Artificial dermis composed of gelatin, hyaluronic acid and (1→3),(1→6)-β-glucan View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-10

AUTHORS

Sang Bong Lee, Hyun Wook Jeon, Young Woo Lee, Seong Kwan Cho, Young Moo Lee, Kang Won Song, Moon Hyang Park, Sung Hwa Hong

ABSTRACT

Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and β-glucan were prepared by using the freeze-drying method after cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a childs foreskin, and cultured in gelatin-based scaffolds. Anin vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week. More... »

PAGES

368-374

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf03218378

DOI

http://dx.doi.org/10.1007/bf03218378

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019457997


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