Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-09-06

AUTHORS

Akhilesh K. Yadav, N. Manika, Guru D. Bagchi, Madan M. Gupta

ABSTRACT

A reverse-phase liquid chromatographic method has been established for analysis of oroxylin A, chrysin, baicalein, and hispidulin in the roots of Oroxylum indicum (Bignoneaceae). Under the optimized conditions, well-resolved separation of pure compounds with significantly different Rt values was achieved on chromolith C18 column (100 mm × 4.6 mm, 10 μm) using isocratic elution of acetonitrile and water (0.1 % trifluoroacetic acid); with acceptable validation results such as linearity, sensitivity, and recovery in terms of %RSD. The calibration curves were linear in the concentration range of 200–500 ng ml−1. Method was validated as per ICH guidelines. The developed reproducible and robust method may be applied for the quality assays of O. indicum and phytopreparations. More... »

PAGES

2222-2227

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00044-012-0214-8

DOI

http://dx.doi.org/10.1007/s00044-012-0214-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052376602


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Analytical Chemistry", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, 226 015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, 226 015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yadav", 
        "givenName": "Akhilesh K.", 
        "id": "sg:person.0713640055.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713640055.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Manika", 
        "givenName": "N.", 
        "id": "sg:person.012267536453.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012267536453.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bagchi", 
        "givenName": "Guru D.", 
        "id": "sg:person.012562302341.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012562302341.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, 226 015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, 226 015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gupta", 
        "givenName": "Madan M.", 
        "id": "sg:person.01242460441.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-3-642-88458-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035887237", 
          "https://doi.org/10.1007/978-3-642-88458-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1365/s10337-009-0963-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007868757", 
          "https://doi.org/10.1365/s10337-009-0963-9"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-09-06", 
    "datePublishedReg": "2012-09-06", 
    "description": "A reverse-phase liquid chromatographic method has been established for analysis of oroxylin A, chrysin, baicalein, and hispidulin in the roots of Oroxylum indicum (Bignoneaceae). Under the optimized conditions, well-resolved separation of pure compounds with significantly different Rt values was achieved on chromolith C18 column (100\u00a0mm\u00a0\u00d7\u00a04.6\u00a0mm, 10\u00a0\u03bcm) using isocratic elution of acetonitrile and water (0.1\u00a0% trifluoroacetic acid); with acceptable validation results such as linearity, sensitivity, and recovery in terms of %RSD. The calibration curves were linear in the concentration range of 200\u2013500\u00a0ng\u00a0ml\u22121. Method was validated as per ICH guidelines. The developed reproducible and robust method may be applied for the quality assays of O. indicum and phytopreparations.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00044-012-0214-8", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1102690", 
        "issn": [
          "1054-2523", 
          "1554-8120"
        ], 
        "name": "Medicinal Chemistry Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "22"
      }
    ], 
    "keywords": [
      "different Rt values", 
      "acceptable validation results", 
      "Chromolith C18 column", 
      "reversed-phase liquid chromatographic method", 
      "validation results", 
      "ICH guidelines", 
      "RT values", 
      "robust method", 
      "method", 
      "calibration curve", 
      "liquid chromatographic method", 
      "concentration range", 
      "water", 
      "linearity", 
      "separation", 
      "column", 
      "C18 column", 
      "range", 
      "conditions", 
      "RSD", 
      "simultaneous determination", 
      "isocratic elution", 
      "curves", 
      "chromatographic method", 
      "recovery", 
      "results", 
      "values", 
      "determination", 
      "terms", 
      "sensitivity", 
      "analysis", 
      "elution", 
      "acetonitrile", 
      "pure compounds", 
      "compounds", 
      "RP-HPLC", 
      "quality assays", 
      "guidelines", 
      "roots", 
      "Oroxylum indicum", 
      "indicum", 
      "phytopreparations", 
      "flavonoids", 
      "baicalein", 
      "assays", 
      "chrysin", 
      "oroxylin A", 
      "hispidulin"
    ], 
    "name": "Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC", 
    "pagination": "2222-2227", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1052376602"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00044-012-0214-8"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00044-012-0214-8", 
      "https://app.dimensions.ai/details/publication/pub.1052376602"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_574.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00044-012-0214-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00044-012-0214-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00044-012-0214-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00044-012-0214-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00044-012-0214-8'


 

This table displays all metadata directly associated to this object as RDF triples.

137 TRIPLES      22 PREDICATES      75 URIs      65 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00044-012-0214-8 schema:about anzsrc-for:03
2 anzsrc-for:0301
3 schema:author N3c216808266244048a4928b74a27bda5
4 schema:citation sg:pub.10.1007/978-3-642-88458-0
5 sg:pub.10.1365/s10337-009-0963-9
6 schema:datePublished 2012-09-06
7 schema:datePublishedReg 2012-09-06
8 schema:description A reverse-phase liquid chromatographic method has been established for analysis of oroxylin A, chrysin, baicalein, and hispidulin in the roots of Oroxylum indicum (Bignoneaceae). Under the optimized conditions, well-resolved separation of pure compounds with significantly different Rt values was achieved on chromolith C18 column (100 mm × 4.6 mm, 10 μm) using isocratic elution of acetonitrile and water (0.1 % trifluoroacetic acid); with acceptable validation results such as linearity, sensitivity, and recovery in terms of %RSD. The calibration curves were linear in the concentration range of 200–500 ng ml−1. Method was validated as per ICH guidelines. The developed reproducible and robust method may be applied for the quality assays of O. indicum and phytopreparations.
9 schema:genre article
10 schema:inLanguage en
11 schema:isAccessibleForFree false
12 schema:isPartOf N72b39601bb2c442b8473ed4f7a78d6f3
13 Nb59a7b06a16b4fc3aba4f0bdcbbab7d1
14 sg:journal.1102690
15 schema:keywords C18 column
16 Chromolith C18 column
17 ICH guidelines
18 Oroxylum indicum
19 RP-HPLC
20 RSD
21 RT values
22 acceptable validation results
23 acetonitrile
24 analysis
25 assays
26 baicalein
27 calibration curve
28 chromatographic method
29 chrysin
30 column
31 compounds
32 concentration range
33 conditions
34 curves
35 determination
36 different Rt values
37 elution
38 flavonoids
39 guidelines
40 hispidulin
41 indicum
42 isocratic elution
43 linearity
44 liquid chromatographic method
45 method
46 oroxylin A
47 phytopreparations
48 pure compounds
49 quality assays
50 range
51 recovery
52 results
53 reversed-phase liquid chromatographic method
54 robust method
55 roots
56 sensitivity
57 separation
58 simultaneous determination
59 terms
60 validation results
61 values
62 water
63 schema:name Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC
64 schema:pagination 2222-2227
65 schema:productId N828cdae66fc844b3a254cdb9f300c2e2
66 Nce73fa8bbd1d48069dd6edce9527cd7f
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052376602
68 https://doi.org/10.1007/s00044-012-0214-8
69 schema:sdDatePublished 2022-05-20T07:28
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher Nf8072264ae9740428bac65ce19ec5a60
72 schema:url https://doi.org/10.1007/s00044-012-0214-8
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N05d04a8c23c14e77be5d86ad4e5f6254 rdf:first sg:person.01242460441.07
77 rdf:rest rdf:nil
78 N3c216808266244048a4928b74a27bda5 rdf:first sg:person.0713640055.22
79 rdf:rest N5c07feb104924f2c889d8c69b38a46cd
80 N5c07feb104924f2c889d8c69b38a46cd rdf:first sg:person.012267536453.48
81 rdf:rest Neb035c4c045e4dd58b8ba1a9e5c26d00
82 N72b39601bb2c442b8473ed4f7a78d6f3 schema:volumeNumber 22
83 rdf:type schema:PublicationVolume
84 N828cdae66fc844b3a254cdb9f300c2e2 schema:name doi
85 schema:value 10.1007/s00044-012-0214-8
86 rdf:type schema:PropertyValue
87 Nb59a7b06a16b4fc3aba4f0bdcbbab7d1 schema:issueNumber 5
88 rdf:type schema:PublicationIssue
89 Nce73fa8bbd1d48069dd6edce9527cd7f schema:name dimensions_id
90 schema:value pub.1052376602
91 rdf:type schema:PropertyValue
92 Neb035c4c045e4dd58b8ba1a9e5c26d00 rdf:first sg:person.012562302341.79
93 rdf:rest N05d04a8c23c14e77be5d86ad4e5f6254
94 Nf8072264ae9740428bac65ce19ec5a60 schema:name Springer Nature - SN SciGraph project
95 rdf:type schema:Organization
96 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
97 schema:name Chemical Sciences
98 rdf:type schema:DefinedTerm
99 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
100 schema:name Analytical Chemistry
101 rdf:type schema:DefinedTerm
102 sg:journal.1102690 schema:issn 1054-2523
103 1554-8120
104 schema:name Medicinal Chemistry Research
105 schema:publisher Springer Nature
106 rdf:type schema:Periodical
107 sg:person.012267536453.48 schema:affiliation grid-institutes:grid.417631.6
108 schema:familyName Manika
109 schema:givenName N.
110 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012267536453.48
111 rdf:type schema:Person
112 sg:person.01242460441.07 schema:affiliation grid-institutes:grid.417631.6
113 schema:familyName Gupta
114 schema:givenName Madan M.
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07
116 rdf:type schema:Person
117 sg:person.012562302341.79 schema:affiliation grid-institutes:grid.417631.6
118 schema:familyName Bagchi
119 schema:givenName Guru D.
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012562302341.79
121 rdf:type schema:Person
122 sg:person.0713640055.22 schema:affiliation grid-institutes:grid.417631.6
123 schema:familyName Yadav
124 schema:givenName Akhilesh K.
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713640055.22
126 rdf:type schema:Person
127 sg:pub.10.1007/978-3-642-88458-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035887237
128 https://doi.org/10.1007/978-3-642-88458-0
129 rdf:type schema:CreativeWork
130 sg:pub.10.1365/s10337-009-0963-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007868757
131 https://doi.org/10.1365/s10337-009-0963-9
132 rdf:type schema:CreativeWork
133 grid-institutes:grid.417631.6 schema:alternateName Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, 226 015, Lucknow, India
134 Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India
135 schema:name Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, 226 015, Lucknow, India
136 Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India
137 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...