Expression of Human Growth Hormone by the Eukaryotic Alga, Chlorella View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-06

AUTHORS

Richard L. Hawkins,, Michi Nakamura

ABSTRACT

A method to use Chlorella to express a recombinant heterologous protein that can be recovered from the extracellular medium has been developed. Plasmids are constructed with an extracellular secretion signal sequence inserted between a promoter region and a gene for human growth hormone (hGH). The plasmids also contain a Kanr region which confers resistance to the antibiotic G418. Protoplasts are prepared by enzymatic treatment, and the plasmid is introduced by incubation of the protoplasts with polyethylene glycol and dimethyl sulfoxide. Cells are then grown in the presence of G418, and the medium is collected from 6 days after transfection. hGH is measured by immunoassay, and values for expressed hGH of about 200-600 ng/ml are obtained. More... »

PAGES

335-341

Journal

TITLE

Current Microbiology

ISSUE

6

VOLUME

38

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/pl00006813

    DOI

    http://dx.doi.org/10.1007/pl00006813

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014984441

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10341074


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