BAC-derived diagnostic markers for sex determination in asparagus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-04

AUTHORS

A. Jamsari, I. Nitz, S. M. Reamon-Büttner, C. Jung

ABSTRACT

A HindIII BAC (bacterial artificial chromosome) library of asparagus ( Asparagus officinalis L.) was established from a single male plant homozygous for the male flowering gene ( MM). The library represents approximately 5.5 haploid genome equivalents with an average insert size of 82 kb. A subset of the library (2.6 haploid genome equivalents) was arranged into DNA pools. Using nine sex-linked amplified fragment length polymorphism (AFLP) and two sequence-tagged site (STS) markers, 13 different BAC clones were identified from this part of the library. The BACs were arranged into a first-generation physical map around the sex locus. Four PCR-derived markers were developed from the BAC ends, one of which could be scored in a co-dominant way. Using a mapping population of 802 plants we mapped the BAC-derived markers to the same position close to the M gene as the corresponding AFLP and STS markers. The markers are useful for further chromosome walking studies and as diagnostic markers for selecting male plants homozygous for the M gene. More... »

PAGES

1140-1146

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00122-003-1529-0

DOI

http://dx.doi.org/10.1007/s00122-003-1529-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011875370

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15067401


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