A llama-derived gelsolin single-domain antibody blocks gelsolin–G-actin interaction View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-05

AUTHORS

Anske Van den Abbeele, Sarah De Clercq, Ariane De Ganck, Veerle De Corte, Berlinda Van Loo, Sameh Hamdy Soror, Vasundara Srinivasan, Jan Steyaert, Joël Vandekerckhove, Jan Gettemans

ABSTRACT

RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated. More... »

PAGES

1519-1535

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00018-010-0266-1

DOI

http://dx.doi.org/10.1007/s00018-010-0266-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036584248

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20140750


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Camelids, New World", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallography, X-Ray", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epitopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gelsolin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitochondria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Single-Chain Antibodies", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Van den Abbeele", 
        "givenName": "Anske", 
        "id": "sg:person.01323027521.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323027521.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "De Clercq", 
        "givenName": "Sarah", 
        "id": "sg:person.0601624354.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601624354.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "De Ganck", 
        "givenName": "Ariane", 
        "id": "sg:person.0616250601.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616250601.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "De Corte", 
        "givenName": "Veerle", 
        "id": "sg:person.0776116714.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776116714.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Van Loo", 
        "givenName": "Berlinda", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Structural Biology, Free University of Brussels, Pleinlaan 2, 1050, Brussels, Belgium", 
            "Department of Molecular and Cellular Interactions, VIB, 1050, Brussels, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Soror", 
        "givenName": "Sameh Hamdy", 
        "id": "sg:person.01007752627.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007752627.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Structural Biology, Free University of Brussels, Pleinlaan 2, 1050, Brussels, Belgium", 
            "Department of Molecular and Cellular Interactions, VIB, 1050, Brussels, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Srinivasan", 
        "givenName": "Vasundara", 
        "id": "sg:person.01030403661.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030403661.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Structural Biology, Free University of Brussels, Pleinlaan 2, 1050, Brussels, Belgium", 
            "Department of Molecular and Cellular Interactions, VIB, 1050, Brussels, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Steyaert", 
        "givenName": "Jan", 
        "id": "sg:person.01155503100.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155503100.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vandekerckhove", 
        "givenName": "Jo\u00ebl", 
        "id": "sg:person.01036516654.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036516654.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Department of Medical Protein Research, VIB, 9000, Ghent, Belgium", 
            "Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gettemans", 
        "givenName": "Jan", 
        "id": "sg:person.01273101735.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273101735.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/emboj/17.20.5923", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002435548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0505379103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005266742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0812374106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005513276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1993.tb18403.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006838740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1994.tb20015.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008424189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0854.2005.00326.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009708797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0854.2005.00326.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009708797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444901014007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009737008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.76.9.4350", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011704795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth953", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012346597", 
          "https://doi.org/10.1038/nmeth953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth953", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012346597", 
          "https://doi.org/10.1038/nmeth953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2008.11.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014961753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(97)01062-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015552570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)01131-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016067722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)01131-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016067722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1359/jbmr.040128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016492035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2008.02.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017983226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/363446a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018109154", 
          "https://doi.org/10.1038/363446a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0709-671a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018408477", 
          "https://doi.org/10.1038/nbt0709-671a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444904019158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020281098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetimm.2008.10.299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020528313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(91)80206-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020925496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600872", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021409380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600872", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021409380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna5160904", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021691638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m602567200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025311211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444994003112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025573949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1993.1351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026297927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/17.5.1362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026367021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200101113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026509682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.104.3.491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027471936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444992007698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027900257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-004-4225-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029653312", 
          "https://doi.org/10.1007/s00018-004-4225-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029667713", 
          "https://doi.org/10.1038/nature08231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029667713", 
          "https://doi.org/10.1038/nature08231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdf680", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030008802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/circresaha.108.172882", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030358604"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/circresaha.108.172882", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030358604"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.116.5.1135", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030400004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb0996-733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032832494", 
          "https://doi.org/10.1038/nsb0996-733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.102.4.1439", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033081809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3240283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034328974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3240283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034328974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80527-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034781647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.06-6306fje", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034913466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(99)80049-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035578275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444903020924", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036336557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1543", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036769338", 
          "https://doi.org/10.1038/nbt.1543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2008.11.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036896722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.119.6.1451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036949715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.108.5.1717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038891627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041500941", 
          "https://doi.org/10.1038/ncb834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041500941", 
          "https://doi.org/10.1038/ncb834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.109.2.593", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042172228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.112.6.1151", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042903115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/9.6.531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043661194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600280", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044570454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/381272a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044595902", 
          "https://doi.org/10.1038/381272a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.canlet.2007.03.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045561525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.145.1.109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047412687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1521-4141(199911)29:11<3450::aid-immu3450>3.0.co;2-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047838040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1521-4141(199911)29:11<3450::aid-immu3450>3.0.co;2-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047838040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physrev.00021.2007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050366371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/circresaha.109.196329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050436736"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/circresaha.109.196329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050436736"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.45.10.2807-2812.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051826182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb0996-803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051868454", 
          "https://doi.org/10.1038/nsb0996-803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.165.1.97", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053069219"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/gut.2005.083691", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053322164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00156a028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055163023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1848726", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062509922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.286.5446.1939", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062567537"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075339595", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075455247", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077093941", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3233/jad-2009-1127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077934758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079500386", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081682349", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082095424", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-05", 
    "datePublishedReg": "2010-05-01", 
    "description": "RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00018-010-0266-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1295005", 
        "issn": [
          "1420-682X", 
          "1420-9071"
        ], 
        "name": "Cellular and Molecular Life Sciences", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "67"
      }
    ], 
    "name": "A llama-derived gelsolin single-domain antibody blocks gelsolin\u2013G-actin interaction", 
    "pagination": "1519-1535", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ae45d22f573c1c80c31ce3b93234d6905a9523d7161fc0c67179e84a9bad9a21"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20140750"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9705402"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00018-010-0266-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036584248"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00018-010-0266-1", 
      "https://app.dimensions.ai/details/publication/pub.1036584248"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89814_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s00018-010-0266-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0266-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0266-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0266-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00018-010-0266-1'


 

This table displays all metadata directly associated to this object as RDF triples.

411 TRIPLES      21 PREDICATES      114 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00018-010-0266-1 schema:about N3d47b0a361004d1badb7d0ef7f433896
2 N409a552dc75f4826b1500010962b5cdd
3 N5b03a52a7e914213bd891128b07c30c9
4 N5d062f1b66ec4d609e24dbc0161358d5
5 N6aeb3d1534af4174ba1c41f8b4d88af1
6 N74b4b6a7198145b29cba7fdaed9ea7f5
7 N819599c2743243e4ad661d1e86973cd4
8 N925066bce05942a0a2ae2d509b48274b
9 Na814498a7e4f42adb03f6727f794f9d7
10 Nab250c2982f3405a80f5e06d95cc532d
11 Nca05ea3f42854fb086998f731ceae3e1
12 Ndd9b87f10a5e482e8fa3bad403380ce4
13 Ne0a883b297364b7d91a145acbcf99191
14 Ne4182a20818c4d15b85480664fdd8b02
15 Ne64a50a5701a474bb48f7623c4379408
16 Nf5ad16b2e35c417e9b68f73a41496de2
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author N5cc2fc4952d1422183b114241b86ba45
20 schema:citation sg:pub.10.1007/s00018-004-4225-6
21 sg:pub.10.1038/363446a0
22 sg:pub.10.1038/381272a0
23 sg:pub.10.1038/nature08231
24 sg:pub.10.1038/nbt.1543
25 sg:pub.10.1038/nbt0709-671a
26 sg:pub.10.1038/ncb834
27 sg:pub.10.1038/nmeth953
28 sg:pub.10.1038/nsb0996-733
29 sg:pub.10.1038/nsb0996-803
30 https://app.dimensions.ai/details/publication/pub.1075339595
31 https://app.dimensions.ai/details/publication/pub.1075455247
32 https://app.dimensions.ai/details/publication/pub.1077093941
33 https://app.dimensions.ai/details/publication/pub.1079500386
34 https://app.dimensions.ai/details/publication/pub.1081682349
35 https://app.dimensions.ai/details/publication/pub.1082095424
36 https://doi.org/10.1002/(sici)1521-4141(199911)29:11<3450::aid-immu3450>3.0.co;2-a
37 https://doi.org/10.1006/jmbi.1993.1351
38 https://doi.org/10.1016/0014-5793(91)80206-i
39 https://doi.org/10.1016/j.canlet.2007.03.023
40 https://doi.org/10.1016/j.jbiotec.2008.02.012
41 https://doi.org/10.1016/j.jsb.2008.11.008
42 https://doi.org/10.1016/j.str.2008.11.011
43 https://doi.org/10.1016/j.vetimm.2008.10.299
44 https://doi.org/10.1016/s0014-5793(97)01062-4
45 https://doi.org/10.1016/s0022-2836(02)01131-2
46 https://doi.org/10.1016/s0092-8674(00)80527-9
47 https://doi.org/10.1016/s0969-2126(99)80049-5
48 https://doi.org/10.1021/bi00156a028
49 https://doi.org/10.1038/sj.emboj.7600280
50 https://doi.org/10.1038/sj.emboj.7600872
51 https://doi.org/10.1042/bj3240283
52 https://doi.org/10.1073/pnas.0505379103
53 https://doi.org/10.1073/pnas.0812374106
54 https://doi.org/10.1073/pnas.76.9.4350
55 https://doi.org/10.1074/jbc.m602567200
56 https://doi.org/10.1083/jcb.102.4.1439
57 https://doi.org/10.1083/jcb.104.3.491
58 https://doi.org/10.1083/jcb.108.5.1717
59 https://doi.org/10.1083/jcb.109.2.593
60 https://doi.org/10.1083/jcb.112.6.1151
61 https://doi.org/10.1083/jcb.116.5.1135
62 https://doi.org/10.1083/jcb.119.6.1451
63 https://doi.org/10.1083/jcb.145.1.109
64 https://doi.org/10.1083/jcb.200101113
65 https://doi.org/10.1084/jem.165.1.97
66 https://doi.org/10.1093/emboj/17.20.5923
67 https://doi.org/10.1093/emboj/17.5.1362
68 https://doi.org/10.1093/emboj/cdf680
69 https://doi.org/10.1093/protein/9.6.531
70 https://doi.org/10.1096/fj.06-6306fje
71 https://doi.org/10.1107/s0907444901014007
72 https://doi.org/10.1107/s0907444903020924
73 https://doi.org/10.1107/s0907444904019158
74 https://doi.org/10.1107/s0907444992007698
75 https://doi.org/10.1107/s0907444994003112
76 https://doi.org/10.1111/j.1432-1033.1993.tb18403.x
77 https://doi.org/10.1111/j.1432-1033.1994.tb20015.x
78 https://doi.org/10.1111/j.1600-0854.2005.00326.x
79 https://doi.org/10.1126/science.1848726
80 https://doi.org/10.1126/science.286.5446.1939
81 https://doi.org/10.1128/aac.45.10.2807-2812.2001
82 https://doi.org/10.1136/gut.2005.083691
83 https://doi.org/10.1152/physrev.00021.2007
84 https://doi.org/10.1161/circresaha.108.172882
85 https://doi.org/10.1161/circresaha.109.196329
86 https://doi.org/10.1261/rna5160904
87 https://doi.org/10.1359/jbmr.040128
88 https://doi.org/10.3233/jad-2009-1127
89 schema:datePublished 2010-05
90 schema:datePublishedReg 2010-05-01
91 schema:description RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated.
92 schema:genre research_article
93 schema:inLanguage en
94 schema:isAccessibleForFree false
95 schema:isPartOf Nc3c0e383730f4a97aab96be79fa5359b
96 Nef0b5eadce9e462cbaa033f4de6457af
97 sg:journal.1295005
98 schema:name A llama-derived gelsolin single-domain antibody blocks gelsolin–G-actin interaction
99 schema:pagination 1519-1535
100 schema:productId N126b9543528b4ba49b611f23f9645864
101 N9d75c62920534224865a34dedd8347eb
102 Nb944679ab2ef4e70a3375e53ab003486
103 Nca91b8ac03504eb2bf539eeb9ed96bc5
104 Nd44413bbc7364e3d9d782192daa5649c
105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036584248
106 https://doi.org/10.1007/s00018-010-0266-1
107 schema:sdDatePublished 2019-04-11T09:59
108 schema:sdLicense https://scigraph.springernature.com/explorer/license/
109 schema:sdPublisher Nd01dc93988724817ba0e0b39a69b239c
110 schema:url http://link.springer.com/10.1007/s00018-010-0266-1
111 sgo:license sg:explorer/license/
112 sgo:sdDataset articles
113 rdf:type schema:ScholarlyArticle
114 N0f8fb39580e743be95b9e1ef8afdbb1b rdf:first sg:person.01273101735.80
115 rdf:rest rdf:nil
116 N126b9543528b4ba49b611f23f9645864 schema:name pubmed_id
117 schema:value 20140750
118 rdf:type schema:PropertyValue
119 N1280d932b3384c18bc96f989e5594a0f rdf:first sg:person.0616250601.02
120 rdf:rest N737186f3598d4d239b63bb3ee1670048
121 N25a2a9fd16c849849724c4c454469966 rdf:first sg:person.01007752627.08
122 rdf:rest N4b0ef8479eff4c29953a1723bffe73fa
123 N2cf12705cd3d4da080852233150d178d rdf:first sg:person.01155503100.24
124 rdf:rest N2fc6cc9633ab4856b1971c87a1b339d9
125 N2fc6cc9633ab4856b1971c87a1b339d9 rdf:first sg:person.01036516654.03
126 rdf:rest N0f8fb39580e743be95b9e1ef8afdbb1b
127 N3d47b0a361004d1badb7d0ef7f433896 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Protein Structure, Tertiary
129 rdf:type schema:DefinedTerm
130 N409a552dc75f4826b1500010962b5cdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Epitopes
132 rdf:type schema:DefinedTerm
133 N4b0ef8479eff4c29953a1723bffe73fa rdf:first sg:person.01030403661.06
134 rdf:rest N2cf12705cd3d4da080852233150d178d
135 N5b03a52a7e914213bd891128b07c30c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Models, Molecular
137 rdf:type schema:DefinedTerm
138 N5cc2fc4952d1422183b114241b86ba45 rdf:first sg:person.01323027521.82
139 rdf:rest N6168c9f04f764bc2bb69894b112b3a38
140 N5d062f1b66ec4d609e24dbc0161358d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Calcium
142 rdf:type schema:DefinedTerm
143 N6168c9f04f764bc2bb69894b112b3a38 rdf:first sg:person.0601624354.44
144 rdf:rest N1280d932b3384c18bc96f989e5594a0f
145 N6aeb3d1534af4174ba1c41f8b4d88af1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Cell Line
147 rdf:type schema:DefinedTerm
148 N737186f3598d4d239b63bb3ee1670048 rdf:first sg:person.0776116714.56
149 rdf:rest Nbe0131a082164323ba641a65a4aa1bf5
150 N74b4b6a7198145b29cba7fdaed9ea7f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Actins
152 rdf:type schema:DefinedTerm
153 N819599c2743243e4ad661d1e86973cd4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Gelsolin
155 rdf:type schema:DefinedTerm
156 N925066bce05942a0a2ae2d509b48274b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Molecular Sequence Data
158 rdf:type schema:DefinedTerm
159 N978ed00cf26743c98b552494967fab29 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
160 schema:familyName Van Loo
161 schema:givenName Berlinda
162 rdf:type schema:Person
163 N9d75c62920534224865a34dedd8347eb schema:name dimensions_id
164 schema:value pub.1036584248
165 rdf:type schema:PropertyValue
166 Na814498a7e4f42adb03f6727f794f9d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Humans
168 rdf:type schema:DefinedTerm
169 Nab250c2982f3405a80f5e06d95cc532d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Single-Chain Antibodies
171 rdf:type schema:DefinedTerm
172 Nb944679ab2ef4e70a3375e53ab003486 schema:name readcube_id
173 schema:value ae45d22f573c1c80c31ce3b93234d6905a9523d7161fc0c67179e84a9bad9a21
174 rdf:type schema:PropertyValue
175 Nbe0131a082164323ba641a65a4aa1bf5 rdf:first N978ed00cf26743c98b552494967fab29
176 rdf:rest N25a2a9fd16c849849724c4c454469966
177 Nc3c0e383730f4a97aab96be79fa5359b schema:issueNumber 9
178 rdf:type schema:PublicationIssue
179 Nca05ea3f42854fb086998f731ceae3e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Mitochondria
181 rdf:type schema:DefinedTerm
182 Nca91b8ac03504eb2bf539eeb9ed96bc5 schema:name nlm_unique_id
183 schema:value 9705402
184 rdf:type schema:PropertyValue
185 Nd01dc93988724817ba0e0b39a69b239c schema:name Springer Nature - SN SciGraph project
186 rdf:type schema:Organization
187 Nd44413bbc7364e3d9d782192daa5649c schema:name doi
188 schema:value 10.1007/s00018-010-0266-1
189 rdf:type schema:PropertyValue
190 Ndd9b87f10a5e482e8fa3bad403380ce4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Camelids, New World
192 rdf:type schema:DefinedTerm
193 Ne0a883b297364b7d91a145acbcf99191 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Crystallography, X-Ray
195 rdf:type schema:DefinedTerm
196 Ne4182a20818c4d15b85480664fdd8b02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name Animals
198 rdf:type schema:DefinedTerm
199 Ne64a50a5701a474bb48f7623c4379408 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Recombinant Fusion Proteins
201 rdf:type schema:DefinedTerm
202 Nef0b5eadce9e462cbaa033f4de6457af schema:volumeNumber 67
203 rdf:type schema:PublicationVolume
204 Nf5ad16b2e35c417e9b68f73a41496de2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
205 schema:name Cell Movement
206 rdf:type schema:DefinedTerm
207 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
208 schema:name Biological Sciences
209 rdf:type schema:DefinedTerm
210 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
211 schema:name Biochemistry and Cell Biology
212 rdf:type schema:DefinedTerm
213 sg:journal.1295005 schema:issn 1420-682X
214 1420-9071
215 schema:name Cellular and Molecular Life Sciences
216 rdf:type schema:Periodical
217 sg:person.01007752627.08 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
218 schema:familyName Soror
219 schema:givenName Sameh Hamdy
220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007752627.08
221 rdf:type schema:Person
222 sg:person.01030403661.06 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
223 schema:familyName Srinivasan
224 schema:givenName Vasundara
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030403661.06
226 rdf:type schema:Person
227 sg:person.01036516654.03 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
228 schema:familyName Vandekerckhove
229 schema:givenName Joël
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036516654.03
231 rdf:type schema:Person
232 sg:person.01155503100.24 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
233 schema:familyName Steyaert
234 schema:givenName Jan
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155503100.24
236 rdf:type schema:Person
237 sg:person.01273101735.80 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
238 schema:familyName Gettemans
239 schema:givenName Jan
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273101735.80
241 rdf:type schema:Person
242 sg:person.01323027521.82 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
243 schema:familyName Van den Abbeele
244 schema:givenName Anske
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323027521.82
246 rdf:type schema:Person
247 sg:person.0601624354.44 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
248 schema:familyName De Clercq
249 schema:givenName Sarah
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601624354.44
251 rdf:type schema:Person
252 sg:person.0616250601.02 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
253 schema:familyName De Ganck
254 schema:givenName Ariane
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616250601.02
256 rdf:type schema:Person
257 sg:person.0776116714.56 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
258 schema:familyName De Corte
259 schema:givenName Veerle
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776116714.56
261 rdf:type schema:Person
262 sg:pub.10.1007/s00018-004-4225-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029653312
263 https://doi.org/10.1007/s00018-004-4225-6
264 rdf:type schema:CreativeWork
265 sg:pub.10.1038/363446a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018109154
266 https://doi.org/10.1038/363446a0
267 rdf:type schema:CreativeWork
268 sg:pub.10.1038/381272a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044595902
269 https://doi.org/10.1038/381272a0
270 rdf:type schema:CreativeWork
271 sg:pub.10.1038/nature08231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029667713
272 https://doi.org/10.1038/nature08231
273 rdf:type schema:CreativeWork
274 sg:pub.10.1038/nbt.1543 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036769338
275 https://doi.org/10.1038/nbt.1543
276 rdf:type schema:CreativeWork
277 sg:pub.10.1038/nbt0709-671a schema:sameAs https://app.dimensions.ai/details/publication/pub.1018408477
278 https://doi.org/10.1038/nbt0709-671a
279 rdf:type schema:CreativeWork
280 sg:pub.10.1038/ncb834 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041500941
281 https://doi.org/10.1038/ncb834
282 rdf:type schema:CreativeWork
283 sg:pub.10.1038/nmeth953 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012346597
284 https://doi.org/10.1038/nmeth953
285 rdf:type schema:CreativeWork
286 sg:pub.10.1038/nsb0996-733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032832494
287 https://doi.org/10.1038/nsb0996-733
288 rdf:type schema:CreativeWork
289 sg:pub.10.1038/nsb0996-803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051868454
290 https://doi.org/10.1038/nsb0996-803
291 rdf:type schema:CreativeWork
292 https://app.dimensions.ai/details/publication/pub.1075339595 schema:CreativeWork
293 https://app.dimensions.ai/details/publication/pub.1075455247 schema:CreativeWork
294 https://app.dimensions.ai/details/publication/pub.1077093941 schema:CreativeWork
295 https://app.dimensions.ai/details/publication/pub.1079500386 schema:CreativeWork
296 https://app.dimensions.ai/details/publication/pub.1081682349 schema:CreativeWork
297 https://app.dimensions.ai/details/publication/pub.1082095424 schema:CreativeWork
298 https://doi.org/10.1002/(sici)1521-4141(199911)29:11<3450::aid-immu3450>3.0.co;2-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1047838040
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1006/jmbi.1993.1351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026297927
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1016/0014-5793(91)80206-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1020925496
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1016/j.canlet.2007.03.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045561525
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1016/j.jbiotec.2008.02.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017983226
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1016/j.jsb.2008.11.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036896722
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1016/j.str.2008.11.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014961753
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1016/j.vetimm.2008.10.299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020528313
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1016/s0014-5793(97)01062-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015552570
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1016/s0022-2836(02)01131-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016067722
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1016/s0092-8674(00)80527-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034781647
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1016/s0969-2126(99)80049-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035578275
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1021/bi00156a028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055163023
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1038/sj.emboj.7600280 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044570454
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1038/sj.emboj.7600872 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021409380
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1042/bj3240283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034328974
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1073/pnas.0505379103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005266742
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1073/pnas.0812374106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005513276
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1073/pnas.76.9.4350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011704795
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1074/jbc.m602567200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025311211
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1083/jcb.102.4.1439 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033081809
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1083/jcb.104.3.491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027471936
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1083/jcb.108.5.1717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038891627
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1083/jcb.109.2.593 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042172228
345 rdf:type schema:CreativeWork
346 https://doi.org/10.1083/jcb.112.6.1151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042903115
347 rdf:type schema:CreativeWork
348 https://doi.org/10.1083/jcb.116.5.1135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030400004
349 rdf:type schema:CreativeWork
350 https://doi.org/10.1083/jcb.119.6.1451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036949715
351 rdf:type schema:CreativeWork
352 https://doi.org/10.1083/jcb.145.1.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047412687
353 rdf:type schema:CreativeWork
354 https://doi.org/10.1083/jcb.200101113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026509682
355 rdf:type schema:CreativeWork
356 https://doi.org/10.1084/jem.165.1.97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053069219
357 rdf:type schema:CreativeWork
358 https://doi.org/10.1093/emboj/17.20.5923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002435548
359 rdf:type schema:CreativeWork
360 https://doi.org/10.1093/emboj/17.5.1362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026367021
361 rdf:type schema:CreativeWork
362 https://doi.org/10.1093/emboj/cdf680 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030008802
363 rdf:type schema:CreativeWork
364 https://doi.org/10.1093/protein/9.6.531 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043661194
365 rdf:type schema:CreativeWork
366 https://doi.org/10.1096/fj.06-6306fje schema:sameAs https://app.dimensions.ai/details/publication/pub.1034913466
367 rdf:type schema:CreativeWork
368 https://doi.org/10.1107/s0907444901014007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009737008
369 rdf:type schema:CreativeWork
370 https://doi.org/10.1107/s0907444903020924 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036336557
371 rdf:type schema:CreativeWork
372 https://doi.org/10.1107/s0907444904019158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020281098
373 rdf:type schema:CreativeWork
374 https://doi.org/10.1107/s0907444992007698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027900257
375 rdf:type schema:CreativeWork
376 https://doi.org/10.1107/s0907444994003112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025573949
377 rdf:type schema:CreativeWork
378 https://doi.org/10.1111/j.1432-1033.1993.tb18403.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006838740
379 rdf:type schema:CreativeWork
380 https://doi.org/10.1111/j.1432-1033.1994.tb20015.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008424189
381 rdf:type schema:CreativeWork
382 https://doi.org/10.1111/j.1600-0854.2005.00326.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009708797
383 rdf:type schema:CreativeWork
384 https://doi.org/10.1126/science.1848726 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062509922
385 rdf:type schema:CreativeWork
386 https://doi.org/10.1126/science.286.5446.1939 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062567537
387 rdf:type schema:CreativeWork
388 https://doi.org/10.1128/aac.45.10.2807-2812.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051826182
389 rdf:type schema:CreativeWork
390 https://doi.org/10.1136/gut.2005.083691 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053322164
391 rdf:type schema:CreativeWork
392 https://doi.org/10.1152/physrev.00021.2007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050366371
393 rdf:type schema:CreativeWork
394 https://doi.org/10.1161/circresaha.108.172882 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030358604
395 rdf:type schema:CreativeWork
396 https://doi.org/10.1161/circresaha.109.196329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050436736
397 rdf:type schema:CreativeWork
398 https://doi.org/10.1261/rna5160904 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021691638
399 rdf:type schema:CreativeWork
400 https://doi.org/10.1359/jbmr.040128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016492035
401 rdf:type schema:CreativeWork
402 https://doi.org/10.3233/jad-2009-1127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077934758
403 rdf:type schema:CreativeWork
404 https://www.grid.ac/institutes/grid.11486.3a schema:alternateName Flanders Institute for Biotechnology
405 schema:name Department of Molecular and Cellular Interactions, VIB, 1050, Brussels, Belgium
406 Structural Biology, Free University of Brussels, Pleinlaan 2, 1050, Brussels, Belgium
407 rdf:type schema:Organization
408 https://www.grid.ac/institutes/grid.5342.0 schema:alternateName Ghent University
409 schema:name Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Albert Baertsoenkaai 3, 9000, Ghent, Belgium
410 Department of Medical Protein Research, VIB, 9000, Ghent, Belgium
411 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...