The reference genetic linkage map for the multinational Brassica rapa genome sequencing project View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-10

AUTHORS

Su Ryun Choi, Graham R. Teakle, Prikshit Plaha, Jeong Hee Kim, Charlotte J. Allender, Elena Beynon, Zhong Yun Piao, Pilar Soengas, Tae Ho Han, Graham J. King, Guy C. Barker, Paul Hand, Derek J. Lydiate, Jacqueline Batley, David Edwards, Dal Hoe Koo, Jae Wook Bang, Beom-Seok Park, Yong Pyo Lim

ABSTRACT

We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F(1) of a cross between two diverse Chinese cabbage (B. rapa ssp. pekinensis) inbred lines, 'Chiifu-401-42' (C) and 'Kenshin-402-43' (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1-R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species. More... »

PAGES

777-792

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00122-007-0608-z

    DOI

    http://dx.doi.org/10.1007/s00122-007-0608-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050427973

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17646962


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