Expression of Human Growth Hormone by the Eukaryotic Alga, Chlorella View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-06

AUTHORS

Richard L. Hawkins,, Michi Nakamura

ABSTRACT

A method to use Chlorella to express a recombinant heterologous protein that can be recovered from the extracellular medium has been developed. Plasmids are constructed with an extracellular secretion signal sequence inserted between a promoter region and a gene for human growth hormone (hGH). The plasmids also contain a Kanr region which confers resistance to the antibiotic G418. Protoplasts are prepared by enzymatic treatment, and the plasmid is introduced by incubation of the protoplasts with polyethylene glycol and dimethyl sulfoxide. Cells are then grown in the presence of G418, and the medium is collected from 6 days after transfection. hGH is measured by immunoassay, and values for expressed hGH of about 200-600 ng/ml are obtained. More... »

PAGES

335-341

Journal

TITLE

Current Microbiology

ISSUE

6

VOLUME

38

Author Affiliations

Related Patents

  • Recombinant Microalgae Cells Producing Novel Oils
  • Recombinant Microalgae Including Keto-Acyl Acp Synthase
  • Thioesterases And Cells For Production Of Tailored Oils
  • Variant Thioesterases And Methods Of Use
  • Renewable Diesel And Jet Fuel From Microbial Sources
  • Expression Vector For A Secretion And Detection System
  • Vaccine Against White Spot Syndrome Virus
  • Thioesterases And Cells For Production Of Tailored Oils
  • Methods Of Producing Triacylglyceride Compositions Comprising Tailored Oils
  • Use Of Cellulosic Materials For Cultivation Of Microorganisms
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Tailored Oils
  • Renewable Chemical Production From Novel Fatty Acid Feedstocks
  • Tailored Oils
  • Genetically Engineered Microbial Strains Including Prototheca Lipid Pathway Genes
  • Production Of Hydroxylated Fatty Acids In Prototheca Moriformis
  • Pyrolysis Oil And Other Combustible Compositions From Microbial Biomass
  • Variant Thioesterases And Methods Of Use
  • Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
  • Production Of Oil In Microorganisms
  • Methods For Producing A Triglyceride Composition From Algae
  • Hydroxylated Triacylglycerides
  • Genetically Engineered Microbial Strains Including Prototheca Lipid Pathway Genes
  • Secretion Of Recombinant Polypeptides In The Extracellular Medium Of Diatoms
  • Recombinant Microalgae Cells Producing Novel Oils
  • Genetically Engineered Microorganisms That Metabolize Xylose
  • Ketoacyl Acp Synthase Genes And Uses Thereof
  • Renewable Fuels Produced From Oleaginous Microorganisms
  • Heterotrophic Microalgae Expressing Invertase
  • Nucleic Acids Useful In The Manufacture Of Oil
  • Recombinant Microalgae Including Sucrose Invertase And Thioesterase
  • Recombinant Microalgae Cells Producing Novel Oils
  • Glycerol Feedstock Utilization For Oil-Based Fuel Manufacturing
  • Genetically Engineered Microorganisms That Metabolize Xylose
  • Production Of Oil In Microorganisms
  • Microorganisms Expressing Ketoacyl-Coa Synthase And Uses Thereof
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Structuring Fats And Methods Of Producing Structuring Fats
  • Nucleic Acids Useful In The Manufacture Of Oil
  • Tailored Oils
  • Sucrose Feedstock Utilization For Oil-Based Fuel Manufacturing
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Renewable Chemical Production From Novel Fatty Acid Feedstocks
  • Chlorella Species Containing Exogenous Genes
  • Production Of Oil In Microorganisms
  • Tailored Oils Produced From Recombinant Oleaginous Microorganisms
  • Tailored Oils
  • Genetically Engineered Microbial Strains Including Chlorella Protothecoides Lipid Pathway Genes
  • Tailored Oils
  • Renewable Diesel And Jet Fuel From Microbial Sources
  • Structuring Fats And Methods Of Producing Structuring Fats
  • Tailored Oils
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Tailored Oils
  • Recombinant Microalgae Cells Producing Novel Oils
  • Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
  • Variant Thioesterases And Methods Of Use
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/pl00006813

    DOI

    http://dx.doi.org/10.1007/pl00006813

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014984441

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10341074


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Anti-Bacterial Agents", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Southern", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Western", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chlorella", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chloroquine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cosmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electroporation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gentamicins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hot Temperature", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Human Growth Hormone", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Introns", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Sorting Signals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Toyota Motor Corporation (Japan)", 
              "id": "https://www.grid.ac/institutes/grid.462975.b", 
              "name": [
                "Future Project Division, Toyota Motor Corporation, 1, Toyota-cho, Toyota, Aichi 471-8572, Japan, JP"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hawkins,", 
            "givenName": "Richard L.", 
            "id": "sg:person.0732275062.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732275062.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Toyota Motor Corporation (Japan)", 
              "id": "https://www.grid.ac/institutes/grid.462975.b", 
              "name": [
                "Future Project Division, Toyota Motor Corporation, 1, Toyota-cho, Toyota, Aichi 471-8572, Japan, JP"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nakamura", 
            "givenName": "Michi", 
            "type": "Person"
          }
        ], 
        "datePublished": "1999-06", 
        "datePublishedReg": "1999-06-01", 
        "description": "A method to use Chlorella to express a recombinant heterologous protein that can be recovered from the extracellular medium has been developed. Plasmids are constructed with an extracellular secretion signal sequence inserted between a promoter region and a gene for human growth hormone (hGH). The plasmids also contain a Kanr region which confers resistance to the antibiotic G418. Protoplasts are prepared by enzymatic treatment, and the plasmid is introduced by incubation of the protoplasts with polyethylene glycol and dimethyl sulfoxide. Cells are then grown in the presence of G418, and the medium is collected from 6 days after transfection. hGH is measured by immunoassay, and values for expressed hGH of about 200-600 ng/ml are obtained.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/pl00006813", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1088675", 
            "issn": [
              "0343-8651", 
              "1432-0991"
            ], 
            "name": "Current Microbiology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "38"
          }
        ], 
        "name": "Expression of Human Growth Hormone by the Eukaryotic Alga, Chlorella", 
        "pagination": "335-341", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "4764061b4433fe9650aa61a79a59334236db394397ecb09e85d5be8aed906801"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10341074"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "7808448"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/pl00006813"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1014984441"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/pl00006813", 
          "https://app.dimensions.ai/details/publication/pub.1014984441"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T14:09", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000511.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2FPL00006813"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/pl00006813'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/pl00006813'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/pl00006813'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/pl00006813'


     

    This table displays all metadata directly associated to this object as RDF triples.

    147 TRIPLES      20 PREDICATES      47 URIs      39 LITERALS      27 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/pl00006813 schema:about N04ee0bba4b6f4955be0722d149b42791
    2 N1e5264734a664e569095da7073546b6d
    3 N215cca3677db4334be4aa9c5c773ea4b
    4 N355f01d7e9884367af428f63b4e78606
    5 N676cf15fa4de48359f5a74f2e28940f4
    6 N69092c66727341c7861c086d8207ec44
    7 N7449f164513143ed866df75650560504
    8 N896c8899129a4ea7a46d7d9a5dc44404
    9 N91711f78a3724d50bcc05b19104906e7
    10 N9793dca673434a88867f831cfbe5f0cb
    11 Naeb4e98d303e434b91484f7d0137986c
    12 Nb1d690a6f3d740f5a1a005ad2776e6ca
    13 Nca89c143905244dbb3bdacaba23303ef
    14 Ndfca50cf5dc1476181782654102a0719
    15 Ne40ea95541fc42b8857d777c32bd9334
    16 Ne5d110372353420e9254f73e4ce70746
    17 Nec29104ddecd436ab100d974bc27e4b5
    18 Nf64f5f1640d24ca4870209b23e7d4811
    19 anzsrc-for:06
    20 anzsrc-for:0601
    21 schema:author Na9bf529640e84797872d522fd369f780
    22 schema:datePublished 1999-06
    23 schema:datePublishedReg 1999-06-01
    24 schema:description A method to use Chlorella to express a recombinant heterologous protein that can be recovered from the extracellular medium has been developed. Plasmids are constructed with an extracellular secretion signal sequence inserted between a promoter region and a gene for human growth hormone (hGH). The plasmids also contain a Kanr region which confers resistance to the antibiotic G418. Protoplasts are prepared by enzymatic treatment, and the plasmid is introduced by incubation of the protoplasts with polyethylene glycol and dimethyl sulfoxide. Cells are then grown in the presence of G418, and the medium is collected from 6 days after transfection. hGH is measured by immunoassay, and values for expressed hGH of about 200-600 ng/ml are obtained.
    25 schema:genre research_article
    26 schema:inLanguage en
    27 schema:isAccessibleForFree false
    28 schema:isPartOf N26d7226c36e849d3b5d29f7344b43e45
    29 Nbcfee2162f4d4ac8bd06e947d7007a6b
    30 sg:journal.1088675
    31 schema:name Expression of Human Growth Hormone by the Eukaryotic Alga, Chlorella
    32 schema:pagination 335-341
    33 schema:productId N5f011d07e76d46848770ecb8eb292be1
    34 N62ed4fdf2fa549a08a835f0f6fc1c490
    35 N643fee3416704ddcbafaac69b29865bf
    36 N64c842be4f284595ba25702576fdab58
    37 Nafc36b6ee7f641128adbb7f143f4e362
    38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014984441
    39 https://doi.org/10.1007/pl00006813
    40 schema:sdDatePublished 2019-04-10T14:09
    41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    42 schema:sdPublisher N822e22a259924654a715e1d6bd489d73
    43 schema:url http://link.springer.com/10.1007%2FPL00006813
    44 sgo:license sg:explorer/license/
    45 sgo:sdDataset articles
    46 rdf:type schema:ScholarlyArticle
    47 N04ee0bba4b6f4955be0722d149b42791 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    48 schema:name Promoter Regions, Genetic
    49 rdf:type schema:DefinedTerm
    50 N1e5264734a664e569095da7073546b6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    51 schema:name Cosmids
    52 rdf:type schema:DefinedTerm
    53 N215cca3677db4334be4aa9c5c773ea4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    54 schema:name Gene Expression
    55 rdf:type schema:DefinedTerm
    56 N26d7226c36e849d3b5d29f7344b43e45 schema:issueNumber 6
    57 rdf:type schema:PublicationIssue
    58 N355f01d7e9884367af428f63b4e78606 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    59 schema:name Gentamicins
    60 rdf:type schema:DefinedTerm
    61 N5f011d07e76d46848770ecb8eb292be1 schema:name readcube_id
    62 schema:value 4764061b4433fe9650aa61a79a59334236db394397ecb09e85d5be8aed906801
    63 rdf:type schema:PropertyValue
    64 N62ed4fdf2fa549a08a835f0f6fc1c490 schema:name pubmed_id
    65 schema:value 10341074
    66 rdf:type schema:PropertyValue
    67 N643fee3416704ddcbafaac69b29865bf schema:name nlm_unique_id
    68 schema:value 7808448
    69 rdf:type schema:PropertyValue
    70 N64c842be4f284595ba25702576fdab58 schema:name dimensions_id
    71 schema:value pub.1014984441
    72 rdf:type schema:PropertyValue
    73 N676cf15fa4de48359f5a74f2e28940f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Amino Acid Sequence
    75 rdf:type schema:DefinedTerm
    76 N69092c66727341c7861c086d8207ec44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Anti-Bacterial Agents
    78 rdf:type schema:DefinedTerm
    79 N731d4e89bd72490d94ed57a240d31d1c schema:affiliation https://www.grid.ac/institutes/grid.462975.b
    80 schema:familyName Nakamura
    81 schema:givenName Michi
    82 rdf:type schema:Person
    83 N7449f164513143ed866df75650560504 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Transfection
    85 rdf:type schema:DefinedTerm
    86 N822e22a259924654a715e1d6bd489d73 schema:name Springer Nature - SN SciGraph project
    87 rdf:type schema:Organization
    88 N896c8899129a4ea7a46d7d9a5dc44404 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Blotting, Southern
    90 rdf:type schema:DefinedTerm
    91 N91711f78a3724d50bcc05b19104906e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Molecular Sequence Data
    93 rdf:type schema:DefinedTerm
    94 N9723a2a07cee48239ab46a860eeb90f6 rdf:first N731d4e89bd72490d94ed57a240d31d1c
    95 rdf:rest rdf:nil
    96 N9793dca673434a88867f831cfbe5f0cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Chlorella
    98 rdf:type schema:DefinedTerm
    99 Na9bf529640e84797872d522fd369f780 rdf:first sg:person.0732275062.35
    100 rdf:rest N9723a2a07cee48239ab46a860eeb90f6
    101 Naeb4e98d303e434b91484f7d0137986c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Blotting, Western
    103 rdf:type schema:DefinedTerm
    104 Nafc36b6ee7f641128adbb7f143f4e362 schema:name doi
    105 schema:value 10.1007/pl00006813
    106 rdf:type schema:PropertyValue
    107 Nb1d690a6f3d740f5a1a005ad2776e6ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Chloroquine
    109 rdf:type schema:DefinedTerm
    110 Nbcfee2162f4d4ac8bd06e947d7007a6b schema:volumeNumber 38
    111 rdf:type schema:PublicationVolume
    112 Nca89c143905244dbb3bdacaba23303ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Hot Temperature
    114 rdf:type schema:DefinedTerm
    115 Ndfca50cf5dc1476181782654102a0719 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Plasmids
    117 rdf:type schema:DefinedTerm
    118 Ne40ea95541fc42b8857d777c32bd9334 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Protein Sorting Signals
    120 rdf:type schema:DefinedTerm
    121 Ne5d110372353420e9254f73e4ce70746 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Introns
    123 rdf:type schema:DefinedTerm
    124 Nec29104ddecd436ab100d974bc27e4b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Electroporation
    126 rdf:type schema:DefinedTerm
    127 Nf64f5f1640d24ca4870209b23e7d4811 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Human Growth Hormone
    129 rdf:type schema:DefinedTerm
    130 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    131 schema:name Biological Sciences
    132 rdf:type schema:DefinedTerm
    133 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    134 schema:name Biochemistry and Cell Biology
    135 rdf:type schema:DefinedTerm
    136 sg:journal.1088675 schema:issn 0343-8651
    137 1432-0991
    138 schema:name Current Microbiology
    139 rdf:type schema:Periodical
    140 sg:person.0732275062.35 schema:affiliation https://www.grid.ac/institutes/grid.462975.b
    141 schema:familyName Hawkins,
    142 schema:givenName Richard L.
    143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732275062.35
    144 rdf:type schema:Person
    145 https://www.grid.ac/institutes/grid.462975.b schema:alternateName Toyota Motor Corporation (Japan)
    146 schema:name Future Project Division, Toyota Motor Corporation, 1, Toyota-cho, Toyota, Aichi 471-8572, Japan, JP
    147 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...