Ontology type: schema:ScholarlyArticle
2000-01
AUTHORSG. Blanco, E. Fernández, M. J. Fernández, A. F. Braña, U. Weissbach, E. Künzel, J. Rohr, C. Méndez, J. A. Salas
ABSTRACTA 2,580-bp region of the chromosome of Streptomyces argillaceus, the producer of the antitumor polyketide mithramycin, was sequenced. Analysis of the nucleotide sequence revealed the presence of two genes (mtmGIII and mtmGIV) encoding proteins that showed a high degree of similarity to glycosyltransferases involved in the biosynthesis of various antibiotics and antitumor drugs. Independent insertional inactivation of both genes produced mutants that did not synthesize mithramycin but accumulated several mithramycin intermediates. Both mutants accumulated premithramycinone, a non-glycosylated intermediate in mithramycin biosynthesis. The mutant affected in the mtmGIII gene also accumulated premithramycin A1, which contains premithramycinone as the aglycon unit and a D-olivose attached at C-12a-O. These experiments demonstrate that the glycosyltransferases MtmGIV and MtmGIII catalyze the first two glycosylation steps in mithramycin biosynthesis. A model is proposed for the glycosylation steps in mithramycin biosynthesis. More... »
PAGES991-1000
http://scigraph.springernature.com/pub.10.1007/pl00008667
DOIhttp://dx.doi.org/10.1007/pl00008667
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1025302800
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/10660060
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antibiotics, Antineoplastic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA, Bacterial",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Targeting",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, Bacterial",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glycosylation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glycosyltransferases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Models, Biological",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutagenesis, Insertional",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Phylogeny",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plicamycin",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sequence Homology, Amino Acid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Streptomyces",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Oviedo",
"id": "https://www.grid.ac/institutes/grid.10863.3c",
"name": [
"Departamento de Biolog\u00eda Funcional e Instituto Universitario de Oncolog\u00eda del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain E-mail: jasf@sauron.quimica.uniovi.es; Fax: +34-985-103652, ES"
],
"type": "Organization"
},
"familyName": "Blanco",
"givenName": "G.",
"id": "sg:person.01267714027.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267714027.48"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Oviedo",
"id": "https://www.grid.ac/institutes/grid.10863.3c",
"name": [
"Departamento de Biolog\u00eda Funcional e Instituto Universitario de Oncolog\u00eda del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain E-mail: jasf@sauron.quimica.uniovi.es; Fax: +34-985-103652, ES"
],
"type": "Organization"
},
"familyName": "Fern\u00e1ndez",
"givenName": "E.",
"id": "sg:person.01276613342.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276613342.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Oviedo",
"id": "https://www.grid.ac/institutes/grid.10863.3c",
"name": [
"Departamento de Biolog\u00eda Funcional e Instituto Universitario de Oncolog\u00eda del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain E-mail: jasf@sauron.quimica.uniovi.es; Fax: +34-985-103652, ES"
],
"type": "Organization"
},
"familyName": "Fern\u00e1ndez",
"givenName": "M. J.",
"id": "sg:person.01276520732.99",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276520732.99"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Oviedo",
"id": "https://www.grid.ac/institutes/grid.10863.3c",
"name": [
"Departamento de Biolog\u00eda Funcional e Instituto Universitario de Oncolog\u00eda del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain E-mail: jasf@sauron.quimica.uniovi.es; Fax: +34-985-103652, ES"
],
"type": "Organization"
},
"familyName": "Bra\u00f1a",
"givenName": "A. F.",
"id": "sg:person.01052707036.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01052707036.01"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Medical University of South Carolina",
"id": "https://www.grid.ac/institutes/grid.259828.c",
"name": [
"Medical University of South Carolina, Department of Pharmaceutical Sciences, 171 Ashley Avenue, Charleston, SC 29425-2303, USA, US"
],
"type": "Organization"
},
"familyName": "Weissbach",
"givenName": "U.",
"id": "sg:person.0753322606.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753322606.50"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Medical University of South Carolina",
"id": "https://www.grid.ac/institutes/grid.259828.c",
"name": [
"Medical University of South Carolina, Department of Pharmaceutical Sciences, 171 Ashley Avenue, Charleston, SC 29425-2303, USA, US"
],
"type": "Organization"
},
"familyName": "K\u00fcnzel",
"givenName": "E.",
"id": "sg:person.01050314225.87",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050314225.87"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Medical University of South Carolina",
"id": "https://www.grid.ac/institutes/grid.259828.c",
"name": [
"Medical University of South Carolina, Department of Pharmaceutical Sciences, 171 Ashley Avenue, Charleston, SC 29425-2303, USA, US"
],
"type": "Organization"
},
"familyName": "Rohr",
"givenName": "J.",
"id": "sg:person.0734556550.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734556550.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Oviedo",
"id": "https://www.grid.ac/institutes/grid.10863.3c",
"name": [
"Departamento de Biolog\u00eda Funcional e Instituto Universitario de Oncolog\u00eda del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain E-mail: jasf@sauron.quimica.uniovi.es; Fax: +34-985-103652, ES"
],
"type": "Organization"
},
"familyName": "M\u00e9ndez",
"givenName": "C.",
"id": "sg:person.0637167675.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637167675.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Oviedo",
"id": "https://www.grid.ac/institutes/grid.10863.3c",
"name": [
"Departamento de Biolog\u00eda Funcional e Instituto Universitario de Oncolog\u00eda del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain E-mail: jasf@sauron.quimica.uniovi.es; Fax: +34-985-103652, ES"
],
"type": "Organization"
},
"familyName": "Salas",
"givenName": "J. A.",
"id": "sg:person.016066717072.74",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016066717072.74"
],
"type": "Person"
}
],
"datePublished": "2000-01",
"datePublishedReg": "2000-01-01",
"description": "A 2,580-bp region of the chromosome of Streptomyces argillaceus, the producer of the antitumor polyketide mithramycin, was sequenced. Analysis of the nucleotide sequence revealed the presence of two genes (mtmGIII and mtmGIV) encoding proteins that showed a high degree of similarity to glycosyltransferases involved in the biosynthesis of various antibiotics and antitumor drugs. Independent insertional inactivation of both genes produced mutants that did not synthesize mithramycin but accumulated several mithramycin intermediates. Both mutants accumulated premithramycinone, a non-glycosylated intermediate in mithramycin biosynthesis. The mutant affected in the mtmGIII gene also accumulated premithramycin A1, which contains premithramycinone as the aglycon unit and a D-olivose attached at C-12a-O. These experiments demonstrate that the glycosyltransferases MtmGIV and MtmGIII catalyze the first two glycosylation steps in mithramycin biosynthesis. A model is proposed for the glycosylation steps in mithramycin biosynthesis.",
"genre": "research_article",
"id": "sg:pub.10.1007/pl00008667",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1297380",
"issn": [
"1617-4615",
"1432-1874"
],
"name": "Molecular Genetics and Genomics",
"type": "Periodical"
},
{
"issueNumber": "6",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "262"
}
],
"name": "Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus",
"pagination": "991-1000",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"788b066e50096891c6857c900b96a812d106c1bfc9503cdd9c240d54d98c1bc0"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"10660060"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0125036"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/pl00008667"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1025302800"
]
}
],
"sameAs": [
"https://doi.org/10.1007/pl00008667",
"https://app.dimensions.ai/details/publication/pub.1025302800"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T19:50",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000481.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/PL00008667"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/pl00008667'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/pl00008667'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/pl00008667'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/pl00008667'
This table displays all metadata directly associated to this object as RDF triples.
192 TRIPLES
20 PREDICATES
45 URIs
37 LITERALS
25 BLANK NODES