Recessive male sterility in cabbage (Brassica oleracea var. capitata) caused by loss of function of BoCYP704B1 due to the insertion ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-04-12

AUTHORS

Jia-lei Ji, Li-mei Yang, Zhi-yuan Fang, Mu Zhuang, Yang-yong Zhang, Hong-hao Lv, Yu-mei Liu, Zhan-sheng Li

ABSTRACT

Key messageThe LTR-retrotransposon insertion in BoCYP704B1 is proved to be the primary cause of the male sterility in cabbage. Effective allele-specific markers were developed for marker-assisted selection of male sterile gene.Abstract83121A is a spontaneous male sterile mutant identified from cabbage. Genetic analysis indicated that male sterility is controlled by a single recessive gene. Pollen wall formation in the 83121A mutant was severely defective, with a lack of sporopollenin or exine. To understand the mechanisms of male sterility in 83121A, transcription analysis using RNA-Seq was carried out in the buds of the male sterile line 83121A and the male fertile line 83121B, which are near-isogenic lines differing only in the fertility trait. Via expression analysis of differentially expressed genes involved in pollen exine development before the bicellular pollen stage, BoCYP704B1 was identified as a candidate gene, which was approximately downregulated 30-fold in 83121A. BoCYP704B1 is a member of the evolutionarily conserved CYP704B family, which is essential for sporopollenin formation. The BoCYP704B1 transcript is specifically detected in the developing anthers of wild-type cabbage. Further sequence analysis revealed that a 5424-bp long terminal repeat-retrotransposon (LTR-RT) was inserted into the first exon of BoCYP704B1 in 83121A, which is not found in wild-type plants. The insertion of LTR-RT not only reduced the expression of BoCYP704B1 but also altered structure of protein encoded by BoCYP704B1. Moreover, linkage analysis showed that the homozygotic mutational BoCYP704B1 always cosegregated with male sterility. These data suggest that the LTR-RT insertion in BoCYP704B1 hinders sporopollenin formation in 83121A leading to male sterility. The allele-specific markers developed in this study were effective for marker-assisted selection of the male sterile gene. More... »

PAGES

1441-1451

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00122-017-2899-z

DOI

http://dx.doi.org/10.1007/s00122-017-2899-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084818344

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28405714


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brassica", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytochrome P-450 Enzyme System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Recessive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Markers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Infertility", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pollen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Retroelements", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ji", 
        "givenName": "Jia-lei", 
        "id": "sg:person.016451164500.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016451164500.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Li-mei", 
        "id": "sg:person.07616173155.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07616173155.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fang", 
        "givenName": "Zhi-yuan", 
        "id": "sg:person.01126421156.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126421156.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhuang", 
        "givenName": "Mu", 
        "id": "sg:person.0650135735.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650135735.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Yang-yong", 
        "id": "sg:person.0620136207.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0620136207.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lv", 
        "givenName": "Hong-hao", 
        "id": "sg:person.01254616141.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254616141.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Yu-mei", 
        "id": "sg:person.01002500007.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002500007.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.410727.7", 
          "name": [
            "Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Zhan-sheng", 
        "id": "sg:person.011541570761.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011541570761.97"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nrg3374", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041941568", 
          "https://doi.org/10.1038/nrg3374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004380000436", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030088645", 
          "https://doi.org/10.1007/s004380000436"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11103-013-0085-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044588730", 
          "https://doi.org/10.1007/s11103-013-0085-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1226", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045381177", 
          "https://doi.org/10.1038/nmeth.1226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms4930", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005748112", 
          "https://doi.org/10.1038/ncomms4930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep13059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003744405", 
          "https://doi.org/10.1038/srep13059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2009-10-3-r25", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049583368", 
          "https://doi.org/10.1186/gb-2009-10-3-r25"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11103-015-0287-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024843102", 
          "https://doi.org/10.1007/s11103-015-0287-0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-04-12", 
    "datePublishedReg": "2017-04-12", 
    "description": "Key messageThe LTR-retrotransposon insertion in BoCYP704B1 is proved to be the primary cause of the male sterility in cabbage. Effective allele-specific markers were developed for marker-assisted selection of male sterile gene.Abstract83121A is a spontaneous male sterile mutant identified from cabbage. Genetic analysis indicated that male sterility is controlled by a single recessive gene. Pollen wall formation in the 83121A mutant was severely defective, with a lack of sporopollenin or exine. To understand the mechanisms of male sterility in 83121A, transcription analysis using RNA-Seq was carried out in the buds of the male sterile line 83121A and the male fertile line 83121B, which are near-isogenic lines differing only in the fertility trait. Via expression analysis of differentially expressed genes involved in pollen exine development before the bicellular pollen stage, BoCYP704B1 was identified as a candidate gene, which was approximately downregulated 30-fold in 83121A. BoCYP704B1 is a member of the evolutionarily conserved CYP704B family, which is essential for sporopollenin formation. The BoCYP704B1 transcript is specifically detected in the developing anthers of wild-type cabbage. Further sequence analysis revealed that a 5424-bp long terminal repeat-retrotransposon (LTR-RT) was inserted into the first exon of BoCYP704B1 in 83121A, which is not found in wild-type plants. The insertion of LTR-RT not only reduced the expression of BoCYP704B1 but also altered structure of protein encoded by BoCYP704B1. Moreover, linkage analysis showed that the homozygotic mutational BoCYP704B1 always cosegregated with male sterility. These data suggest that the LTR-RT insertion in BoCYP704B1 hinders sporopollenin formation in 83121A leading to male sterility. The allele-specific markers developed in this study were effective for marker-assisted selection of the male sterile gene.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00122-017-2899-z", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1135804", 
        "issn": [
          "0040-5752", 
          "1432-2242"
        ], 
        "name": "Theoretical and Applied Genetics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "130"
      }
    ], 
    "keywords": [
      "marker-assisted selection", 
      "allele-specific markers", 
      "male sterile gene", 
      "male sterility", 
      "sterile genes", 
      "sporopollenin formation", 
      "LTR-retrotransposon insertion", 
      "LTR-RT insertion", 
      "wild-type plants", 
      "spontaneous male sterile mutant", 
      "recessive male sterility", 
      "male sterile mutant", 
      "LTR-retrotransposons", 
      "pollen exine development", 
      "pollen wall formation", 
      "bicellular pollen stage", 
      "single recessive gene", 
      "LTR-RT", 
      "RNA-seq", 
      "Further sequence analysis", 
      "sterile mutants", 
      "structure of proteins", 
      "transcription analysis", 
      "isogenic lines", 
      "loss of function", 
      "wall formation", 
      "pollen stage", 
      "expression analysis", 
      "genetic analysis", 
      "recessive gene", 
      "first exon", 
      "candidate genes", 
      "sequence analysis", 
      "genes", 
      "fertility traits", 
      "linkage analysis", 
      "sterility", 
      "exine development", 
      "mutants", 
      "cabbage", 
      "anthers", 
      "insertion", 
      "exons", 
      "transcripts", 
      "plants", 
      "traits", 
      "protein", 
      "buds", 
      "markers", 
      "sporopollenin", 
      "selection", 
      "expression", 
      "formation", 
      "exine", 
      "family", 
      "primary cause", 
      "members", 
      "analysis", 
      "mechanism", 
      "lines", 
      "function", 
      "stage", 
      "development", 
      "loss", 
      "structure", 
      "lack", 
      "study", 
      "data", 
      "cause", 
      "Key messageThe LTR-retrotransposon insertion", 
      "messageThe LTR-retrotransposon insertion", 
      "BoCYP704B1", 
      "Effective allele-specific markers", 
      "Abstract83121A", 
      "lack of sporopollenin", 
      "male sterile line 83121A", 
      "sterile line 83121A", 
      "line 83121A", 
      "male fertile line 83121B", 
      "fertile line 83121B", 
      "line 83121B", 
      "CYP704B family", 
      "BoCYP704B1 transcript", 
      "wild-type cabbage", 
      "expression of BoCYP704B1", 
      "homozygotic mutational BoCYP704B1", 
      "mutational BoCYP704B1"
    ], 
    "name": "Recessive male sterility in cabbage (Brassica oleracea var. capitata) caused by loss of function of BoCYP704B1 due to the insertion of a LTR-retrotransposon", 
    "pagination": "1441-1451", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084818344"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00122-017-2899-z"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28405714"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00122-017-2899-z", 
      "https://app.dimensions.ai/details/publication/pub.1084818344"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_736.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00122-017-2899-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00122-017-2899-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00122-017-2899-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00122-017-2899-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00122-017-2899-z'


 

This table displays all metadata directly associated to this object as RDF triples.

270 TRIPLES      22 PREDICATES      131 URIs      115 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00122-017-2899-z schema:about N036ec9ed99b94e03a2225ea4738bf7a0
2 N152da1ee930e4656a2ab95c0fd1af1bf
3 N18f2d67d5a32483bbbe9a3dc7d1f62f1
4 N19448e85f69d4784b1d1da74dbdd7108
5 N3567762c6b1d4dc2932da1c54ed0ad7d
6 N41c5db5de116408b88cb2409bcc74922
7 N6bf851518aae411aaf37cf60760ce324
8 Nba0398f61cec4809bc96574b08d2fd5f
9 Nbd8f26dfa1db4e979d0da58519597d08
10 Ne573e6800ca94a218e9debf167c5be28
11 anzsrc-for:06
12 anzsrc-for:0604
13 schema:author N6c13d6cf143d44268adf28cc54b7abd7
14 schema:citation sg:pub.10.1007/s004380000436
15 sg:pub.10.1007/s11103-013-0085-5
16 sg:pub.10.1007/s11103-015-0287-0
17 sg:pub.10.1038/ncomms4930
18 sg:pub.10.1038/nmeth.1226
19 sg:pub.10.1038/nrg3374
20 sg:pub.10.1038/srep13059
21 sg:pub.10.1186/gb-2009-10-3-r25
22 schema:datePublished 2017-04-12
23 schema:datePublishedReg 2017-04-12
24 schema:description Key messageThe LTR-retrotransposon insertion in BoCYP704B1 is proved to be the primary cause of the male sterility in cabbage. Effective allele-specific markers were developed for marker-assisted selection of male sterile gene.Abstract83121A is a spontaneous male sterile mutant identified from cabbage. Genetic analysis indicated that male sterility is controlled by a single recessive gene. Pollen wall formation in the 83121A mutant was severely defective, with a lack of sporopollenin or exine. To understand the mechanisms of male sterility in 83121A, transcription analysis using RNA-Seq was carried out in the buds of the male sterile line 83121A and the male fertile line 83121B, which are near-isogenic lines differing only in the fertility trait. Via expression analysis of differentially expressed genes involved in pollen exine development before the bicellular pollen stage, BoCYP704B1 was identified as a candidate gene, which was approximately downregulated 30-fold in 83121A. BoCYP704B1 is a member of the evolutionarily conserved CYP704B family, which is essential for sporopollenin formation. The BoCYP704B1 transcript is specifically detected in the developing anthers of wild-type cabbage. Further sequence analysis revealed that a 5424-bp long terminal repeat-retrotransposon (LTR-RT) was inserted into the first exon of BoCYP704B1 in 83121A, which is not found in wild-type plants. The insertion of LTR-RT not only reduced the expression of BoCYP704B1 but also altered structure of protein encoded by BoCYP704B1. Moreover, linkage analysis showed that the homozygotic mutational BoCYP704B1 always cosegregated with male sterility. These data suggest that the LTR-RT insertion in BoCYP704B1 hinders sporopollenin formation in 83121A leading to male sterility. The allele-specific markers developed in this study were effective for marker-assisted selection of the male sterile gene.
25 schema:genre article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N078e9686c4f84f318d22e22e8b9144da
29 Nab18976d13e1462aa187247451222d09
30 sg:journal.1135804
31 schema:keywords Abstract83121A
32 BoCYP704B1
33 BoCYP704B1 transcript
34 CYP704B family
35 Effective allele-specific markers
36 Further sequence analysis
37 Key messageThe LTR-retrotransposon insertion
38 LTR-RT
39 LTR-RT insertion
40 LTR-retrotransposon insertion
41 LTR-retrotransposons
42 RNA-seq
43 allele-specific markers
44 analysis
45 anthers
46 bicellular pollen stage
47 buds
48 cabbage
49 candidate genes
50 cause
51 data
52 development
53 exine
54 exine development
55 exons
56 expression
57 expression analysis
58 expression of BoCYP704B1
59 family
60 fertile line 83121B
61 fertility traits
62 first exon
63 formation
64 function
65 genes
66 genetic analysis
67 homozygotic mutational BoCYP704B1
68 insertion
69 isogenic lines
70 lack
71 lack of sporopollenin
72 line 83121A
73 line 83121B
74 lines
75 linkage analysis
76 loss
77 loss of function
78 male fertile line 83121B
79 male sterile gene
80 male sterile line 83121A
81 male sterile mutant
82 male sterility
83 marker-assisted selection
84 markers
85 mechanism
86 members
87 messageThe LTR-retrotransposon insertion
88 mutants
89 mutational BoCYP704B1
90 plants
91 pollen exine development
92 pollen stage
93 pollen wall formation
94 primary cause
95 protein
96 recessive gene
97 recessive male sterility
98 selection
99 sequence analysis
100 single recessive gene
101 spontaneous male sterile mutant
102 sporopollenin
103 sporopollenin formation
104 stage
105 sterile genes
106 sterile line 83121A
107 sterile mutants
108 sterility
109 structure
110 structure of proteins
111 study
112 traits
113 transcription analysis
114 transcripts
115 wall formation
116 wild-type cabbage
117 wild-type plants
118 schema:name Recessive male sterility in cabbage (Brassica oleracea var. capitata) caused by loss of function of BoCYP704B1 due to the insertion of a LTR-retrotransposon
119 schema:pagination 1441-1451
120 schema:productId N27581042ccc04ea3959769c551aeba9d
121 N2ab8cd65ee9f40e09f68663895e68097
122 N5b3e229bd7b347a182079ad7bbb5d37c
123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084818344
124 https://doi.org/10.1007/s00122-017-2899-z
125 schema:sdDatePublished 2021-11-01T18:30
126 schema:sdLicense https://scigraph.springernature.com/explorer/license/
127 schema:sdPublisher N467f4560e00047e6af131f0d28950dad
128 schema:url https://doi.org/10.1007/s00122-017-2899-z
129 sgo:license sg:explorer/license/
130 sgo:sdDataset articles
131 rdf:type schema:ScholarlyArticle
132 N036ec9ed99b94e03a2225ea4738bf7a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Genetic Markers
134 rdf:type schema:DefinedTerm
135 N078e9686c4f84f318d22e22e8b9144da schema:volumeNumber 130
136 rdf:type schema:PublicationVolume
137 N152da1ee930e4656a2ab95c0fd1af1bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Base Sequence
139 rdf:type schema:DefinedTerm
140 N18f2d67d5a32483bbbe9a3dc7d1f62f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Genes, Plant
142 rdf:type schema:DefinedTerm
143 N19448e85f69d4784b1d1da74dbdd7108 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Retroelements
145 rdf:type schema:DefinedTerm
146 N1c584eab8ccd49e2b3dfe24fce145078 rdf:first sg:person.011541570761.97
147 rdf:rest rdf:nil
148 N27581042ccc04ea3959769c551aeba9d schema:name doi
149 schema:value 10.1007/s00122-017-2899-z
150 rdf:type schema:PropertyValue
151 N2ab8cd65ee9f40e09f68663895e68097 schema:name pubmed_id
152 schema:value 28405714
153 rdf:type schema:PropertyValue
154 N3567762c6b1d4dc2932da1c54ed0ad7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Brassica
156 rdf:type schema:DefinedTerm
157 N40db3f74ab9a436ca99ea14a8d4e820d rdf:first sg:person.01126421156.25
158 rdf:rest N62ab424bf7b7469aa296c5191fff1117
159 N41c5db5de116408b88cb2409bcc74922 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Cytochrome P-450 Enzyme System
161 rdf:type schema:DefinedTerm
162 N467f4560e00047e6af131f0d28950dad schema:name Springer Nature - SN SciGraph project
163 rdf:type schema:Organization
164 N5b3e229bd7b347a182079ad7bbb5d37c schema:name dimensions_id
165 schema:value pub.1084818344
166 rdf:type schema:PropertyValue
167 N62ab424bf7b7469aa296c5191fff1117 rdf:first sg:person.0650135735.89
168 rdf:rest N9580a070f0264a4aab9817f576cdbee9
169 N6bf851518aae411aaf37cf60760ce324 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Plant Infertility
171 rdf:type schema:DefinedTerm
172 N6c13d6cf143d44268adf28cc54b7abd7 rdf:first sg:person.016451164500.40
173 rdf:rest N9e4b6b8b3ded436988594a09ed68193c
174 N9580a070f0264a4aab9817f576cdbee9 rdf:first sg:person.0620136207.47
175 rdf:rest Ne01679b61a17495e845d30b085fa3ffd
176 N9e4b6b8b3ded436988594a09ed68193c rdf:first sg:person.07616173155.19
177 rdf:rest N40db3f74ab9a436ca99ea14a8d4e820d
178 Nab18976d13e1462aa187247451222d09 schema:issueNumber 7
179 rdf:type schema:PublicationIssue
180 Nba0398f61cec4809bc96574b08d2fd5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Phenotype
182 rdf:type schema:DefinedTerm
183 Nbd8f26dfa1db4e979d0da58519597d08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Pollen
185 rdf:type schema:DefinedTerm
186 Nc8541dda5be34b33bbcbd1cde25ceb65 rdf:first sg:person.01002500007.27
187 rdf:rest N1c584eab8ccd49e2b3dfe24fce145078
188 Ne01679b61a17495e845d30b085fa3ffd rdf:first sg:person.01254616141.55
189 rdf:rest Nc8541dda5be34b33bbcbd1cde25ceb65
190 Ne573e6800ca94a218e9debf167c5be28 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Genes, Recessive
192 rdf:type schema:DefinedTerm
193 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
194 schema:name Biological Sciences
195 rdf:type schema:DefinedTerm
196 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
197 schema:name Genetics
198 rdf:type schema:DefinedTerm
199 sg:journal.1135804 schema:issn 0040-5752
200 1432-2242
201 schema:name Theoretical and Applied Genetics
202 schema:publisher Springer Nature
203 rdf:type schema:Periodical
204 sg:person.01002500007.27 schema:affiliation grid-institutes:grid.410727.7
205 schema:familyName Liu
206 schema:givenName Yu-mei
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002500007.27
208 rdf:type schema:Person
209 sg:person.01126421156.25 schema:affiliation grid-institutes:grid.410727.7
210 schema:familyName Fang
211 schema:givenName Zhi-yuan
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126421156.25
213 rdf:type schema:Person
214 sg:person.011541570761.97 schema:affiliation grid-institutes:grid.410727.7
215 schema:familyName Li
216 schema:givenName Zhan-sheng
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011541570761.97
218 rdf:type schema:Person
219 sg:person.01254616141.55 schema:affiliation grid-institutes:grid.410727.7
220 schema:familyName Lv
221 schema:givenName Hong-hao
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254616141.55
223 rdf:type schema:Person
224 sg:person.016451164500.40 schema:affiliation grid-institutes:grid.410727.7
225 schema:familyName Ji
226 schema:givenName Jia-lei
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016451164500.40
228 rdf:type schema:Person
229 sg:person.0620136207.47 schema:affiliation grid-institutes:grid.410727.7
230 schema:familyName Zhang
231 schema:givenName Yang-yong
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0620136207.47
233 rdf:type schema:Person
234 sg:person.0650135735.89 schema:affiliation grid-institutes:grid.410727.7
235 schema:familyName Zhuang
236 schema:givenName Mu
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650135735.89
238 rdf:type schema:Person
239 sg:person.07616173155.19 schema:affiliation grid-institutes:grid.410727.7
240 schema:familyName Yang
241 schema:givenName Li-mei
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07616173155.19
243 rdf:type schema:Person
244 sg:pub.10.1007/s004380000436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030088645
245 https://doi.org/10.1007/s004380000436
246 rdf:type schema:CreativeWork
247 sg:pub.10.1007/s11103-013-0085-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044588730
248 https://doi.org/10.1007/s11103-013-0085-5
249 rdf:type schema:CreativeWork
250 sg:pub.10.1007/s11103-015-0287-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024843102
251 https://doi.org/10.1007/s11103-015-0287-0
252 rdf:type schema:CreativeWork
253 sg:pub.10.1038/ncomms4930 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005748112
254 https://doi.org/10.1038/ncomms4930
255 rdf:type schema:CreativeWork
256 sg:pub.10.1038/nmeth.1226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045381177
257 https://doi.org/10.1038/nmeth.1226
258 rdf:type schema:CreativeWork
259 sg:pub.10.1038/nrg3374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041941568
260 https://doi.org/10.1038/nrg3374
261 rdf:type schema:CreativeWork
262 sg:pub.10.1038/srep13059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003744405
263 https://doi.org/10.1038/srep13059
264 rdf:type schema:CreativeWork
265 sg:pub.10.1186/gb-2009-10-3-r25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049583368
266 https://doi.org/10.1186/gb-2009-10-3-r25
267 rdf:type schema:CreativeWork
268 grid-institutes:grid.410727.7 schema:alternateName Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China
269 schema:name Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China
270 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...