microRNA-122 target sites in the hepatitis C virus RNA NS5B coding region and 3′ untranslated region: function in replication and ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-02

AUTHORS

Gesche K. Gerresheim, Nadia Dünnes, Anika Nieder-Röhrmann, Lyudmila A. Shalamova, Markus Fricke, Ivo Hofacker, Christian Höner zu Siederdissen, Manja Marz, Michael Niepmann

ABSTRACT

We have analyzed the binding of the liver-specific microRNA-122 (miR-122) to three conserved target sites of hepatitis C virus (HCV) RNA, two in the non-structural protein 5B (NS5B) coding region and one in the 3' untranslated region (3'UTR). miR-122 binding efficiency strongly depends on target site accessibility under conditions when the range of flanking sequences available for the formation of local RNA secondary structures changes. Our results indicate that the particular sequence feature that contributes most to the correlation between target site accessibility and binding strength varies between different target sites. This suggests that the dynamics of miRNA/Ago2 binding not only depends on the target site itself but also on flanking sequence context to a considerable extent, in particular in a small viral genome in which strong selection constraints act on coding sequence and overlapping cis-signals and model the accessibility of cis-signals. In full-length genomes, single and combination mutations in the miR-122 target sites reveal that site 5B.2 is positively involved in regulating overall genome replication efficiency, whereas mutation of site 5B.3 showed a weaker effect. Mutation of the 3'UTR site and double or triple mutants showed no significant overall effect on genome replication, whereas in a translation reporter RNA, the 3'UTR target site inhibits translation directed by the HCV 5'UTR. Thus, the miR-122 target sites in the 3'-region of the HCV genome are involved in a complex interplay in regulating different steps of the HCV replication cycle. More... »

PAGES

747-760

References to SciGraph publications

  • 2007-10. The role of site accessibility in microRNA target recognition in NATURE GENETICS
  • 2013. Hepatitis C Virus Proteins: From Structure to Function in HEPATITIS C VIRUS: FROM MOLECULAR VIROLOGY TO ANTIVIRAL THERAPY
  • 2013. Hepatitis C Virus RNA Translation in HEPATITIS C VIRUS: FROM MOLECULAR VIROLOGY TO ANTIVIRAL THERAPY
  • 2013. Hepatitis C Virus RNA Replication in HEPATITIS C VIRUS: FROM MOLECULAR VIROLOGY TO ANTIVIRAL THERAPY
  • 2013-03. A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets in NATURE METHODS
  • 2011-02. Gene silencing by microRNAs: contributions of translational repression and mRNA decay in NATURE REVIEWS GENETICS
  • 2007-10. How microRNAs choose their targets in NATURE GENETICS
  • 2011-12. ViennaRNA Package 2.0 in ALGORITHMS FOR MOLECULAR BIOLOGY
  • 2012-06. The mechanics of miRNA-mediated gene silencing: a look under the hood of miRISC in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2009-01-22. A three-dimensional view of the molecular machinery of RNA interference in NATURE
  • 2007-04. Potent effect of target structure on microRNA function in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2008-12-18. Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00018-016-2377-9

    DOI

    http://dx.doi.org/10.1007/s00018-016-2377-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049520746

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27677491


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "3' Untranslated Regions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "5' Untranslated Regions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Argonaute Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Viral", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Viral", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HeLa Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hepacivirus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hepatitis C", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "MicroRNAs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Conformation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Biosynthesis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Viral", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Viral Nonstructural Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Virus Replication", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Giessen", 
              "id": "https://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Friedrichstrasse 24, 35392, Giessen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gerresheim", 
            "givenName": "Gesche K.", 
            "id": "sg:person.010330451155.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010330451155.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Giessen", 
              "id": "https://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Friedrichstrasse 24, 35392, Giessen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "D\u00fcnnes", 
            "givenName": "Nadia", 
            "id": "sg:person.01315277131.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315277131.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Giessen", 
              "id": "https://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Friedrichstrasse 24, 35392, Giessen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nieder-R\u00f6hrmann", 
            "givenName": "Anika", 
            "id": "sg:person.013433472565.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013433472565.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Giessen", 
              "id": "https://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Friedrichstrasse 24, 35392, Giessen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shalamova", 
            "givenName": "Lyudmila A.", 
            "id": "sg:person.01131010433.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131010433.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Friedrich Schiller University Jena", 
              "id": "https://www.grid.ac/institutes/grid.9613.d", 
              "name": [
                "Faculty of Mathematics and Computer Science, Friedrich-Schiller-University, 07743, Jena, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fricke", 
            "givenName": "Markus", 
            "id": "sg:person.0726605620.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726605620.21"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.10420.37", 
              "name": [
                "Institute for Theoretical Chemistry, University of Vienna, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hofacker", 
            "givenName": "Ivo", 
            "id": "sg:person.01222322364.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222322364.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.10420.37", 
              "name": [
                "Institute for Theoretical Chemistry, University of Vienna, 1090, Vienna, Austria", 
                "Bioinformatics Group, Department of Computer Science, and Interdisciplinary Center for Bioinformatics, Universit\u00e4t Leipzig, 04107, Leipzig, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "H\u00f6ner zu Siederdissen", 
            "givenName": "Christian", 
            "id": "sg:person.010213207253.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010213207253.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fritz Lipmann Institute", 
              "id": "https://www.grid.ac/institutes/grid.418245.e", 
              "name": [
                "Faculty of Mathematics and Computer Science, Friedrich-Schiller-University, 07743, Jena, Germany", 
                "FLI Leibniz Institute for Age Research, 07743, Jena, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Marz", 
            "givenName": "Manja", 
            "id": "sg:person.0635607332.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635607332.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Giessen", 
              "id": "https://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Friedrichstrasse 24, 35392, Giessen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Niepmann", 
            "givenName": "Michael", 
            "id": "sg:person.01175010547.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175010547.19"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.cell.2013.03.043", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000605111"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virol.2010.11.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002721909"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1114016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003059448"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2015.02.025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003684444"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp250", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003953433"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.973808", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005465897"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng2135", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006264760", 
              "https://doi.org/10.1038/ng2135"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2341", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006584393", 
              "https://doi.org/10.1038/nmeth.2341"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bies.201300104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008001335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkq768", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008138588"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bst0361216", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010824122"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bst0361216", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010824122"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.00675-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011685846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1007-1191", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011848562", 
              "https://doi.org/10.1038/ng1007-1191"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb1226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012306772", 
              "https://doi.org/10.1038/nsmb1226"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb1226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012306772", 
              "https://doi.org/10.1038/nsmb1226"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkr426", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012613897"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virusres.2012.05.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016659256"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1416266112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017733510"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-27340-7_5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017872881", 
              "https://doi.org/10.1007/978-3-642-27340-7_5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1213515110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018646232"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-27340-7_6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020066810", 
              "https://doi.org/10.1007/978-3-642-27340-7_6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2004.12.035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020132624"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.25.21.9198-9208.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020519633"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.033282.112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021381269"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07755", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022586447", 
              "https://doi.org/10.1038/nature07755"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.2348111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022899212"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1113329", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023422868"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.01.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023682432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.768207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025827962"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.00417-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026056600"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0401799101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026962739"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.2296", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028707295", 
              "https://doi.org/10.1038/nsmb.2296"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07666", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029382170", 
              "https://doi.org/10.1038/nature07666"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt435", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030938936"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.02046-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031848342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1006172107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035363436"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/rna.18827", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037630749"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/emboj.2008.244", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037766368"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-27340-7_7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038849295", 
              "https://doi.org/10.1007/978-3-642-27340-7_7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkn188", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040192872"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1112263109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040267711"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2010.03.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041414025"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.049338.114", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041828921"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2008.05.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043611712"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.285.5424.110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043934394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1053/j.gastro.2003.09.023", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045391658"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.00513-12", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045517010"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg2936", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046015375", 
              "https://doi.org/10.1038/nrg2936"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1748-7188-6-26", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046184001", 
              "https://doi.org/10.1186/1748-7188-6-26"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0504877103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047375250"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0056272", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047676414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2014.08.028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047689598"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt075", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052697274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/vir.0.18997-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060399851"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1258040", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062470249"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/cc.11.2.18699", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072296535"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/cc.8.10.8349", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072299992"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/rna.1.2.1066", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072309928"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/rna.4.2.4640", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072310361"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083326846", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-02", 
        "datePublishedReg": "2017-02-01", 
        "description": "We have analyzed the binding of the liver-specific microRNA-122 (miR-122) to three conserved target sites of hepatitis C virus (HCV) RNA, two in the non-structural protein 5B (NS5B) coding region and one in the 3' untranslated region (3'UTR). miR-122 binding efficiency strongly depends on target site accessibility under conditions when the range of flanking sequences available for the formation of local RNA secondary structures changes. Our results indicate that the particular sequence feature that contributes most to the correlation between target site accessibility and binding strength varies between different target sites. This suggests that the dynamics of miRNA/Ago2 binding not only depends on the target site itself but also on flanking sequence context to a considerable extent, in particular in a small viral genome in which strong selection constraints act on coding sequence and overlapping cis-signals and model the accessibility of cis-signals. In full-length genomes, single and combination mutations in the miR-122 target sites reveal that site 5B.2 is positively involved in regulating overall genome replication efficiency, whereas mutation of site 5B.3 showed a weaker effect. Mutation of the 3'UTR site and double or triple mutants showed no significant overall effect on genome replication, whereas in a translation reporter RNA, the 3'UTR target site inhibits translation directed by the HCV 5'UTR. Thus, the miR-122 target sites in the 3'-region of the HCV genome are involved in a complex interplay in regulating different steps of the HCV replication cycle.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00018-016-2377-9", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1295005", 
            "issn": [
              "1420-682X", 
              "1420-9071"
            ], 
            "name": "Cellular and Molecular Life Sciences", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "74"
          }
        ], 
        "name": "microRNA-122 target sites in the hepatitis C virus RNA NS5B coding region and 3\u2032 untranslated region: function in replication and influence of RNA secondary structure", 
        "pagination": "747-760", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "ebe7816c7ca8a04f6157e2b9bee5fe3411d4992b2881917d72d2aa7133579fe2"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "27677491"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9705402"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00018-016-2377-9"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1049520746"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00018-016-2377-9", 
          "https://app.dimensions.ai/details/publication/pub.1049520746"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:39", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70043_00000002.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs00018-016-2377-9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00018-016-2377-9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00018-016-2377-9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00018-016-2377-9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00018-016-2377-9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    388 TRIPLES      21 PREDICATES      104 URIs      37 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00018-016-2377-9 schema:about N01a60f0cb5f64c82ad9254dacb799e72
    2 N11667d6b965c4f888286ced06d5cd0c3
    3 N151d8a3cabf64db59163405943bd4c0b
    4 N1879ecd9fc16415c8389df7d527e5f51
    5 N196bcd90a00848998b04eee3a3a822a7
    6 N1caa0098d86b477384bbae18c75c6180
    7 N311d00d02843484c858125af0ef406ee
    8 N3e03c5f0b31044e582c9051269551ac2
    9 N432bb141e5d241078e64fda6642f3abc
    10 N940f3f6065b84ab7a8a050594ebec6e4
    11 Na50a889e952146fbbc2bcc554ed8abb7
    12 Nb0878131b8154022b09b448d2fd87076
    13 Nb53e2dba5deb44449b5ab86117371559
    14 Nb86398ae017045da9b6c4a0b95590bfe
    15 Nbac0e538ff7b4571952b84da0347ffc6
    16 Nbaeb014d475e49688923876b36b2ad6e
    17 anzsrc-for:06
    18 anzsrc-for:0604
    19 schema:author N334790c9425e4092b70f944451ba9223
    20 schema:citation sg:pub.10.1007/978-3-642-27340-7_5
    21 sg:pub.10.1007/978-3-642-27340-7_6
    22 sg:pub.10.1007/978-3-642-27340-7_7
    23 sg:pub.10.1038/nature07666
    24 sg:pub.10.1038/nature07755
    25 sg:pub.10.1038/ng1007-1191
    26 sg:pub.10.1038/ng2135
    27 sg:pub.10.1038/nmeth.2341
    28 sg:pub.10.1038/nrg2936
    29 sg:pub.10.1038/nsmb.2296
    30 sg:pub.10.1038/nsmb1226
    31 sg:pub.10.1186/1748-7188-6-26
    32 https://app.dimensions.ai/details/publication/pub.1083326846
    33 https://doi.org/10.1002/bies.201300104
    34 https://doi.org/10.1016/j.cell.2004.12.035
    35 https://doi.org/10.1016/j.cell.2009.01.002
    36 https://doi.org/10.1016/j.cell.2010.03.009
    37 https://doi.org/10.1016/j.cell.2013.03.043
    38 https://doi.org/10.1016/j.cell.2015.02.025
    39 https://doi.org/10.1016/j.molcel.2008.05.001
    40 https://doi.org/10.1016/j.molcel.2014.08.028
    41 https://doi.org/10.1016/j.virol.2010.11.015
    42 https://doi.org/10.1016/j.virusres.2012.05.022
    43 https://doi.org/10.1038/emboj.2008.244
    44 https://doi.org/10.1042/bst0361216
    45 https://doi.org/10.1053/j.gastro.2003.09.023
    46 https://doi.org/10.1073/pnas.0401799101
    47 https://doi.org/10.1073/pnas.0504877103
    48 https://doi.org/10.1073/pnas.1006172107
    49 https://doi.org/10.1073/pnas.1112263109
    50 https://doi.org/10.1073/pnas.1213515110
    51 https://doi.org/10.1073/pnas.1416266112
    52 https://doi.org/10.1093/bioinformatics/btp250
    53 https://doi.org/10.1093/nar/gkn188
    54 https://doi.org/10.1093/nar/gkq768
    55 https://doi.org/10.1093/nar/gkr426
    56 https://doi.org/10.1093/nar/gkt075
    57 https://doi.org/10.1093/nar/gkt435
    58 https://doi.org/10.1099/vir.0.18997-0
    59 https://doi.org/10.1126/science.1113329
    60 https://doi.org/10.1126/science.1114016
    61 https://doi.org/10.1126/science.1258040
    62 https://doi.org/10.1126/science.285.5424.110
    63 https://doi.org/10.1128/jvi.00417-10
    64 https://doi.org/10.1128/jvi.00513-12
    65 https://doi.org/10.1128/jvi.00675-06
    66 https://doi.org/10.1128/jvi.02046-10
    67 https://doi.org/10.1128/mcb.25.21.9198-9208.2005
    68 https://doi.org/10.1261/rna.033282.112
    69 https://doi.org/10.1261/rna.049338.114
    70 https://doi.org/10.1261/rna.2348111
    71 https://doi.org/10.1261/rna.768207
    72 https://doi.org/10.1261/rna.973808
    73 https://doi.org/10.1371/journal.pone.0056272
    74 https://doi.org/10.4161/cc.11.2.18699
    75 https://doi.org/10.4161/cc.8.10.8349
    76 https://doi.org/10.4161/rna.1.2.1066
    77 https://doi.org/10.4161/rna.18827
    78 https://doi.org/10.4161/rna.4.2.4640
    79 schema:datePublished 2017-02
    80 schema:datePublishedReg 2017-02-01
    81 schema:description We have analyzed the binding of the liver-specific microRNA-122 (miR-122) to three conserved target sites of hepatitis C virus (HCV) RNA, two in the non-structural protein 5B (NS5B) coding region and one in the 3' untranslated region (3'UTR). miR-122 binding efficiency strongly depends on target site accessibility under conditions when the range of flanking sequences available for the formation of local RNA secondary structures changes. Our results indicate that the particular sequence feature that contributes most to the correlation between target site accessibility and binding strength varies between different target sites. This suggests that the dynamics of miRNA/Ago2 binding not only depends on the target site itself but also on flanking sequence context to a considerable extent, in particular in a small viral genome in which strong selection constraints act on coding sequence and overlapping cis-signals and model the accessibility of cis-signals. In full-length genomes, single and combination mutations in the miR-122 target sites reveal that site 5B.2 is positively involved in regulating overall genome replication efficiency, whereas mutation of site 5B.3 showed a weaker effect. Mutation of the 3'UTR site and double or triple mutants showed no significant overall effect on genome replication, whereas in a translation reporter RNA, the 3'UTR target site inhibits translation directed by the HCV 5'UTR. Thus, the miR-122 target sites in the 3'-region of the HCV genome are involved in a complex interplay in regulating different steps of the HCV replication cycle.
    82 schema:genre research_article
    83 schema:inLanguage en
    84 schema:isAccessibleForFree false
    85 schema:isPartOf Na08ffed148e8472895f4e81727518ba6
    86 Nded75fae5c064adcbbacf88545ac46c1
    87 sg:journal.1295005
    88 schema:name microRNA-122 target sites in the hepatitis C virus RNA NS5B coding region and 3′ untranslated region: function in replication and influence of RNA secondary structure
    89 schema:pagination 747-760
    90 schema:productId N590d5870a0a74ee89caa8d3ee22358e4
    91 N6adc61ea275d40d288232452951949f3
    92 N82687fd225fc4b8f9425b11fa0d91e6e
    93 Nd6e8ddca839f44a892fd12eb6ca338b6
    94 Ndfd8f388e66949809a45f6f04c071bdc
    95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049520746
    96 https://doi.org/10.1007/s00018-016-2377-9
    97 schema:sdDatePublished 2019-04-11T12:39
    98 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    99 schema:sdPublisher N55bbfcfe88b44d3d8aa8e192bd1150fa
    100 schema:url https://link.springer.com/10.1007%2Fs00018-016-2377-9
    101 sgo:license sg:explorer/license/
    102 sgo:sdDataset articles
    103 rdf:type schema:ScholarlyArticle
    104 N01a60f0cb5f64c82ad9254dacb799e72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Viral Nonstructural Proteins
    106 rdf:type schema:DefinedTerm
    107 N11667d6b965c4f888286ced06d5cd0c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Hepacivirus
    109 rdf:type schema:DefinedTerm
    110 N151d8a3cabf64db59163405943bd4c0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name HeLa Cells
    112 rdf:type schema:DefinedTerm
    113 N1879ecd9fc16415c8389df7d527e5f51 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Argonaute Proteins
    115 rdf:type schema:DefinedTerm
    116 N196bcd90a00848998b04eee3a3a822a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name RNA, Viral
    118 rdf:type schema:DefinedTerm
    119 N1caa0098d86b477384bbae18c75c6180 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name 5' Untranslated Regions
    121 rdf:type schema:DefinedTerm
    122 N311d00d02843484c858125af0ef406ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Genome, Viral
    124 rdf:type schema:DefinedTerm
    125 N334790c9425e4092b70f944451ba9223 rdf:first sg:person.010330451155.26
    126 rdf:rest N391dfd39568f4a3db32a1669d9750d6a
    127 N340576af159541bd831fc445786ba77c rdf:first sg:person.01222322364.52
    128 rdf:rest N94b7c52e31a04bc3beda17b0e64d8886
    129 N391dfd39568f4a3db32a1669d9750d6a rdf:first sg:person.01315277131.94
    130 rdf:rest N9fa7ae0fdc664185962dea6ba858549b
    131 N3e03c5f0b31044e582c9051269551ac2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Virus Replication
    133 rdf:type schema:DefinedTerm
    134 N432bb141e5d241078e64fda6642f3abc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Hepatitis C
    136 rdf:type schema:DefinedTerm
    137 N55bbfcfe88b44d3d8aa8e192bd1150fa schema:name Springer Nature - SN SciGraph project
    138 rdf:type schema:Organization
    139 N590d5870a0a74ee89caa8d3ee22358e4 schema:name readcube_id
    140 schema:value ebe7816c7ca8a04f6157e2b9bee5fe3411d4992b2881917d72d2aa7133579fe2
    141 rdf:type schema:PropertyValue
    142 N6adc61ea275d40d288232452951949f3 schema:name dimensions_id
    143 schema:value pub.1049520746
    144 rdf:type schema:PropertyValue
    145 N82687fd225fc4b8f9425b11fa0d91e6e schema:name doi
    146 schema:value 10.1007/s00018-016-2377-9
    147 rdf:type schema:PropertyValue
    148 N940f3f6065b84ab7a8a050594ebec6e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name MicroRNAs
    150 rdf:type schema:DefinedTerm
    151 N94b7c52e31a04bc3beda17b0e64d8886 rdf:first sg:person.010213207253.25
    152 rdf:rest N9623f87c30a44238935f3d18fe4f60ff
    153 N9623f87c30a44238935f3d18fe4f60ff rdf:first sg:person.0635607332.82
    154 rdf:rest Ne6aa3bdcb1884c5fb69baee093ac831d
    155 N9fa7ae0fdc664185962dea6ba858549b rdf:first sg:person.013433472565.40
    156 rdf:rest Nedf482cf4e8544cda84a63eb82a96568
    157 Na08ffed148e8472895f4e81727518ba6 schema:issueNumber 4
    158 rdf:type schema:PublicationIssue
    159 Na50a889e952146fbbc2bcc554ed8abb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name 3' Untranslated Regions
    161 rdf:type schema:DefinedTerm
    162 Nb0878131b8154022b09b448d2fd87076 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Gene Expression Regulation, Viral
    164 rdf:type schema:DefinedTerm
    165 Nb53e2dba5deb44449b5ab86117371559 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    166 schema:name Protein Biosynthesis
    167 rdf:type schema:DefinedTerm
    168 Nb86398ae017045da9b6c4a0b95590bfe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Nucleic Acid Conformation
    170 rdf:type schema:DefinedTerm
    171 Nbac0e538ff7b4571952b84da0347ffc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Humans
    173 rdf:type schema:DefinedTerm
    174 Nbaeb014d475e49688923876b36b2ad6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Base Sequence
    176 rdf:type schema:DefinedTerm
    177 Nd5a1300c79434d54ba9eb4f0934a5924 rdf:first sg:person.0726605620.21
    178 rdf:rest N340576af159541bd831fc445786ba77c
    179 Nd6e8ddca839f44a892fd12eb6ca338b6 schema:name pubmed_id
    180 schema:value 27677491
    181 rdf:type schema:PropertyValue
    182 Nded75fae5c064adcbbacf88545ac46c1 schema:volumeNumber 74
    183 rdf:type schema:PublicationVolume
    184 Ndfd8f388e66949809a45f6f04c071bdc schema:name nlm_unique_id
    185 schema:value 9705402
    186 rdf:type schema:PropertyValue
    187 Ne6aa3bdcb1884c5fb69baee093ac831d rdf:first sg:person.01175010547.19
    188 rdf:rest rdf:nil
    189 Nedf482cf4e8544cda84a63eb82a96568 rdf:first sg:person.01131010433.34
    190 rdf:rest Nd5a1300c79434d54ba9eb4f0934a5924
    191 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    192 schema:name Biological Sciences
    193 rdf:type schema:DefinedTerm
    194 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    195 schema:name Genetics
    196 rdf:type schema:DefinedTerm
    197 sg:journal.1295005 schema:issn 1420-682X
    198 1420-9071
    199 schema:name Cellular and Molecular Life Sciences
    200 rdf:type schema:Periodical
    201 sg:person.010213207253.25 schema:affiliation https://www.grid.ac/institutes/grid.10420.37
    202 schema:familyName Höner zu Siederdissen
    203 schema:givenName Christian
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010213207253.25
    205 rdf:type schema:Person
    206 sg:person.010330451155.26 schema:affiliation https://www.grid.ac/institutes/grid.8664.c
    207 schema:familyName Gerresheim
    208 schema:givenName Gesche K.
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010330451155.26
    210 rdf:type schema:Person
    211 sg:person.01131010433.34 schema:affiliation https://www.grid.ac/institutes/grid.8664.c
    212 schema:familyName Shalamova
    213 schema:givenName Lyudmila A.
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131010433.34
    215 rdf:type schema:Person
    216 sg:person.01175010547.19 schema:affiliation https://www.grid.ac/institutes/grid.8664.c
    217 schema:familyName Niepmann
    218 schema:givenName Michael
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175010547.19
    220 rdf:type schema:Person
    221 sg:person.01222322364.52 schema:affiliation https://www.grid.ac/institutes/grid.10420.37
    222 schema:familyName Hofacker
    223 schema:givenName Ivo
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222322364.52
    225 rdf:type schema:Person
    226 sg:person.01315277131.94 schema:affiliation https://www.grid.ac/institutes/grid.8664.c
    227 schema:familyName Dünnes
    228 schema:givenName Nadia
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315277131.94
    230 rdf:type schema:Person
    231 sg:person.013433472565.40 schema:affiliation https://www.grid.ac/institutes/grid.8664.c
    232 schema:familyName Nieder-Röhrmann
    233 schema:givenName Anika
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013433472565.40
    235 rdf:type schema:Person
    236 sg:person.0635607332.82 schema:affiliation https://www.grid.ac/institutes/grid.418245.e
    237 schema:familyName Marz
    238 schema:givenName Manja
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635607332.82
    240 rdf:type schema:Person
    241 sg:person.0726605620.21 schema:affiliation https://www.grid.ac/institutes/grid.9613.d
    242 schema:familyName Fricke
    243 schema:givenName Markus
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726605620.21
    245 rdf:type schema:Person
    246 sg:pub.10.1007/978-3-642-27340-7_5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017872881
    247 https://doi.org/10.1007/978-3-642-27340-7_5
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1007/978-3-642-27340-7_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020066810
    250 https://doi.org/10.1007/978-3-642-27340-7_6
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1007/978-3-642-27340-7_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038849295
    253 https://doi.org/10.1007/978-3-642-27340-7_7
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/nature07666 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029382170
    256 https://doi.org/10.1038/nature07666
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/nature07755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022586447
    259 https://doi.org/10.1038/nature07755
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/ng1007-1191 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011848562
    262 https://doi.org/10.1038/ng1007-1191
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/ng2135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006264760
    265 https://doi.org/10.1038/ng2135
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nmeth.2341 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006584393
    268 https://doi.org/10.1038/nmeth.2341
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/nrg2936 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046015375
    271 https://doi.org/10.1038/nrg2936
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/nsmb.2296 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028707295
    274 https://doi.org/10.1038/nsmb.2296
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/nsmb1226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012306772
    277 https://doi.org/10.1038/nsmb1226
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1186/1748-7188-6-26 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046184001
    280 https://doi.org/10.1186/1748-7188-6-26
    281 rdf:type schema:CreativeWork
    282 https://app.dimensions.ai/details/publication/pub.1083326846 schema:CreativeWork
    283 https://doi.org/10.1002/bies.201300104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008001335
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1016/j.cell.2004.12.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020132624
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1016/j.cell.2009.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023682432
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1016/j.cell.2010.03.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041414025
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1016/j.cell.2013.03.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000605111
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1016/j.cell.2015.02.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003684444
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1016/j.molcel.2008.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043611712
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1016/j.molcel.2014.08.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047689598
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1016/j.virol.2010.11.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002721909
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1016/j.virusres.2012.05.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016659256
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1038/emboj.2008.244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037766368
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1042/bst0361216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010824122
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.1053/j.gastro.2003.09.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045391658
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.1073/pnas.0401799101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026962739
    310 rdf:type schema:CreativeWork
    311 https://doi.org/10.1073/pnas.0504877103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047375250
    312 rdf:type schema:CreativeWork
    313 https://doi.org/10.1073/pnas.1006172107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035363436
    314 rdf:type schema:CreativeWork
    315 https://doi.org/10.1073/pnas.1112263109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040267711
    316 rdf:type schema:CreativeWork
    317 https://doi.org/10.1073/pnas.1213515110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018646232
    318 rdf:type schema:CreativeWork
    319 https://doi.org/10.1073/pnas.1416266112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017733510
    320 rdf:type schema:CreativeWork
    321 https://doi.org/10.1093/bioinformatics/btp250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003953433
    322 rdf:type schema:CreativeWork
    323 https://doi.org/10.1093/nar/gkn188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040192872
    324 rdf:type schema:CreativeWork
    325 https://doi.org/10.1093/nar/gkq768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008138588
    326 rdf:type schema:CreativeWork
    327 https://doi.org/10.1093/nar/gkr426 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012613897
    328 rdf:type schema:CreativeWork
    329 https://doi.org/10.1093/nar/gkt075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052697274
    330 rdf:type schema:CreativeWork
    331 https://doi.org/10.1093/nar/gkt435 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030938936
    332 rdf:type schema:CreativeWork
    333 https://doi.org/10.1099/vir.0.18997-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060399851
    334 rdf:type schema:CreativeWork
    335 https://doi.org/10.1126/science.1113329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023422868
    336 rdf:type schema:CreativeWork
    337 https://doi.org/10.1126/science.1114016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003059448
    338 rdf:type schema:CreativeWork
    339 https://doi.org/10.1126/science.1258040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062470249
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1126/science.285.5424.110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043934394
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1128/jvi.00417-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026056600
    344 rdf:type schema:CreativeWork
    345 https://doi.org/10.1128/jvi.00513-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045517010
    346 rdf:type schema:CreativeWork
    347 https://doi.org/10.1128/jvi.00675-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011685846
    348 rdf:type schema:CreativeWork
    349 https://doi.org/10.1128/jvi.02046-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031848342
    350 rdf:type schema:CreativeWork
    351 https://doi.org/10.1128/mcb.25.21.9198-9208.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020519633
    352 rdf:type schema:CreativeWork
    353 https://doi.org/10.1261/rna.033282.112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021381269
    354 rdf:type schema:CreativeWork
    355 https://doi.org/10.1261/rna.049338.114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041828921
    356 rdf:type schema:CreativeWork
    357 https://doi.org/10.1261/rna.2348111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022899212
    358 rdf:type schema:CreativeWork
    359 https://doi.org/10.1261/rna.768207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025827962
    360 rdf:type schema:CreativeWork
    361 https://doi.org/10.1261/rna.973808 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005465897
    362 rdf:type schema:CreativeWork
    363 https://doi.org/10.1371/journal.pone.0056272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047676414
    364 rdf:type schema:CreativeWork
    365 https://doi.org/10.4161/cc.11.2.18699 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072296535
    366 rdf:type schema:CreativeWork
    367 https://doi.org/10.4161/cc.8.10.8349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072299992
    368 rdf:type schema:CreativeWork
    369 https://doi.org/10.4161/rna.1.2.1066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072309928
    370 rdf:type schema:CreativeWork
    371 https://doi.org/10.4161/rna.18827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037630749
    372 rdf:type schema:CreativeWork
    373 https://doi.org/10.4161/rna.4.2.4640 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072310361
    374 rdf:type schema:CreativeWork
    375 https://www.grid.ac/institutes/grid.10420.37 schema:alternateName University of Vienna
    376 schema:name Bioinformatics Group, Department of Computer Science, and Interdisciplinary Center for Bioinformatics, Universität Leipzig, 04107, Leipzig, Germany
    377 Institute for Theoretical Chemistry, University of Vienna, 1090, Vienna, Austria
    378 rdf:type schema:Organization
    379 https://www.grid.ac/institutes/grid.418245.e schema:alternateName Fritz Lipmann Institute
    380 schema:name FLI Leibniz Institute for Age Research, 07743, Jena, Germany
    381 Faculty of Mathematics and Computer Science, Friedrich-Schiller-University, 07743, Jena, Germany
    382 rdf:type schema:Organization
    383 https://www.grid.ac/institutes/grid.8664.c schema:alternateName University of Giessen
    384 schema:name Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Friedrichstrasse 24, 35392, Giessen, Germany
    385 rdf:type schema:Organization
    386 https://www.grid.ac/institutes/grid.9613.d schema:alternateName Friedrich Schiller University Jena
    387 schema:name Faculty of Mathematics and Computer Science, Friedrich-Schiller-University, 07743, Jena, Germany
    388 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...