Prevalence of Four Virulence Genes in Campylobacter jejuni Determined by PCR and Sequence Analysis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-12

AUTHORS

Vasilios Kordinas, Chryssoula Nicolaou, Anastassios Ioannidis, Eleni Papavasileiou, Nicolaos John Legakis, Stylianos Chatzipanagiotou

ABSTRACT

INTRODUCTION: The presence of four virulence genes (racR, wlaN, cgtB, virB11) in 356 Campylobacter jejuni strains isolated from confirmed clinical cases was examined by PCR and sequence analysis. The investigated genes were chosen on the basis of their variation in prevalence. METHODS: The virulence genes were detected by PCR and the amplified products were submitted for sequence analysis. RESULTS: The gene with the highest prevalence was racR (87.08%). virB was present in only 1.69% of the C. jejuni strains, and wlaN and cgtB were detected in 16.01% and 24.44%, respectively. Five strains associated with Guillain-Barré syndrome and Miller-Fischer syndrome out of the total of 356 (1.40%) were positive for cgtB. CONCLUSION: Our findings suggest that racR may encode factors necessary for bacterial pathogenicity in humans, while the roles of the other three genes remain ambiguous. More... »

PAGES

211-215

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf03260094

DOI

http://dx.doi.org/10.1007/bf03260094

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036416971

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16392901


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