Molecular tagging of erucic acid trait in oilseed mustard (Brassica juncea) by QTL mapping and single nucleotide polymorphisms in FAE1 ... View Full Text


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Article Info

DATE

2004-02

AUTHORS

V. Gupta, A. Mukhopadhyay, N. Arumugam, Y. S. Sodhi, D. Pental, A. K. Pradhan

ABSTRACT

Molecular mapping and tagging of the erucic acid trait (C22:1) in Brassica juncea was done by a candidate gene approach. Two QTLs underlying the variation of seed erucic acid content were assigned to two linkage groups of a B. juncea map using a doubled haploid (DH) mapping population derived from high x low erucic acid F(1) hybrid. Two consensus primers corresponding to the full-length Fatty Acid Elongase 1 ( FAE1) gene, reported to be involved in the elongation of C18:1 to C22:1, were designed. PCR amplification and subsequent cloning and sequencing identified two FAE1 genes ( FAE1.1 and FAE1.2) in both high and low erucic acid mustard lines. Sequence alignment of corresponding FAE1 genes between high and low erucic acid mustard lines identified four substitution type single nucleotide polymorphisms (SNPs) in FAE1.1 and three in FAE1.2. Using the SNuPE method of SNP genotyping, these two genes were mapped to two independent loci that co-segregated with the two QTLs governing the erucic acid trait. Association of wild ( E1E2) and mutant ( e1e2) haplotypes of two FAE1 genes with erucic acid variation in two segregating populations revealed that the e1e1e2e2 genotype identified low erucic acid individuals (<2%) and E1E1E2E2 identified individuals with highest erucic acid content (>40%). The E1e1E2e2 heterozygote was found to be intermediate in phenotype. The applicability of these SNPs in marker-assisted manipulation of the erucic acid trait was verified by genotyping a set of contrasting germplasm of B. juncea belonging to two distinct gene pools (Indian and east European) and other oil-yielding Brassica species. More... »

PAGES

743-749

References to SciGraph publications

  • 1990-08. Mutants of Arabidopsis with alterations in seed lipid fatty acid composition in THEORETICAL AND APPLIED GENETICS
  • 1998-05. The two genes homologous to Arabidopsis FAE1 co-segregate with the two loci governing erucic acid content in Brassica napus in THEORETICAL AND APPLIED GENETICS
  • 1994. Extraction of total cellular DNA from plants, algae and fungi in PLANT MOLECULAR BIOLOGY MANUAL
  • 2001-12. Accessing genetic variation: genotyping single nucleotide polymorphisms in NATURE REVIEWS GENETICS
  • 2001-02. AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea in THEORETICAL AND APPLIED GENETICS
  • 2001-01. High-throughput genotyping for a polymorphism linked to soybean cyst nematode resistance gene Rhg4 by using TaqmanTM probes in MOLECULAR BREEDING
  • 1998-12. Mapping and cloning of FAD2 gene to develop allele-specific PCR for oleic acid in spring turnip rape (Brassica rapa ssp. oleifera) in MOLECULAR BREEDING
  • 2003-02. A high-density linkage map in Brassica juncea (Indian mustard) using AFLP and RFLP markers in THEORETICAL AND APPLIED GENETICS
  • 2003-02. A high-throughput SNuPE assay for genotyping SNPs in the flanking regions of Zea mays sequence tagged simple sequence repeats in MOLECULAR BREEDING
  • 1990-08. Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition in THEORETICAL AND APPLIED GENETICS
  • 2002. Molecular Marker Assisted Breeding in MOLECULAR TECHNIQUES IN CROP IMPROVEMENT
  • 2002-09. Mapping of genes affecting linolenic acid content in Brassica rapa ssp. oleifera in MOLECULAR BREEDING
  • 1996-03. Identification of RAPD markers linked to the loci controlling erucic acid level in rapeseed in MOLECULAR BREEDING
  • 1998-02. A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acid in THEORETICAL AND APPLIED GENETICS
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    URI

    http://scigraph.springernature.com/pub.10.1007/s00122-003-1481-z

    DOI

    http://dx.doi.org/10.1007/s00122-003-1481-z

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/14564400


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