:value 22665169
109 rdf:type schema:PropertyValue
110 Nf14d49de79d4427eb5c534fe75297168 rdf:first sg:person.013264725151.22
111 rdf:rest N72df7a11912b4104966c0c449603997b
112 Nfd0cf083c2ae4b9e9680e05df011d82f rdf:first sg:person.01134310775.20
113 rdf:rest Nb528d918709f499995428b96ac4e00d5
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
118 schema:name Genetics
119 rdf:type schema:DefinedTerm
120 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
121 schema:name Plant Biology
122 rdf:type schema:DefinedTerm
123 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
124 schema:name Agricultural and Veterinary Sciences
125 rdf:type schema:DefinedTerm
126 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
127 schema:name Crop and Pasture Production
128 rdf:type schema:DefinedTerm
129 sg:journal.1135804 schema:issn 0040-5752
130 1432-2242
131 schema:name Theoretical and Applied Genetics
132 schema:publisher Springer Nature
133 rdf:type schema:Periodical
134 sg:person.01134310775.20 schema:affiliation grid-institutes:grid.410590.9
135 schema:familyName Ugaki
136 schema:givenName M.
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134310775.20
138 rdf:type schema:Person
139 sg:person.01230736347.54 schema:affiliation grid-institutes:grid.410590.9
140 schema:familyName Nishizawa
141 schema:givenName Y.
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230736347.54
143 rdf:type schema:Person
144 sg:person.01304000672.88 schema:familyName Nakazono
145 schema:givenName K.
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304000672.88
147 rdf:type schema:Person
148 sg:person.013264725151.22 schema:affiliation grid-institutes:grid.410590.9
149 schema:familyName Nishio
150 schema:givenName Z.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013264725151.22
152 rdf:type schema:Person
153 sg:person.01352114072.36 schema:affiliation grid-institutes:grid.26999.3d
154 schema:familyName Soma
155 schema:givenName M.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352114072.36
157 rdf:type schema:Person
158 sg:person.015162370531.59 schema:affiliation grid-institutes:grid.26999.3d
159 schema:familyName Hibi
160 schema:givenName T.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015162370531.59
162 rdf:type schema:Person
163 sg:person.0626507772.38 schema:affiliation grid-institutes:grid.410590.9
164 schema:familyName Nakajima
165 schema:givenName E.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626507772.38
167 rdf:type schema:Person
168 grid-institutes:grid.26999.3d schema:alternateName Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi, 113-8657, Tokyo, Japan, JP
169 schema:name Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi, 113-8657, Tokyo, Japan, JP
170 rdf:type schema:Organization
171 grid-institutes:grid.410590.9 schema:alternateName National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305–8602, Japan, JP
172 schema:name National Institute of Agrobiological Resources, 2-1-2,Kannondai, Tsukuba, 305–8602, Japan, JP
173 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...