New endo-β-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-11

AUTHORS

H. Watanabe, K. Nakashima, H. Saito, M. Slaytor

ABSTRACT

. An endo-beta-1,4-glucanase (EG) was purified from the hindgut of an Australian mound-building termite, Coptotermes lacteus. The hindgut extract had a peak separate from those for extracts obtained from the salivary glands and the midgut based on sephacryl S-200 gel chromatography, and also demonstrated an origin different from the endogenous EGs of the termite itself. The recovery was further purified by SDS-PAGE, and its N-terminal amino acid sequence analyzed. This showed high homology to EGs from glycoside hydrolase family (GHF) 7. PCR-based cloning methods were applied to the hindgut contents of C. lacteus and individual protozoan symbionts from C formosanus. cDNAs encoding putative EGs homologous to GHF7 members were then identified. The functionality of one of the putative proteins was confirmed by its expression in Escherichia coli. More... »

PAGES

1983-1992

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/pl00012520

DOI

http://dx.doi.org/10.1007/pl00012520

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041103391

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12530528


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