New endo-β-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-11

AUTHORS

H. Watanabe, K. Nakashima, H. Saito, M. Slaytor

ABSTRACT

. An endo-beta-1,4-glucanase (EG) was purified from the hindgut of an Australian mound-building termite, Coptotermes lacteus. The hindgut extract had a peak separate from those for extracts obtained from the salivary glands and the midgut based on sephacryl S-200 gel chromatography, and also demonstrated an origin different from the endogenous EGs of the termite itself. The recovery was further purified by SDS-PAGE, and its N-terminal amino acid sequence analyzed. This showed high homology to EGs from glycoside hydrolase family (GHF) 7. PCR-based cloning methods were applied to the hindgut contents of C. lacteus and individual protozoan symbionts from C formosanus. cDNAs encoding putative EGs homologous to GHF7 members were then identified. The functionality of one of the putative proteins was confirmed by its expression in Escherichia coli. More... »

PAGES

1983-1992

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/pl00012520

DOI

http://dx.doi.org/10.1007/pl00012520

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041103391

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12530528


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cellulase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Complementary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Eukaryota", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Isoptera", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phylogeny", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Alignment", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Symbiosis", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Institute of Agrobiological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.410590.9", 
          "name": [
            "National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634 (Japan), Fax +81 298 38 6108, e-mail: hinabe@affrc.go.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "H.", 
        "id": "sg:person.01051130235.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051130235.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Division of Environmental Science and Technology, Graduate School of Agricultural Science, Kyoto University, Sakyo-ku, Kyoto 606-8502 (Japan), JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakashima", 
        "givenName": "K.", 
        "id": "sg:person.0627524153.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627524153.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Agrobiological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.410590.9", 
          "name": [
            "National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634 (Japan), Fax +81 298 38 6108, e-mail: hinabe@affrc.go.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saito", 
        "givenName": "H.", 
        "id": "sg:person.01076444234.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076444234.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sydney", 
          "id": "https://www.grid.ac/institutes/grid.1013.3", 
          "name": [
            "Department of Biochemistry, The University of Sydney, Sydney, New South Wales 2006 (Australia), AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Slaytor", 
        "givenName": "M.", 
        "id": "sg:person.01322523616.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322523616.29"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2002-11", 
    "datePublishedReg": "2002-11-01", 
    "description": "Abstract. An endo-beta-1,4-glucanase (EG) was purified from the hindgut of an Australian mound-building termite, Coptotermes lacteus. The hindgut extract had a peak separate from those for extracts obtained from the salivary glands and the midgut based on sephacryl S-200 gel chromatography, and also demonstrated an origin different from the endogenous EGs of the termite itself. The recovery was further purified by SDS-PAGE, and its N-terminal amino acid sequence analyzed. This showed high homology to EGs from glycoside hydrolase family (GHF) 7. PCR-based cloning methods were applied to the hindgut contents of C. lacteus and individual protozoan symbionts from C formosanus. cDNAs encoding putative EGs homologous to GHF7 members were then identified. The functionality of one of the putative proteins was confirmed by its expression in Escherichia coli.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/pl00012520", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1295005", 
        "issn": [
          "1420-682X", 
          "1420-9071"
        ], 
        "name": "Cellular and Molecular Life Sciences", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "11", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "59"
      }
    ], 
    "name": "New endo-\u03b2-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites", 
    "pagination": "1983-1992", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c3cd65ebace934a9a59a3a381f24893b5089319995fedf4245a323d93fa2bc83"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12530528"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9705402"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/pl00012520"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1041103391"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/pl00012520", 
      "https://app.dimensions.ai/details/publication/pub.1041103391"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2FPL00012520"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/pl00012520'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/pl00012520'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/pl00012520'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/pl00012520'


 

This table displays all metadata directly associated to this object as RDF triples.

144 TRIPLES      20 PREDICATES      41 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/pl00012520 schema:about N075580f2e9de49b090f4c54e2288d293
2 N1e15507515f440a9bb4e15de4293999d
3 N219207b206964d478f4e60b2a1a52606
4 N236eb4c8d8454ae1b1d38378bb67a188
5 N3eb547839e4d42eda2e88258834b5cfc
6 N6bdb1d79dc974b60bd7998c02a2446b5
7 N84d565184db94042a0b253e5182749c4
8 N8e8f7d7d44e7468b8609ba09aa9c0ef9
9 N9384f9a4c79042378950d51f49fc7374
10 Na733d1fb56464677877fa4e3e651a0af
11 Nd0bcbf0cdf1a4b5fa6f4187da375ffc7
12 Nebf774a9a71544afa16e262bc67ff075
13 anzsrc-for:06
14 anzsrc-for:0605
15 schema:author N446328e4611d42f5a9529b1de2287622
16 schema:datePublished 2002-11
17 schema:datePublishedReg 2002-11-01
18 schema:description Abstract. An endo-beta-1,4-glucanase (EG) was purified from the hindgut of an Australian mound-building termite, Coptotermes lacteus. The hindgut extract had a peak separate from those for extracts obtained from the salivary glands and the midgut based on sephacryl S-200 gel chromatography, and also demonstrated an origin different from the endogenous EGs of the termite itself. The recovery was further purified by SDS-PAGE, and its N-terminal amino acid sequence analyzed. This showed high homology to EGs from glycoside hydrolase family (GHF) 7. PCR-based cloning methods were applied to the hindgut contents of C. lacteus and individual protozoan symbionts from C formosanus. cDNAs encoding putative EGs homologous to GHF7 members were then identified. The functionality of one of the putative proteins was confirmed by its expression in Escherichia coli.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N5ffb1a1f8beb4d73821d041bc40bdd66
23 Na27ca204d47549dcb693a10114e242d3
24 sg:journal.1295005
25 schema:name New endo-β-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites
26 schema:pagination 1983-1992
27 schema:productId N8fdf7371224e440eb14cd7e5a83481d5
28 Ncf1c64e899c94c9ba1f8df6a4fbd8ff8
29 Ne285f74a16c947ba9aa1dbb4e53715f4
30 Ne304d4c44df34064b5941124ab330320
31 Nf3c446e5a41d4a3eb3fc0c0ac0f03b44
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041103391
33 https://doi.org/10.1007/pl00012520
34 schema:sdDatePublished 2019-04-10T19:58
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher N5c484da7228d44f5b3cb7cbf673b3136
37 schema:url http://link.springer.com/10.1007%2FPL00012520
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N075580f2e9de49b090f4c54e2288d293 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
42 schema:name DNA, Complementary
43 rdf:type schema:DefinedTerm
44 N1e15507515f440a9bb4e15de4293999d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
45 schema:name Cloning, Molecular
46 rdf:type schema:DefinedTerm
47 N219207b206964d478f4e60b2a1a52606 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Polymerase Chain Reaction
49 rdf:type schema:DefinedTerm
50 N2226bf5444e646949a6f3acd4e599888 rdf:first sg:person.01076444234.54
51 rdf:rest Nd5c39b8a388a4c99a2ce07fbcf36c1b6
52 N236eb4c8d8454ae1b1d38378bb67a188 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Symbiosis
54 rdf:type schema:DefinedTerm
55 N3eb547839e4d42eda2e88258834b5cfc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Molecular Sequence Data
57 rdf:type schema:DefinedTerm
58 N446328e4611d42f5a9529b1de2287622 rdf:first sg:person.01051130235.32
59 rdf:rest Nec8b82e4d1364443bc0b40e85eda0947
60 N5c484da7228d44f5b3cb7cbf673b3136 schema:name Springer Nature - SN SciGraph project
61 rdf:type schema:Organization
62 N5ffb1a1f8beb4d73821d041bc40bdd66 schema:volumeNumber 59
63 rdf:type schema:PublicationVolume
64 N6bdb1d79dc974b60bd7998c02a2446b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Eukaryota
66 rdf:type schema:DefinedTerm
67 N84d565184db94042a0b253e5182749c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Cellulase
69 rdf:type schema:DefinedTerm
70 N8e8f7d7d44e7468b8609ba09aa9c0ef9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Animals
72 rdf:type schema:DefinedTerm
73 N8fdf7371224e440eb14cd7e5a83481d5 schema:name pubmed_id
74 schema:value 12530528
75 rdf:type schema:PropertyValue
76 N9384f9a4c79042378950d51f49fc7374 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Amino Acid Sequence
78 rdf:type schema:DefinedTerm
79 Na27ca204d47549dcb693a10114e242d3 schema:issueNumber 11
80 rdf:type schema:PublicationIssue
81 Na733d1fb56464677877fa4e3e651a0af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Sequence Alignment
83 rdf:type schema:DefinedTerm
84 Ncf1c64e899c94c9ba1f8df6a4fbd8ff8 schema:name dimensions_id
85 schema:value pub.1041103391
86 rdf:type schema:PropertyValue
87 Nd0bcbf0cdf1a4b5fa6f4187da375ffc7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Phylogeny
89 rdf:type schema:DefinedTerm
90 Nd5c39b8a388a4c99a2ce07fbcf36c1b6 rdf:first sg:person.01322523616.29
91 rdf:rest rdf:nil
92 Ne285f74a16c947ba9aa1dbb4e53715f4 schema:name nlm_unique_id
93 schema:value 9705402
94 rdf:type schema:PropertyValue
95 Ne304d4c44df34064b5941124ab330320 schema:name readcube_id
96 schema:value c3cd65ebace934a9a59a3a381f24893b5089319995fedf4245a323d93fa2bc83
97 rdf:type schema:PropertyValue
98 Nebf774a9a71544afa16e262bc67ff075 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Isoptera
100 rdf:type schema:DefinedTerm
101 Nec8b82e4d1364443bc0b40e85eda0947 rdf:first sg:person.0627524153.78
102 rdf:rest N2226bf5444e646949a6f3acd4e599888
103 Nf3c446e5a41d4a3eb3fc0c0ac0f03b44 schema:name doi
104 schema:value 10.1007/pl00012520
105 rdf:type schema:PropertyValue
106 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
107 schema:name Biological Sciences
108 rdf:type schema:DefinedTerm
109 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
110 schema:name Microbiology
111 rdf:type schema:DefinedTerm
112 sg:journal.1295005 schema:issn 1420-682X
113 1420-9071
114 schema:name Cellular and Molecular Life Sciences
115 rdf:type schema:Periodical
116 sg:person.01051130235.32 schema:affiliation https://www.grid.ac/institutes/grid.410590.9
117 schema:familyName Watanabe
118 schema:givenName H.
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051130235.32
120 rdf:type schema:Person
121 sg:person.01076444234.54 schema:affiliation https://www.grid.ac/institutes/grid.410590.9
122 schema:familyName Saito
123 schema:givenName H.
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076444234.54
125 rdf:type schema:Person
126 sg:person.01322523616.29 schema:affiliation https://www.grid.ac/institutes/grid.1013.3
127 schema:familyName Slaytor
128 schema:givenName M.
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322523616.29
130 rdf:type schema:Person
131 sg:person.0627524153.78 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
132 schema:familyName Nakashima
133 schema:givenName K.
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627524153.78
135 rdf:type schema:Person
136 https://www.grid.ac/institutes/grid.1013.3 schema:alternateName University of Sydney
137 schema:name Department of Biochemistry, The University of Sydney, Sydney, New South Wales 2006 (Australia), AU
138 rdf:type schema:Organization
139 https://www.grid.ac/institutes/grid.258799.8 schema:alternateName Kyoto University
140 schema:name Division of Environmental Science and Technology, Graduate School of Agricultural Science, Kyoto University, Sakyo-ku, Kyoto 606-8502 (Japan), JP
141 rdf:type schema:Organization
142 https://www.grid.ac/institutes/grid.410590.9 schema:alternateName National Institute of Agrobiological Sciences
143 schema:name National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634 (Japan), Fax +81 298 38 6108, e-mail: hinabe@affrc.go.jp, JP
144 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...