Mutation in the putative ketoacyl-ACP reductase CaKR1 induces loss of pungency in Capsicum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-01

AUTHORS

Sota Koeda, Kosuke Sato, Hiroki Saito, Atsushi J. Nagano, Masaki Yasugi, Hiroshi Kudoh, Yoshiyuki Tanaka

ABSTRACT

KEY MESSAGE: A putative ketoacyl-ACP reductase (CaKR1) that was not previously known to be associated with pungency of Capsicum was identified from map-based cloning and functional characterization. The pungency of chili pepper fruits is due to the presence of capsaicinoids, which are synthesized through the convergence of the phenylpropanoid and branched-chain fatty acid pathways. The extensive, global use of pungent and non-pungent peppers underlines the importance of understanding the genetic mechanism underlying capsaicinoid biosynthesis for breeding pepper cultivars. Although Capsicum is one of the earliest domesticated plant genera, the only reported genetic causes of its loss of pungency are mutations in acyltransferase (Pun1) and putative aminotransferase (pAMT). In this study, a single recessive gene responsible for the non-pungency of pepper No.3341 (C. chinense) was identified on chromosome 10 using an F2 population derived from a cross between Habanero and No.3341. Five candidate genes were identified in the target region, within a distance of 220 kb. A candidate gene, a putative ketoacyl-ACP reductase (CaKR1), of No.3341 had an insertion of a 4.5-kb transposable element (TE) sequence in the first intron, resulting in the production of a truncated transcript missing the region coding the catalytic domain. Virus-induced gene silencing of CaKR1 in pungent peppers resulted in the decreased accumulation of capsaicinoids, a phenotype consistent with No.3341. Moreover, GC-MS analysis of 8-methyl-6-nonenoic acid, which is predicted to be synthesized during the elongation cycle of branched-chain fatty acid biosynthesis, revealed that its deficiency in No.3341. Genetic, genomic, transcriptional, silencing, and biochemical precursor analyses performed in combination provide a solid ground for the conclusion that CaKR1 is involved in capsaicinoid biosynthesis and that its disruption results in a loss of pungency. More... »

PAGES

1-16

References to SciGraph publications

  • 2011-05. Molecular biology of capsaicinoid biosynthesis in chili pepper (Capsicum spp.) in PLANT CELL REPORTS
  • 2006-03. Two different transposable elements inserted in flavonoid 3′,5′-hydroxylase gene contribute to pink flower coloration in Gentiana scabra in MOLECULAR GENETICS AND GENOMICS
  • 2015-06. Multiple loss-of-function putative aminotransferase alleles contribute to low pungency and capsinoid biosynthesis in Capsicum chinense in MOLECULAR BREEDING
  • 2016-12. Genome-wide Diversity and Association Mapping for Capsaicinoids and Fruit Weight in Capsicum annuum L in SCIENTIFIC REPORTS
  • 1993-10. cDNA cloning and characterisation of novel ripening-related mRNAs with altered patterns of accumulation in the ripening inhibitor (rin) tomato ripening mutant in PLANT MOLECULAR BIOLOGY
  • 2004-06. The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia in THEORETICAL AND APPLIED GENETICS
  • 2008-02. Virus-induced silencing of Comt, pAmt and Kas genes results in a reduction of capsaicinoid accumulation in chili pepper fruits in PLANTA
  • 1997-07. Genetic resources and breeding of Capsicum spp. in EUPHYTICA
  • 1996-01. Gibberellins regulate the abundance of RNAs with sequence similarity to proteinase inhibitors, dioxygenases and dehydrogenases in PLANTA
  • 2016-08. Fine mapping and identification of candidate genes for the sy-2 locus in a temperature-sensitive chili pepper (Capsicum chinense) in THEORETICAL AND APPLIED GENETICS
  • 2015-12. A low-pungency S3212 genotype of Capsicum frutescens caused by a mutation in the putative aminotransferase (p-AMT) gene in MOLECULAR GENETICS AND GENOMICS
  • 2010-05. Contrasting modes for loss of pungency between cultivated and wild species of Capsicum in HEREDITY
  • 2014-03. Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species in NATURE GENETICS
  • 2017-12. Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit in PLANT METHODS
  • 1990-11. Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot-like structures expressing introduced genes in PLANT CELL REPORTS
  • 2015-12. High-throughput linkage mapping of Australian white cypress pine (Callitris glaucophylla) and map transferability to related species in TREE GENETICS & GENOMES
  • 2008-12. Medium- and short-chain dehydrogenase/reductase gene and protein families in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2015-12. Evidence of capsaicin synthase activity of the Pun1-encoded protein and its role as a determinant of capsaicinoid accumulation in pepper in BMC PLANT BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00122-018-3195-2

    DOI

    http://dx.doi.org/10.1007/s00122-018-3195-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1107303350

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30267113


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Kyoto University", 
              "id": "https://www.grid.ac/institutes/grid.258799.8", 
              "name": [
                "Faculty of Agriculture, Kindai University, 631-8505, Nara, Nara, Japan", 
                "Experimental Farm, Graduate School of Agriculture, Kyoto University, 619-0218, Kizugawa, Kyoto, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Koeda", 
            "givenName": "Sota", 
            "id": "sg:person.01061500737.46", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061500737.46"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyoto University", 
              "id": "https://www.grid.ac/institutes/grid.258799.8", 
              "name": [
                "Experimental Farm, Graduate School of Agriculture, Kyoto University, 619-0218, Kizugawa, Kyoto, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sato", 
            "givenName": "Kosuke", 
            "id": "sg:person.012735030757.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012735030757.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Japan International Research Center for Agricultural Sciences", 
              "id": "https://www.grid.ac/institutes/grid.452611.5", 
              "name": [
                "Experimental Farm, Graduate School of Agriculture, Kyoto University, 619-0218, Kizugawa, Kyoto, Japan", 
                "Tropical Agriculture Research Front, Japan International Research Center for Agricultural Sciences, 907-0002, Ishigaki, Okinawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Saito", 
            "givenName": "Hiroki", 
            "id": "sg:person.01033750405.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033750405.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Ryukoku University", 
              "id": "https://www.grid.ac/institutes/grid.440926.d", 
              "name": [
                "Faculty of Agriculture, Ryukoku University, 520-2914, Otsu, Shiga, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nagano", 
            "givenName": "Atsushi J.", 
            "id": "sg:person.01237051463.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237051463.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Utsunomiya University", 
              "id": "https://www.grid.ac/institutes/grid.267687.a", 
              "name": [
                "Faculty of Engineering, Utsunomiya University, 321-8585, Utsunomiya, Tochigi, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yasugi", 
            "givenName": "Masaki", 
            "id": "sg:person.01231405606.95", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231405606.95"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyoto University", 
              "id": "https://www.grid.ac/institutes/grid.258799.8", 
              "name": [
                "Center for Ecological Research, Kyoto University, 520-2113, Otsu, Shiga, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kudoh", 
            "givenName": "Hiroshi", 
            "id": "sg:person.0732133164.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732133164.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Okayama University", 
              "id": "https://www.grid.ac/institutes/grid.261356.5", 
              "name": [
                "Graduate School of Environmental and Life Science, Okayama University, 700-8530, Okayama, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tanaka", 
            "givenName": "Yoshiyuki", 
            "id": "sg:person.010350437031.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010350437031.20"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.2503/hortj.mi-049", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000289680"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00197589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001280368", 
              "https://doi.org/10.1007/bf00197589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00197589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001280368", 
              "https://doi.org/10.1007/bf00197589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biotechadv.2009.08.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002078336"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pmic.200600326", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002961474"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232399", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003112708", 
              "https://doi.org/10.1007/bf00232399"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232399", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003112708", 
              "https://doi.org/10.1007/bf00232399"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0969-2126(00)00115-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003644882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1155/2014/194812", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005157885"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(90)90721-p", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005930066"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.2877", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006131701", 
              "https://doi.org/10.1038/ng.2877"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jxb/erl243", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007191017"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btm404", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007683223"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-9452(99)00118-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008537583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep38081", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008826502", 
              "https://doi.org/10.1038/srep38081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/j39680000442", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009461425"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-004-1625-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009958150", 
              "https://doi.org/10.1007/s00122-004-1625-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13007-016-0151-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011330872", 
              "https://doi.org/10.1186/s13007-016-0151-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13007-016-0151-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011330872", 
              "https://doi.org/10.1186/s13007-016-0151-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2503/hortj.mi-148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012010747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/molbev/msw054", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012564990"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0048156", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012792194"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/0735-260291044269", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012928900"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-015-0339-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012972195", 
              "https://doi.org/10.1007/s11032-015-0339-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1136914", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013910209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2503/jjshs.58.353", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017345587"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11295-015-0944-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018697154", 
              "https://doi.org/10.1007/s11295-015-0944-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bfgp/elu002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018823072"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.109.136549", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019743994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0009-2797(02)00223-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021269979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0009-2797(02)00223-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021269979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jxb/erg176", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021514513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2503/jjshs1.ch-105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023371300"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2009.03921.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024501746"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2009.03921.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024501746"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1002913228101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024955196", 
              "https://doi.org/10.1023/a:1002913228101"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2001.00993.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026494245"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.2009.131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026772509", 
              "https://doi.org/10.1038/hdy.2009.131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.2009.131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026772509", 
              "https://doi.org/10.1038/hdy.2009.131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1400975111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028464440"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.121.2.479", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035077070"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-016-2723-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036592815", 
              "https://doi.org/10.1007/s00122-016-2723-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00018-008-8588-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036976559", 
              "https://doi.org/10.1007/s00018-008-8588-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00018-008-8588-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036976559", 
              "https://doi.org/10.1007/s00018-008-8588-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/g3.111.000240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037130935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/g3.111.000240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037130935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molp.2014.11.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042295188"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btu170", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042720804"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00438-015-1071-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043473314", 
              "https://doi.org/10.1007/s00438-015-1071-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2005.02410.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044311547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2005.02410.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044311547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/10408398509527412", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044645410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.biochem.74.082803.133524", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045469555"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(93)90522-r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046174557"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00425-007-0651-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046560765", 
              "https://doi.org/10.1007/s00425-007-0651-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1742-4658.2007.05642.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049214410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-010-0968-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050232782", 
              "https://doi.org/10.1007/s00299-010-0968-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-010-0968-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050232782", 
              "https://doi.org/10.1007/s00299-010-0968-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00021431", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050305397", 
              "https://doi.org/10.1007/bf00021431"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00021431", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050305397", 
              "https://doi.org/10.1007/bf00021431"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00438-005-0083-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050761600", 
              "https://doi.org/10.1007/s00438-005-0083-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00438-005-0083-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050761600", 
              "https://doi.org/10.1007/s00438-005-0083-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12870-015-0476-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051968036", 
              "https://doi.org/10.1186/s12870-015-0476-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12870-015-0476-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051968036", 
              "https://doi.org/10.1186/s12870-015-0476-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja01026a049", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055762303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jf052085z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055904352"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jf052085z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055904352"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jf1019642", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055908412"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jf1019642", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055908412"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jf903282r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055927455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jf903282r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055927455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/aob/mcw079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059395331"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4236/ajps.2015.68127", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072383423"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074814993", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076848249", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1986.tb04517.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079947985"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083370305", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.17.00506", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085367132"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2503/hortj.okd-115", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092411324"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/pbi.12894", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100830326"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-01", 
        "datePublishedReg": "2019-01-01", 
        "description": "KEY MESSAGE: A putative ketoacyl-ACP reductase (CaKR1) that was not previously known to be associated with pungency of Capsicum was identified from map-based cloning and functional characterization. The pungency of chili pepper fruits is due to the presence of capsaicinoids, which are synthesized through the convergence of the phenylpropanoid and branched-chain fatty acid pathways. The extensive, global use of pungent and non-pungent peppers underlines the importance of understanding the genetic mechanism underlying capsaicinoid biosynthesis for breeding pepper cultivars. Although Capsicum is one of the earliest domesticated plant genera, the only reported genetic causes of its loss of pungency are mutations in acyltransferase (Pun1) and putative aminotransferase (pAMT). In this study, a single recessive gene responsible for the non-pungency of pepper No.3341 (C. chinense) was identified on chromosome 10 using an F2 population derived from a cross between Habanero and No.3341. Five candidate genes were identified in the target region, within a distance of 220\u00a0kb. A candidate gene, a putative ketoacyl-ACP reductase (CaKR1), of No.3341 had an insertion of a 4.5-kb transposable element (TE) sequence in the first intron, resulting in the production of a truncated transcript missing the region coding the catalytic domain. Virus-induced gene silencing of CaKR1 in pungent peppers resulted in the decreased accumulation of capsaicinoids, a phenotype consistent with No.3341. Moreover, GC-MS analysis of 8-methyl-6-nonenoic acid, which is predicted to be synthesized during the elongation cycle of branched-chain fatty acid biosynthesis, revealed that its deficiency in No.3341. Genetic, genomic, transcriptional, silencing, and biochemical precursor analyses performed in combination provide a solid ground for the conclusion that CaKR1 is involved in capsaicinoid biosynthesis and that its disruption results in a loss of pungency.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00122-018-3195-2", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.6131701", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5909486", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1135804", 
            "issn": [
              "0040-5752", 
              "1432-2242"
            ], 
            "name": "Theoretical and Applied Genetics", 
            "type": "Periodical"
          }
        ], 
        "name": "Mutation in the putative ketoacyl-ACP reductase CaKR1 induces loss of pungency in Capsicum", 
        "pagination": "1-16", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "e1fd161c73473bc1f9279a48c87fb45661d4bb46e62c7d5964d5589dbe8e2a0d"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30267113"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0145600"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00122-018-3195-2"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1107303350"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00122-018-3195-2", 
          "https://app.dimensions.ai/details/publication/pub.1107303350"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:42", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000541.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs00122-018-3195-2"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00122-018-3195-2'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00122-018-3195-2'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00122-018-3195-2'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00122-018-3195-2'


     

    This table displays all metadata directly associated to this object as RDF triples.

    331 TRIPLES      21 PREDICATES      91 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00122-018-3195-2 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author Nb59fe1c8cf0d46c59ef494227910dd5c
    4 schema:citation sg:pub.10.1007/bf00021431
    5 sg:pub.10.1007/bf00197589
    6 sg:pub.10.1007/bf00232399
    7 sg:pub.10.1007/s00018-008-8588-y
    8 sg:pub.10.1007/s00122-004-1625-9
    9 sg:pub.10.1007/s00122-016-2723-1
    10 sg:pub.10.1007/s00299-010-0968-8
    11 sg:pub.10.1007/s00425-007-0651-7
    12 sg:pub.10.1007/s00438-005-0083-7
    13 sg:pub.10.1007/s00438-015-1071-1
    14 sg:pub.10.1007/s11032-015-0339-9
    15 sg:pub.10.1007/s11295-015-0944-0
    16 sg:pub.10.1023/a:1002913228101
    17 sg:pub.10.1038/hdy.2009.131
    18 sg:pub.10.1038/ng.2877
    19 sg:pub.10.1038/srep38081
    20 sg:pub.10.1186/s12870-015-0476-7
    21 sg:pub.10.1186/s13007-016-0151-5
    22 https://app.dimensions.ai/details/publication/pub.1074814993
    23 https://app.dimensions.ai/details/publication/pub.1076848249
    24 https://app.dimensions.ai/details/publication/pub.1083370305
    25 https://doi.org/10.1002/j.1460-2075.1986.tb04517.x
    26 https://doi.org/10.1002/pmic.200600326
    27 https://doi.org/10.1016/0092-8674(90)90721-p
    28 https://doi.org/10.1016/0092-8674(93)90522-r
    29 https://doi.org/10.1016/j.biotechadv.2009.08.005
    30 https://doi.org/10.1016/j.molp.2014.11.024
    31 https://doi.org/10.1016/s0009-2797(02)00223-5
    32 https://doi.org/10.1016/s0168-9452(99)00118-1
    33 https://doi.org/10.1016/s0969-2126(00)00115-5
    34 https://doi.org/10.1021/ja01026a049
    35 https://doi.org/10.1021/jf052085z
    36 https://doi.org/10.1021/jf1019642
    37 https://doi.org/10.1021/jf903282r
    38 https://doi.org/10.1039/j39680000442
    39 https://doi.org/10.1046/j.1365-313x.2001.00993.x
    40 https://doi.org/10.1073/pnas.1400975111
    41 https://doi.org/10.1080/0735-260291044269
    42 https://doi.org/10.1080/10408398509527412
    43 https://doi.org/10.1093/aob/mcw079
    44 https://doi.org/10.1093/bfgp/elu002
    45 https://doi.org/10.1093/bioinformatics/btm404
    46 https://doi.org/10.1093/bioinformatics/btu170
    47 https://doi.org/10.1093/jxb/erg176
    48 https://doi.org/10.1093/jxb/erl243
    49 https://doi.org/10.1093/molbev/msw054
    50 https://doi.org/10.1104/pp.109.136549
    51 https://doi.org/10.1104/pp.121.2.479
    52 https://doi.org/10.1104/pp.17.00506
    53 https://doi.org/10.1111/j.1365-313x.2005.02410.x
    54 https://doi.org/10.1111/j.1365-313x.2009.03921.x
    55 https://doi.org/10.1111/j.1742-4658.2007.05642.x
    56 https://doi.org/10.1111/pbi.12894
    57 https://doi.org/10.1126/science.1136914
    58 https://doi.org/10.1146/annurev.biochem.74.082803.133524
    59 https://doi.org/10.1155/2014/194812
    60 https://doi.org/10.1371/journal.pone.0048156
    61 https://doi.org/10.1534/g3.111.000240
    62 https://doi.org/10.2503/hortj.mi-049
    63 https://doi.org/10.2503/hortj.mi-148
    64 https://doi.org/10.2503/hortj.okd-115
    65 https://doi.org/10.2503/jjshs.58.353
    66 https://doi.org/10.2503/jjshs1.ch-105
    67 https://doi.org/10.4236/ajps.2015.68127
    68 schema:datePublished 2019-01
    69 schema:datePublishedReg 2019-01-01
    70 schema:description KEY MESSAGE: A putative ketoacyl-ACP reductase (CaKR1) that was not previously known to be associated with pungency of Capsicum was identified from map-based cloning and functional characterization. The pungency of chili pepper fruits is due to the presence of capsaicinoids, which are synthesized through the convergence of the phenylpropanoid and branched-chain fatty acid pathways. The extensive, global use of pungent and non-pungent peppers underlines the importance of understanding the genetic mechanism underlying capsaicinoid biosynthesis for breeding pepper cultivars. Although Capsicum is one of the earliest domesticated plant genera, the only reported genetic causes of its loss of pungency are mutations in acyltransferase (Pun1) and putative aminotransferase (pAMT). In this study, a single recessive gene responsible for the non-pungency of pepper No.3341 (C. chinense) was identified on chromosome 10 using an F2 population derived from a cross between Habanero and No.3341. Five candidate genes were identified in the target region, within a distance of 220 kb. A candidate gene, a putative ketoacyl-ACP reductase (CaKR1), of No.3341 had an insertion of a 4.5-kb transposable element (TE) sequence in the first intron, resulting in the production of a truncated transcript missing the region coding the catalytic domain. Virus-induced gene silencing of CaKR1 in pungent peppers resulted in the decreased accumulation of capsaicinoids, a phenotype consistent with No.3341. Moreover, GC-MS analysis of 8-methyl-6-nonenoic acid, which is predicted to be synthesized during the elongation cycle of branched-chain fatty acid biosynthesis, revealed that its deficiency in No.3341. Genetic, genomic, transcriptional, silencing, and biochemical precursor analyses performed in combination provide a solid ground for the conclusion that CaKR1 is involved in capsaicinoid biosynthesis and that its disruption results in a loss of pungency.
    71 schema:genre research_article
    72 schema:inLanguage en
    73 schema:isAccessibleForFree false
    74 schema:isPartOf sg:journal.1135804
    75 schema:name Mutation in the putative ketoacyl-ACP reductase CaKR1 induces loss of pungency in Capsicum
    76 schema:pagination 1-16
    77 schema:productId N28019a9b165e4674b3aa4198e4187656
    78 N4f6117c77af54f6aac3529969f179e4b
    79 N65fd7bb136e4486bbbd4cb09805ce820
    80 N84c8f5e329b34ce792bfe2a9121d6c8b
    81 Neadc7e8aafaf44a990e3bf41eae909b1
    82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107303350
    83 https://doi.org/10.1007/s00122-018-3195-2
    84 schema:sdDatePublished 2019-04-10T21:42
    85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    86 schema:sdPublisher Nf57bdcc352d441d5a8295b6863167f00
    87 schema:url https://link.springer.com/10.1007%2Fs00122-018-3195-2
    88 sgo:license sg:explorer/license/
    89 sgo:sdDataset articles
    90 rdf:type schema:ScholarlyArticle
    91 N176ad271742543149b4ce69f8abf3c82 rdf:first sg:person.010350437031.20
    92 rdf:rest rdf:nil
    93 N1a326ad4e0424a8b860e869b42b52f01 rdf:first sg:person.01033750405.37
    94 rdf:rest N6cd886a2a2d040ccb53325c20bc4c601
    95 N28019a9b165e4674b3aa4198e4187656 schema:name dimensions_id
    96 schema:value pub.1107303350
    97 rdf:type schema:PropertyValue
    98 N394c9854acc14473826fbf4927c81709 rdf:first sg:person.01231405606.95
    99 rdf:rest N8359c83f5744460390d97233b5c3b085
    100 N4f6117c77af54f6aac3529969f179e4b schema:name readcube_id
    101 schema:value e1fd161c73473bc1f9279a48c87fb45661d4bb46e62c7d5964d5589dbe8e2a0d
    102 rdf:type schema:PropertyValue
    103 N65fd7bb136e4486bbbd4cb09805ce820 schema:name doi
    104 schema:value 10.1007/s00122-018-3195-2
    105 rdf:type schema:PropertyValue
    106 N6cd886a2a2d040ccb53325c20bc4c601 rdf:first sg:person.01237051463.54
    107 rdf:rest N394c9854acc14473826fbf4927c81709
    108 N8359c83f5744460390d97233b5c3b085 rdf:first sg:person.0732133164.54
    109 rdf:rest N176ad271742543149b4ce69f8abf3c82
    110 N84c8f5e329b34ce792bfe2a9121d6c8b schema:name nlm_unique_id
    111 schema:value 0145600
    112 rdf:type schema:PropertyValue
    113 N9087c4dd37a04e718d423217f619c910 rdf:first sg:person.012735030757.16
    114 rdf:rest N1a326ad4e0424a8b860e869b42b52f01
    115 Nb59fe1c8cf0d46c59ef494227910dd5c rdf:first sg:person.01061500737.46
    116 rdf:rest N9087c4dd37a04e718d423217f619c910
    117 Neadc7e8aafaf44a990e3bf41eae909b1 schema:name pubmed_id
    118 schema:value 30267113
    119 rdf:type schema:PropertyValue
    120 Nf57bdcc352d441d5a8295b6863167f00 schema:name Springer Nature - SN SciGraph project
    121 rdf:type schema:Organization
    122 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    123 schema:name Biological Sciences
    124 rdf:type schema:DefinedTerm
    125 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    126 schema:name Genetics
    127 rdf:type schema:DefinedTerm
    128 sg:grant.5909486 http://pending.schema.org/fundedItem sg:pub.10.1007/s00122-018-3195-2
    129 rdf:type schema:MonetaryGrant
    130 sg:grant.6131701 http://pending.schema.org/fundedItem sg:pub.10.1007/s00122-018-3195-2
    131 rdf:type schema:MonetaryGrant
    132 sg:journal.1135804 schema:issn 0040-5752
    133 1432-2242
    134 schema:name Theoretical and Applied Genetics
    135 rdf:type schema:Periodical
    136 sg:person.01033750405.37 schema:affiliation https://www.grid.ac/institutes/grid.452611.5
    137 schema:familyName Saito
    138 schema:givenName Hiroki
    139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033750405.37
    140 rdf:type schema:Person
    141 sg:person.010350437031.20 schema:affiliation https://www.grid.ac/institutes/grid.261356.5
    142 schema:familyName Tanaka
    143 schema:givenName Yoshiyuki
    144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010350437031.20
    145 rdf:type schema:Person
    146 sg:person.01061500737.46 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
    147 schema:familyName Koeda
    148 schema:givenName Sota
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061500737.46
    150 rdf:type schema:Person
    151 sg:person.01231405606.95 schema:affiliation https://www.grid.ac/institutes/grid.267687.a
    152 schema:familyName Yasugi
    153 schema:givenName Masaki
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231405606.95
    155 rdf:type schema:Person
    156 sg:person.01237051463.54 schema:affiliation https://www.grid.ac/institutes/grid.440926.d
    157 schema:familyName Nagano
    158 schema:givenName Atsushi J.
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237051463.54
    160 rdf:type schema:Person
    161 sg:person.012735030757.16 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
    162 schema:familyName Sato
    163 schema:givenName Kosuke
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012735030757.16
    165 rdf:type schema:Person
    166 sg:person.0732133164.54 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
    167 schema:familyName Kudoh
    168 schema:givenName Hiroshi
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732133164.54
    170 rdf:type schema:Person
    171 sg:pub.10.1007/bf00021431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050305397
    172 https://doi.org/10.1007/bf00021431
    173 rdf:type schema:CreativeWork
    174 sg:pub.10.1007/bf00197589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001280368
    175 https://doi.org/10.1007/bf00197589
    176 rdf:type schema:CreativeWork
    177 sg:pub.10.1007/bf00232399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003112708
    178 https://doi.org/10.1007/bf00232399
    179 rdf:type schema:CreativeWork
    180 sg:pub.10.1007/s00018-008-8588-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1036976559
    181 https://doi.org/10.1007/s00018-008-8588-y
    182 rdf:type schema:CreativeWork
    183 sg:pub.10.1007/s00122-004-1625-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009958150
    184 https://doi.org/10.1007/s00122-004-1625-9
    185 rdf:type schema:CreativeWork
    186 sg:pub.10.1007/s00122-016-2723-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036592815
    187 https://doi.org/10.1007/s00122-016-2723-1
    188 rdf:type schema:CreativeWork
    189 sg:pub.10.1007/s00299-010-0968-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050232782
    190 https://doi.org/10.1007/s00299-010-0968-8
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.1007/s00425-007-0651-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046560765
    193 https://doi.org/10.1007/s00425-007-0651-7
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1007/s00438-005-0083-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050761600
    196 https://doi.org/10.1007/s00438-005-0083-7
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1007/s00438-015-1071-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043473314
    199 https://doi.org/10.1007/s00438-015-1071-1
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1007/s11032-015-0339-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012972195
    202 https://doi.org/10.1007/s11032-015-0339-9
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1007/s11295-015-0944-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018697154
    205 https://doi.org/10.1007/s11295-015-0944-0
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1023/a:1002913228101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024955196
    208 https://doi.org/10.1023/a:1002913228101
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1038/hdy.2009.131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026772509
    211 https://doi.org/10.1038/hdy.2009.131
    212 rdf:type schema:CreativeWork
    213 sg:pub.10.1038/ng.2877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006131701
    214 https://doi.org/10.1038/ng.2877
    215 rdf:type schema:CreativeWork
    216 sg:pub.10.1038/srep38081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008826502
    217 https://doi.org/10.1038/srep38081
    218 rdf:type schema:CreativeWork
    219 sg:pub.10.1186/s12870-015-0476-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051968036
    220 https://doi.org/10.1186/s12870-015-0476-7
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1186/s13007-016-0151-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011330872
    223 https://doi.org/10.1186/s13007-016-0151-5
    224 rdf:type schema:CreativeWork
    225 https://app.dimensions.ai/details/publication/pub.1074814993 schema:CreativeWork
    226 https://app.dimensions.ai/details/publication/pub.1076848249 schema:CreativeWork
    227 https://app.dimensions.ai/details/publication/pub.1083370305 schema:CreativeWork
    228 https://doi.org/10.1002/j.1460-2075.1986.tb04517.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079947985
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1002/pmic.200600326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002961474
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1016/0092-8674(90)90721-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1005930066
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1016/0092-8674(93)90522-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1046174557
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/j.biotechadv.2009.08.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002078336
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1016/j.molp.2014.11.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042295188
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1016/s0009-2797(02)00223-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021269979
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1016/s0168-9452(99)00118-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008537583
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1016/s0969-2126(00)00115-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003644882
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1021/ja01026a049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055762303
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1021/jf052085z schema:sameAs https://app.dimensions.ai/details/publication/pub.1055904352
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1021/jf1019642 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055908412
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1021/jf903282r schema:sameAs https://app.dimensions.ai/details/publication/pub.1055927455
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1039/j39680000442 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009461425
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1046/j.1365-313x.2001.00993.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026494245
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1073/pnas.1400975111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028464440
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1080/0735-260291044269 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012928900
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1080/10408398509527412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044645410
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1093/aob/mcw079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059395331
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1093/bfgp/elu002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018823072
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1093/bioinformatics/btm404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007683223
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1093/bioinformatics/btu170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042720804
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1093/jxb/erg176 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021514513
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1093/jxb/erl243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007191017
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1093/molbev/msw054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012564990
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1104/pp.109.136549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019743994
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1104/pp.121.2.479 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035077070
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1104/pp.17.00506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085367132
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1111/j.1365-313x.2005.02410.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044311547
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1111/j.1365-313x.2009.03921.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024501746
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1111/j.1742-4658.2007.05642.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049214410
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1111/pbi.12894 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100830326
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1126/science.1136914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013910209
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1146/annurev.biochem.74.082803.133524 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045469555
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1155/2014/194812 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005157885
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1371/journal.pone.0048156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012792194
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1534/g3.111.000240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037130935
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.2503/hortj.mi-049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000289680
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.2503/hortj.mi-148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012010747
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.2503/hortj.okd-115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092411324
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.2503/jjshs.58.353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017345587
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.2503/jjshs1.ch-105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023371300
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.4236/ajps.2015.68127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072383423
    313 rdf:type schema:CreativeWork
    314 https://www.grid.ac/institutes/grid.258799.8 schema:alternateName Kyoto University
    315 schema:name Center for Ecological Research, Kyoto University, 520-2113, Otsu, Shiga, Japan
    316 Experimental Farm, Graduate School of Agriculture, Kyoto University, 619-0218, Kizugawa, Kyoto, Japan
    317 Faculty of Agriculture, Kindai University, 631-8505, Nara, Nara, Japan
    318 rdf:type schema:Organization
    319 https://www.grid.ac/institutes/grid.261356.5 schema:alternateName Okayama University
    320 schema:name Graduate School of Environmental and Life Science, Okayama University, 700-8530, Okayama, Japan
    321 rdf:type schema:Organization
    322 https://www.grid.ac/institutes/grid.267687.a schema:alternateName Utsunomiya University
    323 schema:name Faculty of Engineering, Utsunomiya University, 321-8585, Utsunomiya, Tochigi, Japan
    324 rdf:type schema:Organization
    325 https://www.grid.ac/institutes/grid.440926.d schema:alternateName Ryukoku University
    326 schema:name Faculty of Agriculture, Ryukoku University, 520-2914, Otsu, Shiga, Japan
    327 rdf:type schema:Organization
    328 https://www.grid.ac/institutes/grid.452611.5 schema:alternateName Japan International Research Center for Agricultural Sciences
    329 schema:name Experimental Farm, Graduate School of Agriculture, Kyoto University, 619-0218, Kizugawa, Kyoto, Japan
    330 Tropical Agriculture Research Front, Japan International Research Center for Agricultural Sciences, 907-0002, Ishigaki, Okinawa, Japan
    331 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...