Ontology type: schema:ScholarlyArticle
2012-12-27
AUTHORSStylianos Michalakis, Karin Schäferhoff, Isabella Spiwoks-Becker, Nawal Zabouri, Susanne Koch, Fred Koch, Michael Bonin, Martin Biel, Silke Haverkamp
ABSTRACTIn the mammalian retina, light signals generated in photoreceptors are passed to bipolar and horizontal cells via synaptic contacts. In various pathological conditions, these second-order neurons extend neurites into the outer nuclear layer (ONL). However, the molecular events associated with this neurite outgrowth are not known. Here, we characterized the morphological synaptic changes in the CNGA3/CNGB1 double-knockout (A3B1) mouse, a model of retinitis pigmentosa. In these mice, horizontal cells looked normal until postnatal day (p) 11, but started growing neurites into the ONL 1 day later. At p28, the number of sprouting processes decreased, but the remaining sprouts developed synapse-like contacts at rod cell bodies, with an ultrastructural appearance reminiscent of ribbon synapses. Hence, neurite outgrowth and ectopic synaptogenesis in the A3B1 retina were precisely timed events starting at p12 and p28, respectively. We therefore performed microarray analysis of retinal gene expression in A3B1 and wild-type mice at those ages to evaluate the genomic response underlying these two events. This analysis identified 163 differentially regulated genes in the A3B1 retina related to neurite outgrowth or plasticity of synapses. The global changes in gene expression in the A3B1 retina were consistent with activation of signaling pathways related to Tp53, Smad, and Stat3. Moreover, key molecules of these signaling pathways could be localized at or in close proximity to outgrowing neurites. We therefore propose that Tp53, Smad, and Stat3 signaling pathways contribute to the synaptic plasticity in the A3B1 retina. More... »
PAGES1831-1847
http://scigraph.springernature.com/pub.10.1007/s00018-012-1230-z
DOIhttp://dx.doi.org/10.1007/s00018-012-1230-z
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1028413005
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/23269435
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Neurosciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cyclic Nucleotide-Gated Cation Channels",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Disease Models, Animal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Regulatory Networks",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Inbred C57BL",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Knockout",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nerve Tissue Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neurites",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neuronal Plasticity",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Retina",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Retinal Bipolar Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Retinal Horizontal Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Retinitis Pigmentosa",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "STAT3 Transcription Factor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Signal Transduction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Smad Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Synapses",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tumor Suppressor Protein p53",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany",
"id": "http://www.grid.ac/institutes/grid.5252.0",
"name": [
"Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany"
],
"type": "Organization"
},
"familyName": "Michalakis",
"givenName": "Stylianos",
"id": "sg:person.01347514155.59",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347514155.59"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Medical Genetics and Applied Genomics, University Hospital T\u00fcbingen, Calwerstr. 7, 72076, Tuebingen, Germany",
"id": "http://www.grid.ac/institutes/grid.411544.1",
"name": [
"Institute of Medical Genetics and Applied Genomics, University Hospital T\u00fcbingen, Calwerstr. 7, 72076, Tuebingen, Germany"
],
"type": "Organization"
},
"familyName": "Sch\u00e4ferhoff",
"givenName": "Karin",
"id": "sg:person.0726761334.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726761334.48"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Microscopic Anatomy and Neurobiology, University Medicine Mainz, Mainz, Germany",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Institute for Microscopic Anatomy and Neurobiology, University Medicine Mainz, Mainz, Germany"
],
"type": "Organization"
},
"familyName": "Spiwoks-Becker",
"givenName": "Isabella",
"id": "sg:person.01002340426.69",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002340426.69"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Neuroanatomy, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60528, Frankfurt, Germany",
"id": "http://www.grid.ac/institutes/grid.419505.c",
"name": [
"Neuroanatomy, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60528, Frankfurt, Germany"
],
"type": "Organization"
},
"familyName": "Zabouri",
"givenName": "Nawal",
"id": "sg:person.0600400721.72",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600400721.72"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany",
"id": "http://www.grid.ac/institutes/grid.5252.0",
"name": [
"Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany"
],
"type": "Organization"
},
"familyName": "Koch",
"givenName": "Susanne",
"id": "sg:person.0615334613.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615334613.44"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany",
"id": "http://www.grid.ac/institutes/grid.5252.0",
"name": [
"Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany"
],
"type": "Organization"
},
"familyName": "Koch",
"givenName": "Fred",
"id": "sg:person.01200001335.80",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200001335.80"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Medical Genetics and Applied Genomics, University Hospital T\u00fcbingen, Calwerstr. 7, 72076, Tuebingen, Germany",
"id": "http://www.grid.ac/institutes/grid.411544.1",
"name": [
"Institute of Medical Genetics and Applied Genomics, University Hospital T\u00fcbingen, Calwerstr. 7, 72076, Tuebingen, Germany"
],
"type": "Organization"
},
"familyName": "Bonin",
"givenName": "Michael",
"id": "sg:person.01322100653.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322100653.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany",
"id": "http://www.grid.ac/institutes/grid.5252.0",
"name": [
"Center for Integrated Protein Science Munich CiPSM and Department of Pharmacy \u2013 Center for Drug Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Butenandtstr 7, 81377, Munich, Germany"
],
"type": "Organization"
},
"familyName": "Biel",
"givenName": "Martin",
"id": "sg:person.01174276525.93",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174276525.93"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Neuroanatomy, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60528, Frankfurt, Germany",
"id": "http://www.grid.ac/institutes/grid.419505.c",
"name": [
"Neuroanatomy, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60528, Frankfurt, Germany"
],
"type": "Organization"
},
"familyName": "Haverkamp",
"givenName": "Silke",
"id": "sg:person.0675537636.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675537636.29"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nn1320",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043561582",
"https://doi.org/10.1038/nn1320"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nn.2231",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048266431",
"https://doi.org/10.1038/nn.2231"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00406-011-0242-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025318067",
"https://doi.org/10.1007/s00406-011-0242-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrn1497",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012560945",
"https://doi.org/10.1038/nrn1497"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00441-011-1292-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012304678",
"https://doi.org/10.1007/s00441-011-1292-5"
],
"type": "CreativeWork"
}
],
"datePublished": "2012-12-27",
"datePublishedReg": "2012-12-27",
"description": "In the mammalian retina, light signals generated in photoreceptors are passed to bipolar and horizontal cells via synaptic contacts. In various pathological conditions, these second-order neurons extend neurites into the outer nuclear layer (ONL). However, the molecular events associated with this neurite outgrowth are not known. Here, we characterized the morphological synaptic changes in the CNGA3/CNGB1 double-knockout (A3B1) mouse, a model of retinitis pigmentosa. In these mice, horizontal cells looked normal until postnatal day (p) 11, but started growing neurites into the ONL 1\u00a0day later. At p28, the number of sprouting processes decreased, but the remaining sprouts developed synapse-like contacts at rod cell bodies, with an ultrastructural appearance reminiscent of ribbon synapses. Hence, neurite outgrowth and ectopic synaptogenesis in the A3B1 retina were precisely timed events starting at p12 and p28, respectively. We therefore performed microarray analysis of retinal gene expression in A3B1 and wild-type mice at those ages to evaluate the genomic response underlying these two events. This analysis identified 163 differentially regulated genes in the A3B1 retina related to neurite outgrowth or plasticity of synapses. The global changes in gene expression in the A3B1 retina were consistent with activation of signaling pathways related to Tp53, Smad, and Stat3. Moreover, key molecules of these signaling pathways could be localized at or in close proximity to outgrowing neurites. We therefore propose that Tp53, Smad, and Stat3 signaling pathways contribute to the synaptic plasticity in the A3B1 retina.",
"genre": "article",
"id": "sg:pub.10.1007/s00018-012-1230-z",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1295005",
"issn": [
"1420-682X",
"1420-9071"
],
"name": "Cellular and Molecular Life Sciences",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "10",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "70"
}
],
"keywords": [
"neurite outgrowth",
"gene expression",
"ectopic synaptogenesis",
"plasticity of synapses",
"genomic responses",
"microarray analysis",
"molecular events",
"retinal gene expression",
"rod cell bodies",
"global change",
"key molecules",
"morphological synaptic changes",
"mammalian retina",
"double knockout mice",
"outer nuclear layer",
"ribbon synapses",
"pathway",
"Smad",
"outgrowth",
"STAT3",
"pathological conditions",
"synaptic plasticity",
"synapse-like contacts",
"light signals",
"sprouting process",
"plasticity",
"neurites",
"horizontal cells",
"expression",
"TP53",
"synaptogenesis",
"wild-type mice",
"cells",
"second-order neurons",
"postnatal day 11",
"cell bodies",
"genes",
"close proximity",
"mice",
"ultrastructural appearance",
"synapses",
"photoreceptors",
"synaptic contacts",
"P28",
"photoreceptor dysfunction",
"nuclear layer",
"retinitis pigmentosa",
"synaptic changes",
"day 11",
"retina",
"activation",
"sprouts",
"response",
"events",
"P12",
"molecules",
"characterization",
"neurons",
"changes",
"analysis",
"dysfunction",
"A3B1",
"proximity",
"pigmentosa",
"signals",
"age",
"contact",
"days",
"body",
"number",
"process",
"conditions",
"appearance",
"model",
"layer"
],
"name": "Characterization of neurite outgrowth and ectopic synaptogenesis in response to photoreceptor dysfunction",
"pagination": "1831-1847",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1028413005"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00018-012-1230-z"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"23269435"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00018-012-1230-z",
"https://app.dimensions.ai/details/publication/pub.1028413005"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T17:00",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_564.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00018-012-1230-z"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00018-012-1230-z'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00018-012-1230-z'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00018-012-1230-z'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00018-012-1230-z'
This table displays all metadata directly associated to this object as RDF triples.
309 TRIPLES
21 PREDICATES
127 URIs
112 LITERALS
27 BLANK NODES