The effect of tyrosine kinase inhibitors on IgE-mediated histamine release from human lung mast cells and basophils View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-03

AUTHORS

S. E. Lavens-Phillips, E. H. Mockford, J. A. Warner

ABSTRACT

.Objective and Design: To investigate the role of tyrosine kinases (TK) in IgE-mediated signal transduction in human lung mast cells (HLMC) and basophils.¶Materials: Peripheral blood basophils (n ≥ 4) and human lung mast cells (n ≥ 6).¶Treatment: Cells were preincubated with TK inhibitor for 15 min at 37 °C, before the addition of anti-IgE.¶Methods: Histamine release (HR) was assayed using a fluorimetric technique. Results were compared using non-parametric statistics.¶Results: Piceatannol and ST638 significantly (p ≤ 0.05) inhibited anti-IgE induced HR from HLMCs and basophils whilst lavendustin C had no effect in either cell type. Herbimycin A also significantly (p ≤ 0.05) inhibited anti-IgE induced HR from both cell types, an effect which was dose dependent but did require a 16 h preincubation with drug.¶Conclusions: In summary, HLMCs and basophils exhibit distinct inhibitory profiles in the presence of various inhibitors of TK. More... »

PAGES

137-143

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s000110050303

DOI

http://dx.doi.org/10.1007/s000110050303

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015240995

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9562339


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