Potentiation of a neuronal nicotinic receptor via pseudo-agonist site View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03

AUTHORS

Simone Mazzaferro, Isabel Bermudez, Steven M. Sine

ABSTRACT

Neuronal nicotinic receptors containing α4 and β2 subunits assemble in two pentameric stoichiometries, (α4)3(β2)2 and (α4)2(β2)3, each with distinct pharmacological signatures; (α4)3(β2)2 receptors are strongly potentiated by the drug NS9283, whereas (α4)2(β2)3 receptors are unaffected. Despite this stoichiometry-selective pharmacology, the molecular identity of the target for NS9283 remains elusive. Here, studying (α4)3(β2)2 receptors, we show that mutations at either the principal face of the β2 subunit or the complementary face of the α4 subunit prevent NS9283 potentiation of ACh-elicited single-channel currents, suggesting the drug targets the β2-α4 pseudo-agonist sites, the α4-α4 agonist site, or both sites. To distinguish among these possibilities, we generated concatemeric receptors with mutations at specified subunit interfaces, and monitored the ability of NS9283 to potentiate ACh-elicited single-channel currents. We find that a mutation at the principal face of the β2 subunit at either β2-α4 pseudo-agonist site suppresses potentiation, whereas mutation at the complementary face of the α4 subunit at the α4-α4 agonist site allows a significant potentiation. Thus, monitoring potentiation of single concatemeric receptor channels reveals that the β2-α4 pseudo-agonist sites are required for stoichiometry-selective drug action. Together with the recently determined structure of the (α4)3(β2)2 receptor, the findings have implications for structure-guided drug design. More... »

PAGES

1151-1167

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00018-018-2993-7

DOI

http://dx.doi.org/10.1007/s00018-018-2993-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111012116

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30600358


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetylcholine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Action Potentials", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Synergism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HEK293 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nicotinic Agonists", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxadiazoles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Isoforms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Subunits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pyridines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Nicotinic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Mayo Clinic", 
          "id": "https://www.grid.ac/institutes/grid.66875.3a", 
          "name": [
            "Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mazzaferro", 
        "givenName": "Simone", 
        "id": "sg:person.01277141022.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277141022.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Oxford Brookes University", 
          "id": "https://www.grid.ac/institutes/grid.7628.b", 
          "name": [
            "School of Life Sciences, Oxford Brookes University, OX3 OBP, Oxford, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bermudez", 
        "givenName": "Isabel", 
        "id": "sg:person.01332247171.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332247171.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Mayo Clinic", 
          "id": "https://www.grid.ac/institutes/grid.66875.3a", 
          "name": [
            "Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA", 
            "Department of Neurology, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA", 
            "Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sine", 
        "givenName": "Steven M.", 
        "id": "sg:person.012453140337.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012453140337.73"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m111.262014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004137052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.96.2.395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005170727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00213-016-4475-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005498689", 
          "https://doi.org/10.1007/s00213-016-4475-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00213-016-4475-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005498689", 
          "https://doi.org/10.1007/s00213-016-4475-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00213-016-4475-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005498689", 
          "https://doi.org/10.1007/s00213-016-4475-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-7858-1_11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007617656", 
          "https://doi.org/10.1007/978-1-4615-7858-1_11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pharmthera.2012.08.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007973268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.20.9436", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010448395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1301909110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012356886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/19756", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013295470", 
          "https://doi.org/10.1038/19756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/19756", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013295470", 
          "https://doi.org/10.1038/19756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(94)90424-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016705074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/374065a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016922165", 
          "https://doi.org/10.1038/374065a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/jpet.113.208389", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017548461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1476-5381.2012.01989.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020193118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.38.27145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022242550"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0165-6147(97)90675-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024388735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m011549200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024443795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn1298", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028421274", 
          "https://doi.org/10.1038/nrn1298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn1298", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028421274", 
          "https://doi.org/10.1038/nrn1298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.r112.386664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029615124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2011.05.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031079549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m114.555136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031514339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2006.03915.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031708835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physrev.00015.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033465396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0804372105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034495968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.105.015438", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035023675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.18.8392", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035790995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature19785", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036145124", 
          "https://doi.org/10.1038/nature19785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.111.076059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036412441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.1228-08.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036431366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2004.12.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036771134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/jpet.115.223933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040568779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.201611584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041251576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.106.023044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043350094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuropharm.2008.07.041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043666130"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/cshperspect.a012112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044197018"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/bph.12095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044664390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.63.2.332", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044839487"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.0448-08.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044970751"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m113.498618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045269703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1476-5381.2008.00104.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046674097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/bph.12268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047294556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd2927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049839544", 
          "https://doi.org/10.1038/nrd2927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd2927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049839544", 
          "https://doi.org/10.1038/nrd2927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m114.568097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050779471"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(87)83298-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050989941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.1859-09.2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051026320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m115.684373", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058213963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m116.751206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058215530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m116.764183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058215788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/156802611794863562", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069194065"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.20-17-06431.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074691708"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082666162", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.18-12-04461.1998", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083273115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.54.6.1124", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083362538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m116.771246", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085276787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/bph.13905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085968495"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/bph.13905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085968495"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.116.106880", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090613954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.201711846", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100715462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41586-018-0081-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103702825", 
          "https://doi.org/10.1038/s41586-018-0081-7"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-03", 
    "datePublishedReg": "2019-03-01", 
    "description": "Neuronal nicotinic receptors containing \u03b14 and \u03b22 subunits assemble in two pentameric stoichiometries, (\u03b14)3(\u03b22)2 and (\u03b14)2(\u03b22)3, each with distinct pharmacological signatures; (\u03b14)3(\u03b22)2 receptors are strongly potentiated by the drug NS9283, whereas (\u03b14)2(\u03b22)3 receptors are unaffected. Despite this stoichiometry-selective pharmacology, the molecular identity of the target for NS9283 remains elusive. Here, studying (\u03b14)3(\u03b22)2 receptors, we show that mutations at either the principal face of the \u03b22 subunit or the complementary face of the \u03b14 subunit prevent NS9283 potentiation of ACh-elicited single-channel currents, suggesting the drug targets the \u03b22-\u03b14 pseudo-agonist sites, the \u03b14-\u03b14 agonist site, or both sites. To distinguish among these possibilities, we generated concatemeric receptors with mutations at specified subunit interfaces, and monitored the ability of NS9283 to potentiate ACh-elicited single-channel currents. We find that a mutation at the principal face of the \u03b22 subunit at either \u03b22-\u03b14 pseudo-agonist site suppresses potentiation, whereas mutation at the complementary face of the \u03b14 subunit at the \u03b14-\u03b14 agonist site allows a significant potentiation. Thus, monitoring potentiation of single concatemeric receptor channels reveals that the \u03b22-\u03b14 pseudo-agonist sites are required for stoichiometry-selective drug action. Together with the recently determined structure of the (\u03b14)3(\u03b22)2 receptor, the findings have implications for structure-guided drug design.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00018-018-2993-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2557573", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1295005", 
        "issn": [
          "1420-682X", 
          "1420-9071"
        ], 
        "name": "Cellular and Molecular Life Sciences", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "76"
      }
    ], 
    "name": "Potentiation of a neuronal nicotinic receptor via pseudo-agonist site", 
    "pagination": "1151-1167", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "105aaee2904c9fdc9ed4f268a2b4d52e4c267b740bc7fc2509c2c2d6633b8954"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30600358"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9705402"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00018-018-2993-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111012116"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00018-018-2993-7", 
      "https://app.dimensions.ai/details/publication/pub.1111012116"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78972_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00018-018-2993-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00018-018-2993-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00018-018-2993-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00018-018-2993-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00018-018-2993-7'


 

This table displays all metadata directly associated to this object as RDF triples.

329 TRIPLES      21 PREDICATES      101 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00018-018-2993-7 schema:about N19874f210b5d40bdbce906c24b7e8179
2 N2d960355393a44f89696a9f9850a5a9f
3 N52e0908876c24ddc9b897cb1243ce531
4 N5e827e0b65844340bb3adbfe3d47ac97
5 N92c6e30f64404fa9a86940fe582a47ac
6 N9d7329a0ed7f48bda48e78351da5963d
7 Na0005b18daa745e4ba7301f2bac08bca
8 Nafb1e1f88f1f42b2a4aab05ef7650e67
9 Nb40f164bfeca4a379681462169c169ed
10 Nc5b8a19fa73e47778d36a565cc5b71a4
11 Ncf0d06674b3740b6be5496f85fa8d361
12 Nd7bfb9a8f402408983ece02534271d31
13 Nd94b29461c5a42caa501b44ace367578
14 Ne1507c8fd12e4a7f93b3f9407d3444af
15 Nee6013dbb2064a08aa142fbd457d73aa
16 Nf6dde0011bc04b1484212bb543ffe410
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author N89bd54daa3de4c9a84bb52359c4dd513
20 schema:citation sg:pub.10.1007/978-1-4615-7858-1_11
21 sg:pub.10.1007/s00213-016-4475-7
22 sg:pub.10.1038/19756
23 sg:pub.10.1038/374065a0
24 sg:pub.10.1038/nature19785
25 sg:pub.10.1038/nrd2927
26 sg:pub.10.1038/nrn1298
27 sg:pub.10.1038/s41586-018-0081-7
28 https://app.dimensions.ai/details/publication/pub.1082666162
29 https://doi.org/10.1016/0896-6273(94)90424-3
30 https://doi.org/10.1016/j.bcp.2011.05.007
31 https://doi.org/10.1016/j.jmb.2004.12.031
32 https://doi.org/10.1016/j.neuropharm.2008.07.041
33 https://doi.org/10.1016/j.pharmthera.2012.08.012
34 https://doi.org/10.1016/s0006-3495(87)83298-8
35 https://doi.org/10.1016/s0165-6147(97)90675-1
36 https://doi.org/10.1073/pnas.0804372105
37 https://doi.org/10.1073/pnas.1301909110
38 https://doi.org/10.1073/pnas.90.18.8392
39 https://doi.org/10.1073/pnas.90.20.9436
40 https://doi.org/10.1074/jbc.274.38.27145
41 https://doi.org/10.1074/jbc.m011549200
42 https://doi.org/10.1074/jbc.m111.262014
43 https://doi.org/10.1074/jbc.m113.498618
44 https://doi.org/10.1074/jbc.m114.555136
45 https://doi.org/10.1074/jbc.m114.568097
46 https://doi.org/10.1074/jbc.m115.684373
47 https://doi.org/10.1074/jbc.m116.751206
48 https://doi.org/10.1074/jbc.m116.764183
49 https://doi.org/10.1074/jbc.m116.771246
50 https://doi.org/10.1074/jbc.r112.386664
51 https://doi.org/10.1085/jgp.201611584
52 https://doi.org/10.1085/jgp.201711846
53 https://doi.org/10.1085/jgp.96.2.395
54 https://doi.org/10.1101/cshperspect.a012112
55 https://doi.org/10.1111/bph.12095
56 https://doi.org/10.1111/bph.12268
57 https://doi.org/10.1111/bph.13905
58 https://doi.org/10.1111/j.1471-4159.2006.03915.x
59 https://doi.org/10.1111/j.1476-5381.2008.00104.x
60 https://doi.org/10.1111/j.1476-5381.2012.01989.x
61 https://doi.org/10.1124/jpet.113.208389
62 https://doi.org/10.1124/jpet.115.223933
63 https://doi.org/10.1124/mol.105.015438
64 https://doi.org/10.1124/mol.106.023044
65 https://doi.org/10.1124/mol.111.076059
66 https://doi.org/10.1124/mol.116.106880
67 https://doi.org/10.1124/mol.54.6.1124
68 https://doi.org/10.1124/mol.63.2.332
69 https://doi.org/10.1152/physrev.00015.2008
70 https://doi.org/10.1523/jneurosci.0448-08.2008
71 https://doi.org/10.1523/jneurosci.1228-08.2008
72 https://doi.org/10.1523/jneurosci.18-12-04461.1998
73 https://doi.org/10.1523/jneurosci.1859-09.2009
74 https://doi.org/10.1523/jneurosci.20-17-06431.2000
75 https://doi.org/10.2174/156802611794863562
76 schema:datePublished 2019-03
77 schema:datePublishedReg 2019-03-01
78 schema:description Neuronal nicotinic receptors containing α4 and β2 subunits assemble in two pentameric stoichiometries, (α4)<sub>3</sub>(β2)<sub>2</sub> and (α4)<sub>2</sub>(β2)<sub>3</sub>, each with distinct pharmacological signatures; (α4)<sub>3</sub>(β2)<sub>2</sub> receptors are strongly potentiated by the drug NS9283, whereas (α4)<sub>2</sub>(β2)<sub>3</sub> receptors are unaffected. Despite this stoichiometry-selective pharmacology, the molecular identity of the target for NS9283 remains elusive. Here, studying (α4)<sub>3</sub>(β2)<sub>2</sub> receptors, we show that mutations at either the principal face of the β2 subunit or the complementary face of the α4 subunit prevent NS9283 potentiation of ACh-elicited single-channel currents, suggesting the drug targets the β2-α4 pseudo-agonist sites, the α4-α4 agonist site, or both sites. To distinguish among these possibilities, we generated concatemeric receptors with mutations at specified subunit interfaces, and monitored the ability of NS9283 to potentiate ACh-elicited single-channel currents. We find that a mutation at the principal face of the β2 subunit at either β2-α4 pseudo-agonist site suppresses potentiation, whereas mutation at the complementary face of the α4 subunit at the α4-α4 agonist site allows a significant potentiation. Thus, monitoring potentiation of single concatemeric receptor channels reveals that the β2-α4 pseudo-agonist sites are required for stoichiometry-selective drug action. Together with the recently determined structure of the (α4)<sub>3</sub>(β2)<sub>2</sub> receptor, the findings have implications for structure-guided drug design.
79 schema:genre research_article
80 schema:inLanguage en
81 schema:isAccessibleForFree false
82 schema:isPartOf N51439aad386a44d9a7e481f9826c5abf
83 N6c6a97622431458296d6e407a2346d62
84 sg:journal.1295005
85 schema:name Potentiation of a neuronal nicotinic receptor via pseudo-agonist site
86 schema:pagination 1151-1167
87 schema:productId N7153aabf70d14e5f84aa48929dafb0f3
88 N71ba4444662e4ae09379992d4ae2953e
89 N79e3c20a261c4f0bababf61c79493685
90 N7d8b6097434f4089b50aa1240ca0a125
91 Neb0efeb7b198469e89934ef2fbe69e4b
92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111012116
93 https://doi.org/10.1007/s00018-018-2993-7
94 schema:sdDatePublished 2019-04-11T13:21
95 schema:sdLicense https://scigraph.springernature.com/explorer/license/
96 schema:sdPublisher Ne381625c78704b21ab97cdf284bcebb5
97 schema:url https://link.springer.com/10.1007%2Fs00018-018-2993-7
98 sgo:license sg:explorer/license/
99 sgo:sdDataset articles
100 rdf:type schema:ScholarlyArticle
101 N19874f210b5d40bdbce906c24b7e8179 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Oxadiazoles
103 rdf:type schema:DefinedTerm
104 N2d960355393a44f89696a9f9850a5a9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Humans
106 rdf:type schema:DefinedTerm
107 N51439aad386a44d9a7e481f9826c5abf schema:issueNumber 6
108 rdf:type schema:PublicationIssue
109 N52e0908876c24ddc9b897cb1243ce531 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Nicotinic Agonists
111 rdf:type schema:DefinedTerm
112 N5e827e0b65844340bb3adbfe3d47ac97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Protein Conformation
114 rdf:type schema:DefinedTerm
115 N6c6a97622431458296d6e407a2346d62 schema:volumeNumber 76
116 rdf:type schema:PublicationVolume
117 N7153aabf70d14e5f84aa48929dafb0f3 schema:name dimensions_id
118 schema:value pub.1111012116
119 rdf:type schema:PropertyValue
120 N71ba4444662e4ae09379992d4ae2953e schema:name pubmed_id
121 schema:value 30600358
122 rdf:type schema:PropertyValue
123 N79e3c20a261c4f0bababf61c79493685 schema:name nlm_unique_id
124 schema:value 9705402
125 rdf:type schema:PropertyValue
126 N7d8b6097434f4089b50aa1240ca0a125 schema:name readcube_id
127 schema:value 105aaee2904c9fdc9ed4f268a2b4d52e4c267b740bc7fc2509c2c2d6633b8954
128 rdf:type schema:PropertyValue
129 N89bd54daa3de4c9a84bb52359c4dd513 rdf:first sg:person.01277141022.80
130 rdf:rest Nf9b299e533ba4252ada2fb35b12eb865
131 N92c6e30f64404fa9a86940fe582a47ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Models, Molecular
133 rdf:type schema:DefinedTerm
134 N9d7329a0ed7f48bda48e78351da5963d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Acetylcholine
136 rdf:type schema:DefinedTerm
137 Na0005b18daa745e4ba7301f2bac08bca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Binding Sites
139 rdf:type schema:DefinedTerm
140 Na4faa6a1fae64a54ae7c5b7eb768140a rdf:first sg:person.012453140337.73
141 rdf:rest rdf:nil
142 Nafb1e1f88f1f42b2a4aab05ef7650e67 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Mutation
144 rdf:type schema:DefinedTerm
145 Nb40f164bfeca4a379681462169c169ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Neurons
147 rdf:type schema:DefinedTerm
148 Nc5b8a19fa73e47778d36a565cc5b71a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Protein Isoforms
150 rdf:type schema:DefinedTerm
151 Ncf0d06674b3740b6be5496f85fa8d361 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name HEK293 Cells
153 rdf:type schema:DefinedTerm
154 Nd7bfb9a8f402408983ece02534271d31 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Protein Subunits
156 rdf:type schema:DefinedTerm
157 Nd94b29461c5a42caa501b44ace367578 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Pyridines
159 rdf:type schema:DefinedTerm
160 Ne1507c8fd12e4a7f93b3f9407d3444af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Receptors, Nicotinic
162 rdf:type schema:DefinedTerm
163 Ne381625c78704b21ab97cdf284bcebb5 schema:name Springer Nature - SN SciGraph project
164 rdf:type schema:Organization
165 Neb0efeb7b198469e89934ef2fbe69e4b schema:name doi
166 schema:value 10.1007/s00018-018-2993-7
167 rdf:type schema:PropertyValue
168 Nee6013dbb2064a08aa142fbd457d73aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Drug Synergism
170 rdf:type schema:DefinedTerm
171 Nf6dde0011bc04b1484212bb543ffe410 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Action Potentials
173 rdf:type schema:DefinedTerm
174 Nf9b299e533ba4252ada2fb35b12eb865 rdf:first sg:person.01332247171.05
175 rdf:rest Na4faa6a1fae64a54ae7c5b7eb768140a
176 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
177 schema:name Biological Sciences
178 rdf:type schema:DefinedTerm
179 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
180 schema:name Biochemistry and Cell Biology
181 rdf:type schema:DefinedTerm
182 sg:grant.2557573 http://pending.schema.org/fundedItem sg:pub.10.1007/s00018-018-2993-7
183 rdf:type schema:MonetaryGrant
184 sg:journal.1295005 schema:issn 1420-682X
185 1420-9071
186 schema:name Cellular and Molecular Life Sciences
187 rdf:type schema:Periodical
188 sg:person.012453140337.73 schema:affiliation https://www.grid.ac/institutes/grid.66875.3a
189 schema:familyName Sine
190 schema:givenName Steven M.
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012453140337.73
192 rdf:type schema:Person
193 sg:person.01277141022.80 schema:affiliation https://www.grid.ac/institutes/grid.66875.3a
194 schema:familyName Mazzaferro
195 schema:givenName Simone
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277141022.80
197 rdf:type schema:Person
198 sg:person.01332247171.05 schema:affiliation https://www.grid.ac/institutes/grid.7628.b
199 schema:familyName Bermudez
200 schema:givenName Isabel
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332247171.05
202 rdf:type schema:Person
203 sg:pub.10.1007/978-1-4615-7858-1_11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007617656
204 https://doi.org/10.1007/978-1-4615-7858-1_11
205 rdf:type schema:CreativeWork
206 sg:pub.10.1007/s00213-016-4475-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005498689
207 https://doi.org/10.1007/s00213-016-4475-7
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/19756 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013295470
210 https://doi.org/10.1038/19756
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/374065a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016922165
213 https://doi.org/10.1038/374065a0
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/nature19785 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036145124
216 https://doi.org/10.1038/nature19785
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nrd2927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049839544
219 https://doi.org/10.1038/nrd2927
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nrn1298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028421274
222 https://doi.org/10.1038/nrn1298
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/s41586-018-0081-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103702825
225 https://doi.org/10.1038/s41586-018-0081-7
226 rdf:type schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1082666162 schema:CreativeWork
228 https://doi.org/10.1016/0896-6273(94)90424-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016705074
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.bcp.2011.05.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031079549
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.jmb.2004.12.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036771134
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/j.neuropharm.2008.07.041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043666130
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.pharmthera.2012.08.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007973268
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/s0006-3495(87)83298-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050989941
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/s0165-6147(97)90675-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024388735
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1073/pnas.0804372105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034495968
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1073/pnas.1301909110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012356886
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.90.18.8392 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035790995
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.90.20.9436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010448395
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1074/jbc.274.38.27145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022242550
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1074/jbc.m011549200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024443795
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1074/jbc.m111.262014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004137052
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1074/jbc.m113.498618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045269703
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1074/jbc.m114.555136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031514339
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1074/jbc.m114.568097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050779471
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1074/jbc.m115.684373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058213963
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1074/jbc.m116.751206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058215530
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1074/jbc.m116.764183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058215788
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1074/jbc.m116.771246 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085276787
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1074/jbc.r112.386664 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029615124
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1085/jgp.201611584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041251576
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1085/jgp.201711846 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100715462
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1085/jgp.96.2.395 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005170727
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1101/cshperspect.a012112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044197018
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1111/bph.12095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044664390
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1111/bph.12268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047294556
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1111/bph.13905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085968495
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1111/j.1471-4159.2006.03915.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031708835
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1111/j.1476-5381.2008.00104.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046674097
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1111/j.1476-5381.2012.01989.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020193118
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1124/jpet.113.208389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017548461
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1124/jpet.115.223933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040568779
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1124/mol.105.015438 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035023675
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1124/mol.106.023044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043350094
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1124/mol.111.076059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036412441
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1124/mol.116.106880 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090613954
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1124/mol.54.6.1124 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083362538
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1124/mol.63.2.332 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044839487
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1152/physrev.00015.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033465396
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1523/jneurosci.0448-08.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044970751
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1523/jneurosci.1228-08.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036431366
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1523/jneurosci.18-12-04461.1998 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083273115
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1523/jneurosci.1859-09.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051026320
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1523/jneurosci.20-17-06431.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074691708
319 rdf:type schema:CreativeWork
320 https://doi.org/10.2174/156802611794863562 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069194065
321 rdf:type schema:CreativeWork
322 https://www.grid.ac/institutes/grid.66875.3a schema:alternateName Mayo Clinic
323 schema:name Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA
324 Department of Neurology, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA
325 Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, 55905, Rochester, MN, USA
326 rdf:type schema:Organization
327 https://www.grid.ac/institutes/grid.7628.b schema:alternateName Oxford Brookes University
328 schema:name School of Life Sciences, Oxford Brookes University, OX3 OBP, Oxford, UK
329 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...