Proving the Authenticity of Ancient DNA by Comparative Genomic Hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-10

AUTHORS

S. Hummel, B. Herrmann, J. Rameckers, D. Müller, K. Sperling, H. Neitzel, H. Tönnies

ABSTRACT

In PCR-supported amplification of ancient, degraded DNA, contamination with contemporary DNA can lead to false-positive results, which frequently give rise to discussions in which the mere existence of ancient DNA is doubted. Our confirmation of ancient DNA using comparative genome hybridization (CGH) eliminates these doubts. Unlike PCR methods, CGH requires no amplification of the DNA to be analyzed if adequate amounts of specimen DNA is used. Thus, false results traceable to contaminations are practically ruled out. The examples provided here prove the authenticity of ancient DNA for a 250-year-old and a 3,000-year-old sample. At the same time, the CGH of ancient DNA offers the chance to gain insight into the pattern of DNA degradation and to monitor the preservation of certain chromosomal segments. More... »

PAGES

500-503

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s001140050663

DOI

http://dx.doi.org/10.1007/s001140050663

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042756833

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10541662


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Congenital Abnormalities", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Karyotyping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Paleontology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Telomere", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Historische Anthropologie und Human\u00f6kologie, Institut f\u00fcr Zoologie und Anthropologie, Universit\u00e4t G\u00f6ttingen, B\u00fcrgerstrasse 50, D-37073 G\u00f6ttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hummel", 
        "givenName": "S.", 
        "id": "sg:person.01267211254.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267211254.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Historische Anthropologie und Human\u00f6kologie, Institut f\u00fcr Zoologie und Anthropologie, Universit\u00e4t G\u00f6ttingen, B\u00fcrgerstrasse 50, D-37073 G\u00f6ttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Herrmann", 
        "givenName": "B.", 
        "id": "sg:person.01367624564.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367624564.68"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Historische Anthropologie und Human\u00f6kologie, Institut f\u00fcr Zoologie und Anthropologie, Universit\u00e4t G\u00f6ttingen, B\u00fcrgerstrasse 50, D-37073 G\u00f6ttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rameckers", 
        "givenName": "J.", 
        "id": "sg:person.0647760022.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647760022.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Klinikum Chemnitz", 
          "id": "https://www.grid.ac/institutes/grid.459629.5", 
          "name": [
            "S\u00e4uglingsklinik, Klinikum Chemnitz, Flemmingstrasse 4, D-09009 Chemnitz, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "M\u00fcller", 
        "givenName": "D.", 
        "id": "sg:person.0635706520.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635706520.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Humboldt University of Berlin", 
          "id": "https://www.grid.ac/institutes/grid.7468.d", 
          "name": [
            "Institut f\u00fcr Humangenetik, Molekulare Zytogenetik, Charit\u00e9, Campus Virchow Klinikum, Humboldt Universit\u00e4t, Haus H, Augustenburger Platz 1, D-13353 Berlin, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sperling", 
        "givenName": "K.", 
        "id": "sg:person.0770722030.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770722030.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Humboldt University of Berlin", 
          "id": "https://www.grid.ac/institutes/grid.7468.d", 
          "name": [
            "Institut f\u00fcr Humangenetik, Molekulare Zytogenetik, Charit\u00e9, Campus Virchow Klinikum, Humboldt Universit\u00e4t, Haus H, Augustenburger Platz 1, D-13353 Berlin, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Neitzel", 
        "givenName": "H.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Humboldt University of Berlin", 
          "id": "https://www.grid.ac/institutes/grid.7468.d", 
          "name": [
            "Institut f\u00fcr Humangenetik, Molekulare Zytogenetik, Charit\u00e9, Campus Virchow Klinikum, Humboldt Universit\u00e4t, Haus H, Augustenburger Platz 1, D-13353 Berlin, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "T\u00f6nnies", 
        "givenName": "H.", 
        "id": "sg:person.01363262240.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363262240.20"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1999-10", 
    "datePublishedReg": "1999-10-01", 
    "description": "In PCR-supported amplification of ancient, degraded DNA, contamination with contemporary DNA can lead to false-positive results, which frequently give rise to discussions in which the mere existence of ancient DNA is doubted. Our confirmation of ancient DNA using comparative genome hybridization (CGH) eliminates these doubts. Unlike PCR methods, CGH requires no amplification of the DNA to be analyzed if adequate amounts of specimen DNA is used. Thus, false results traceable to contaminations are practically ruled out. The examples provided here prove the authenticity of ancient DNA for a 250-year-old and a 3,000-year-old sample. At the same time, the CGH of ancient DNA offers the chance to gain insight into the pattern of DNA degradation and to monitor the preservation of certain chromosomal segments.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s001140050663", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018189", 
        "issn": [
          "0028-1042", 
          "1432-1904"
        ], 
        "name": "The Science of Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "86"
      }
    ], 
    "name": "Proving the Authenticity of Ancient DNA by Comparative Genomic Hybridization", 
    "pagination": "500-503", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "cbab330de4a1e694dbf394b8b262ec5d3744b20c12ff248483ea0060620646e6"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10541662"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0400767"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s001140050663"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1042756833"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s001140050663", 
      "https://app.dimensions.ai/details/publication/pub.1042756833"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:41", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000507.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs001140050663"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s001140050663'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s001140050663'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s001140050663'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s001140050663'


 

This table displays all metadata directly associated to this object as RDF triples.

159 TRIPLES      20 PREDICATES      39 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s001140050663 schema:about N0e205128cf3746f491266788f6ee5007
2 N20e7ca6368da4c8db65df274c40b2d4b
3 N2fb58e7577e94483980d3c9c436fd551
4 N39f1d65826e14a7785dce15c5453240f
5 N572870ef572f4b5f92c180d00ea57ad9
6 N9ddc5cca3a8e41af967b68b57b30b902
7 Nacaa3869035b44f7a385a04366a4fd5d
8 Nbf5e1a20ee324bbfb60a0eeac9048302
9 Ne7607d4316734bc2b7c90370cb7a3c3e
10 Nf82450f8ca144b188cea06a7e07fefa7
11 anzsrc-for:06
12 anzsrc-for:0604
13 schema:author N9947ef8dd7c14870b7f3a0d8ec62286f
14 schema:datePublished 1999-10
15 schema:datePublishedReg 1999-10-01
16 schema:description In PCR-supported amplification of ancient, degraded DNA, contamination with contemporary DNA can lead to false-positive results, which frequently give rise to discussions in which the mere existence of ancient DNA is doubted. Our confirmation of ancient DNA using comparative genome hybridization (CGH) eliminates these doubts. Unlike PCR methods, CGH requires no amplification of the DNA to be analyzed if adequate amounts of specimen DNA is used. Thus, false results traceable to contaminations are practically ruled out. The examples provided here prove the authenticity of ancient DNA for a 250-year-old and a 3,000-year-old sample. At the same time, the CGH of ancient DNA offers the chance to gain insight into the pattern of DNA degradation and to monitor the preservation of certain chromosomal segments.
17 schema:genre research_article
18 schema:inLanguage en
19 schema:isAccessibleForFree false
20 schema:isPartOf N416f05596372416481811d4c2db24c4f
21 N6a9ae8fa947d40deb1757216345ab5fb
22 sg:journal.1018189
23 schema:name Proving the Authenticity of Ancient DNA by Comparative Genomic Hybridization
24 schema:pagination 500-503
25 schema:productId N14c2cfc49ec24646995e5e4a9378d214
26 N1851903bfe6c4c1ea835b075a928589c
27 N506ca5c50c254d1bbbfb95bb2b4e54c4
28 N8b6b7331d65e4fcfb6a5da22e002f714
29 Nde1555456f6d468bb349f723d598f134
30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042756833
31 https://doi.org/10.1007/s001140050663
32 schema:sdDatePublished 2019-04-10T16:41
33 schema:sdLicense https://scigraph.springernature.com/explorer/license/
34 schema:sdPublisher Neb296a9e35424996bd037cade6989c2e
35 schema:url http://link.springer.com/10.1007%2Fs001140050663
36 sgo:license sg:explorer/license/
37 sgo:sdDataset articles
38 rdf:type schema:ScholarlyArticle
39 N0e205128cf3746f491266788f6ee5007 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
40 schema:name Paleontology
41 rdf:type schema:DefinedTerm
42 N14c2cfc49ec24646995e5e4a9378d214 schema:name doi
43 schema:value 10.1007/s001140050663
44 rdf:type schema:PropertyValue
45 N1851903bfe6c4c1ea835b075a928589c schema:name pubmed_id
46 schema:value 10541662
47 rdf:type schema:PropertyValue
48 N20e7ca6368da4c8db65df274c40b2d4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
49 schema:name Karyotyping
50 rdf:type schema:DefinedTerm
51 N2fb58e7577e94483980d3c9c436fd551 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
52 schema:name Humans
53 rdf:type schema:DefinedTerm
54 N3777dddd6665444cb31a4129c86326e9 schema:name Historische Anthropologie und Humanökologie, Institut für Zoologie und Anthropologie, Universität Göttingen, Bürgerstrasse 50, D-37073 Göttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE
55 rdf:type schema:Organization
56 N39f1d65826e14a7785dce15c5453240f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
57 schema:name Telomere
58 rdf:type schema:DefinedTerm
59 N416f05596372416481811d4c2db24c4f schema:volumeNumber 86
60 rdf:type schema:PublicationVolume
61 N4356b2e30f1c41cfb7bda3fc77803581 schema:affiliation https://www.grid.ac/institutes/grid.7468.d
62 schema:familyName Neitzel
63 schema:givenName H.
64 rdf:type schema:Person
65 N44cc01ab7c2c4b18aa83d3b1ddc7b024 rdf:first sg:person.0635706520.56
66 rdf:rest Nf3b4ce16092f48c28c3846eb9b3c47a3
67 N506ca5c50c254d1bbbfb95bb2b4e54c4 schema:name nlm_unique_id
68 schema:value 0400767
69 rdf:type schema:PropertyValue
70 N572870ef572f4b5f92c180d00ea57ad9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Congenital Abnormalities
72 rdf:type schema:DefinedTerm
73 N6a9ae8fa947d40deb1757216345ab5fb schema:issueNumber 10
74 rdf:type schema:PublicationIssue
75 N7252105e67454b008ed663b1ba671bf1 rdf:first N4356b2e30f1c41cfb7bda3fc77803581
76 rdf:rest Nb7782779739b4308b5a72e9e2787b767
77 N8b6b7331d65e4fcfb6a5da22e002f714 schema:name readcube_id
78 schema:value cbab330de4a1e694dbf394b8b262ec5d3744b20c12ff248483ea0060620646e6
79 rdf:type schema:PropertyValue
80 N9947ef8dd7c14870b7f3a0d8ec62286f rdf:first sg:person.01267211254.43
81 rdf:rest Nfd51d1820d87455284ec6d2b284e6bc1
82 N9ddc5cca3a8e41af967b68b57b30b902 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Polymerase Chain Reaction
84 rdf:type schema:DefinedTerm
85 N9ec643c1de4048efbf5fb2b509890851 rdf:first sg:person.0647760022.27
86 rdf:rest N44cc01ab7c2c4b18aa83d3b1ddc7b024
87 Naa9910a6fe2a4fa58549a180dc814f4a schema:name Historische Anthropologie und Humanökologie, Institut für Zoologie und Anthropologie, Universität Göttingen, Bürgerstrasse 50, D-37073 Göttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE
88 rdf:type schema:Organization
89 Nacaa3869035b44f7a385a04366a4fd5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Chromosomes, Human
91 rdf:type schema:DefinedTerm
92 Nb7782779739b4308b5a72e9e2787b767 rdf:first sg:person.01363262240.20
93 rdf:rest rdf:nil
94 Nbf5e1a20ee324bbfb60a0eeac9048302 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Nucleic Acid Hybridization
96 rdf:type schema:DefinedTerm
97 Nde1555456f6d468bb349f723d598f134 schema:name dimensions_id
98 schema:value pub.1042756833
99 rdf:type schema:PropertyValue
100 Ne7607d4316734bc2b7c90370cb7a3c3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Fetus
102 rdf:type schema:DefinedTerm
103 Neb296a9e35424996bd037cade6989c2e schema:name Springer Nature - SN SciGraph project
104 rdf:type schema:Organization
105 Nf3b4ce16092f48c28c3846eb9b3c47a3 rdf:first sg:person.0770722030.43
106 rdf:rest N7252105e67454b008ed663b1ba671bf1
107 Nf552ef64337646b592363591fd133f78 schema:name Historische Anthropologie und Humanökologie, Institut für Zoologie und Anthropologie, Universität Göttingen, Bürgerstrasse 50, D-37073 Göttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE
108 rdf:type schema:Organization
109 Nf82450f8ca144b188cea06a7e07fefa7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name DNA
111 rdf:type schema:DefinedTerm
112 Nfd51d1820d87455284ec6d2b284e6bc1 rdf:first sg:person.01367624564.68
113 rdf:rest N9ec643c1de4048efbf5fb2b509890851
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
118 schema:name Genetics
119 rdf:type schema:DefinedTerm
120 sg:journal.1018189 schema:issn 0028-1042
121 1432-1904
122 schema:name The Science of Nature
123 rdf:type schema:Periodical
124 sg:person.01267211254.43 schema:affiliation N3777dddd6665444cb31a4129c86326e9
125 schema:familyName Hummel
126 schema:givenName S.
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267211254.43
128 rdf:type schema:Person
129 sg:person.01363262240.20 schema:affiliation https://www.grid.ac/institutes/grid.7468.d
130 schema:familyName Tönnies
131 schema:givenName H.
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363262240.20
133 rdf:type schema:Person
134 sg:person.01367624564.68 schema:affiliation Nf552ef64337646b592363591fd133f78
135 schema:familyName Herrmann
136 schema:givenName B.
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367624564.68
138 rdf:type schema:Person
139 sg:person.0635706520.56 schema:affiliation https://www.grid.ac/institutes/grid.459629.5
140 schema:familyName Müller
141 schema:givenName D.
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635706520.56
143 rdf:type schema:Person
144 sg:person.0647760022.27 schema:affiliation Naa9910a6fe2a4fa58549a180dc814f4a
145 schema:familyName Rameckers
146 schema:givenName J.
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647760022.27
148 rdf:type schema:Person
149 sg:person.0770722030.43 schema:affiliation https://www.grid.ac/institutes/grid.7468.d
150 schema:familyName Sperling
151 schema:givenName K.
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770722030.43
153 rdf:type schema:Person
154 https://www.grid.ac/institutes/grid.459629.5 schema:alternateName Klinikum Chemnitz
155 schema:name Säuglingsklinik, Klinikum Chemnitz, Flemmingstrasse 4, D-09009 Chemnitz, Germany, DE
156 rdf:type schema:Organization
157 https://www.grid.ac/institutes/grid.7468.d schema:alternateName Humboldt University of Berlin
158 schema:name Institut für Humangenetik, Molekulare Zytogenetik, Charité, Campus Virchow Klinikum, Humboldt Universität, Haus H, Augustenburger Platz 1, D-13353 Berlin, Germany, DE
159 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...