Cytokine-facilitated priming of CD8+ T cell responses by DNA vaccination View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-02

AUTHORS

Marcin Kwissa, Andrea Kröger, Hansjörg Hauser, Jörg Reimann, Reinhold Schirmbeck

ABSTRACT

Immune responses elicited by plasmid DNA vaccination can be enhanced and modulated by codelivery of cytokine-encoding plasmids. We studied whether priming of cytotoxic T lymphocyte (CTL) responses against hepatitis B surface antigen (HBsAg) by DNA vaccines injected either intramusculary or intradermally with the gene gun is enhanced by codelivery of cytokine-encoding plasmids. From a panel of tested cytokine plasmids only mouse IFNbeta, IL-15, and GM-CSF encoding plasmids showed an effect. Intradermal gene gun vaccination with 1 micro g plasmid DNA encoding intracellular HBsAg (large LS) showed enhanced CTL priming when IFNbeta, IL15, or GM-CSF encoding plasmids were codelivered; this was not observed when a DNA vaccine encoding secreted HBsAg (small S) was injected. Intramuscular injection of low (5 micro g) doses of a DNA vaccine encoding large HBsAg did not prime CTL when delivered without cytokines, with IFNbeta or IL15-encoding plasmids. However, codelivery with GM-CSF encoding plasmid DNA primed potent, specific CTL immunity detected either in a cytotoxic assay or by determining the frequency of L(d)-restricted CD8(+) T cells specifically inducible to IFNgamma production. The codelivery of GM-CSF encoding plasmids with the DNA vaccine furthermore enhanced CTL priming to a subdominant, D(d)-restricted epitope of HBsAg. The adjuvant effect of cytokine-encoding plasmids on CTL priming by DNA vaccines is thus complex and depends on: (a) the type of cytokine (or combination of cytokines) codelivered, (b) the type (intracellular vs. secreted) and dose (1-50 micro g) of the DNA vaccine, (c) the method of DNA vaccine delivery ("naked" vs. particle-coated DNA), and (d) the (intramuscular vs. intradermal) route of delivery of the DNA vaccine. More... »

PAGES

91-101

References to SciGraph publications

Journal

TITLE

Journal of Molecular Medicine

ISSUE

2

VOLUME

81

Related Patents

  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Engineered Nucleic Acids Encoding A Modified Erythropoietin And Their Expression
  • Combination Therapy For Immunostimulation
  • Application Of Mrna For Use As A Therapeutic Agent For Tumorous Diseases
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Modified Polynucleotides Encoding Granulysin
  • Modified Polynucleotides For Treating Galactosylceramidase Protein Deficiency
  • Application Of Mrna For Use As A Therapeutic Against Tumor Diseases
  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides Encoding Septin-4
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Modified Polynucleotides For Treating Protein Deficiency
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Formulation And Delivery Of Plga Microspheres
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Compositions And Methods Of Altering Cholesterol Levels
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Proteins
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Oncology-Related Proteins And Peptides
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of G-Csf
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Terminally Modified Rna
  • Modified Polynucleotides For The Production Of Cosmetic Proteins And Peptides
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • In Vivo Production Of Proteins
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Modified Polynucleotides Encoding Hepatitis A Virus Cellular Receptor 2
  • Dlin-Kc2-Dma Lipid Nanoparticle Delivery Of Modified Polynucleotides
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Modified Polynucleotides For The Production Of Proteins
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Method For Producing A Protein Of Interest In A Primate
  • Modified Polynucleotides Encoding Aquaporin-5
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Split Dose Administration
  • Modified Polynucleotides Encoding Aryl Hydrocarbon Receptor Nuclear Translocator
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Formulation And Delivery Of Plga Microspheres
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00109-002-0395-6

    DOI

    http://dx.doi.org/10.1007/s00109-002-0395-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025225104

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12601525


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CD8-Positive T-Lymphocytes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Granulocyte-Macrophage Colony-Stimulating Factor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Interferon-beta", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Interleukin-15", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Inbred BALB C", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vaccines, DNA", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Ulm", 
              "id": "https://www.grid.ac/institutes/grid.6582.9", 
              "name": [
                "Institute for Medical Microbiology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kwissa", 
            "givenName": "Marcin", 
            "id": "sg:person.01121452067.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121452067.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "GBF German Research Centre for Biotechnology", 
              "id": "https://www.grid.ac/institutes/grid.418123.d", 
              "name": [
                "German Research Center for Biotechnology, Braunschweig, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kr\u00f6ger", 
            "givenName": "Andrea", 
            "id": "sg:person.01052563154.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01052563154.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "GBF German Research Centre for Biotechnology", 
              "id": "https://www.grid.ac/institutes/grid.418123.d", 
              "name": [
                "German Research Center for Biotechnology, Braunschweig, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hauser", 
            "givenName": "Hansj\u00f6rg", 
            "id": "sg:person.01004447754.88", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01004447754.88"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Ulm", 
              "id": "https://www.grid.ac/institutes/grid.6582.9", 
              "name": [
                "Institute for Medical Microbiology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Reimann", 
            "givenName": "J\u00f6rg", 
            "id": "sg:person.0652222532.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652222532.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Ulm", 
              "id": "https://www.grid.ac/institutes/grid.6582.9", 
              "name": [
                "Institute for Medical Microbiology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schirmbeck", 
            "givenName": "Reinhold", 
            "id": "sg:person.0625347542.58", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625347542.58"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199812)28:12<4149::aid-immu4149>3.0.co;2-d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001732440"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199812)28:12<4149::aid-immu4149>3.0.co;2-d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001732440"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-1759(96)00035-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001888887"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.186.10.1621", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003222546"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0167-5699(97)01201-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004490790"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0264-410x(00)00030-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011550921"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(88)90023-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011569095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(88)90023-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011569095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1159/000050038", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013299390"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.92.7.2889", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015066078"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1074-7613(00)80256-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021808958"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/1074-7613(95)90085-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023275782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199905)29:05<1740::aid-immu1740>3.0.co;2-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024835243"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199905)29:05<1740::aid-immu1740>3.0.co;2-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024835243"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199811)28:11<3530::aid-immu3530>3.0.co;2-c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024952415"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm0897-849", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029267096", 
              "https://doi.org/10.1038/nm0897-849"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.93.13.6291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039231731"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0264-410x(97)00036-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039736806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.93.20.10972", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040312455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0264-410x(98)00177-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041756613"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/6964", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043337681", 
              "https://doi.org/10.1038/6964"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/6964", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043337681", 
              "https://doi.org/10.1038/6964"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/icb.1997.62", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048780453", 
              "https://doi.org/10.1038/icb.1997.62"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199806)28:06<1832::aid-immu1832>3.0.co;2-j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049733339"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1521-4141(199806)28:06<1832::aid-immu1832>3.0.co;2-j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049733339"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0264-410x(00)00495-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049990154"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1074-7613(95)80001-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050289346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/cimm.1993.1169", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050787526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0264-410x(97)00302-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050867100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0966-842x(97)01145-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052609843"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1995.6.11-1447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271500"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.1996.7.13-1535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059271684"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/oli.1.1998.8.351", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059303108"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2003-02", 
        "datePublishedReg": "2003-02-01", 
        "description": "Immune responses elicited by plasmid DNA vaccination can be enhanced and modulated by codelivery of cytokine-encoding plasmids. We studied whether priming of cytotoxic T lymphocyte (CTL) responses against hepatitis B surface antigen (HBsAg) by DNA vaccines injected either intramusculary or intradermally with the gene gun is enhanced by codelivery of cytokine-encoding plasmids. From a panel of tested cytokine plasmids only mouse IFNbeta, IL-15, and GM-CSF encoding plasmids showed an effect. Intradermal gene gun vaccination with 1 micro g plasmid DNA encoding intracellular HBsAg (large LS) showed enhanced CTL priming when IFNbeta, IL15, or GM-CSF encoding plasmids were codelivered; this was not observed when a DNA vaccine encoding secreted HBsAg (small S) was injected. Intramuscular injection of low (5 micro g) doses of a DNA vaccine encoding large HBsAg did not prime CTL when delivered without cytokines, with IFNbeta or IL15-encoding plasmids. However, codelivery with GM-CSF encoding plasmid DNA primed potent, specific CTL immunity detected either in a cytotoxic assay or by determining the frequency of L(d)-restricted CD8(+) T cells specifically inducible to IFNgamma production. The codelivery of GM-CSF encoding plasmids with the DNA vaccine furthermore enhanced CTL priming to a subdominant, D(d)-restricted epitope of HBsAg. The adjuvant effect of cytokine-encoding plasmids on CTL priming by DNA vaccines is thus complex and depends on: (a) the type of cytokine (or combination of cytokines) codelivered, (b) the type (intracellular vs. secreted) and dose (1-50 micro g) of the DNA vaccine, (c) the method of DNA vaccine delivery (\"naked\" vs. particle-coated DNA), and (d) the (intramuscular vs. intradermal) route of delivery of the DNA vaccine.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00109-002-0395-6", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1057918", 
            "issn": [
              "0946-2716", 
              "1432-1440"
            ], 
            "name": "Journal of Molecular Medicine", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "81"
          }
        ], 
        "name": "Cytokine-facilitated priming of CD8+ T cell responses by DNA vaccination", 
        "pagination": "91-101", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "782c2a2706e74c058fa4cfb116337da6be8c049340c7eaa5b2622bc68d685448"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "12601525"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9504370"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00109-002-0395-6"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1025225104"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00109-002-0395-6", 
          "https://app.dimensions.ai/details/publication/pub.1025225104"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T15:43", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000481.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/s00109-002-0395-6"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00109-002-0395-6'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00109-002-0395-6'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00109-002-0395-6'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00109-002-0395-6'


     

    This table displays all metadata directly associated to this object as RDF triples.

    235 TRIPLES      21 PREDICATES      69 URIs      33 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00109-002-0395-6 schema:about N198f519dfa2c4d37a4b6a47d51ad85db
    2 N1b2e099336fc44afbd0d0e90717b83bf
    3 N52af696e48e7410989d6accfdf94f3f8
    4 N5c5f8b87452a4d0183474cf58ad5b04e
    5 N6862d36feb824c48b85f700e2c093122
    6 N91fb401981fb468d939fb3d58aeffcb6
    7 Naabcc68eeed14098ab3ffb72fe8fa603
    8 Nbea2841e39a64d8995434f419373f78b
    9 Nd9a610c38eb64bbc873df84c4181e780
    10 Nee6de3e6f19a47ce9090e2e5ebf5a223
    11 Nf46c2f377d4942de9aa2c0adcbd2190d
    12 Nf5a82348cf174f3e9f8666f635218e7b
    13 anzsrc-for:11
    14 anzsrc-for:1107
    15 schema:author Nc6f1ab495625485ba94480b47a0d072b
    16 schema:citation sg:pub.10.1038/6964
    17 sg:pub.10.1038/icb.1997.62
    18 sg:pub.10.1038/nm0897-849
    19 https://doi.org/10.1002/(sici)1521-4141(199806)28:06<1832::aid-immu1832>3.0.co;2-j
    20 https://doi.org/10.1002/(sici)1521-4141(199811)28:11<3530::aid-immu3530>3.0.co;2-c
    21 https://doi.org/10.1002/(sici)1521-4141(199812)28:12<4149::aid-immu4149>3.0.co;2-d
    22 https://doi.org/10.1002/(sici)1521-4141(199905)29:05<1740::aid-immu1740>3.0.co;2-x
    23 https://doi.org/10.1006/cimm.1993.1169
    24 https://doi.org/10.1016/0022-1759(96)00035-x
    25 https://doi.org/10.1016/0378-1119(88)90023-6
    26 https://doi.org/10.1016/1074-7613(95)90085-3
    27 https://doi.org/10.1016/s0167-5699(97)01201-2
    28 https://doi.org/10.1016/s0264-410x(00)00030-x
    29 https://doi.org/10.1016/s0264-410x(00)00495-3
    30 https://doi.org/10.1016/s0264-410x(97)00036-4
    31 https://doi.org/10.1016/s0264-410x(97)00302-2
    32 https://doi.org/10.1016/s0264-410x(98)00177-7
    33 https://doi.org/10.1016/s0966-842x(97)01145-1
    34 https://doi.org/10.1016/s1074-7613(00)80256-3
    35 https://doi.org/10.1016/s1074-7613(95)80001-8
    36 https://doi.org/10.1073/pnas.92.7.2889
    37 https://doi.org/10.1073/pnas.93.13.6291
    38 https://doi.org/10.1073/pnas.93.20.10972
    39 https://doi.org/10.1084/jem.186.10.1621
    40 https://doi.org/10.1089/hum.1995.6.11-1447
    41 https://doi.org/10.1089/hum.1996.7.13-1535
    42 https://doi.org/10.1089/oli.1.1998.8.351
    43 https://doi.org/10.1159/000050038
    44 schema:datePublished 2003-02
    45 schema:datePublishedReg 2003-02-01
    46 schema:description Immune responses elicited by plasmid DNA vaccination can be enhanced and modulated by codelivery of cytokine-encoding plasmids. We studied whether priming of cytotoxic T lymphocyte (CTL) responses against hepatitis B surface antigen (HBsAg) by DNA vaccines injected either intramusculary or intradermally with the gene gun is enhanced by codelivery of cytokine-encoding plasmids. From a panel of tested cytokine plasmids only mouse IFNbeta, IL-15, and GM-CSF encoding plasmids showed an effect. Intradermal gene gun vaccination with 1 micro g plasmid DNA encoding intracellular HBsAg (large LS) showed enhanced CTL priming when IFNbeta, IL15, or GM-CSF encoding plasmids were codelivered; this was not observed when a DNA vaccine encoding secreted HBsAg (small S) was injected. Intramuscular injection of low (5 micro g) doses of a DNA vaccine encoding large HBsAg did not prime CTL when delivered without cytokines, with IFNbeta or IL15-encoding plasmids. However, codelivery with GM-CSF encoding plasmid DNA primed potent, specific CTL immunity detected either in a cytotoxic assay or by determining the frequency of L(d)-restricted CD8(+) T cells specifically inducible to IFNgamma production. The codelivery of GM-CSF encoding plasmids with the DNA vaccine furthermore enhanced CTL priming to a subdominant, D(d)-restricted epitope of HBsAg. The adjuvant effect of cytokine-encoding plasmids on CTL priming by DNA vaccines is thus complex and depends on: (a) the type of cytokine (or combination of cytokines) codelivered, (b) the type (intracellular vs. secreted) and dose (1-50 micro g) of the DNA vaccine, (c) the method of DNA vaccine delivery ("naked" vs. particle-coated DNA), and (d) the (intramuscular vs. intradermal) route of delivery of the DNA vaccine.
    47 schema:genre research_article
    48 schema:inLanguage en
    49 schema:isAccessibleForFree false
    50 schema:isPartOf N44754d24347f47a48114b7791635a7b8
    51 Nc365e7cd30204bbea60aff35d6181691
    52 sg:journal.1057918
    53 schema:name Cytokine-facilitated priming of CD8+ T cell responses by DNA vaccination
    54 schema:pagination 91-101
    55 schema:productId N1a855ee2c59e4f4fba86eeca7ab1f34d
    56 N1f36abb02b14421faf3a1e5e8a985f1c
    57 N2d56911043f94ed7b7d189611745c570
    58 N9387467ec06b4814b4e3420adb3a5b57
    59 Na1dde2de61294e87830fba2f0f78f082
    60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025225104
    61 https://doi.org/10.1007/s00109-002-0395-6
    62 schema:sdDatePublished 2019-04-10T15:43
    63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    64 schema:sdPublisher N0bf13122db4d4b62be21cece2d13739c
    65 schema:url http://link.springer.com/10.1007/s00109-002-0395-6
    66 sgo:license sg:explorer/license/
    67 sgo:sdDataset articles
    68 rdf:type schema:ScholarlyArticle
    69 N0bf13122db4d4b62be21cece2d13739c schema:name Springer Nature - SN SciGraph project
    70 rdf:type schema:Organization
    71 N1096cb2b3c484e53a4ee63e3964773e1 rdf:first sg:person.0625347542.58
    72 rdf:rest rdf:nil
    73 N198f519dfa2c4d37a4b6a47d51ad85db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Plasmids
    75 rdf:type schema:DefinedTerm
    76 N1a855ee2c59e4f4fba86eeca7ab1f34d schema:name pubmed_id
    77 schema:value 12601525
    78 rdf:type schema:PropertyValue
    79 N1b2e099336fc44afbd0d0e90717b83bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Animals
    81 rdf:type schema:DefinedTerm
    82 N1f36abb02b14421faf3a1e5e8a985f1c schema:name readcube_id
    83 schema:value 782c2a2706e74c058fa4cfb116337da6be8c049340c7eaa5b2622bc68d685448
    84 rdf:type schema:PropertyValue
    85 N2d56911043f94ed7b7d189611745c570 schema:name nlm_unique_id
    86 schema:value 9504370
    87 rdf:type schema:PropertyValue
    88 N44754d24347f47a48114b7791635a7b8 schema:volumeNumber 81
    89 rdf:type schema:PublicationVolume
    90 N4c30e6b235c44453b850d5212de5ac58 rdf:first sg:person.0652222532.01
    91 rdf:rest N1096cb2b3c484e53a4ee63e3964773e1
    92 N52af696e48e7410989d6accfdf94f3f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Interleukin-15
    94 rdf:type schema:DefinedTerm
    95 N5c5f8b87452a4d0183474cf58ad5b04e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Mice, Inbred BALB C
    97 rdf:type schema:DefinedTerm
    98 N6862d36feb824c48b85f700e2c093122 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Female
    100 rdf:type schema:DefinedTerm
    101 N91fb401981fb468d939fb3d58aeffcb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Genetic Vectors
    103 rdf:type schema:DefinedTerm
    104 N9387467ec06b4814b4e3420adb3a5b57 schema:name dimensions_id
    105 schema:value pub.1025225104
    106 rdf:type schema:PropertyValue
    107 N976ac3a0837e47b381d29aecbf203406 rdf:first sg:person.01004447754.88
    108 rdf:rest N4c30e6b235c44453b850d5212de5ac58
    109 Na1dde2de61294e87830fba2f0f78f082 schema:name doi
    110 schema:value 10.1007/s00109-002-0395-6
    111 rdf:type schema:PropertyValue
    112 Naabcc68eeed14098ab3ffb72fe8fa603 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name CD8-Positive T-Lymphocytes
    114 rdf:type schema:DefinedTerm
    115 Nbea2841e39a64d8995434f419373f78b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Gene Transfer Techniques
    117 rdf:type schema:DefinedTerm
    118 Nc365e7cd30204bbea60aff35d6181691 schema:issueNumber 2
    119 rdf:type schema:PublicationIssue
    120 Nc6f1ab495625485ba94480b47a0d072b rdf:first sg:person.01121452067.75
    121 rdf:rest Nfdae4881fd1f4fba8aa44e165a01c1e0
    122 Nd9a610c38eb64bbc873df84c4181e780 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Mice
    124 rdf:type schema:DefinedTerm
    125 Nee6de3e6f19a47ce9090e2e5ebf5a223 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Interferon-beta
    127 rdf:type schema:DefinedTerm
    128 Nf46c2f377d4942de9aa2c0adcbd2190d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Granulocyte-Macrophage Colony-Stimulating Factor
    130 rdf:type schema:DefinedTerm
    131 Nf5a82348cf174f3e9f8666f635218e7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Vaccines, DNA
    133 rdf:type schema:DefinedTerm
    134 Nfdae4881fd1f4fba8aa44e165a01c1e0 rdf:first sg:person.01052563154.39
    135 rdf:rest N976ac3a0837e47b381d29aecbf203406
    136 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    137 schema:name Medical and Health Sciences
    138 rdf:type schema:DefinedTerm
    139 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    140 schema:name Immunology
    141 rdf:type schema:DefinedTerm
    142 sg:journal.1057918 schema:issn 0946-2716
    143 1432-1440
    144 schema:name Journal of Molecular Medicine
    145 rdf:type schema:Periodical
    146 sg:person.01004447754.88 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
    147 schema:familyName Hauser
    148 schema:givenName Hansjörg
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01004447754.88
    150 rdf:type schema:Person
    151 sg:person.01052563154.39 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
    152 schema:familyName Kröger
    153 schema:givenName Andrea
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01052563154.39
    155 rdf:type schema:Person
    156 sg:person.01121452067.75 schema:affiliation https://www.grid.ac/institutes/grid.6582.9
    157 schema:familyName Kwissa
    158 schema:givenName Marcin
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121452067.75
    160 rdf:type schema:Person
    161 sg:person.0625347542.58 schema:affiliation https://www.grid.ac/institutes/grid.6582.9
    162 schema:familyName Schirmbeck
    163 schema:givenName Reinhold
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625347542.58
    165 rdf:type schema:Person
    166 sg:person.0652222532.01 schema:affiliation https://www.grid.ac/institutes/grid.6582.9
    167 schema:familyName Reimann
    168 schema:givenName Jörg
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652222532.01
    170 rdf:type schema:Person
    171 sg:pub.10.1038/6964 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043337681
    172 https://doi.org/10.1038/6964
    173 rdf:type schema:CreativeWork
    174 sg:pub.10.1038/icb.1997.62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048780453
    175 https://doi.org/10.1038/icb.1997.62
    176 rdf:type schema:CreativeWork
    177 sg:pub.10.1038/nm0897-849 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029267096
    178 https://doi.org/10.1038/nm0897-849
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1002/(sici)1521-4141(199806)28:06<1832::aid-immu1832>3.0.co;2-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1049733339
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1002/(sici)1521-4141(199811)28:11<3530::aid-immu3530>3.0.co;2-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1024952415
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1002/(sici)1521-4141(199812)28:12<4149::aid-immu4149>3.0.co;2-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1001732440
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1002/(sici)1521-4141(199905)29:05<1740::aid-immu1740>3.0.co;2-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024835243
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1006/cimm.1993.1169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050787526
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1016/0022-1759(96)00035-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1001888887
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1016/0378-1119(88)90023-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011569095
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1016/1074-7613(95)90085-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023275782
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1016/s0167-5699(97)01201-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004490790
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1016/s0264-410x(00)00030-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011550921
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1016/s0264-410x(00)00495-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049990154
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1016/s0264-410x(97)00036-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039736806
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1016/s0264-410x(97)00302-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050867100
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1016/s0264-410x(98)00177-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041756613
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1016/s0966-842x(97)01145-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052609843
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/s1074-7613(00)80256-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021808958
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/s1074-7613(95)80001-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050289346
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1073/pnas.92.7.2889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015066078
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1073/pnas.93.13.6291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039231731
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1073/pnas.93.20.10972 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040312455
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1084/jem.186.10.1621 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003222546
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1089/hum.1995.6.11-1447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271500
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1089/hum.1996.7.13-1535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271684
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1089/oli.1.1998.8.351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059303108
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1159/000050038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013299390
    229 rdf:type schema:CreativeWork
    230 https://www.grid.ac/institutes/grid.418123.d schema:alternateName GBF German Research Centre for Biotechnology
    231 schema:name German Research Center for Biotechnology, Braunschweig, Germany
    232 rdf:type schema:Organization
    233 https://www.grid.ac/institutes/grid.6582.9 schema:alternateName University of Ulm
    234 schema:name Institute for Medical Microbiology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany
    235 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...