A truncated FatB resulting from a single nucleotide insertion is responsible for reducing saturated fatty acids in maize seed oil View Full Text


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Article Info

DATE

2014-05-07

AUTHORS

Peizhong Zheng, MD Ali Babar, Seshasai Parthasarathy, Ryan Gibson, Kelly Parliament, Josh Flook, Thomas Patterson, Peter Friedemann, Siva Kumpatla, Steve Thompson

ABSTRACT

Key messageWe identified a G-nucleotide insertion in a maizeFatBresponsible for reducing saturated fatty acids through QTL mapping and map-based cloning and developed an allele-specific DNA marker for molecular breeding.AbstractVegetable oils with reduced saturated fatty acids have signficant health benefits. SRS72NE, a Dow AgroSciences proprietory maize inbred line, was found to contain signficantly reduced levels of palmitic acid and total saturated fatty acids in seed oil when compared to other common inbreds. Using F2 and F3 populations derived from a cross between SRS72NE and a normal inbred SLN74, we have demonstrated that the reduced saturated fatty acid phenotype in SRS72NE is controlled by a single QTL on chromosome 9 that explains 79.1 % of palmitic acid and 79.6 % total saturated fatty acid variations. The QTL was mapped to an interval of 105 kb that contains one single gene, a type B fatty acyl-ACP thioesterase (ZmFatB; GRMZM5G829544). ZmFatB alleles from SRS72NE and common inbreds were cloned and sequenced. SRS72NE fatb allele contains a single nucleotide (G) insertion in the 6th exon, which creates a premature stop codon 22 base pairs down stream. As a result, ZmFatB protein from SRS72NE is predicted to contain eight altered and 90 deleted amino acids at its C-terminus. Because the affected region is part of the conserved acyl-ACP thioesterase catalytic domain, the truncated ZmFatB in SRS72NE is likely non-functional. We also show that fatb RNA level in SRS72NE is reduced by 4.4-fold when compared to the normal allele SNL74. A high throughput DNA assay capable of differentiating the normal and reduced saturate fatty acid alleles has been developed and can be used for accelerated molecular breeding. More... »

PAGES

1537-1547

References to SciGraph publications

  • 2009-10-25. Major and minor QTL and epistasis contribute to fatty acid compositions and oil concentration in high-oil maize in THEORETICAL AND APPLIED GENETICS
  • 2008-02-17. A phenylalanine in DGAT is a key determinant of oil content and composition in maize in NATURE GENETICS
  • 2011-10-11. Validation of DGAT1-2 polymorphisms associated with oil content and development of functional markers for molecular breeding of high-oil maize in MOLECULAR BREEDING
  • 2012-07-31. Transposon insertion in a cinnamyl alcohol dehydrogenase gene is responsible for a brown midrib1 mutation in maize in PLANT MOLECULAR BIOLOGY
  • 2002-06-19. Chromosomal regions associated with segregation distortion in maize in THEORETICAL AND APPLIED GENETICS
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  • 2004-05. Mapping QTLs for kernel oil content in a tropical maize population in EUPHYTICA
  • 2012-06-06. Characterization of QTL for oil content in maize kernel in THEORETICAL AND APPLIED GENETICS
  • 2011-08-10. Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity in BMC MOLECULAR AND CELL BIOLOGY
  • 2007-10-13. Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize in MOLECULAR GENETICS AND GENOMICS
  • 2012-12-16. Genome-wide association study dissects the genetic architecture of oil biosynthesis in maize kernels in NATURE GENETICS
  • 2007-01-03. Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach in BMC PLANT BIOLOGY
  • 2010-01-12. QTL detection for grain oil and starch content and their associations in two connected F2:3 populations in high-oil maize in EUPHYTICA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00122-014-2317-8

    DOI

    http://dx.doi.org/10.1007/s00122-014-2317-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002374880

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24802074


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    38 schema:description Key messageWe identified a G-nucleotide insertion in a maizeFatBresponsible for reducing saturated fatty acids through QTL mapping and map-based cloning and developed an allele-specific DNA marker for molecular breeding.AbstractVegetable oils with reduced saturated fatty acids have signficant health benefits. SRS72NE, a Dow AgroSciences proprietory maize inbred line, was found to contain signficantly reduced levels of palmitic acid and total saturated fatty acids in seed oil when compared to other common inbreds. Using F2 and F3 populations derived from a cross between SRS72NE and a normal inbred SLN74, we have demonstrated that the reduced saturated fatty acid phenotype in SRS72NE is controlled by a single QTL on chromosome 9 that explains 79.1 % of palmitic acid and 79.6 % total saturated fatty acid variations. The QTL was mapped to an interval of 105 kb that contains one single gene, a type B fatty acyl-ACP thioesterase (ZmFatB; GRMZM5G829544). ZmFatB alleles from SRS72NE and common inbreds were cloned and sequenced. SRS72NE fatb allele contains a single nucleotide (G) insertion in the 6th exon, which creates a premature stop codon 22 base pairs down stream. As a result, ZmFatB protein from SRS72NE is predicted to contain eight altered and 90 deleted amino acids at its C-terminus. Because the affected region is part of the conserved acyl-ACP thioesterase catalytic domain, the truncated ZmFatB in SRS72NE is likely non-functional. We also show that fatb RNA level in SRS72NE is reduced by 4.4-fold when compared to the normal allele SNL74. A high throughput DNA assay capable of differentiating the normal and reduced saturate fatty acid alleles has been developed and can be used for accelerated molecular breeding.
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