Ontology type: schema:ScholarlyArticle
1997-03
AUTHORS ABSTRACTThe efficiency of two cell-transplantation methods were compared for the production of embryonic cell derived fish chimeras. The classic microinjection technique (blastula stage donor cells are microinjected into blastula embryos) was compared to a novel aggregation method for fish developed by our group. This method utilises the ability of dechorionated fish embryos to aggregate. Morula cells dissociated in Ca2+, Mg2+ free medium were aggregated to recipient embryos of different developmental stage.Donor cells and recipient embryos of different developmental stages were used for most efficient incorporation in the chimera. The fate of donor cells derived from blastulae, was followed by labelling them with FITC-dextrane (FD). The most efficient transplantations were gained by using 16–32 cell stage recipients for aggregation (18% survival of chimeras at swim up stage). Labelled donor cells were contributing to the embryos in varying ratio.A comparison of the efficiency of aggregation was made between diploid-diploid, diploid-haploid and diploid-interspecific (diploid) hybrid chimeras. In all three cases chimeras containing different proportion of donor cells were gained. After one day incubation the embryos were dissociated by trypsin digestion and number of labelled and non-labelled cells were counted under fluorescent microscope.Experiments were performed on Rosy barb (Barbus conchonius), Carp (Cyprinus carpio) and African catfish (Clarias gariepinus). More... »
PAGES95-104
http://scigraph.springernature.com/pub.10.1007/bf03543178
DOIhttp://dx.doi.org/10.1007/bf03543178
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1083108522
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/9199704
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals, Genetically Modified",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Blastocyst",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carps",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Catfishes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Aggregation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Transplantation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chimera",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cyprinidae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Dextrans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Diploidy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Fishes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Fluorescein-5-isothiocyanate",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Haploidy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hybridization, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Methods",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microinjections",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Species Specificity",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "G\u00f6d\u00f6ll\u0151, University of Agricultural Sciences, Institute of Animal Husbandry, H-2103, G\u00f6d\u00f6ll\u0151, Hungary",
"id": "http://www.grid.ac/institutes/grid.129553.9",
"name": [
"G\u00f6d\u00f6ll\u0151, University of Agricultural Sciences, Institute of Animal Husbandry, H-2103, G\u00f6d\u00f6ll\u0151, Hungary"
],
"type": "Organization"
},
"familyName": "V\u00e1radi",
"givenName": "L.",
"id": "sg:person.01177441513.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177441513.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "G\u00f6d\u00f6ll\u0151, University of Agricultural Sciences, Institute of Animal Husbandry, H-2103, G\u00f6d\u00f6ll\u0151, Hungary",
"id": "http://www.grid.ac/institutes/grid.129553.9",
"name": [
"G\u00f6d\u00f6ll\u0151, University of Agricultural Sciences, Institute of Animal Husbandry, H-2103, G\u00f6d\u00f6ll\u0151, Hungary"
],
"type": "Organization"
},
"familyName": "Horv\u00e1th",
"givenName": "L.",
"id": "sg:person.012262476147.78",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012262476147.78"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/291293a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025779656",
"https://doi.org/10.1038/291293a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/292154a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017487986",
"https://doi.org/10.1038/292154a0"
],
"type": "CreativeWork"
}
],
"datePublished": "1997-03",
"datePublishedReg": "1997-03-01",
"description": "The efficiency of two cell-transplantation methods were compared for the production of embryonic cell derived fish chimeras. The classic microinjection technique (blastula stage donor cells are microinjected into blastula embryos) was compared to a novel aggregation method for fish developed by our group. This method utilises the ability of dechorionated fish embryos to aggregate. Morula cells dissociated in Ca2+, Mg2+ free medium were aggregated to recipient embryos of different developmental stage.Donor cells and recipient embryos of different developmental stages were used for most efficient incorporation in the chimera. The fate of donor cells derived from blastulae, was followed by labelling them with FITC-dextrane (FD). The most efficient transplantations were gained by using 16\u201332 cell stage recipients for aggregation (18% survival of chimeras at swim up stage). Labelled donor cells were contributing to the embryos in varying ratio.A comparison of the efficiency of aggregation was made between diploid-diploid, diploid-haploid and diploid-interspecific (diploid) hybrid chimeras. In all three cases chimeras containing different proportion of donor cells were gained. After one day incubation the embryos were dissociated by trypsin digestion and number of labelled and non-labelled cells were counted under fluorescent microscope.Experiments were performed on Rosy barb (Barbus conchonius), Carp (Cyprinus carpio) and African catfish (Clarias gariepinus).",
"genre": "article",
"id": "sg:pub.10.1007/bf03543178",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1016905",
"issn": [
"2676-8615",
"2676-8607"
],
"name": "Biologia Futura",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "48"
}
],
"keywords": [
"different developmental stages",
"embryonic cells",
"developmental stages",
"donor cells",
"Labeled donor cells",
"fish embryos",
"efficiency of aggregation",
"rosy barb",
"embryos",
"chimeras",
"trypsin digestion",
"microinjection technique",
"morula cells",
"efficient incorporation",
"cells",
"non-labeled cells",
"fluorescent microscope",
"African catfish",
"efficient transplantation",
"cell transplantation methods",
"blastula",
"days incubation",
"fish",
"free medium",
"aggregation",
"carp",
"catfish",
"fate",
"production",
"barbs",
"digestion",
"Ca2",
"stage",
"incubation",
"Mg2",
"different proportions",
"ability",
"incorporation",
"medium",
"proportion",
"number",
"experiments",
"efficiency",
"comparison",
"microscope",
"group",
"method",
"aggregation method",
"novel aggregation method",
"ratio",
"transplantation",
"technique",
"recipients"
],
"name": "Production of fish chimeras from embryonic cells",
"pagination": "95-104",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1083108522"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf03543178"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"9199704"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf03543178",
"https://app.dimensions.ai/details/publication/pub.1083108522"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-10T09:46",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_274.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/bf03543178"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf03543178'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf03543178'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf03543178'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf03543178'
This table displays all metadata directly associated to this object as RDF triples.
202 TRIPLES
22 PREDICATES
100 URIs
90 LITERALS
25 BLANK NODES