Characterization of the self-palmitoylation activity of the transport protein particle component Bet3 View Full Text


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Article Info

DATE

2010-08

AUTHORS

Daniel Kümmel, Julia Walter, Martin Heck, Udo Heinemann, Michael Veit

ABSTRACT

Bet3, a transport protein particle component involved in vesicular trafficking, contains a hydrophobic tunnel occupied by a fatty acid linked to cysteine 68. We reported that Bet3 has a unique self-palmitoylating activity. Here we show that mutation of arginine 67 reduced self-palmitoylation of Bet3, but the effect was compensated by increasing the pH. Thus, arginine helps to deprotonate cysteine such that it could function as a nucleophile in the acylation reaction which is supported by the structural analysis of non-acylated Bet3. Using fluorescence spectroscopy we show that long-chain acyl-CoAs bind with micromolar affinity to Bet3, whereas shorter-chain acyl-CoAs do not interact. Mutants with a deleted acylation site or a blocked tunnel bind to Pal-CoA, only the latter with slightly reduced affinity. Bet3 contains three binding sites for Pal-CoA, but their number was reduced to two in the mutant with an obstructed tunnel, indicating that Bet3 contains binding sites on its surface. More... »

PAGES

2653-2664

References to SciGraph publications

  • 2007-01. Palmitoylation: policing protein stability and traffic in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2005-01. Crystal structure of bet3 reveals a novel mechanism for Golgi localization of tethering factor TRAPP in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2008-09. Discovery of protein-palmitoylating enzymes in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00018-010-0358-y

    DOI

    http://dx.doi.org/10.1007/s00018-010-0358-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1038520544

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20372964


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0358-y'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0358-y'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0358-y'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0358-y'


     

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