Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-04

AUTHORS

Kazunori Aoki, Christopher Barker, Xavier Danthinne, Michael J. Imperiale, Gary J. Nabel

ABSTRACT

BACKGROUND: Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system. MATERIALS AND METHODS: Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA. RESULTS: After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus. CONCLUSION: This system facilitates the development of recombinant adenoviral vectors for basic and clinical research. More... »

PAGES

224-231

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf03402119

DOI

http://dx.doi.org/10.1007/bf03402119

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1074507569

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10448644


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenoviridae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cosmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytopathogenic Effect, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Integrases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lac Operon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscle, Smooth", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombination, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Swine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Michigan Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412590.b", 
          "name": [
            "Department of Internal Medicine and Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, 1150 West Medical Center Drive, 4520 MSRB I, 48109-0650, Ann Arbor, Michigan, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aoki", 
        "givenName": "Kazunori", 
        "id": "sg:person.01320560333.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320560333.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Michigan Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412590.b", 
          "name": [
            "Department of Internal Medicine and Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, 1150 West Medical Center Drive, 4520 MSRB I, 48109-0650, Ann Arbor, Michigan, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Barker", 
        "givenName": "Christopher", 
        "id": "sg:person.0735624332.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735624332.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Boise VA Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.413845.f", 
          "name": [
            "Research Service 1 51, VA Medical Center, 500 W. Fort Street, Boise, Idaho, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Danthinne", 
        "givenName": "Xavier", 
        "id": "sg:person.0744765636.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744765636.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Michigan Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412590.b", 
          "name": [
            "Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, Michigan, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Imperiale", 
        "givenName": "Michael J.", 
        "id": "sg:person.01077250273.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077250273.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Michigan Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412590.b", 
          "name": [
            "Department of Internal Medicine and Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, 1150 West Medical Center Drive, 4520 MSRB I, 48109-0650, Ann Arbor, Michigan, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nabel", 
        "givenName": "Gary J.", 
        "id": "sg:person.014461217144.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014461217144.35"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.89.17.7905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002274336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.13.6186", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003131106"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.81.4.1026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003216239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(90)80287-s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005912772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(88)90302-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008116386"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci115902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012201951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(76)90042-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017165445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.79.11.3398", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018688839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9525(93)90104-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019985929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng0393-219", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024908449", 
          "https://doi.org/10.1038/ng0393-219"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.4.1414", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028135533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(83)90311-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031272512"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9525(93)90074-r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036856585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.5.2509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044133693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(92)90623-r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044142134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(85)90224-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045403166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(83)80284-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046218244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0959-8049(94)90477-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052448162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90644-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053553056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.1997.8.11-1321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059271885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/21.9.2025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059931800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2017680", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062518414"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8016642", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062651964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1985.tb04158.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079503211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080754057", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082433124", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082903989", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-04", 
    "datePublishedReg": "1999-04-01", 
    "description": "BACKGROUND: Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system.\nMATERIALS AND METHODS: Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA.\nRESULTS: After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus.\nCONCLUSION: This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf03402119", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2511191", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1113628", 
        "issn": [
          "1076-1551", 
          "1528-3658"
        ], 
        "name": "Molecular Medicine", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro", 
    "pagination": "224-231", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "167e7e1f0f8c73272f3649fc82abe07037b9d9860c203aa757c2cd5a12f3d7c4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10448644"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9501023"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf03402119"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1074507569"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf03402119", 
      "https://app.dimensions.ai/details/publication/pub.1074507569"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000525.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2FBF03402119"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf03402119'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf03402119'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf03402119'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf03402119'


 

This table displays all metadata directly associated to this object as RDF triples.

246 TRIPLES      21 PREDICATES      72 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf03402119 schema:about N30e4ff4bb47c4ff2867e084e1ff3cad1
2 N32d6879c3a0a4199bec55e5a07430d25
3 N378a359d3da14dc991551eae334f4be3
4 N46ac592b66bd4065a46ffad17821a42a
5 N6182152966724cf48ffa61fbd8b381df
6 N6677be8f28744a23baa2318b04bd0386
7 N89ca966657c04f5e89a3db933e958795
8 N8c893f7b6ebc4f3b8a5e45d54a9c034f
9 Na6e3d543455247eeb6b451daf8ea5de1
10 Ncab63a9003d943dc923e3dcaab682752
11 Ne5869a1cab0c48a1bfd16f31dc2ac6de
12 Ne84ef84d77124b1ebae4c5409805f6bc
13 Nedbb385095b246b5b64eee4cf6805c88
14 Nf3ebd9ab97cc4581b35c02604780b816
15 Nf61cde3d46b54cf7bd8204861e550e9b
16 Nfe5445f95743444ba3256e5d32d730a8
17 anzsrc-for:06
18 anzsrc-for:0604
19 schema:author Ne2904e81e47546e6bc44e5e8ae04d0e2
20 schema:citation sg:pub.10.1038/ng0393-219
21 https://app.dimensions.ai/details/publication/pub.1080754057
22 https://app.dimensions.ai/details/publication/pub.1082433124
23 https://app.dimensions.ai/details/publication/pub.1082903989
24 https://doi.org/10.1002/j.1460-2075.1985.tb04158.x
25 https://doi.org/10.1016/0014-5793(90)80287-s
26 https://doi.org/10.1016/0022-2836(85)90224-4
27 https://doi.org/10.1016/0022-2836(92)90623-r
28 https://doi.org/10.1016/0042-6822(76)90042-8
29 https://doi.org/10.1016/0042-6822(88)90302-9
30 https://doi.org/10.1016/0092-8674(83)90311-2
31 https://doi.org/10.1016/0092-8674(93)90644-6
32 https://doi.org/10.1016/0168-9525(93)90074-r
33 https://doi.org/10.1016/0168-9525(93)90104-p
34 https://doi.org/10.1016/0959-8049(94)90477-4
35 https://doi.org/10.1016/s0022-2836(83)80284-8
36 https://doi.org/10.1073/pnas.79.11.3398
37 https://doi.org/10.1073/pnas.81.4.1026
38 https://doi.org/10.1073/pnas.89.17.7905
39 https://doi.org/10.1073/pnas.91.13.6186
40 https://doi.org/10.1073/pnas.94.4.1414
41 https://doi.org/10.1073/pnas.95.5.2509
42 https://doi.org/10.1089/hum.1997.8.11-1321
43 https://doi.org/10.1093/nar/21.9.2025
44 https://doi.org/10.1126/science.2017680
45 https://doi.org/10.1126/science.8016642
46 https://doi.org/10.1172/jci115902
47 schema:datePublished 1999-04
48 schema:datePublishedReg 1999-04-01
49 schema:description BACKGROUND: Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system. MATERIALS AND METHODS: Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA. RESULTS: After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus. CONCLUSION: This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf N9cf4f98372ab47bab0bcb80c1fed3b06
54 Nb2e711ecc0a84941bd6a990679a0843f
55 sg:journal.1113628
56 schema:name Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro
57 schema:pagination 224-231
58 schema:productId N087e1bc73c12419c90140a0414082760
59 N34a94821d76c476890bd0792d902fe25
60 N4c805288c86f4963a723c258ef7d1f2a
61 N6009cc225ac64cd6a47a7070631ddd70
62 Ne70f64b36785409f95290d91961da6bb
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074507569
64 https://doi.org/10.1007/bf03402119
65 schema:sdDatePublished 2019-04-10T23:28
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher N4acb9a40c4e34fb382fad0a9eab0c77e
68 schema:url http://link.springer.com/10.1007%2FBF03402119
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N087e1bc73c12419c90140a0414082760 schema:name pubmed_id
73 schema:value 10448644
74 rdf:type schema:PropertyValue
75 N230c17a47f784a398dff755282daa131 rdf:first sg:person.014461217144.35
76 rdf:rest rdf:nil
77 N30e4ff4bb47c4ff2867e084e1ff3cad1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Cytopathogenic Effect, Viral
79 rdf:type schema:DefinedTerm
80 N32d6879c3a0a4199bec55e5a07430d25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Cosmids
82 rdf:type schema:DefinedTerm
83 N34a94821d76c476890bd0792d902fe25 schema:name nlm_unique_id
84 schema:value 9501023
85 rdf:type schema:PropertyValue
86 N378a359d3da14dc991551eae334f4be3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Animals
88 rdf:type schema:DefinedTerm
89 N395525fdda464412b824cbade0af8830 rdf:first sg:person.0744765636.04
90 rdf:rest Na2493e9c284e4bd1b5a7cc8cea6957b0
91 N46ac592b66bd4065a46ffad17821a42a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Integrases
93 rdf:type schema:DefinedTerm
94 N4acb9a40c4e34fb382fad0a9eab0c77e schema:name Springer Nature - SN SciGraph project
95 rdf:type schema:Organization
96 N4c805288c86f4963a723c258ef7d1f2a schema:name dimensions_id
97 schema:value pub.1074507569
98 rdf:type schema:PropertyValue
99 N6009cc225ac64cd6a47a7070631ddd70 schema:name readcube_id
100 schema:value 167e7e1f0f8c73272f3649fc82abe07037b9d9860c203aa757c2cd5a12f3d7c4
101 rdf:type schema:PropertyValue
102 N6182152966724cf48ffa61fbd8b381df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Viral Proteins
104 rdf:type schema:DefinedTerm
105 N6677be8f28744a23baa2318b04bd0386 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Swine
107 rdf:type schema:DefinedTerm
108 N89ca966657c04f5e89a3db933e958795 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Genetic Vectors
110 rdf:type schema:DefinedTerm
111 N8c893f7b6ebc4f3b8a5e45d54a9c034f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Lac Operon
113 rdf:type schema:DefinedTerm
114 N9cf4f98372ab47bab0bcb80c1fed3b06 schema:volumeNumber 5
115 rdf:type schema:PublicationVolume
116 Na2493e9c284e4bd1b5a7cc8cea6957b0 rdf:first sg:person.01077250273.83
117 rdf:rest N230c17a47f784a398dff755282daa131
118 Na6e3d543455247eeb6b451daf8ea5de1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Plasmids
120 rdf:type schema:DefinedTerm
121 Na920393107b84227a6eeff3cd79d04f2 rdf:first sg:person.0735624332.04
122 rdf:rest N395525fdda464412b824cbade0af8830
123 Nb2e711ecc0a84941bd6a990679a0843f schema:issueNumber 4
124 rdf:type schema:PublicationIssue
125 Ncab63a9003d943dc923e3dcaab682752 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Adenoviridae
127 rdf:type schema:DefinedTerm
128 Ne2904e81e47546e6bc44e5e8ae04d0e2 rdf:first sg:person.01320560333.86
129 rdf:rest Na920393107b84227a6eeff3cd79d04f2
130 Ne5869a1cab0c48a1bfd16f31dc2ac6de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Genes, Reporter
132 rdf:type schema:DefinedTerm
133 Ne70f64b36785409f95290d91961da6bb schema:name doi
134 schema:value 10.1007/bf03402119
135 rdf:type schema:PropertyValue
136 Ne84ef84d77124b1ebae4c5409805f6bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Recombination, Genetic
138 rdf:type schema:DefinedTerm
139 Nedbb385095b246b5b64eee4cf6805c88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Transfection
141 rdf:type schema:DefinedTerm
142 Nf3ebd9ab97cc4581b35c02604780b816 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Cells, Cultured
144 rdf:type schema:DefinedTerm
145 Nf61cde3d46b54cf7bd8204861e550e9b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name DNA, Viral
147 rdf:type schema:DefinedTerm
148 Nfe5445f95743444ba3256e5d32d730a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Muscle, Smooth
150 rdf:type schema:DefinedTerm
151 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
152 schema:name Biological Sciences
153 rdf:type schema:DefinedTerm
154 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
155 schema:name Genetics
156 rdf:type schema:DefinedTerm
157 sg:grant.2511191 http://pending.schema.org/fundedItem sg:pub.10.1007/bf03402119
158 rdf:type schema:MonetaryGrant
159 sg:journal.1113628 schema:issn 1076-1551
160 1528-3658
161 schema:name Molecular Medicine
162 rdf:type schema:Periodical
163 sg:person.01077250273.83 schema:affiliation https://www.grid.ac/institutes/grid.412590.b
164 schema:familyName Imperiale
165 schema:givenName Michael J.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077250273.83
167 rdf:type schema:Person
168 sg:person.01320560333.86 schema:affiliation https://www.grid.ac/institutes/grid.412590.b
169 schema:familyName Aoki
170 schema:givenName Kazunori
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320560333.86
172 rdf:type schema:Person
173 sg:person.014461217144.35 schema:affiliation https://www.grid.ac/institutes/grid.412590.b
174 schema:familyName Nabel
175 schema:givenName Gary J.
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014461217144.35
177 rdf:type schema:Person
178 sg:person.0735624332.04 schema:affiliation https://www.grid.ac/institutes/grid.412590.b
179 schema:familyName Barker
180 schema:givenName Christopher
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735624332.04
182 rdf:type schema:Person
183 sg:person.0744765636.04 schema:affiliation https://www.grid.ac/institutes/grid.413845.f
184 schema:familyName Danthinne
185 schema:givenName Xavier
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744765636.04
187 rdf:type schema:Person
188 sg:pub.10.1038/ng0393-219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024908449
189 https://doi.org/10.1038/ng0393-219
190 rdf:type schema:CreativeWork
191 https://app.dimensions.ai/details/publication/pub.1080754057 schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1082433124 schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1082903989 schema:CreativeWork
194 https://doi.org/10.1002/j.1460-2075.1985.tb04158.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079503211
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/0014-5793(90)80287-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1005912772
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/0022-2836(85)90224-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045403166
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/0022-2836(92)90623-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1044142134
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/0042-6822(76)90042-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017165445
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0042-6822(88)90302-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008116386
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0092-8674(83)90311-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031272512
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0092-8674(93)90644-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053553056
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/0168-9525(93)90074-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1036856585
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0168-9525(93)90104-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1019985929
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0959-8049(94)90477-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052448162
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/s0022-2836(83)80284-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046218244
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1073/pnas.79.11.3398 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018688839
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1073/pnas.81.4.1026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003216239
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1073/pnas.89.17.7905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002274336
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1073/pnas.91.13.6186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003131106
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1073/pnas.94.4.1414 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028135533
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1073/pnas.95.5.2509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044133693
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1089/hum.1997.8.11-1321 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059271885
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1093/nar/21.9.2025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059931800
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1126/science.2017680 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062518414
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1126/science.8016642 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062651964
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1172/jci115902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012201951
239 rdf:type schema:CreativeWork
240 https://www.grid.ac/institutes/grid.412590.b schema:alternateName Michigan Medicine
241 schema:name Department of Internal Medicine and Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, 1150 West Medical Center Drive, 4520 MSRB I, 48109-0650, Ann Arbor, Michigan, USA
242 Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, Michigan, USA
243 rdf:type schema:Organization
244 https://www.grid.ac/institutes/grid.413845.f schema:alternateName Boise VA Medical Center
245 schema:name Research Service 1 51, VA Medical Center, 500 W. Fort Street, Boise, Idaho, USA
246 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...