Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticumaestivum L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-11-07

AUTHORS

L. H. Wang, X. L. Zhao, Z. H. He, W. Ma, R. Appels, R. J. Peña, X. C. Xia

ABSTRACT

Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality. More... »

PAGES

525-539

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00122-008-0918-9

    DOI

    http://dx.doi.org/10.1007/s00122-008-0918-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014002565

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18989655


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