Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-05

AUTHORS

Pablo Bosch, Diego O. Forcato, Fabrisio E. Alustiza, Ana P. Alessio, Alejandro E. Fili, María F. Olmos Nicotra, Ana C. Liaudat, Nancy Rodríguez, Thirumala R. Talluri, Wilfried A. Kues

ABSTRACT

Transgenic farm animals are attractive alternative mammalian models to rodents for the study of developmental, genetic, reproductive and disease-related biological questions, as well for the production of recombinant proteins, or the assessment of xenotransplants for human patients. Until recently, the ability to generate transgenic farm animals relied on methods of passive transgenesis. In recent years, significant improvements have been made to introduce and apply active techniques of transgenesis and genetic engineering in these species. These new approaches dramatically enhance the ease and speed with which livestock species can be genetically modified, and allow to performing precise genetic modifications. This paper provides a synopsis of enzyme-mediated genetic engineering in livestock species covering the early attempts employing naturally occurring DNA-modifying proteins to recent approaches working with tailored enzymatic systems. More... »

PAGES

1907-1929

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00018-015-1842-1

    DOI

    http://dx.doi.org/10.1007/s00018-015-1842-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032477412

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25636347


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