Natural mutations in two homoeologous TT8 genes control yellow seed coat trait in allotetraploid Brassica juncea (AABB) View Full Text


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Article Info

DATE

2014-02

AUTHORS

Lakshmi K. Padmaja, Parul Agarwal, Vibha Gupta, Arundhati Mukhopadhyay, Yaspal S. Sodhi, Deepak Pental, Akshay K. Pradhan

ABSTRACT

Identification of the candidate gene responsible for the seed coat colour variation in Brassica juncea was undertaken following an earlier study where two independent loci (BjSc1 and BjSc2) were mapped to two linkage groups, LG A9 and B3 (Padmaja et al. in Theor Appl Genet 111:8-14, 2005). The genome search from BRAD data for the presence of flavonoid genes in B. rapa identified three candidate genes namely, DFR, TT1 and TT8 in the LG A9. Quantitative real-time PCR revealed absence of transcript for the late biosynthetic genes (LBGs) and showed significant reduction of transcript in the TT8 from the developing seeds of yellow-seeded line. While mapping of two DFR genes, the BjuA.DFR and BjuB.DFR did not show perfect co-segregation with the seed coat colour loci, that of the two TT8 genes, BjuA.TT8 and BjuB.TT8 showed perfect co-segregation with the seed coat colour phenotype. The BjuA.TT8 allele from the yellow-seeded line revealed the presence of an insertion of 1,279 bp in the exon 7 and did not produce any transcript as revealed by reverse transcriptase PCR. The BjuB.TT8 allele from the yellow-seeded line revealed the presence of an SNP (C→T) in the exon 7 resulting in a stop codon predicting a truncated protein lacking the C-terminal 8 amino acid residues and produced significantly low level of transcript than its wild-type counterpart. Hence, it is hypothesized that the mutations in both the TT8 genes are required for inhibiting the transcription of LBGs in the yellow-seeded mutant of B. juncea. More... »

PAGES

339-347

References to SciGraph publications

  • 2004-06. Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea (L.) Czern in THEORETICAL AND APPLIED GENETICS
  • 1994. Extraction of total cellular DNA from plants, algae and fungi in PLANT MOLECULAR BIOLOGY MANUAL
  • 2007-10. Mapping of yield influencing QTL in Brassica juncea: implications for breeding of a major oilseed crop of dryland areas in THEORETICAL AND APPLIED GENETICS
  • 2009-03. Inheritance and molecular markers for the seed coat color in Brassica juncea in FRONTIERS OF AGRICULTURE IN CHINA
  • 2004-08. Genetic analysis of agronomic and quality traits in mustard (Brassica juncea) in THEORETICAL AND APPLIED GENETICS
  • 2008-12. Comparative mapping of Brassica juncea and Arabidopsis thaliana using Intron Polymorphism (IP) markers: homoeologous relationships, diversification and evolution of the A, B and C Brassica genomes in BMC GENOMICS
  • 2003-02. A high-density linkage map in Brassica juncea (Indian mustard) using AFLP and RFLP markers in THEORETICAL AND APPLIED GENETICS
  • 2000-07. Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection in THEORETICAL AND APPLIED GENETICS
  • 2005-06. Mapping and tagging of seed coat colour and the identification of microsatellite markers for marker-assisted manipulation of the trait in Brassica juncea in THEORETICAL AND APPLIED GENETICS
  • 2008-06. Analyzing real-time PCR data by the comparative CT method in NATURE PROTOCOLS
  • 2009-03. Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00122-013-2222-6

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    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24247234


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